SRR3548827_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548827_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	556365
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCC	5368	0.9648342365173942	No Hit
AATCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCT	4852	0.8720893657940382	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGT	4771	0.8575305779479299	No Hit
CTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGCT	3764	0.67653428954014	TruSeq Adapter, Index 15 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3090	0.5553907956107951	No Hit
AAACTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCT	1440	0.2588228950419239	No Hit
AATGACTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTT	1104	0.19843088619880836	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTT	1061	0.190702146971862	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCT	940	0.16895383426347813	No Hit
AACTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTG	796	0.1430715447592857	TruSeq Adapter, Index 21 (95% over 21bp)
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	759	0.13642123426168073	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	724	0.13013040000718953	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGTGGTATCGTAT	704	0.12653563757605168	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCT	577	0.10370889613832644	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	563	0.10119256243652998	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	559	0.10047360995030241	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACTACG	65	0.0	45.000004	1
GAGGCGT	65	0.0	45.000004	35
GTGCAAC	20	7.0301467E-4	45.0	30
CCTTACG	20	7.0301467E-4	45.0	1
TCCGCGT	20	7.0301467E-4	45.0	16
CTACTGA	25	3.8882026E-5	45.0	41
CTCCGAA	25	3.8882026E-5	45.0	18
CCTAGTG	25	3.8882026E-5	45.0	1
CCGATAG	20	7.0301467E-4	45.0	1
TCCGAAA	25	3.8882026E-5	45.0	19
CGGTTTA	40	6.8048394E-9	45.0	6
CCGGAAT	25	3.8882026E-5	45.0	5
TGAACGG	80	0.0	45.0	2
AAGCGTG	20	7.0301467E-4	45.0	1
CGACATA	20	7.0301467E-4	45.0	32
ATATGCG	20	7.0301467E-4	45.0	1
TACGGGT	40	6.8048394E-9	45.0	4
ACCCTTA	25	3.8882026E-5	45.0	22
ATGTCGA	20	7.0301467E-4	45.0	10
ATGTAGT	25	3.8882026E-5	45.0	20