##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548841_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	646928
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16666769717805	31.0	31.0	34.0	28.0	34.0
2	31.37362890460762	31.0	31.0	34.0	30.0	34.0
3	31.26849664877699	31.0	31.0	34.0	28.0	34.0
4	35.24966302277842	37.0	35.0	37.0	33.0	37.0
5	34.89975855118344	35.0	35.0	37.0	32.0	37.0
6	34.928188299161576	35.0	35.0	37.0	32.0	37.0
7	34.75839196943091	35.0	35.0	37.0	32.0	37.0
8	35.13238412930033	37.0	35.0	37.0	32.0	37.0
9	35.82563902010734	39.0	35.0	39.0	32.0	39.0
10	35.734301189622336	37.0	35.0	39.0	30.0	39.0
11	36.074357579205106	37.0	35.0	39.0	31.0	39.0
12	35.598017090000745	37.0	35.0	39.0	30.0	39.0
13	35.44721669181114	37.0	35.0	39.0	30.0	39.0
14	36.351287933123935	38.0	35.0	40.0	31.0	41.0
15	36.50123661365716	38.0	35.0	40.0	31.0	41.0
16	36.376743625256594	38.0	35.0	40.0	31.0	41.0
17	36.36206965844731	38.0	35.0	40.0	31.0	41.0
18	36.06689770731828	38.0	35.0	40.0	30.0	41.0
19	36.05391326391808	38.0	34.0	40.0	30.0	41.0
20	36.1777322978755	38.0	34.0	40.0	30.0	41.0
21	36.28859007493879	38.0	34.0	40.0	31.0	41.0
22	36.28726226102441	38.0	35.0	40.0	31.0	41.0
23	36.21615697573764	38.0	35.0	40.0	31.0	41.0
24	35.821326329977985	38.0	34.0	40.0	30.0	41.0
25	35.92788223728143	38.0	34.0	40.0	30.0	41.0
26	35.83733738530408	38.0	34.0	40.0	30.0	41.0
27	35.806862587490414	38.0	34.0	40.0	30.0	41.0
28	35.750111295229146	38.0	34.0	40.0	30.0	41.0
29	35.56771696386615	37.0	34.0	40.0	29.0	41.0
30	35.56995987188682	37.0	34.0	40.0	29.0	41.0
31	35.4741671407019	37.0	34.0	40.0	29.0	41.0
32	35.38134382806124	37.0	34.0	40.0	29.0	41.0
33	35.32847550268345	37.0	34.0	40.0	29.0	41.0
34	35.34205815794029	37.0	34.0	40.0	29.0	41.0
35	35.07360942794252	37.0	34.0	40.0	27.0	41.0
36	35.07340693245616	37.0	34.0	40.0	27.0	41.0
37	34.867421104048674	37.0	34.0	40.0	26.0	41.0
38	34.80317747879207	37.0	34.0	40.0	26.0	41.0
39	34.70616822892192	37.0	34.0	40.0	26.0	41.0
40	34.31818996859001	36.0	33.0	40.0	24.0	41.0
41	34.5471335295427	37.0	33.0	40.0	25.0	41.0
42	34.41687173843148	36.0	33.0	40.0	25.0	41.0
43	34.47433717507976	37.0	33.0	40.0	25.0	41.0
44	34.442296515222715	36.0	33.0	40.0	25.0	41.0
45	34.125154576707146	36.0	33.0	40.0	24.0	41.0
46	33.912372010486486	35.0	33.0	39.0	23.0	40.0
47	33.71406555288996	35.0	33.0	39.0	23.0	40.0
48	33.675494027156034	35.0	33.0	39.0	23.0	40.0
49	33.52688707244083	35.0	33.0	39.0	22.0	40.0
50	33.35314749091089	35.0	32.0	39.0	22.0	40.0
51	31.289628830410802	34.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	9.0
10	12.0
11	13.0
12	10.0
13	18.0
14	36.0
15	55.0
16	142.0
17	302.0
18	569.0
19	1079.0
20	1716.0
21	2716.0
22	3712.0
23	4725.0
24	5527.0
25	6289.0
26	7347.0
27	9046.0
28	11368.0
29	14651.0
30	19381.0
31	25582.0
32	33639.0
33	46065.0
34	72025.0
35	75086.0
36	68965.0
37	84276.0
38	95380.0
39	57181.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.844335072836547	41.416046298815324	17.025542255088666	23.714076373259466
2	20.692874632107436	41.50523705883808	17.956248608809634	19.84563970024485
3	19.161946924541834	28.59081690698192	29.701759701234142	22.545476467242104
4	27.26485791309079	28.99147973190216	17.516632453688818	26.227029901318232
5	16.102101006603515	40.286554299705685	18.095058491825984	25.516286201864812
6	20.93447802537531	33.6819553335147	26.388253406870625	18.995313234239358
7	69.20105483144955	15.524138698587786	9.060513689313185	6.21429278064947
8	71.66871738431479	6.409523161773799	6.203008681027873	15.718750772883535
9	69.72568817550021	11.187643756337645	11.500661588306581	7.586006479855563
10	42.76859248633542	26.134438453738284	14.535156926273093	16.561812133653202
11	34.8766787030396	22.930681621447828	22.79156258501719	19.401077090495384
12	30.405083718744592	23.16950263398709	31.18615981994905	15.239253827319269
13	24.383702668612273	34.31170083842406	20.918402047832217	20.38619444513145
14	15.620285410432071	31.745727499814507	35.61941978087206	17.01456730888136
15	14.461578413672	28.754359063141493	30.467532708431232	26.316529814755274
16	16.852107189671802	24.922711646427423	36.43589394801276	21.789287215888013
17	16.401361511636534	26.64299582024584	28.355705735414144	28.599936932703486
18	19.272933000272054	27.14537011846759	26.734041500754337	26.847655380506026
19	20.342912967130808	30.473097469888454	29.511475774738454	19.672513788242277
20	24.96939381198526	32.43452130685331	22.741325155195014	19.854759725966414
21	19.27571538100067	29.49988252170257	25.401126555041674	25.823275542255086
22	19.188379541463657	32.32090742710162	24.948835109934954	23.541877921499765
23	22.01898820270571	28.397750599757625	20.187717953157076	29.395543244379592
24	16.9941631835382	35.32603319071056	25.990063809264708	21.689739816486533
25	19.40633269853832	25.024113966314644	28.095707713995992	27.47384562115104
26	22.16258996364356	32.35012242475206	21.79995300868103	23.687334602923354
27	17.430996957930404	28.094316523631687	29.197994212648084	25.276692305789826
28	20.515575149011948	26.13675710434546	31.39545668142359	21.952211065219004
29	19.367224791630598	29.17171617243341	31.673076447456282	19.787982588479707
30	26.556123710830263	24.26545148764623	24.510455568471297	24.667969233052208
31	26.60326960650953	27.17303934904657	25.779839487547303	20.443851556896593
32	29.656932456162043	23.629368337743923	21.326948284816858	25.386750921277173
33	26.410048722578093	22.90069374026167	26.137529987881187	24.551727549279054
34	18.945384957831475	24.05878243019316	27.94174931367942	29.054083298295946
35	23.94996042836297	25.76159943610417	29.256115054534664	21.03232508099819
36	23.016626270620534	22.680731085994115	34.09127445403507	20.211368189350285
37	27.91176143249326	27.31045804169861	20.691638018450277	24.086142507357852
38	24.492679247149606	25.776902530111546	26.643923280488707	23.08649494225014
39	23.767405337224545	23.137350678900898	26.77222194741919	26.323022036455367
40	22.838399327282172	28.21952365641926	20.533815200455074	28.408261815843495
41	18.974754532189053	20.144900205277867	30.939300818638245	29.94104444389484
42	24.946825612742067	26.817049192491282	22.112197957114237	26.12392723765241
43	23.192998293473153	21.31288180446665	28.407488932307768	27.086630969752427
44	22.02439838745579	21.93675339450449	26.344508198748546	29.69434001929117
45	27.700145920411543	26.99944970692256	23.576966834021714	21.723437538644177
46	19.446522642396065	22.685986694037048	35.19387010610145	22.673620557465437
47	23.35484010585413	25.427559171963495	23.64188905102268	27.5757116711597
48	20.868628348131477	19.415143570845597	35.080874533178346	24.63535354784458
49	24.090316078450773	19.096870130833725	30.589030000247323	26.22378379046818
50	20.729973041822277	22.176965597408056	24.884376623055424	32.20868473771424
51	17.88792570425148	22.492765810105606	32.83951227957362	26.7797962060693
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4104.0
1	3354.5
2	2605.0
3	1948.5
4	1292.0
5	1300.0
6	1308.0
7	1438.5
8	1569.0
9	1751.5
10	1934.0
11	1978.5
12	2023.0
13	2077.5
14	2132.0
15	2140.5
16	2149.0
17	2028.5
18	1908.0
19	1911.5
20	1915.0
21	1817.0
22	1719.0
23	1829.0
24	1939.0
25	2202.0
26	2539.5
27	2614.0
28	3135.0
29	3656.0
30	4538.0
31	5420.0
32	6497.5
33	7575.0
34	8173.0
35	8771.0
36	10080.5
37	11390.0
38	12509.0
39	13628.0
40	17614.0
41	21600.0
42	28716.0
43	35832.0
44	63708.5
45	91585.0
46	87901.5
47	84218.0
48	75069.5
49	65921.0
50	60472.0
51	55023.0
52	49619.0
53	44215.0
54	39072.5
55	33930.0
56	31213.5
57	28497.0
58	26438.0
59	24379.0
60	21269.0
61	18159.0
62	17743.5
63	17328.0
64	14928.5
65	12529.0
66	11326.0
67	10123.0
68	8660.5
69	7198.0
70	5877.0
71	4556.0
72	4083.5
73	3611.0
74	3039.5
75	2028.5
76	1589.0
77	1187.0
78	785.0
79	656.0
80	527.0
81	455.0
82	383.0
83	308.0
84	233.0
85	146.0
86	59.0
87	41.0
88	23.0
89	21.5
90	20.0
91	13.5
92	7.0
93	8.5
94	10.0
95	5.5
96	1.0
97	2.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	646928.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.084903928825156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.5879252328352	21.28546404897036
2	9.413743479659477	5.099406755143623
3	3.4307020489189046	2.787607062101764
4	1.6980411561643358	1.8396512632760893
5	0.92686014122415	1.2551958940256709
6	0.6011622039111554	0.9769452323144664
7	0.4451511131028139	0.8439812592539506
8	0.32802252162859624	0.7107566787841201
9	0.265787290945084	0.647894091667527
>10	2.89895266790634	19.008259603202944
>50	1.1275144418137355	21.83153722443621
>100	0.26063724876957023	9.73333002598259
>500	0.009185453701130228	1.5801092660037237
>1k	0.004018635994244475	2.769156260407431
>5k	5.740908563206393E-4	1.1077247009457318
>10k+	0.0017222725689619177	8.522980633483773
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCC	19808	3.0618554151312045	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGT	17913	2.7689325550911383	No Hit
AATCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCT	17092	2.6420250785249673	No Hit
CTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGCT	7124	1.101204461702075	No Hit
AATGACTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTT	3699	0.5717792397299235	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3425	0.5294252219721515	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCT	3098	0.47887863873568615	No Hit
AACTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTG	2508	0.3876783815200455	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTGCCATCGTAT	2200	0.34006875571933814	No Hit
AAACTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCT	1661	0.2567519105681003	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTT	1218	0.1882744293027972	No Hit
AATGCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTC	940	0.1453021047164445	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	802	0.12397051913041326	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTGCCATC	790	0.12211559864467143	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	776	0.11995152474463927	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.637301214354611E-4	0.0	0.0	1.4425098310785744	0.0
2	4.637301214354611E-4	0.0	0.0	1.6842678010535947	0.0
3	4.637301214354611E-4	0.0	0.0	2.4806469962654267	0.0
4	4.637301214354611E-4	0.0	0.0	6.9740372962679	0.0
5	4.637301214354611E-4	0.0	0.0	7.418136175895927	0.0
6	4.637301214354611E-4	0.0	0.0	9.430106596097247	0.0
7	4.637301214354611E-4	0.0	0.0	11.002306284470606	0.0
8	4.637301214354611E-4	0.0	0.0	11.779981698117874	0.0
9	4.637301214354611E-4	0.0	0.0	15.86250711052853	0.0
10	4.637301214354611E-4	0.0	0.0	17.259416812999284	0.0
11	4.637301214354611E-4	0.0	0.0	22.253017337323474	0.0
12	4.637301214354611E-4	0.0	0.0	23.14461578413672	0.0
13	4.637301214354611E-4	0.0	0.0	23.631068681522517	0.0
14	4.637301214354611E-4	0.0	0.0	24.376282986669306	0.0
15	6.183068285806148E-4	0.0	0.0	24.73335518017461	0.0
16	6.183068285806148E-4	0.0	0.0	25.25010511216086	0.0
17	6.183068285806148E-4	0.0	0.0	25.78215813815448	0.0
18	6.183068285806148E-4	0.0	0.0	26.666027749610468	0.0
19	6.183068285806148E-4	0.0	0.0	27.016916874829967	0.0
20	0.001082036950016076	0.0	0.0	27.33890015581332	0.0
21	0.001082036950016076	0.0	0.0	27.707874755768803	0.0
22	0.001082036950016076	0.0	0.0	28.047943511488143	0.0
23	0.001082036950016076	0.0	0.0	28.427738480943784	0.0
24	0.001082036950016076	0.0	0.0	28.675524942497464	0.0
25	0.001082036950016076	0.0	0.0	28.909554077115228	0.0
26	0.001082036950016076	0.0	0.0	29.12565231370415	0.0
27	0.001082036950016076	0.0	0.0	29.366482823436304	0.0
28	0.001082036950016076	0.0	0.0	29.602985185368386	0.0
29	0.001082036950016076	0.0	0.0	29.83577770632899	0.0
30	0.001082036950016076	0.0	0.0	30.206916380184502	0.0
31	0.001082036950016076	0.0	0.0	30.45671233893107	0.0
32	0.001082036950016076	0.0	0.0	30.695842504884624	0.0
33	0.001082036950016076	0.0	0.0	30.93141740657384	0.0
34	0.001082036950016076	0.0	0.0	31.169001805455938	0.0
35	0.001082036950016076	0.0	0.0	31.442138846981425	0.0
36	0.001082036950016076	0.0	0.0	31.676013404892043	0.0
37	0.001082036950016076	0.0	0.0	31.90355031780971	0.0
38	0.001082036950016076	0.0	0.0	32.130159770484504	0.0
39	0.001082036950016076	0.0	0.0	32.39494966982415	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAC	20	7.030998E-4	45.0	14
TCGTTAG	25	3.888908E-5	45.0	23
ACCGGAT	25	3.888908E-5	45.0	37
CGAACAG	20	7.030998E-4	45.0	1
CATATAG	25	3.888908E-5	45.0	1
CGTATAG	20	7.030998E-4	45.0	1
GTCGAGT	25	3.888908E-5	45.0	17
GTCGAAC	20	7.030998E-4	45.0	26
CCGATTC	25	3.888908E-5	45.0	18
CGTGATA	20	7.030998E-4	45.0	30
TAGAACG	25	3.888908E-5	45.0	1
TAGCGAG	20	7.030998E-4	45.0	1
GATCCGA	20	7.030998E-4	45.0	38
ACCGATA	20	7.030998E-4	45.0	11
ATTCGTA	25	3.888908E-5	45.0	31
GTGATAA	20	7.030998E-4	45.0	17
TTCCGCA	20	7.030998E-4	45.0	15
CGATTCG	20	7.030998E-4	45.0	1
GCTTGTA	20	7.030998E-4	45.0	27
TGTTGCG	20	7.030998E-4	45.0	13
>>END_MODULE