Basic Statistics
Measure | Value |
---|---|
Filename | SRR3548829_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 405506 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 48 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AATGATACGGCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCC | 5821 | 1.4354904736304765 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGT | 4974 | 1.2266156357735767 | No Hit |
AATCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCT | 4751 | 1.171622614708537 | No Hit |
CTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGCT | 3311 | 0.8165107298042447 | TruSeq Adapter, Index 13 (95% over 22bp) |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2364 | 0.5829753443845467 | No Hit |
AAACTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCT | 1043 | 0.257209511079984 | No Hit |
AATGACTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTT | 1026 | 0.2530172179943083 | No Hit |
AATGATCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCT | 891 | 0.21972547878453094 | No Hit |
AACTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTG | 765 | 0.18865318885540533 | No Hit |
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG | 683 | 0.1684315398539109 | No Hit |
AAAAACTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTT | 664 | 0.16374603581697927 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 589 | 0.1452506251448807 | No Hit |
AATGATACGGCGACTGTCTCTTATACACATCTGACGCATACGCACTCGTAT | 520 | 0.12823484732655002 | No Hit |
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA | 507 | 0.1250289761433863 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCACGGT | 20 | 7.0278795E-4 | 45.000004 | 41 |
ACGCGAG | 20 | 7.0278795E-4 | 45.000004 | 1 |
TATCACG | 20 | 7.0278795E-4 | 45.000004 | 1 |
TTAGGCG | 20 | 7.0278795E-4 | 45.000004 | 1 |
TCGTTAC | 20 | 7.0278795E-4 | 45.000004 | 36 |
TACCCCG | 20 | 7.0278795E-4 | 45.000004 | 1 |
CGAACAG | 20 | 7.0278795E-4 | 45.000004 | 1 |
ACGCCCA | 20 | 7.0278795E-4 | 45.000004 | 19 |
CACGGTA | 20 | 7.0278795E-4 | 45.000004 | 42 |
TAGACGC | 20 | 7.0278795E-4 | 45.000004 | 37 |
GAGTATC | 20 | 7.0278795E-4 | 45.000004 | 13 |
CATGACG | 40 | 6.7957444E-9 | 45.000004 | 1 |
TATGGAT | 20 | 7.0278795E-4 | 45.000004 | 4 |
ATTACGG | 80 | 0.0 | 45.000004 | 2 |
GCGCCTT | 20 | 7.0278795E-4 | 45.000004 | 16 |
AGGGTAC | 65 | 0.0 | 45.000004 | 6 |
CGATTCG | 20 | 7.0278795E-4 | 45.000004 | 1 |
CCGCTTA | 20 | 7.0278795E-4 | 45.000004 | 15 |
TAACGGT | 20 | 7.0278795E-4 | 45.000004 | 31 |
TCAGGTA | 20 | 7.0278795E-4 | 45.000004 | 10 |