FastQCFastQC Report
Sat 18 Jun 2016
SRR3548829_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548829_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405506
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCC58211.4354904736304765No Hit
AATGATACCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGT49741.2266156357735767No Hit
AATCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCT47511.171622614708537No Hit
CTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTGCT33110.8165107298042447TruSeq Adapter, Index 13 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23640.5829753443845467No Hit
AAACTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCT10430.257209511079984No Hit
AATGACTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTT10260.2530172179943083No Hit
AATGATCTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCT8910.21972547878453094No Hit
AACTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTTCTG7650.18865318885540533No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG6830.1684315398539109No Hit
AAAAACTGTCTCTTATACACATCTGACGCATACGCACTCGTATGCCGTCTT6640.16374603581697927No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5890.1452506251448807No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCATACGCACTCGTAT5200.12823484732655002No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA5070.1250289761433863No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGT207.0278795E-445.00000441
ACGCGAG207.0278795E-445.0000041
TATCACG207.0278795E-445.0000041
TTAGGCG207.0278795E-445.0000041
TCGTTAC207.0278795E-445.00000436
TACCCCG207.0278795E-445.0000041
CGAACAG207.0278795E-445.0000041
ACGCCCA207.0278795E-445.00000419
CACGGTA207.0278795E-445.00000442
TAGACGC207.0278795E-445.00000437
GAGTATC207.0278795E-445.00000413
CATGACG406.7957444E-945.0000041
TATGGAT207.0278795E-445.0000044
ATTACGG800.045.0000042
GCGCCTT207.0278795E-445.00000416
AGGGTAC650.045.0000046
CGATTCG207.0278795E-445.0000041
CCGCTTA207.0278795E-445.00000415
TAACGGT207.0278795E-445.00000431
TCAGGTA207.0278795E-445.00000410