SRR3548833_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548833_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	620531
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGCT	5034	0.8112406954688807	Illumina Single End Adapter 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2742	0.44187961600629133	No Hit
AATCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCT	1764	0.2842726632513122	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCC	1758	0.28330574943072945	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGT	1559	0.2512364410480701	No Hit
AAACTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCT	1404	0.22625783401635052	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTT	998	0.1608299988235882	No Hit
TAATCTGGGATGAAAGTGTAGAAACACACAACAAAGTAAATTATTAAGAAA	707	0.1139346785253275	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	658	0.10603821565723551	No Hit
CCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC	643	0.10362093110577876	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGA	20	7.030774E-4	45.0	3
TCACGAG	25	3.8887243E-5	45.0	1
TCGCCGG	20	7.030774E-4	45.0	2
TTGATCG	20	7.030774E-4	45.0	20
CGGAATC	40	6.8066583E-9	45.0	6
CTATGCG	35	1.2107193E-7	45.0	1
TCGTTGC	20	7.030774E-4	45.0	38
TCCGCGG	90	0.0	45.0	2
CTCCGCC	45	3.8380676E-10	45.0	45
CAACGGA	45	3.8380676E-10	45.0	3
CGTGCTA	25	3.8887243E-5	45.0	17
CACGGAA	20	7.030774E-4	45.0	4
CGAAAAG	20	7.030774E-4	45.0	1
GCGAATG	40	6.8066583E-9	45.0	1
TTAGCGT	20	7.030774E-4	45.0	30
GAGTCGT	20	7.030774E-4	45.0	35
CCGGCAT	25	3.8887243E-5	45.0	19
GACGTGC	20	7.030774E-4	45.0	9
CGCGACG	20	7.030774E-4	45.0	1
TGACGGT	20	7.030774E-4	45.0	27