FastQCFastQC Report
Sat 18 Jun 2016
SRR3548835_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548835_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461374
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCC44080.9554071100668872No Hit
AATCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCT39760.8617737453779364No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGT39510.8563551478843628No Hit
CTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGCT31250.6773246866966929Illumina Single End Adapter 1 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25910.5615834442339621No Hit
AAACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCT10540.22844807032906062No Hit
AATGACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTT8620.18683324157841577No Hit
AATGATCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCT7780.16862675400000868No Hit
AACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTG6820.14781933962468627No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTT6080.1317802910437086No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA5920.1283123886478215No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5730.12419425455270561No Hit
GTATTGGGATAAAGCAAATATGAGTAAGTAAGTAAGTAAGTAAGTAAGTAA5240.11357380346530146No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4870.10555427917481262No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTAT4710.10208637677892557No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGAA302.1626656E-645.00000425
CAGCACG600.045.0000041
ATTACCG302.1626656E-645.0000041
ATTAACG302.1626656E-645.0000041
ATAGACG551.8189894E-1245.0000041
ATCCCCG302.1626656E-645.00000438
CGCACCG302.1626656E-645.0000041
TACGTGC302.1626656E-645.00000427
CTTCGTC207.0288917E-445.038
CTTCGCA207.0288917E-445.010
CCCCTAC406.7993824E-945.031
AGATCTA207.0288917E-445.035
AGATCGA207.0288917E-445.045
GTCGACA253.8871636E-545.014
GATCGCG253.8871636E-545.01
CCTAGCG207.0288917E-445.01
TCTTCGT207.0288917E-445.037
CCGGCTA207.0288917E-445.02
TTTCGCG253.8871636E-545.01
CGTTGCG253.8871636E-545.01