##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548827_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	556365
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22695892085232	31.0	31.0	34.0	28.0	34.0
2	31.43958911865412	31.0	31.0	34.0	30.0	34.0
3	31.38265527126976	31.0	31.0	34.0	28.0	34.0
4	35.40617040971305	37.0	35.0	37.0	33.0	37.0
5	35.08471596883341	37.0	35.0	37.0	32.0	37.0
6	35.12807419589658	37.0	35.0	37.0	32.0	37.0
7	35.05783793013579	37.0	35.0	37.0	32.0	37.0
8	35.44687749948325	37.0	35.0	37.0	33.0	37.0
9	35.9604252603956	39.0	37.0	39.0	32.0	39.0
10	35.8364239303335	38.0	35.0	39.0	30.0	39.0
11	36.229588489570695	38.0	35.0	39.0	32.0	39.0
12	35.88684227081143	37.0	35.0	39.0	31.0	39.0
13	35.638210527261776	37.0	35.0	39.0	30.0	39.0
14	36.633454656565384	38.0	35.0	40.0	31.0	41.0
15	36.76511283060581	38.0	35.0	40.0	31.0	41.0
16	36.786190720120786	38.0	35.0	40.0	32.0	41.0
17	36.7359269544274	38.0	35.0	40.0	31.0	41.0
18	36.575266237092556	38.0	35.0	40.0	31.0	41.0
19	36.56683112704789	38.0	35.0	40.0	31.0	41.0
20	36.55766088808606	38.0	35.0	40.0	31.0	41.0
21	36.549693097157444	38.0	35.0	40.0	31.0	41.0
22	36.538459464560134	38.0	35.0	40.0	31.0	41.0
23	36.50109730123211	38.0	35.0	40.0	31.0	41.0
24	36.121869635940435	38.0	34.0	40.0	30.0	41.0
25	36.23688046516226	38.0	35.0	40.0	30.0	41.0
26	36.13834083739991	38.0	35.0	40.0	30.0	41.0
27	36.073068938556524	38.0	34.0	40.0	30.0	41.0
28	35.947805846881096	38.0	34.0	40.0	30.0	41.0
29	35.7761487512694	38.0	34.0	40.0	30.0	41.0
30	35.784813926109656	38.0	34.0	40.0	30.0	41.0
31	35.55681611891474	38.0	34.0	40.0	29.0	41.0
32	35.56852605753417	38.0	34.0	40.0	29.0	41.0
33	35.419059430409895	38.0	34.0	40.0	29.0	41.0
34	35.26424199940686	37.0	34.0	40.0	27.0	41.0
35	35.190628454342026	37.0	34.0	40.0	27.0	41.0
36	34.9890090138668	37.0	34.0	40.0	26.0	41.0
37	34.828010388863426	37.0	33.0	40.0	26.0	41.0
38	34.763462834649914	37.0	33.0	40.0	26.0	41.0
39	34.62123605906195	37.0	33.0	40.0	25.0	41.0
40	34.44474940012402	37.0	33.0	40.0	24.0	41.0
41	34.412815328067005	37.0	33.0	40.0	24.0	41.0
42	34.47865879413694	37.0	33.0	40.0	25.0	41.0
43	34.48952576096627	37.0	33.0	40.0	25.0	41.0
44	34.42495304341574	36.0	33.0	40.0	25.0	41.0
45	34.169271970738635	36.0	33.0	40.0	24.0	41.0
46	33.9910993682205	36.0	33.0	39.0	23.0	41.0
47	33.83587932382519	35.0	33.0	39.0	23.0	41.0
48	33.67379867533005	35.0	33.0	39.0	23.0	41.0
49	33.58247912791063	35.0	33.0	39.0	23.0	40.0
50	33.42361758917257	35.0	32.0	39.0	23.0	40.0
51	31.465045428810225	34.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	8.0
10	10.0
11	11.0
12	9.0
13	16.0
14	33.0
15	45.0
16	100.0
17	215.0
18	467.0
19	813.0
20	1333.0
21	2161.0
22	2954.0
23	3733.0
24	4595.0
25	5338.0
26	6234.0
27	7923.0
28	10015.0
29	12454.0
30	15911.0
31	20692.0
32	26917.0
33	37018.0
34	58331.0
35	62202.0
36	58353.0
37	74153.0
38	86201.0
39	58116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.873886747009607	35.20350848813279	19.4575503491413	24.4650544157163
2	23.078195069783327	35.55471677765495	18.955721513754458	22.41136663880726
3	20.861125340379065	30.346624967422464	22.991381557071346	25.800868135127118
4	22.78486245540248	31.652242682411725	19.101848606580212	26.46104625560558
5	17.45293107941729	36.55406073351127	18.11508631923288	27.877921867838555
6	20.254688918246117	36.70108651694481	21.793606715016224	21.250617849792853
7	77.79928643965742	10.13471372210689	6.782957231313976	5.283042606921716
8	80.66035785860002	5.844185022422331	5.3146765163157275	8.180780602661923
9	73.94174687480341	11.499105802845254	9.2390786623889	5.320068659962435
10	43.01780306094021	28.754864162914632	12.518400690194387	15.708932085950769
11	34.7068920582711	24.765576554959424	22.21760894376893	18.30992244300055
12	32.173123758683595	24.452472747207317	27.32163238161998	16.052771112489104
13	24.866589379274398	33.37647048250699	22.142298670836592	19.614641467382025
14	17.485463679419087	33.62972149578064	30.650382392853615	18.234432431946654
15	15.693834083739992	28.392332371734387	33.52996683831657	22.383866706209055
16	17.799466177778974	24.87216126104266	34.582153801910614	22.746218759267748
17	18.138272536913718	25.831243877670236	27.70842881921041	28.32205476620564
18	22.04452113270964	26.92890458601817	27.373936174993034	23.65263810627915
19	23.37152768416417	30.246510833715277	26.242304961670847	20.139656520449705
20	26.335768784880432	28.862527297727215	23.66144527423544	21.14025864315692
21	21.371761343722195	29.315826840293692	26.449543015825945	22.86286880015817
22	20.71230217572996	29.225598303272132	24.82596856380254	25.236130957195368
23	21.832609887394067	30.208586090066774	21.48715321776172	26.47165080477744
24	18.836015924797568	31.04257097409075	27.93507859049365	22.186334510618032
25	20.11970558895689	26.580032892076243	27.60364149434274	25.696620024624124
26	21.933802449830598	31.038436997294944	22.791333027778528	24.236427525095934
27	19.85009840662155	28.66715195959487	26.904280463364877	24.5784691704187
28	19.75951039335688	27.37771067554573	31.637324418322503	21.22545451277489
29	19.75663458341197	28.815975124243977	27.21918165233255	24.208208640011502
30	25.4144311737798	26.101749750613358	26.326602140681025	22.157216934925813
31	26.113252990393	27.37177931753435	23.85556244551688	22.65940524655577
32	28.123983356249944	25.351702569356448	23.093832286358776	23.430481788034832
33	27.38579889101579	25.773008726285802	25.11211165332111	21.729080729377298
34	21.24720282548327	25.560198790362442	27.474050308700228	25.718548075454063
35	23.593504264286935	25.85478957159419	27.45877256836789	23.092933595750992
36	26.69650319484511	24.763239959379185	28.214032155149948	20.326224690625757
37	24.611181508542053	28.75809944910266	24.250267360455815	22.380451681899473
38	25.189399045590577	28.533067320913432	25.472127110799565	20.805406522696433
39	24.206231520674375	24.89372983562949	28.067365847959525	22.83267279573661
40	25.529463571576212	27.211812389348715	24.142065011278568	23.1166590277965
41	20.554671843124567	22.821888508443198	31.190854924375184	25.43258472405705
42	24.744007980372597	26.14722349536725	24.353437042229473	24.75533148203068
43	23.203652278630035	24.38578990410971	27.083119894313985	25.327437922946267
44	23.375122446595313	23.903372785851015	25.63730644451035	27.084198323043324
45	24.9787459671259	27.340684622505012	24.80296208424326	22.877607326125833
46	21.312268025486865	25.64467570749418	30.422294716597914	22.620761550421037
47	22.75880042777673	26.59890539483972	25.579430769369026	25.062863408014525
48	22.215452086310247	22.48362136367313	31.956000107842875	23.34492644217375
49	22.845793678610267	22.47894817251265	30.09121709669012	24.584041052186965
50	21.352169888472496	24.44150872179235	25.87545945557323	28.330861934161927
51	20.38625722322576	24.88042921463428	29.399405066817646	25.333908495322316
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3683.0
1	2957.5
2	2232.0
3	1556.0
4	880.0
5	864.5
6	849.0
7	947.5
8	1046.0
9	1091.5
10	1137.0
11	1216.0
12	1295.0
13	1393.0
14	1491.0
15	1515.0
16	1539.0
17	1498.5
18	1458.0
19	1660.5
20	1863.0
21	1605.0
22	1347.0
23	1499.0
24	1651.0
25	1754.5
26	2518.0
27	3178.0
28	3670.0
29	4162.0
30	5290.5
31	6419.0
32	7186.0
33	7953.0
34	9060.0
35	10167.0
36	11871.5
37	13576.0
38	14569.0
39	15562.0
40	18605.0
41	21648.0
42	26826.0
43	32004.0
44	45107.5
45	58211.0
46	58846.5
47	59482.0
48	57933.5
49	56385.0
50	52612.0
51	48839.0
52	44986.0
53	41133.0
54	36004.5
55	30876.0
56	28207.0
57	25538.0
58	23701.0
59	21864.0
60	20616.0
61	19368.0
62	17696.5
63	16025.0
64	13977.0
65	11929.0
66	10863.0
67	9797.0
68	8753.0
69	7709.0
70	6134.0
71	4559.0
72	3775.0
73	2991.0
74	2562.5
75	1583.5
76	1033.0
77	854.0
78	675.0
79	562.0
80	449.0
81	318.0
82	187.0
83	154.5
84	122.0
85	75.5
86	29.0
87	19.0
88	9.0
89	9.0
90	9.0
91	9.5
92	10.0
93	5.5
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	556365.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.42926230716801
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.66788412468	23.15137797055106
2	9.359118229566352	5.508638906834121
3	3.267188388099461	2.884528322409374
4	1.5551845349855344	1.830717344645616
5	0.8569360292814752	1.2609497593093768
6	0.5722558672594344	1.0104640814636288
7	0.3961589334999724	0.8161065618509031
8	0.3220714383111577	0.7582659871764752
9	0.2695306076798324	0.7138878257898162
>10	3.355386999760966	24.380398711393095
>50	1.1758665115536164	24.16583425594374
>100	0.19260411300356578	7.909842500817524
>500	0.004907111159326517	1.0148599785384116
>1k	0.004293722264410702	3.625118834757805
>5k	6.133888949158146E-4	0.9690089585190668
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCC	5368	0.9648342365173942	No Hit
AATCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCT	4852	0.8720893657940382	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGT	4771	0.8575305779479299	No Hit
CTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGCT	3764	0.67653428954014	TruSeq Adapter, Index 15 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3090	0.5553907956107951	No Hit
AAACTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCT	1440	0.2588228950419239	No Hit
AATGACTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTT	1104	0.19843088619880836	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTT	1061	0.190702146971862	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCT	940	0.16895383426347813	No Hit
AACTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTG	796	0.1430715447592857	TruSeq Adapter, Index 21 (95% over 21bp)
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	759	0.13642123426168073	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	724	0.13013040000718953	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGTGGTATCGTAT	704	0.12653563757605168	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCT	577	0.10370889613832644	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	563	0.10119256243652998	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	559	0.10047360995030241	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.5947624311378324E-4	0.0	0.0	0.9281676597197883	0.0
2	3.5947624311378324E-4	0.0	0.0	1.1767454818329695	0.0
3	3.5947624311378324E-4	0.0	0.0	1.6508946465000494	0.0
4	3.5947624311378324E-4	0.0	0.0	4.18933613724803	0.0
5	3.5947624311378324E-4	0.0	0.0	4.541263379256423	0.0
6	3.5947624311378324E-4	0.0	0.0	6.102648441221141	0.0
7	3.5947624311378324E-4	0.0	0.0	7.293593234657105	0.0
8	3.5947624311378324E-4	0.0	0.0	7.925013255686465	0.0
9	3.5947624311378324E-4	0.0	0.0	9.855220943085923	0.0
10	3.5947624311378324E-4	0.0	0.0	11.252864576312312	0.0
11	3.5947624311378324E-4	0.0	0.0	13.899328678115985	0.0
12	3.5947624311378324E-4	0.0	0.0	14.719293988658524	0.0
13	3.5947624311378324E-4	0.0	0.0	15.109685188680093	0.0
14	3.5947624311378324E-4	0.0	0.0	15.584912782076515	0.0
15	3.5947624311378324E-4	0.0	0.0	15.88813099314299	0.0
16	3.5947624311378324E-4	0.0	0.0	16.453766861682528	0.0
17	3.5947624311378324E-4	0.0	0.0	17.082670549010093	0.0
18	3.5947624311378324E-4	0.0	0.0	17.956377557898143	0.0
19	3.5947624311378324E-4	0.0	0.0	18.307765585541866	0.0
20	3.5947624311378324E-4	0.0	0.0	18.69546071374008	0.0
21	3.5947624311378324E-4	0.0	0.0	19.114430275089195	0.0
22	3.5947624311378324E-4	0.0	0.0	19.54436386185328	0.0
23	3.5947624311378324E-4	0.0	0.0	19.988496760220357	0.0
24	3.5947624311378324E-4	0.0	0.0	20.304835854160487	0.0
25	3.5947624311378324E-4	0.0	0.0	20.558266605555705	0.0
26	3.5947624311378324E-4	0.0	0.0	20.80378887960242	0.0
27	3.5947624311378324E-4	0.0	0.0	21.0719581569653	0.0
28	3.5947624311378324E-4	0.0	0.0	21.330241837642554	0.0
29	3.5947624311378324E-4	0.0	0.0	21.599130067491664	0.0
30	3.5947624311378324E-4	0.0	0.0	22.01522381889587	0.0
31	3.5947624311378324E-4	0.0	0.0	22.313768838801867	0.0
32	3.5947624311378324E-4	0.0	0.0	22.577085186882712	0.0
33	3.5947624311378324E-4	0.0	0.0	22.868260943804877	0.0
34	3.5947624311378324E-4	0.0	0.0	23.11719824216117	0.0
35	3.5947624311378324E-4	0.0	0.0	23.45726276814681	0.0
36	3.5947624311378324E-4	0.0	0.0	23.727049688603703	0.0
37	3.5947624311378324E-4	0.0	0.0	23.990006560441437	0.0
38	3.5947624311378324E-4	0.0	0.0	24.26051243338456	0.0
39	3.5947624311378324E-4	0.0	0.0	24.556720857710317	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACTACG	65	0.0	45.000004	1
GAGGCGT	65	0.0	45.000004	35
GTGCAAC	20	7.0301467E-4	45.0	30
CCTTACG	20	7.0301467E-4	45.0	1
TCCGCGT	20	7.0301467E-4	45.0	16
CTACTGA	25	3.8882026E-5	45.0	41
CTCCGAA	25	3.8882026E-5	45.0	18
CCTAGTG	25	3.8882026E-5	45.0	1
CCGATAG	20	7.0301467E-4	45.0	1
TCCGAAA	25	3.8882026E-5	45.0	19
CGGTTTA	40	6.8048394E-9	45.0	6
CCGGAAT	25	3.8882026E-5	45.0	5
TGAACGG	80	0.0	45.0	2
AAGCGTG	20	7.0301467E-4	45.0	1
CGACATA	20	7.0301467E-4	45.0	32
ATATGCG	20	7.0301467E-4	45.0	1
TACGGGT	40	6.8048394E-9	45.0	4
ACCCTTA	25	3.8882026E-5	45.0	22
ATGTCGA	20	7.0301467E-4	45.0	10
ATGTAGT	25	3.8882026E-5	45.0	20
>>END_MODULE