##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548833_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	620531
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.097377890870884	31.0	31.0	34.0	28.0	34.0
2	31.31153479842264	31.0	31.0	34.0	28.0	34.0
3	31.30986042599	31.0	31.0	34.0	28.0	34.0
4	35.34937013622204	37.0	35.0	37.0	33.0	37.0
5	35.01363348487022	37.0	35.0	37.0	32.0	37.0
6	35.07873740393308	36.0	35.0	37.0	32.0	37.0
7	35.176437599410825	37.0	35.0	37.0	32.0	37.0
8	35.5140178331139	37.0	35.0	37.0	33.0	37.0
9	35.91432660092727	39.0	37.0	39.0	32.0	39.0
10	35.538382449869545	37.0	35.0	39.0	30.0	39.0
11	36.04496955027226	37.0	35.0	39.0	31.0	39.0
12	36.0170386330417	37.0	35.0	39.0	32.0	39.0
13	35.79534785530457	37.0	35.0	39.0	30.0	39.0
14	36.816160675292615	38.0	35.0	40.0	31.0	41.0
15	36.83676722033226	38.0	35.0	40.0	31.0	41.0
16	36.8721192011358	38.0	35.0	40.0	32.0	41.0
17	36.8789214398636	38.0	35.0	40.0	32.0	41.0
18	36.76153165595272	38.0	35.0	40.0	31.0	41.0
19	36.80044187961601	38.0	35.0	40.0	31.0	41.0
20	36.735508781994774	38.0	35.0	40.0	31.0	41.0
21	36.717870662384314	38.0	35.0	40.0	31.0	41.0
22	36.746784608665806	38.0	35.0	40.0	31.0	41.0
23	36.681194009646575	38.0	35.0	40.0	31.0	41.0
24	36.343647617927225	38.0	35.0	40.0	30.0	41.0
25	36.50975857773423	38.0	35.0	40.0	31.0	41.0
26	36.32686682857101	38.0	35.0	40.0	30.0	41.0
27	36.24033609924403	38.0	35.0	40.0	30.0	41.0
28	36.27397986563121	38.0	35.0	40.0	30.0	41.0
29	35.95295964262865	38.0	35.0	40.0	30.0	41.0
30	35.96184719216284	38.0	35.0	40.0	30.0	41.0
31	35.873922495411186	38.0	34.0	40.0	30.0	41.0
32	35.75757214385744	38.0	34.0	40.0	29.0	41.0
33	35.72564142645573	38.0	34.0	40.0	29.0	41.0
34	35.725198257621294	38.0	34.0	40.0	29.0	41.0
35	35.51523614452783	38.0	34.0	40.0	28.0	41.0
36	35.378738531999204	38.0	34.0	40.0	27.0	41.0
37	35.25607101015098	38.0	34.0	40.0	27.0	41.0
38	35.33419281228496	38.0	34.0	40.0	27.0	41.0
39	35.260546209617246	38.0	34.0	40.0	27.0	41.0
40	35.095208780866706	38.0	34.0	40.0	26.0	41.0
41	35.11275665518725	38.0	34.0	40.0	26.0	41.0
42	34.97575624747192	38.0	34.0	40.0	26.0	41.0
43	34.935627712394705	37.0	33.0	40.0	26.0	41.0
44	34.929499090295245	37.0	34.0	40.0	26.0	41.0
45	34.72898050218281	37.0	33.0	40.0	26.0	41.0
46	34.58562424762018	37.0	33.0	40.0	25.0	41.0
47	34.466087915027614	37.0	33.0	40.0	24.0	41.0
48	34.3354111236989	37.0	33.0	40.0	24.0	41.0
49	34.18072747372815	36.0	33.0	40.0	24.0	41.0
50	34.07256688223473	36.0	33.0	40.0	24.0	41.0
51	32.275639734356545	35.0	30.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	1.0
11	9.0
12	2.0
13	8.0
14	15.0
15	30.0
16	82.0
17	175.0
18	403.0
19	792.0
20	1225.0
21	1989.0
22	2679.0
23	3645.0
24	4539.0
25	5379.0
26	6603.0
27	8332.0
28	10407.0
29	13170.0
30	16910.0
31	21791.0
32	27918.0
33	37667.0
34	57958.0
35	65536.0
36	66689.0
37	86621.0
38	107145.0
39	72803.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.9226146638927	32.53004281816702	21.297243812154427	26.250098705785852
2	21.20409778077163	32.42545497324066	21.877553256807474	24.492893989180235
3	20.708554447723	32.473800664269795	21.71752901950104	25.10011586850617
4	19.145699409054505	32.57548776773441	20.830224436812987	27.4485883863981
5	17.782512074336335	35.95388465685034	18.404237660971006	27.859365607842317
6	20.32146661488306	36.5893081892766	20.657952624445837	22.4312725713945
7	82.8902987924858	6.1353904962040575	6.152956097277976	4.821354614032176
8	85.10968831532993	5.06340537378471	4.516293303638336	5.310613007247019
9	76.17701613618014	11.617953011211366	8.39329541956808	3.811735433040412
10	35.57404867766478	39.998968625258044	11.804728530887257	12.622254166189926
11	26.062033967682517	24.33335320878409	32.511510303272516	17.09310252026087
12	27.789747812760364	24.27501607494227	31.052920804923524	16.882315307373847
13	22.867834161387584	32.174701989102886	27.42731628234528	17.53014756716425
14	17.32886834017962	33.61943238935686	30.952522919886356	18.099176350577167
15	15.307051541341208	30.639887451231285	35.10976889148165	18.94329211594586
16	17.21767325081261	28.171517619587092	33.495828572625705	21.11498055697459
17	17.609756805058893	27.242796894917415	30.17270692358641	24.974739376437277
18	19.33054110108923	27.35447544119472	30.195590550673536	23.119392907042517
19	21.33672612649489	31.659981531946023	28.828374408369605	18.174917933189477
20	23.89260165890181	30.1826983663991	26.67715230987654	19.247547664822548
21	20.188193659946077	31.69607964791445	27.142399009880247	20.973327682259228
22	19.47106590968058	28.554093187930977	27.98715938446266	23.987681517925775
23	19.461557923778184	30.69935265119712	25.35280268028511	24.486286744739587
24	16.61770322514105	31.038094793007925	30.13580304610084	22.208398935750186
25	19.143604429109907	29.121993905219885	28.63708662419766	23.097315041472548
26	20.021239873592133	34.571520198023954	25.103016609967916	20.304223318416
27	16.93033869379612	32.77757275623619	26.949338550370573	23.34274999959712
28	20.449099239199974	28.745380972102925	29.390957099645306	21.4145626890518
29	18.965692286122692	30.557055167268032	28.9685769123541	21.508675634255177
30	22.283334756845345	31.074998670493496	25.842061073499956	20.799605499161203
31	24.46984920978968	30.396225168444445	22.768403190171	22.365522431594876
32	24.02845305069368	29.59513706809168	26.39030120977034	19.986108671444295
33	25.26239623806063	28.478351605318668	23.953678381901952	22.30557377471875
34	21.761684750640985	28.668189018759737	26.6255835727788	22.94454265782048
35	22.720863260659016	27.76589727185266	28.680436593820453	20.832802873667873
36	25.399375695976513	28.38069330943982	26.975928680436596	19.244002314147078
37	20.492610361126196	30.578649575927713	26.948210484246555	21.980529578699535
38	22.183420328718466	28.28448538429184	29.032393224512553	20.499701062477136
39	22.504113412545063	26.96013575470041	28.970833044602124	21.564917788152403
40	25.990643497262827	26.184832022896522	25.451911346894835	22.372613132945816
41	22.870090293635613	23.624927682903834	30.378659567370526	23.126322456090026
42	23.595597963679495	27.30838588241361	26.832180825776636	22.263835328130263
43	21.291925786141224	26.72324186865765	27.855336800256552	24.12949554494457
44	21.427293721022803	26.832503130383493	26.71276696893467	25.027436179659034
45	23.015127366723014	28.325418069363174	26.76626953367358	21.893185030240232
46	20.266997136323567	27.435212745213374	30.787180656566715	21.510609461896344
47	21.945076071944836	26.577560186356525	27.109523939980434	24.367839801718205
48	22.487192420684863	23.853602801471645	32.72116945003554	20.93803532780796
49	21.128678502766178	23.5077699583099	31.299967286082403	24.06358425284152
50	20.496478016408528	25.525719101865985	26.912434672885	27.065368208840496
51	20.22477523282479	24.772976692542358	30.95574596595496	24.04650210867789
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3219.0
1	2562.0
2	1905.0
3	1349.5
4	794.0
5	751.0
6	708.0
7	770.5
8	833.0
9	932.5
10	1032.0
11	1222.0
12	1412.0
13	1435.0
14	1458.0
15	1478.0
16	1498.0
17	1584.0
18	1670.0
19	1781.0
20	1892.0
21	1949.0
22	2006.0
23	2773.5
24	3541.0
25	3706.0
26	4940.5
27	6010.0
28	7417.5
29	8825.0
30	9755.0
31	10685.0
32	12446.5
33	14208.0
34	15645.0
35	17082.0
36	19694.5
37	22307.0
38	23611.0
39	24915.0
40	29363.0
41	33811.0
42	40124.0
43	46437.0
44	52928.5
45	59420.0
46	63226.5
47	67033.0
48	69552.5
49	72072.0
50	65670.0
51	59268.0
52	52663.0
53	46058.0
54	38988.5
55	31919.0
56	26919.0
57	21919.0
58	19508.0
59	17097.0
60	15130.0
61	13163.0
62	11026.5
63	8890.0
64	7190.5
65	5491.0
66	4413.5
67	3336.0
68	2538.5
69	1741.0
70	1329.0
71	917.0
72	791.0
73	665.0
74	673.0
75	548.5
76	416.0
77	266.0
78	116.0
79	91.5
80	67.0
81	78.5
82	90.0
83	56.0
84	22.0
85	15.0
86	8.0
87	8.5
88	9.0
89	7.0
90	5.0
91	3.0
92	1.0
93	2.0
94	3.0
95	4.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	620531.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.332366781299214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.61009949162411	25.86928302187938
2	9.937100511339525	6.6245415797321
3	3.500514406056	3.500412903176411
4	1.7192851676009935	2.292313752324952
5	0.9603990909086458	1.6006187377296657
6	0.6428949329458196	1.2857525824073295
7	0.46108818654792594	1.0758412386777707
8	0.3669149953695865	0.9784116162574208
9	0.30606533505903866	0.9181693806506201
>10	3.5328591514058587	27.47022228422791
>50	0.8246851537013443	18.986105003272563
>100	0.13227911102253095	6.436750702225509
>500	0.002907233209286395	0.6583077197338079
>1k	0.0024226943410719957	1.4902368326751336
>5k	4.845388682143992E-4	0.8130326450294378
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGCT	5034	0.8112406954688807	Illumina Single End Adapter 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2742	0.44187961600629133	No Hit
AATCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCT	1764	0.2842726632513122	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCC	1758	0.28330574943072945	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGT	1559	0.2512364410480701	No Hit
AAACTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCT	1404	0.22625783401635052	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTT	998	0.1608299988235882	No Hit
TAATCTGGGATGAAAGTGTAGAAACACACAACAAAGTAAATTATTAAGAAA	707	0.1139346785253275	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	658	0.10603821565723551	No Hit
CCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC	643	0.10362093110577876	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1132401120975424	0.0
2	0.0	0.0	0.0	1.4442469433436846	0.0
3	0.0	0.0	0.0	1.8600198861942434	0.0
4	0.0	0.0	0.0	4.0731244692046005	0.0
5	0.0	0.0	0.0	4.401391711292425	0.0
6	0.0	0.0	0.0	6.067706528763269	0.0
7	0.0	0.0	0.0	7.410588673249201	0.0
8	0.0	0.0	0.0	8.262762053789416	0.0
9	0.0	0.0	0.0	9.785329016600299	0.0
10	0.0	0.0	0.0	11.714483240966205	0.0
11	0.0	0.0	0.0	13.834441792593763	0.0
12	0.0	0.0	0.0	14.860659660838863	0.0
13	0.0	0.0	0.0	15.25806124109835	0.0
14	0.0	0.0	0.0	15.71025460452419	0.0
15	0.0	0.0	0.0	16.04400102492865	0.0
16	0.0	0.0	0.0	16.71117156113071	0.0
17	0.0	0.0	0.0	17.654395993109127	0.0
18	0.0	0.0	0.0	18.693828350235524	0.0
19	0.0	0.0	0.0	19.22434173312856	0.0
20	0.0	0.0	0.0	19.70393098813758	0.0
21	0.0	0.0	0.0	20.280050472901433	0.0
22	0.0	0.0	0.0	20.915151700720834	0.0
23	0.0	0.0	0.0	21.54928601471965	0.0
24	0.0	0.0	0.0	21.991971392243094	0.0
25	0.0	0.0	0.0	22.367295106932612	0.0
26	0.0	0.0	0.0	22.723441697513902	0.0
27	0.0	0.0	0.0	23.09119125394219	0.0
28	0.0	0.0	0.0	23.47682871605125	0.0
29	0.0	0.0	0.0	23.898080837218448	0.0
30	0.0	0.0	0.0	24.400392567011156	0.0
31	0.0	0.0	0.0	24.82551234346068	0.0
32	0.0	0.0	0.0	25.174890537297895	0.0
33	0.0	0.0	0.0	25.58292816958379	0.0
34	0.0	0.0	0.0	25.953417315170395	0.0
35	0.0	0.0	0.0	26.36064918593914	0.0
36	0.0	0.0	0.0	26.75966228923293	0.0
37	0.0	0.0	0.0	27.156741564885557	0.0
38	0.0	0.0	0.0	27.552692774414172	0.0
39	0.0	0.0	0.0	27.95154472540453	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGA	20	7.030774E-4	45.0	3
TCACGAG	25	3.8887243E-5	45.0	1
TCGCCGG	20	7.030774E-4	45.0	2
TTGATCG	20	7.030774E-4	45.0	20
CGGAATC	40	6.8066583E-9	45.0	6
CTATGCG	35	1.2107193E-7	45.0	1
TCGTTGC	20	7.030774E-4	45.0	38
TCCGCGG	90	0.0	45.0	2
CTCCGCC	45	3.8380676E-10	45.0	45
CAACGGA	45	3.8380676E-10	45.0	3
CGTGCTA	25	3.8887243E-5	45.0	17
CACGGAA	20	7.030774E-4	45.0	4
CGAAAAG	20	7.030774E-4	45.0	1
GCGAATG	40	6.8066583E-9	45.0	1
TTAGCGT	20	7.030774E-4	45.0	30
GAGTCGT	20	7.030774E-4	45.0	35
CCGGCAT	25	3.8887243E-5	45.0	19
GACGTGC	20	7.030774E-4	45.0	9
CGCGACG	20	7.030774E-4	45.0	1
TGACGGT	20	7.030774E-4	45.0	27
>>END_MODULE