FastQCFastQC Report
Sat 18 Jun 2016
SRR3548831_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548831_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339051
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGCT30020.8854125190605543TruSeq Adapter, Index 21 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27730.8178710577464746No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCC20620.6081680927058172No Hit
AATCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCT18440.5438709810618461No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGT17890.527649232711303No Hit
AAACTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCT11910.351274587009034No Hit
AAAAACTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTT7790.22975894481951092No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6800.2005597977885333No Hit
AAAAAACTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCT4870.14363620812208192No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4860.14334126724298116No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA4840.14275138548477956No Hit
AATGACTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTT3770.11119271142099565No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA3570.10529389383897997No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG3550.10470401208077841No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCAAC302.1609576E-645.00000419
GCGACGC302.1609576E-645.00000431
CGCATAG302.1609576E-645.0000041
TTCACGG302.1609576E-645.0000042
CCTACGG302.1609576E-645.0000042
CGTTCAC302.1609576E-645.00000421
TACATAA302.1609576E-645.00000419
AGACGTG302.1609576E-645.00000424
CTAACGA302.1609576E-645.00000422
ATATCCG302.1609576E-645.0000041
TACGAAA302.1609576E-645.00000420
CGAGGGC650.045.0000044
CATACCG302.1609576E-645.0000041
CGTAAGG302.1609576E-645.0000042
ACATACG302.1609576E-645.00000417
ATCATCG302.1609576E-645.00000440
TATCGAG302.1609576E-645.0000041
GCCAAAG302.1609576E-645.0000041
TGCGTGT302.1609576E-645.00000412
TGATCAG207.026245E-445.014
Produced by FastQC (version 0.11.5)