##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548831_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	339051
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20309039053122	31.0	31.0	34.0	28.0	34.0
2	31.41181120244447	31.0	31.0	34.0	28.0	34.0
3	31.404157486631803	31.0	31.0	34.0	28.0	34.0
4	35.423072045208535	37.0	35.0	37.0	33.0	37.0
5	35.092670424213466	37.0	35.0	37.0	32.0	37.0
6	35.142067122645265	37.0	35.0	37.0	32.0	37.0
7	35.06855310852939	37.0	35.0	37.0	32.0	37.0
8	35.46115186210924	37.0	35.0	37.0	33.0	37.0
9	35.99326060091255	39.0	37.0	39.0	32.0	39.0
10	35.81993564390018	38.0	35.0	39.0	30.0	39.0
11	36.18247402308207	38.0	35.0	39.0	32.0	39.0
12	35.74324511651639	37.0	35.0	39.0	31.0	39.0
13	35.29971892134222	37.0	35.0	39.0	30.0	39.0
14	36.26928102261902	38.0	35.0	40.0	31.0	41.0
15	36.43834408392838	38.0	35.0	40.0	31.0	41.0
16	36.54308348891465	38.0	35.0	40.0	31.0	41.0
17	36.482210640877035	38.0	35.0	40.0	31.0	41.0
18	36.41251021232794	38.0	35.0	40.0	31.0	41.0
19	36.38370038725738	38.0	35.0	40.0	31.0	41.0
20	36.33394091154428	38.0	35.0	40.0	31.0	41.0
21	36.309891432262404	38.0	35.0	40.0	31.0	41.0
22	36.33548935115956	38.0	35.0	40.0	31.0	41.0
23	36.26287785613374	38.0	35.0	40.0	31.0	41.0
24	35.84967158333112	37.0	34.0	40.0	30.0	41.0
25	35.98351575426706	38.0	34.0	40.0	30.0	41.0
26	35.87393636945474	38.0	34.0	40.0	30.0	41.0
27	35.77014667409917	38.0	34.0	40.0	30.0	41.0
28	35.666241951800764	37.0	34.0	40.0	29.0	41.0
29	35.54638092794299	37.0	34.0	40.0	29.0	41.0
30	35.48260586165503	37.0	34.0	40.0	29.0	41.0
31	35.37266664897022	37.0	34.0	40.0	29.0	41.0
32	35.306422927524174	37.0	34.0	40.0	29.0	41.0
33	35.184830600706086	37.0	34.0	40.0	28.0	41.0
34	35.12978283503072	37.0	34.0	40.0	27.0	41.0
35	35.07728630795957	37.0	34.0	40.0	27.0	41.0
36	34.86357214696314	37.0	34.0	40.0	27.0	41.0
37	34.70561655916072	37.0	33.0	40.0	26.0	41.0
38	34.68986081739915	36.0	33.0	40.0	27.0	41.0
39	34.64721826509876	36.0	33.0	40.0	26.0	41.0
40	34.392038365909556	36.0	33.0	40.0	25.0	41.0
41	34.46611571710451	36.0	33.0	40.0	25.0	41.0
42	34.45639151632055	36.0	33.0	40.0	25.0	41.0
43	34.41070517414784	36.0	33.0	40.0	25.0	41.0
44	34.35920554724805	36.0	33.0	40.0	25.0	41.0
45	34.180028963194324	35.0	33.0	39.0	25.0	41.0
46	33.98541222411968	35.0	33.0	39.0	24.0	41.0
47	33.837407941578114	35.0	33.0	39.0	23.0	40.0
48	33.76712353008839	35.0	33.0	39.0	23.0	40.0
49	33.61870633031609	35.0	33.0	39.0	23.0	40.0
50	33.47972723867501	35.0	33.0	39.0	23.0	40.0
51	31.72673432610433	34.0	30.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	4.0
11	5.0
12	6.0
13	6.0
14	15.0
15	37.0
16	62.0
17	126.0
18	290.0
19	624.0
20	871.0
21	1380.0
22	1878.0
23	2331.0
24	2906.0
25	3290.0
26	4027.0
27	4797.0
28	6073.0
29	7561.0
30	9686.0
31	12505.0
32	16916.0
33	23583.0
34	39141.0
35	40845.0
36	33642.0
37	43353.0
38	50170.0
39	32911.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.621826804816976	35.079088396730874	18.951426186620893	26.34765861183126
2	21.213032847565707	35.05903241695202	20.472141359264533	23.25579337621774
3	19.86898726150343	33.03219869577143	21.228664714158047	25.870149328567088
4	20.295471772683165	34.295725421839194	18.520812503133747	26.887990302343894
5	16.413164981079543	36.53078740366493	18.799826574763088	28.256221040492434
6	19.997581484791375	37.495244078324504	20.262143453344777	22.245030983539348
7	79.12025034581818	9.182099448165616	6.437674568132818	5.259975637883387
8	82.16875927220389	6.433545395825408	4.90811116911615	6.489584162854555
9	75.77001690011237	11.428664124276288	8.610209083589195	4.191109892022144
10	43.81228782690509	30.803920354165008	10.752659629377291	14.631132189552604
11	36.8201244060628	24.210517001866975	22.35504393144394	16.614314660626277
12	34.67767386027471	24.00671285440833	25.434816591014332	15.880796694302626
13	22.626979421974866	38.569713700888656	21.151685144712744	17.651621732423735
14	16.847317955115898	37.340105175917486	28.76646876133679	17.046108107629827
15	14.895694158106007	27.722083108440916	36.205467614016776	21.176755119436308
16	16.425257557122674	26.23469625513566	35.18792158111906	22.152124606622603
17	14.804262485584765	26.556476754234616	28.29633300005014	30.342927760130483
18	22.33587277430239	26.13146694745038	26.698048376202987	24.834611902044234
19	22.336167715181492	30.293967574199755	25.220394571908063	22.149470138710694
20	27.154616857051007	29.055510822855556	23.164361703696493	20.625510616396944
21	19.78964816502532	31.620906589274178	23.911741891337883	24.677703354362617
22	20.96970662230756	28.493353507289466	23.62093018454451	26.916009685858473
23	21.345166361402857	32.195451421762506	20.794511740121692	25.66487047671294
24	17.979595989983807	30.461493993528997	30.02645619685534	21.532453819631854
25	17.396202931122456	28.946087756709165	27.517689079224066	26.140020232944305
26	21.036363260984338	32.91038811270281	23.343980699068872	22.709267927243985
27	18.449436810391358	31.859808701345816	25.200338592129206	24.49041589613362
28	16.883890624124394	32.60158501228429	29.78814396654191	20.72638039704941
29	18.057460382066417	30.17481145904303	27.887544941616454	23.880183217274098
30	22.069541160474383	30.666477904504042	25.76927954791462	21.494701387106954
31	27.433041046922146	28.06332970556052	22.365661803091573	22.137967444425765
32	28.48715974882835	26.731081754662277	21.991971709270878	22.789786787238498
33	24.685371817219238	28.03177103149674	24.183382440989703	23.09947471029432
34	19.648961365694245	27.56163527021009	27.677252094817597	25.112151269278073
35	23.581408106745002	27.68639526206972	26.143559523493515	22.588637107691763
36	26.003462605920642	26.967624339701107	27.005081831346907	20.023831223031344
37	23.418010859723168	29.96864778455159	24.027358715945386	22.585982639779857
38	24.466820625805557	30.040908299931278	24.654697965792757	20.837573108470405
39	25.459296683979698	25.133387012573326	25.940050316913975	23.467265986533
40	27.727097103385628	25.24723419190623	22.40665858528658	24.61901011942156
41	20.739062854850747	22.506643543301745	28.186025111266446	28.56826849058106
42	26.539370183246767	25.327458111021645	22.53849715824463	25.594674547486957
43	23.254908553580435	25.571374218037995	25.77665306989214	25.397064158489428
44	22.857623189431678	26.481856711822115	24.454728049762426	26.205792048983785
45	23.94536515155537	29.149302022409607	23.877528749362188	23.02780407667283
46	20.53083459420559	26.037970688775435	29.95183615444284	23.47935856257613
47	23.235737396438882	26.128222597780276	26.485396002371324	24.150644003409518
48	23.44042636653483	21.76368746884687	31.99046751078746	22.805418653830838
49	22.86234224349729	22.544395975826646	29.31623855998065	25.277023220695412
50	20.281609551365428	27.411215421868683	25.19090048399798	27.116274542767904
51	20.450020793331976	25.139875711913547	28.89152369407552	25.518579800678953
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3223.0
1	2548.0
2	1873.0
3	1281.5
4	690.0
5	633.0
6	576.0
7	602.0
8	628.0
9	651.5
10	675.0
11	755.0
12	835.0
13	808.5
14	782.0
15	900.0
16	1018.0
17	964.0
18	910.0
19	988.0
20	1066.0
21	989.5
22	913.0
23	1025.0
24	1137.0
25	1253.0
26	1406.0
27	1443.0
28	1859.5
29	2276.0
30	2678.5
31	3081.0
32	4043.0
33	5005.0
34	5528.5
35	6052.0
36	6494.5
37	6937.0
38	7898.5
39	8860.0
40	11501.5
41	14143.0
42	18391.5
43	22640.0
44	28430.5
45	34221.0
46	34207.0
47	34193.0
48	33969.5
49	33746.0
50	32435.0
51	31124.0
52	29461.5
53	27799.0
54	24872.5
55	21946.0
56	19161.0
57	16376.0
58	14831.5
59	13287.0
60	12292.5
61	11298.0
62	10072.5
63	8847.0
64	7692.0
65	6537.0
66	5594.5
67	4652.0
68	3961.5
69	3271.0
70	2728.0
71	2185.0
72	1674.0
73	1163.0
74	1145.0
75	809.0
76	491.0
77	428.5
78	366.0
79	239.5
80	113.0
81	116.5
82	120.0
83	75.5
84	31.0
85	21.5
86	12.0
87	10.0
88	8.0
89	5.5
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	339051.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.00389140785779
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.13535213081073	23.74368511855433
2	9.297139157091719	5.579007073462448
3	3.0526299428564663	2.747723319415216
4	1.3583723057683748	1.6302582061486284
5	0.7756226632709717	1.1635849081127843
6	0.5377374539354454	0.9680529704290218
7	0.32284233440981636	0.6780568440444007
8	0.2798633214427755	0.6717590964489146
9	0.23188675190409058	0.6261754430746074
>10	3.677204138850109	27.669877120629653
>50	1.1944166746194311	24.509610444959307
>100	0.12893703014720218	5.7777280103830515
>500	0.001999023723212437	0.43754308975131695
>1k	0.00599707116963731	3.796938354586308
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGCT	3002	0.8854125190605543	TruSeq Adapter, Index 21 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2773	0.8178710577464746	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCC	2062	0.6081680927058172	No Hit
AATCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCT	1844	0.5438709810618461	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGT	1789	0.527649232711303	No Hit
AAACTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCT	1191	0.351274587009034	No Hit
AAAAACTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTT	779	0.22975894481951092	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	680	0.2005597977885333	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCT	487	0.14363620812208192	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	486	0.14334126724298116	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	484	0.14275138548477956	No Hit
AATGACTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTT	377	0.11119271142099565	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	357	0.10529389383897997	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	355	0.10470401208077841	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9494087910078424E-4	0.0	0.0	1.1502694284930586	0.0
2	2.9494087910078424E-4	0.0	0.0	1.4301683227597028	0.0
3	2.9494087910078424E-4	0.0	0.0	1.8162459335026293	0.0
4	2.9494087910078424E-4	0.0	0.0	4.126812780378173	0.0
5	2.9494087910078424E-4	0.0	0.0	4.441219757499609	0.0
6	2.9494087910078424E-4	0.0	0.0	5.975207269702788	0.0
7	2.9494087910078424E-4	0.0	0.0	7.217498252475291	0.0
8	2.9494087910078424E-4	0.0	0.0	7.923586717042569	0.0
9	2.9494087910078424E-4	0.0	0.0	9.473795977596291	0.0
10	2.9494087910078424E-4	0.0	0.0	11.018696302326198	0.0
11	2.9494087910078424E-4	0.0	0.0	13.215121028989739	0.0
12	2.9494087910078424E-4	0.0	0.0	14.049213835086757	0.0
13	2.9494087910078424E-4	0.0	0.0	14.413170879897125	0.0
14	2.9494087910078424E-4	0.0	0.0	14.829332460308331	0.0
15	2.9494087910078424E-4	0.0	0.0	15.165860003362326	0.0
16	2.9494087910078424E-4	0.0	0.0	15.715629802006188	0.0
17	2.9494087910078424E-4	0.0	0.0	16.439709660198613	0.0
18	2.9494087910078424E-4	0.0	0.0	17.381160946288315	0.0
19	2.9494087910078424E-4	0.0	0.0	17.75898021241642	0.0
20	2.9494087910078424E-4	0.0	0.0	18.140633709972835	0.0
21	2.9494087910078424E-4	0.0	0.0	18.572132216097284	0.0
22	2.9494087910078424E-4	0.0	0.0	18.96912263936694	0.0
23	2.9494087910078424E-4	0.0	0.0	19.410944076259913	0.0
24	2.9494087910078424E-4	0.0	0.0	19.68317450766994	0.0
25	2.9494087910078424E-4	0.0	0.0	19.9318096687519	0.0
26	2.9494087910078424E-4	0.0	0.0	20.176315657526448	0.0
27	2.9494087910078424E-4	0.0	0.0	20.419346941905495	0.0
28	2.9494087910078424E-4	0.0	0.0	20.66208328540544	0.0
29	2.9494087910078424E-4	0.0	0.0	20.91750208670672	0.0
30	2.9494087910078424E-4	0.0	0.0	21.32629014514041	0.0
31	2.9494087910078424E-4	0.0	0.0	21.604714335011547	0.0
32	2.9494087910078424E-4	0.0	0.0	21.843616447083182	0.0
33	2.9494087910078424E-4	0.0	0.0	22.108768297394786	0.0
34	2.9494087910078424E-4	0.0	0.0	22.364187098696068	0.0
35	2.9494087910078424E-4	0.0	0.0	22.68154348460851	0.0
36	2.9494087910078424E-4	0.0	0.0	22.941681339975403	0.0
37	2.9494087910078424E-4	0.0	0.0	23.175274516223222	0.0
38	2.9494087910078424E-4	0.0	0.0	23.4306933175245	0.0
39	2.9494087910078424E-4	0.0	0.0	23.720325260801474	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCAAC	30	2.1609576E-6	45.000004	19
GCGACGC	30	2.1609576E-6	45.000004	31
CGCATAG	30	2.1609576E-6	45.000004	1
TTCACGG	30	2.1609576E-6	45.000004	2
CCTACGG	30	2.1609576E-6	45.000004	2
CGTTCAC	30	2.1609576E-6	45.000004	21
TACATAA	30	2.1609576E-6	45.000004	19
AGACGTG	30	2.1609576E-6	45.000004	24
CTAACGA	30	2.1609576E-6	45.000004	22
ATATCCG	30	2.1609576E-6	45.000004	1
TACGAAA	30	2.1609576E-6	45.000004	20
CGAGGGC	65	0.0	45.000004	4
CATACCG	30	2.1609576E-6	45.000004	1
CGTAAGG	30	2.1609576E-6	45.000004	2
ACATACG	30	2.1609576E-6	45.000004	17
ATCATCG	30	2.1609576E-6	45.000004	40
TATCGAG	30	2.1609576E-6	45.000004	1
GCCAAAG	30	2.1609576E-6	45.000004	1
TGCGTGT	30	2.1609576E-6	45.000004	12
TGATCAG	20	7.026245E-4	45.0	14
>>END_MODULE