##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548837_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	375233
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.302681267372538	31.0	31.0	34.0	30.0	34.0
2	31.499574930776344	31.0	31.0	34.0	30.0	34.0
3	31.469263097861862	31.0	31.0	34.0	30.0	34.0
4	35.447529934733886	37.0	35.0	37.0	33.0	37.0
5	35.127656682647846	37.0	35.0	37.0	32.0	37.0
6	35.182689155804525	37.0	35.0	37.0	32.0	37.0
7	35.06979663302535	37.0	35.0	37.0	32.0	37.0
8	35.423640777863355	37.0	35.0	37.0	33.0	37.0
9	35.895294390418755	39.0	37.0	39.0	32.0	39.0
10	35.80789802602649	38.0	35.0	39.0	30.0	39.0
11	36.17463549314692	38.0	35.0	39.0	32.0	39.0
12	35.615422950540065	37.0	35.0	39.0	31.0	39.0
13	35.2012802711915	37.0	34.0	39.0	30.0	39.0
14	36.12145786751165	38.0	35.0	40.0	30.0	41.0
15	36.38908891275554	38.0	35.0	40.0	31.0	41.0
16	36.47242646568932	38.0	35.0	40.0	31.0	41.0
17	36.48029091257965	38.0	35.0	40.0	31.0	41.0
18	36.278251113308265	38.0	35.0	40.0	31.0	41.0
19	36.27066382754182	38.0	35.0	40.0	31.0	41.0
20	36.27172983186447	38.0	35.0	40.0	31.0	41.0
21	36.27788867183857	38.0	35.0	40.0	31.0	41.0
22	36.24983143806648	38.0	35.0	40.0	31.0	41.0
23	36.250657591416534	38.0	35.0	40.0	31.0	41.0
24	35.849115083161664	37.0	34.0	40.0	30.0	41.0
25	35.9302646622232	37.0	34.0	40.0	30.0	41.0
26	35.83240013538255	37.0	34.0	40.0	30.0	41.0
27	35.708967494863195	37.0	34.0	40.0	30.0	41.0
28	35.58729909149782	37.0	34.0	40.0	29.0	41.0
29	35.42088782170012	37.0	34.0	40.0	29.0	41.0
30	35.41422795969438	37.0	34.0	40.0	29.0	41.0
31	35.24779536981023	37.0	34.0	40.0	29.0	41.0
32	35.140752545751575	37.0	34.0	40.0	28.0	41.0
33	35.01918541279685	37.0	34.0	40.0	27.0	41.0
34	35.087940026596804	37.0	34.0	40.0	28.0	41.0
35	34.84995989158736	36.0	34.0	40.0	27.0	41.0
36	34.73196387311351	36.0	34.0	40.0	26.0	41.0
37	34.52414100039176	36.0	33.0	40.0	25.0	41.0
38	34.496901924937305	36.0	33.0	40.0	26.0	41.0
39	34.46789594731807	36.0	33.0	40.0	25.0	41.0
40	34.2222592362612	36.0	33.0	40.0	24.0	41.0
41	34.365921973813606	36.0	33.0	40.0	25.0	41.0
42	34.369010721338476	36.0	33.0	40.0	25.0	41.0
43	34.26324976747781	35.0	33.0	40.0	25.0	41.0
44	34.23488072744135	35.0	33.0	39.0	25.0	41.0
45	34.016205930715046	35.0	33.0	39.0	24.0	41.0
46	33.87843553205608	35.0	33.0	39.0	23.0	41.0
47	33.7904262151783	35.0	33.0	39.0	23.0	40.0
48	33.77061185983109	35.0	33.0	39.0	23.0	40.0
49	33.650505152798395	35.0	33.0	39.0	23.0	40.0
50	33.4766052026341	35.0	33.0	39.0	23.0	40.0
51	31.633211897674244	34.0	30.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	8.0
11	6.0
12	10.0
13	16.0
14	14.0
15	25.0
16	69.0
17	150.0
18	339.0
19	641.0
20	1089.0
21	1575.0
22	2069.0
23	2775.0
24	3341.0
25	3839.0
26	4467.0
27	5349.0
28	6622.0
29	8191.0
30	10759.0
31	14201.0
32	18898.0
33	26992.0
34	45863.0
35	47049.0
36	35469.0
37	45430.0
38	53572.0
39	36399.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.09622288018378	35.749520964307514	20.023292194449848	26.13096396105886
2	22.529201855913524	36.58793336406979	20.443830899734298	20.439033880282384
3	20.814267401854313	31.005801728526013	22.49295770894351	25.686973160676168
4	21.84189556888653	33.107695751706274	18.36965298894287	26.680755690464323
5	17.259942489066795	37.47351645510923	17.27646555606783	27.990075499756152
6	18.959419880447616	37.480978485367764	21.87520820396927	21.68439343021536
7	77.87108276724062	10.15848819266962	6.460785698486007	5.50964334160375
8	80.20723124032268	6.974066779840793	4.974775672715353	7.843926307121175
9	74.36899206626283	11.807330378724686	8.653290089091312	5.170387465921174
10	48.24868814843044	25.908702059786854	11.92672286286121	13.915886928921498
11	40.30695594470636	24.521030932780434	19.052695258679275	16.11931786383394
12	37.3304053747938	23.928332529388406	24.473060738261292	14.268201357556503
13	24.27718244397481	37.528948679886895	20.11683407376217	18.077034802376126
14	15.754477884407821	40.232868644282355	28.002068048385937	16.01058542292389
15	14.821990603171894	27.64469009921835	37.282168679193994	20.251150618415757
16	15.71716773311516	24.64975095474012	39.13248568222944	20.50059562991528
17	16.42099708714319	24.79526054478151	26.093920310846862	32.68982205722844
18	21.933838441714883	26.284202082439446	27.32302329485946	24.458936180986214
19	24.267854906151644	30.70305650089411	25.26563495214973	19.763453640804514
20	28.605959497165763	27.979681957610335	22.18301695213374	21.23134159309016
21	21.430417900344587	30.41416932945663	23.87796382514331	24.277448945055472
22	19.67737379175073	30.873084190356394	24.077839635639723	25.371702382253158
23	21.111949108953638	30.213760516798892	20.277800726481946	28.39648964776552
24	18.987668994997776	30.78780384454459	28.561453816695227	21.66307334376241
25	18.00694501816205	26.234099879275007	30.20576548437904	25.553189618183904
26	20.70580146202493	33.79020501928135	22.839942115965282	22.66405140272844
27	17.630112490106146	33.422433527967954	26.38813750389758	22.559316478028318
28	18.34966540789323	30.04879634786919	31.989190716168352	19.612347528069225
29	20.52937774662676	29.744452113753322	28.872727078908305	20.85344306071161
30	21.474657079734456	30.93517894215061	25.27869350510216	22.31147047301277
31	27.45574083302908	31.772258836509582	19.32692487068035	21.44507545978099
32	29.238633062657073	29.238899563737732	20.969637531880192	20.55282984172501
33	25.522808494988446	29.431046842894947	23.703938619471103	21.342206042645504
34	22.58916459906245	28.75413409801377	22.30320893951225	26.353492363411533
35	21.40083628039112	30.31822894041835	25.887648474414565	22.393286304775966
36	28.021522627274255	27.7478260174345	25.17209307283741	19.058558282453834
37	22.15316883109961	34.08655422097737	22.169425397019985	21.59085155090304
38	23.181596501373814	31.705367065263452	21.88720075259905	23.225835680763684
39	24.331815165510495	26.8438543518294	24.416562509160975	24.407767973499134
40	24.364328297351246	27.271055584130394	21.65907582755248	26.705540290965878
41	19.19260832602676	23.368946761079116	28.38742861102302	29.0510163018711
42	26.16854061343219	26.665032126705274	21.499441680236014	25.666985579626527
43	22.916161425034577	26.11177588325121	25.734676854114642	25.237385837599575
44	21.462398030024012	27.031471112615364	25.50015590313219	26.00597495422844
45	22.54838993372118	31.686978490697783	22.82128704031895	22.943344535262092
46	20.904877769279352	28.175027249735496	28.527341678370504	22.392753302614643
47	21.55514040609435	26.82333376861843	24.890401430577803	26.731124394709422
48	23.115237732289003	22.184615958617712	31.42234291760053	23.277803391492753
49	22.40234734151847	21.676398397795506	29.63518667068195	26.286067590004077
50	20.672488826942192	26.60533588463701	26.329773767232627	26.39240152118817
51	18.66813419928418	27.487187960547182	28.57850988585759	25.266167954311054
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3511.0
1	2685.0
2	1859.0
3	1296.0
4	733.0
5	669.0
6	605.0
7	617.0
8	629.0
9	693.5
10	758.0
11	820.0
12	882.0
13	968.5
14	1055.0
15	1094.5
16	1134.0
17	1085.0
18	1036.0
19	1131.5
20	1227.0
21	1187.0
22	1147.0
23	1302.5
24	1458.0
25	1536.0
26	1677.5
27	1741.0
28	2301.0
29	2861.0
30	3287.0
31	3713.0
32	4470.0
33	5227.0
34	5416.0
35	5605.0
36	6317.5
37	7030.0
38	8470.0
39	9910.0
40	12170.5
41	14431.0
42	20086.0
43	25741.0
44	34713.0
45	43685.0
46	43358.5
47	43032.0
48	40317.0
49	37602.0
50	34368.5
51	31135.0
52	28797.0
53	26459.0
54	23460.5
55	20462.0
56	19078.5
57	17695.0
58	16415.0
59	15135.0
60	14492.5
61	13850.0
62	11908.0
63	9966.0
64	8892.5
65	7819.0
66	6195.0
67	4571.0
68	4008.5
69	3446.0
70	2809.5
71	2173.0
72	1817.0
73	1461.0
74	1392.5
75	976.0
76	628.0
77	441.0
78	254.0
79	283.0
80	312.0
81	243.5
82	175.0
83	123.5
84	72.0
85	52.0
86	32.0
87	30.0
88	28.0
89	16.0
90	4.0
91	3.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	375233.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.17282431894003
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.60627179787949	21.63127238249569
2	9.170179343099734	4.98359344526444
3	3.093756513153335	2.521983066524762
4	1.5130884677583172	1.6445954845364363
5	0.8264662088354255	1.122871054911271
6	0.48768326204404094	0.795103896168619
7	0.35377012952299264	0.6729053505171857
8	0.2678259671297027	0.5822070362292494
9	0.16789090442300847	0.4105863045570925
>10	2.6872538208928956	18.58527596890336
>50	1.4270726873304864	27.890812663307518
>100	0.3877480410953983	13.403253185558542
>500	0.004996753106899463	0.8716812416557178
>1k	0.005996103728279355	4.8838589193701205
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCC	3862	1.0292271735161886	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGT	3532	0.9412818168977676	No Hit
CTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGCT	3258	0.8682605207964118	No Hit
AATCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCT	3213	0.8562679721666272	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3102	0.8266863522131582	No Hit
AAACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCT	1018	0.27129810011379596	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTT	730	0.19454578888317392	No Hit
AATGACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTT	727	0.1937462856411883	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	603	0.1607001516391149	No Hit
CTTCGAGGGAGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTC	601	0.16016714947779115	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCT	549	0.14630909328337327	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	496	0.13218453600829352	No Hit
AACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTG	496	0.13218453600829352	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	482	0.12845352087902717	No Hit
AATGCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTC	473	0.12605501115307022	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGAAGCCATCGTAT	408	0.1087324409100479	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.665010806618821E-4	0.0	0.0	1.120637044183214	0.0
2	2.665010806618821E-4	0.0	0.0	1.340233934648605	0.0
3	2.665010806618821E-4	0.0	0.0	1.7303915167376003	0.0
4	2.665010806618821E-4	0.0	0.0	4.0814640503367245	0.0
5	2.665010806618821E-4	0.0	0.0	4.450834548134092	0.0
6	2.665010806618821E-4	0.0	0.0	5.8305106427206566	0.0
7	2.665010806618821E-4	0.0	0.0	6.857339306510888	0.0
8	2.665010806618821E-4	0.0	0.0	7.428984124530625	0.0
9	2.665010806618821E-4	0.0	0.0	9.32300730479462	0.0
10	2.665010806618821E-4	0.0	0.0	10.534254716402875	0.0
11	2.665010806618821E-4	0.0	0.0	13.010582757913083	0.0
12	2.665010806618821E-4	0.0	0.0	13.708549088166553	0.0
13	2.665010806618821E-4	0.0	0.0	14.074455071915317	0.0
14	2.665010806618821E-4	0.0	0.0	14.532836930653755	0.0
15	2.665010806618821E-4	0.0	0.0	14.79560699618637	0.0
16	2.665010806618821E-4	0.0	0.0	15.314751101315716	0.0
17	2.665010806618821E-4	0.0	0.0	15.890926437706705	0.0
18	2.665010806618821E-4	0.0	0.0	16.787702574133938	0.0
19	2.665010806618821E-4	0.0	0.0	17.120562423880628	0.0
20	2.665010806618821E-4	0.0	0.0	17.447026247691433	0.0
21	2.665010806618821E-4	0.0	0.0	17.788947134180628	0.0
22	2.665010806618821E-4	0.0	0.0	18.107149424490917	0.0
23	2.665010806618821E-4	0.0	0.0	18.444273291528198	0.0
24	2.665010806618821E-4	0.0	0.0	18.670532709010136	0.0
25	2.665010806618821E-4	0.0	0.0	18.872274027071178	0.0
26	2.665010806618821E-4	0.0	0.0	19.068685323518988	0.0
27	2.665010806618821E-4	0.0	0.0	19.249639557288404	0.0
28	2.665010806618821E-4	0.0	0.0	19.439121825639003	0.0
29	2.665010806618821E-4	0.0	0.0	19.642995152345343	0.0
30	2.665010806618821E-4	0.0	0.0	19.938278349718708	0.0
31	2.665010806618821E-4	0.0	0.0	20.1802613309597	0.0
32	2.665010806618821E-4	0.0	0.0	20.38013714145611	0.0
33	2.665010806618821E-4	0.0	0.0	20.59680252003422	0.0
34	2.665010806618821E-4	0.0	0.0	20.803074356466514	0.0
35	2.665010806618821E-4	0.0	0.0	21.067443428483102	0.0
36	2.665010806618821E-4	0.0	0.0	21.27264926059275	0.0
37	2.665010806618821E-4	0.0	0.0	21.472525071089162	0.0
38	2.665010806618821E-4	0.0	0.0	21.663872847004395	0.0
39	2.665010806618821E-4	0.0	0.0	21.898926800148175	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCGG	45	3.8380676E-10	45.000004	2
CTAACGG	45	3.8380676E-10	45.000004	2
TCTACAG	45	3.8380676E-10	45.000004	1
CGGTAGT	45	3.8380676E-10	45.000004	12
TCGACAA	45	3.8380676E-10	45.000004	19
ATCGACA	45	3.8380676E-10	45.000004	18
TGCAACG	45	3.8380676E-10	45.000004	1
TAATCAC	25	3.885766E-5	45.0	38
AACGTTG	20	7.027207E-4	45.0	1
AAACTTG	25	3.885766E-5	45.0	44
TTTAGCG	20	7.027207E-4	45.0	41
ATCCTAC	20	7.027207E-4	45.0	28
CGGGTAT	75	0.0	45.0	6
CTAGAAC	25	3.885766E-5	45.0	13
TCGTTTG	20	7.027207E-4	45.0	41
TCGTTTA	20	7.027207E-4	45.0	10
AGCCGAA	20	7.027207E-4	45.0	25
GGTACGT	25	3.885766E-5	45.0	8
GGTACCT	25	3.885766E-5	45.0	8
AAATCGA	25	3.885766E-5	45.0	28
>>END_MODULE