FastQCFastQC Report
Sat 18 Jun 2016
SRR3548837_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548837_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375233
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCC38621.0292271735161886No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGT35320.9412818168977676No Hit
CTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGCT32580.8682605207964118No Hit
AATCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCT32130.8562679721666272No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31020.8266863522131582No Hit
AAACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCT10180.27129810011379596No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTT7300.19454578888317392No Hit
AATGACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTT7270.1937462856411883No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6030.1607001516391149No Hit
CTTCGAGGGAGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTC6010.16016714947779115No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCT5490.14630909328337327No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG4960.13218453600829352No Hit
AACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTG4960.13218453600829352No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4820.12845352087902717No Hit
AATGCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTC4730.12605501115307022No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTAT4080.1087324409100479No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG453.8380676E-1045.0000042
CTAACGG453.8380676E-1045.0000042
TCTACAG453.8380676E-1045.0000041
CGGTAGT453.8380676E-1045.00000412
TCGACAA453.8380676E-1045.00000419
ATCGACA453.8380676E-1045.00000418
TGCAACG453.8380676E-1045.0000041
TAATCAC253.885766E-545.038
AACGTTG207.027207E-445.01
AAACTTG253.885766E-545.044
TTTAGCG207.027207E-445.041
ATCCTAC207.027207E-445.028
CGGGTAT750.045.06
CTAGAAC253.885766E-545.013
TCGTTTG207.027207E-445.041
TCGTTTA207.027207E-445.010
AGCCGAA207.027207E-445.025
GGTACGT253.885766E-545.08
GGTACCT253.885766E-545.08
AAATCGA253.885766E-545.028