##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548835_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	461374
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.218772622644536	31.0	31.0	34.0	28.0	34.0
2	31.421798800972745	31.0	31.0	34.0	30.0	34.0
3	31.369218898334108	31.0	31.0	34.0	28.0	34.0
4	35.38439747363311	37.0	35.0	37.0	33.0	37.0
5	35.06124749118936	37.0	35.0	37.0	32.0	37.0
6	35.11788917450918	37.0	35.0	37.0	32.0	37.0
7	35.05009385010859	37.0	35.0	37.0	32.0	37.0
8	35.422438195476985	37.0	35.0	37.0	33.0	37.0
9	35.92954089307156	39.0	37.0	39.0	32.0	39.0
10	35.743977770745644	38.0	35.0	39.0	30.0	39.0
11	36.1689735442396	38.0	35.0	39.0	32.0	39.0
12	35.854887791683105	37.0	35.0	39.0	31.0	39.0
13	35.60563447441772	37.0	35.0	39.0	30.0	39.0
14	36.62492468149484	38.0	35.0	40.0	31.0	41.0
15	36.72628063133163	38.0	35.0	40.0	31.0	41.0
16	36.724286587453996	38.0	35.0	40.0	31.0	41.0
17	36.709521993003506	38.0	35.0	40.0	31.0	41.0
18	36.53874513951805	38.0	35.0	40.0	31.0	41.0
19	36.557413291602906	38.0	35.0	40.0	31.0	41.0
20	36.55152869472489	38.0	35.0	40.0	31.0	41.0
21	36.580784786312186	38.0	35.0	40.0	31.0	41.0
22	36.58557265905751	38.0	35.0	40.0	31.0	41.0
23	36.534414596401184	38.0	35.0	40.0	31.0	41.0
24	36.15229943603237	38.0	35.0	40.0	30.0	41.0
25	36.291600740397165	38.0	35.0	40.0	30.0	41.0
26	36.137307260487155	38.0	35.0	40.0	30.0	41.0
27	36.04516509382844	38.0	34.0	40.0	30.0	41.0
28	35.9462019966448	38.0	34.0	40.0	30.0	41.0
29	35.6783325458305	38.0	34.0	40.0	29.0	41.0
30	35.76872558921829	38.0	34.0	40.0	29.0	41.0
31	35.58831880426725	38.0	34.0	40.0	29.0	41.0
32	35.537767624530204	38.0	34.0	40.0	29.0	41.0
33	35.39610164421922	38.0	34.0	40.0	28.0	41.0
34	35.3114674862476	38.0	34.0	40.0	28.0	41.0
35	35.26461612487917	38.0	34.0	40.0	27.0	41.0
36	34.89577436093061	37.0	33.0	40.0	26.0	41.0
37	34.90646200262694	37.0	33.0	40.0	26.0	41.0
38	34.75677433058647	37.0	33.0	40.0	26.0	41.0
39	34.62452804015831	37.0	33.0	40.0	25.0	41.0
40	34.5516890852107	37.0	33.0	40.0	25.0	41.0
41	34.54294130141707	37.0	33.0	40.0	24.0	41.0
42	34.62471877479008	37.0	33.0	40.0	26.0	41.0
43	34.446739521516164	37.0	33.0	40.0	24.0	41.0
44	34.46687502980229	37.0	33.0	40.0	25.0	41.0
45	34.26241183941878	36.0	33.0	40.0	24.0	41.0
46	34.05655932063792	36.0	33.0	40.0	23.0	41.0
47	34.01206613289869	36.0	33.0	39.0	23.0	41.0
48	33.963688894476064	36.0	33.0	39.0	23.0	41.0
49	33.80637833948163	36.0	33.0	39.0	23.0	41.0
50	33.658970813266464	35.0	32.0	39.0	23.0	40.0
51	31.91853463784262	35.0	30.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	7.0
10	12.0
11	12.0
12	15.0
13	18.0
14	27.0
15	44.0
16	111.0
17	169.0
18	388.0
19	663.0
20	1144.0
21	1606.0
22	2307.0
23	3065.0
24	3747.0
25	4405.0
26	5434.0
27	6520.0
28	8379.0
29	10661.0
30	13204.0
31	17246.0
32	22297.0
33	30321.0
34	47817.0
35	50626.0
36	46791.0
37	59996.0
38	72873.0
39	51468.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.525864049556326	34.83529631058534	19.836835192273515	24.80200444758482
2	22.745104839024304	34.94431849215604	19.69465119404214	22.615925474777512
3	20.513943135070463	30.612041424094116	22.997177994425346	25.87683744641007
4	22.93779016589578	32.25691087924331	18.60529635393412	26.200002600926798
5	17.415372344345368	36.26363860989132	18.1973843346179	28.123604711145404
6	21.244803565003664	36.3720105597628	20.808498094821122	21.57468778041242
7	78.89304555523285	9.643803075162449	6.386575749825521	5.076575619779181
8	81.43263382852089	5.9964367302882255	4.856363817640352	7.714565623550524
9	74.52348853641514	11.72454451269469	8.423534919609688	5.32843203128048
10	41.13604147611266	31.342468366227834	12.486399320291131	15.035090837368381
11	33.8215850914876	24.821294654661944	22.952095263278814	18.40502499057164
12	31.18034392922011	24.543645719091238	28.247365477898622	16.028644873790025
13	23.248384174227414	34.34545509716629	22.912214385726113	19.493946342880182
14	17.699306853008622	34.77569173815603	29.779311361281735	17.745690047553612
15	16.20724184717821	28.980610090728998	32.92144767585516	21.89070038623763
16	17.431628136826088	26.469198524407528	34.068022905495326	22.031150433271055
17	17.361836601108863	26.010785176451208	28.189928344466747	28.437449877973187
18	22.380324855756932	26.296020148512923	27.055274029312443	24.2683809664177
19	22.564990658337923	30.519708522803622	26.728424228500085	20.186876590358363
20	26.248336490569475	29.439240182585063	23.932427921816142	20.379995405029323
21	21.419715892096217	29.060155101934654	25.06491479797301	24.455214207996114
22	21.270812832972815	27.98163745681378	24.596964718427998	26.150584991785408
23	22.05976062803712	29.64709758243854	22.13670471244587	26.156437077078465
24	18.260240065543357	30.920467993428325	28.660262606908926	22.15902933411939
25	21.005735043587197	27.300628123821458	26.243351380875385	25.45028545171596
26	22.24204224772094	32.3628986462176	23.40053839184696	21.9945207142145
27	19.070212018882728	29.543927486160904	26.85673661714791	24.52912387780846
28	21.51638367138157	28.9145032013074	28.85424839717886	20.71486473013217
29	18.625670280509954	29.628674350960395	29.934066505698198	21.811588862831456
30	23.345702185211998	28.52414743787036	24.805472349980707	23.324678026936933
31	27.479008353309897	30.82488393364169	20.568345853905942	21.127761859142474
32	25.060146432178666	28.86053397027141	23.346352416911227	22.7329671806387
33	25.2662698808342	28.965871505546474	23.25748741801662	22.510371195602698
34	22.276504527780066	28.68540489927911	25.330209331258374	23.70788124168245
35	23.66778362023001	28.12382145504515	25.884640226800816	22.323754697924027
36	26.302305721605467	28.092176845682676	26.550694230710874	19.05482320200098
37	22.450983367073132	31.495272815546606	22.13692145634561	23.916822361034647
38	23.16927265082124	32.264280171834564	22.697854668880343	21.86859250846385
39	23.722186339065487	28.902148799022054	25.036304603206943	22.33936025870552
40	25.755677606453766	28.393450866325367	22.0036239580037	23.847247569217163
41	23.01581796980324	23.727171448759574	28.36809183005544	24.88891875138174
42	28.335796988993746	25.09135755374165	22.11546381027106	24.457381646993547
43	23.379297489672155	25.271471734428037	26.331782891970505	25.017447883929307
44	22.263716637695232	26.00883448135352	24.539094097196635	27.18835478375461
45	24.721809204679936	29.823743860729042	23.903167495350843	21.551279439240183
46	21.478670232826296	26.22362768599878	29.72425841074703	22.573443670427896
47	22.432560135594983	28.4107903783048	25.118667285109257	24.037982200990953
48	23.643941791258285	23.34765288030968	30.615509326490002	22.392896001942024
49	22.820314972235106	23.24188185723513	29.07532717491666	24.862475995613103
50	21.047349872337843	27.460151634032258	25.41690689115555	26.075591602474347
51	20.59478860967458	26.34023590406048	28.417726183096576	24.647249303168362
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2999.0
1	2355.5
2	1712.0
3	1183.0
4	654.0
5	595.5
6	537.0
7	607.0
8	677.0
9	693.0
10	709.0
11	780.0
12	851.0
13	885.5
14	920.0
15	932.5
16	945.0
17	996.0
18	1047.0
19	1133.5
20	1220.0
21	1157.0
22	1094.0
23	1279.0
24	1464.0
25	1682.0
26	3095.5
27	4291.0
28	4230.0
29	4169.0
30	5014.0
31	5859.0
32	6475.5
33	7092.0
34	8704.5
35	10317.0
36	10861.5
37	11406.0
38	12418.5
39	13431.0
40	16085.5
41	18740.0
42	24277.5
43	29815.0
44	38270.0
45	46725.0
46	47074.5
47	47424.0
48	46702.5
49	45981.0
50	43418.0
51	40855.0
52	38014.0
53	35173.0
54	31313.0
55	27453.0
56	24320.5
57	21188.0
58	19777.0
59	18366.0
60	17416.5
61	16467.0
62	14662.5
63	12858.0
64	10900.5
65	8943.0
66	7635.5
67	6328.0
68	5438.5
69	4549.0
70	3703.0
71	2857.0
72	2260.5
73	1664.0
74	1493.0
75	974.0
76	626.0
77	507.0
78	388.0
79	300.0
80	212.0
81	144.5
82	77.0
83	52.5
84	28.0
85	24.5
86	21.0
87	14.0
88	7.0
89	7.5
90	8.0
91	6.5
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	461374.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.343383017918374
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.07146833409857	23.495677641345768
2	8.652856088172884	5.078081407883675
3	2.840056930453413	2.5001063490896422
4	1.3883991360845587	1.6296131052750469
5	0.7631135707473823	1.1196166896305895
6	0.48003915266033126	0.8451583632065454
7	0.3509139882999996	0.720790249552255
8	0.2834874284466472	0.6654784154939758
9	0.20945665676274133	0.5531550214537604
>10	3.5095963222836617	25.650942186344444
>50	1.2729863848073963	25.79122832924962
>100	0.16800459846004592	6.797943980348197
>500	0.0051807585428208	1.0031213099874874
>1k	0.004440650179560685	4.149086951138979
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCC	4408	0.9554071100668872	No Hit
AATCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCT	3976	0.8617737453779364	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGT	3951	0.8563551478843628	No Hit
CTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTGCT	3125	0.6773246866966929	Illumina Single End Adapter 1 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2591	0.5615834442339621	No Hit
AAACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCT	1054	0.22844807032906062	No Hit
AATGACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTT	862	0.18683324157841577	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCT	778	0.16862675400000868	No Hit
AACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTTCTG	682	0.14781933962468627	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTATGCCGTCTT	608	0.1317802910437086	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	592	0.1283123886478215	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	573	0.12419425455270561	No Hit
GTATTGGGATAAAGCAAATATGAGTAAGTAAGTAAGTAAGTAAGTAAGTAA	524	0.11357380346530146	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	487	0.10555427917481262	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCAAAGGTCGTAT	471	0.10208637677892557	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9528061832699719	0.0
2	0.0	0.0	0.0	1.14939290033682	0.0
3	0.0	0.0	0.0	1.5757281511311865	0.0
4	0.0	0.0	0.0	3.9005232197739796	0.0
5	0.0	0.0	0.0	4.220437215794561	0.0
6	0.0	0.0	0.0	5.520250382552983	0.0
7	0.0	0.0	0.0	6.565389467113448	0.0
8	0.0	0.0	0.0	7.1085496798692605	0.0
9	0.0	0.0	0.0	8.932883084005601	0.0
10	0.0	0.0	0.0	10.143831251869416	0.0
11	0.0	0.0	0.0	12.591520111666457	0.0
12	0.0	0.0	0.0	13.276864322653639	0.0
13	0.0	0.0	0.0	13.632974549931292	0.0
14	0.0	0.0	0.0	14.051073532535426	0.0
15	0.0	0.0	0.0	14.336525248496882	0.0
16	0.0	0.0	0.0	14.852375729885082	0.0
17	0.0	0.0	0.0	15.420244747211589	0.0
18	0.0	0.0	0.0	16.27204827320135	0.0
19	0.0	0.0	0.0	16.59846458621422	0.0
20	0.0	0.0	0.0	16.934634374715525	0.0
21	0.0	0.0	0.0	17.287493443497034	0.0
22	0.0	0.0	0.0	17.643603670774684	0.0
23	0.0	0.0	0.0	18.053249641288847	0.0
24	0.0	0.0	0.0	18.31572650387755	0.0
25	0.0	0.0	0.0	18.535938306016376	0.0
26	0.0	0.0	0.0	18.742061754671916	0.0
27	0.0	0.0	0.0	18.962490300710485	0.0
28	0.0	0.0	0.0	19.20307602942515	0.0
29	0.0	0.0	0.0	19.43000689245601	0.0
30	0.0	0.0	0.0	19.80323988781336	0.0
31	0.0	0.0	0.0	20.03623958003702	0.0
32	0.0	0.0	0.0	20.25363371147919	0.0
33	0.0	0.0	0.0	20.48338224520671	0.0
34	0.0	0.0	0.0	20.717465656929086	0.0
35	0.0	0.0	0.0	21.01700572637383	0.0
36	0.0	0.0	0.0	21.223129175029367	0.0
37	0.0	0.0	0.0	21.462847928145063	0.0
38	0.0	0.0	0.0	21.688478327777464	0.0
39	0.0	0.0	0.0	21.93556637348442	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCGAA	30	2.1626656E-6	45.000004	25
CAGCACG	60	0.0	45.000004	1
ATTACCG	30	2.1626656E-6	45.000004	1
ATTAACG	30	2.1626656E-6	45.000004	1
ATAGACG	55	1.8189894E-12	45.000004	1
ATCCCCG	30	2.1626656E-6	45.000004	38
CGCACCG	30	2.1626656E-6	45.000004	1
TACGTGC	30	2.1626656E-6	45.000004	27
CTTCGTC	20	7.0288917E-4	45.0	38
CTTCGCA	20	7.0288917E-4	45.0	10
CCCCTAC	40	6.7993824E-9	45.0	31
AGATCTA	20	7.0288917E-4	45.0	35
AGATCGA	20	7.0288917E-4	45.0	45
GTCGACA	25	3.8871636E-5	45.0	14
GATCGCG	25	3.8871636E-5	45.0	1
CCTAGCG	20	7.0288917E-4	45.0	1
TCTTCGT	20	7.0288917E-4	45.0	37
CCGGCTA	20	7.0288917E-4	45.0	2
TTTCGCG	25	3.8871636E-5	45.0	1
CGTTGCG	25	3.8871636E-5	45.0	1
>>END_MODULE