##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548826_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	404254
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.39432881307297	31.0	31.0	34.0	30.0	34.0
2	31.569706174830674	31.0	31.0	34.0	30.0	34.0
3	31.760150796281547	31.0	31.0	34.0	30.0	34.0
4	35.58210926793551	37.0	35.0	37.0	33.0	37.0
5	35.18181885646153	37.0	35.0	37.0	32.0	37.0
6	35.200096968737476	37.0	35.0	37.0	32.0	37.0
7	35.1870902947157	37.0	35.0	37.0	32.0	37.0
8	35.50654044239513	37.0	35.0	37.0	33.0	37.0
9	36.35874722328041	39.0	37.0	39.0	32.0	39.0
10	36.0955117327225	38.0	35.0	39.0	31.0	39.0
11	36.327581174212256	38.0	35.0	39.0	32.0	39.0
12	35.96777026325033	37.0	35.0	39.0	32.0	39.0
13	35.742105211080165	37.0	35.0	39.0	30.0	39.0
14	36.77055267232977	38.0	35.0	40.0	31.0	41.0
15	36.81703829770392	38.0	35.0	40.0	31.0	41.0
16	36.803714991069974	38.0	35.0	40.0	31.0	41.0
17	36.7622014871838	38.0	35.0	40.0	31.0	41.0
18	36.61925917863521	38.0	35.0	40.0	31.0	41.0
19	36.61985039109075	38.0	35.0	40.0	31.0	41.0
20	36.63053179436691	38.0	35.0	40.0	31.0	41.0
21	36.69284410296497	38.0	35.0	40.0	31.0	41.0
22	36.66366442879971	38.0	35.0	40.0	31.0	41.0
23	36.614141109302565	38.0	35.0	40.0	31.0	41.0
24	36.27042404033108	38.0	35.0	40.0	30.0	41.0
25	36.33295403385001	38.0	35.0	40.0	30.0	41.0
26	36.2187189242407	38.0	35.0	40.0	30.0	41.0
27	36.18434449628204	38.0	35.0	40.0	30.0	41.0
28	36.149707856941426	38.0	35.0	40.0	30.0	41.0
29	35.85005961598401	38.0	34.0	40.0	30.0	41.0
30	35.93182998807681	38.0	35.0	40.0	30.0	41.0
31	35.77621000658002	38.0	34.0	40.0	29.0	41.0
32	35.71147100585276	38.0	34.0	40.0	29.0	41.0
33	35.717820973942125	38.0	34.0	40.0	29.0	41.0
34	35.58636154496925	38.0	34.0	40.0	29.0	41.0
35	35.58778391803173	38.0	34.0	40.0	29.0	41.0
36	35.24548922212273	37.0	34.0	40.0	27.0	41.0
37	35.20384955993014	37.0	34.0	40.0	27.0	41.0
38	35.14488415699041	37.0	34.0	40.0	27.0	41.0
39	34.98173920357003	37.0	34.0	40.0	27.0	41.0
40	34.81014906469695	37.0	33.0	40.0	26.0	41.0
41	34.77339989214702	37.0	33.0	40.0	26.0	41.0
42	34.686231923493644	37.0	33.0	40.0	26.0	41.0
43	34.47316043873406	37.0	33.0	40.0	24.0	41.0
44	34.42178927110183	37.0	33.0	40.0	24.0	41.0
45	34.08399669514711	36.0	33.0	40.0	24.0	41.0
46	33.917492467607	36.0	33.0	39.0	23.0	41.0
47	33.790431263512545	36.0	33.0	39.0	23.0	41.0
48	33.71753649932963	36.0	33.0	39.0	23.0	41.0
49	33.595642838413475	36.0	33.0	39.0	23.0	41.0
50	33.277894591024456	35.0	32.0	39.0	22.0	40.0
51	31.391540961870508	34.0	29.0	38.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	2.0
11	9.0
12	5.0
13	14.0
14	20.0
15	48.0
16	72.0
17	144.0
18	304.0
19	559.0
20	892.0
21	1249.0
22	1926.0
23	2345.0
24	2986.0
25	3626.0
26	4610.0
27	5690.0
28	7116.0
29	9050.0
30	11512.0
31	14865.0
32	19073.0
33	25710.0
34	40437.0
35	43756.0
36	41271.0
37	53768.0
38	66538.0
39	46649.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.485635268915086	34.79569775438215	19.72695384584939	24.991713130853373
2	23.79271448148936	34.230459067813804	18.955656592142564	23.021169858554273
3	20.827252173138668	30.736121349448613	22.81436918373102	25.622257293681695
4	23.72097740529469	31.34638123555982	18.590786980462777	26.34185437868271
5	17.399704146403003	36.68263022753022	18.34218090606401	27.57548472000277
6	20.90171031084417	36.44490839917477	21.578265150128384	21.075116139852668
7	78.4427612342735	9.192982629732791	6.8348117767542185	5.529444359239488
8	80.79450048731738	5.298896238503515	5.198959070287493	8.707644203891613
9	73.90972012645514	10.305154679978429	10.006580021471649	5.778545172094772
10	42.44608587670128	28.060823145843948	12.719973086227965	16.773117891226804
11	33.467572367867724	24.929870823789006	22.33793605010711	19.26462075823616
12	31.520529172253088	23.357344639756192	28.594151201967076	16.52797498602364
13	25.00185526921193	31.83864599979221	22.48437863323554	20.67512009776032
14	19.11916765202076	32.05707302834357	30.556778658961942	18.266980660673735
15	17.28814062445888	28.20602888283109	31.47649744962326	23.029333043086773
16	18.16061189252302	24.598643427152236	33.10789750998135	24.132847170343396
17	18.328575598509847	25.831779030015785	28.639172401509942	27.20047296996443
18	23.055059442825552	24.65232254968411	26.500418053995755	25.79219995349459
19	22.212272482152308	30.627031519787064	27.004061802727986	20.156634195332636
20	27.03894086391229	28.327487173905514	23.51392936124318	21.119642600939017
21	22.22290935896738	28.167934021679443	25.674204831615764	23.93495178773741
22	20.950689418039154	27.179941324019058	25.15225575999248	26.717113497949306
23	22.37553617280225	28.468240264784022	22.492047079311522	26.66417648310221
24	19.67302735408926	30.729689749513923	26.732697759329533	22.86458513706729
25	20.785941512019672	25.303150989229543	27.086683124965987	26.8242243737848
26	23.96166766438922	30.34552533803005	22.39408886492156	23.298718132659168
27	19.153057236292035	27.23832046188782	26.501902269365303	27.106720032454845
28	20.052986488692753	27.343205014668996	30.83407956383858	21.769728932799676
29	19.6792115847957	31.83270913831403	25.638831031974945	22.849248244915326
30	22.507631340691745	26.266406764064175	26.720329297916656	24.505632597327423
31	27.49805815155818	28.68048306262894	22.498726048474474	21.322732737338406
32	25.740252415560516	28.23249739025464	21.732376179332796	24.29487401485205
33	26.710434528786358	24.79752828667125	25.040939607276613	23.451097577265777
34	21.079074047504786	28.358903065894214	25.649715278018277	24.912307608582722
35	24.436616582643587	24.446263982545627	27.46342645960213	23.653692975208656
36	25.964863674818307	26.751745189905357	27.18835187777981	20.095039257496524
37	23.048875212119114	30.338351630410582	22.170714451805054	24.44205870566525
38	25.79986839957057	27.68902719577295	24.765617656226034	21.745486748430444
39	24.71688591825931	26.116006273283627	25.18070322124209	23.986404587214967
40	28.013575623246773	25.312551019903328	22.05123511455669	24.622638242293213
41	24.053441648072745	21.705907671909245	28.913999614104004	25.326651065914003
42	27.463179090373874	23.8518357270429	23.135949180465747	25.549036002117482
43	23.948309726063314	23.338297209180368	26.366591301508457	26.346801763247857
44	22.454446956616383	22.555373601745437	25.293503589327504	29.696675852310676
45	26.788355835687465	26.031653366447827	25.079776576113037	22.10021422175167
46	22.227362005076017	24.69041741083576	30.425672967985474	22.65654761610275
47	23.428834346722603	25.77241041523399	25.175508467448683	25.623246770594726
48	23.05802787356464	21.997555992024818	31.82875123066191	23.115664903748634
49	24.172178877636338	21.56490721180248	29.127973996546725	25.134939914014453
50	21.745981486886954	23.886467418998947	25.749899815462555	28.61765127865154
51	21.50232279705334	23.10156485773796	29.565322792105952	25.83078955310275
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2339.0
1	1788.5
2	1238.0
3	867.5
4	497.0
5	451.5
6	406.0
7	447.5
8	489.0
9	495.5
10	502.0
11	536.0
12	570.0
13	661.5
14	753.0
15	757.5
16	762.0
17	834.5
18	907.0
19	916.0
20	925.0
21	882.0
22	839.0
23	848.5
24	858.0
25	1016.5
26	1520.5
27	1866.0
28	2364.5
29	2863.0
30	3172.5
31	3482.0
32	4014.0
33	4546.0
34	5470.5
35	6395.0
36	7565.5
37	8736.0
38	9516.0
39	10296.0
40	12496.5
41	14697.0
42	18392.5
43	22088.0
44	30914.0
45	39740.0
46	42599.5
47	45459.0
48	43378.5
49	41298.0
50	38752.0
51	36206.0
52	33793.5
53	31381.0
54	27695.5
55	24010.0
56	22067.5
57	20125.0
58	18633.0
59	17141.0
60	16439.5
61	15738.0
62	14645.5
63	13553.0
64	12173.5
65	10794.0
66	9209.0
67	7624.0
68	6244.5
69	4865.0
70	4093.5
71	3322.0
72	2820.5
73	2319.0
74	1856.5
75	1281.0
76	1168.0
77	782.5
78	397.0
79	347.5
80	298.0
81	195.0
82	92.0
83	73.0
84	54.0
85	34.5
86	15.0
87	13.5
88	12.0
89	11.0
90	10.0
91	7.0
92	4.0
93	4.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	404254.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.54385802504366
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.47080682414686	24.273450407724127
2	8.447522030590202	5.160398271315514
3	2.7177473863142265	2.4903147094654567
4	1.3076530184060857	1.5976307256086113
5	0.7918202742398317	1.2092623018866278
6	0.5464528374664661	1.00144667309748
7	0.4324181265028296	0.9245402504350458
8	0.33864994124677195	0.8274940580504625
9	0.2958114519331493	0.8131700691025325
>10	4.669931443565414	35.21828490367014
>50	0.8987475882487956	17.530269288382776
>100	0.07435681485511618	3.9295728402590693
>500	0.004041131242125879	0.969679740956001
>1k	0.004041131242125879	4.0544857600461714
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCC	4498	1.1126667887021526	No Hit
AATCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCT	3428	0.8479817144666472	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGT	3320	0.8212658378148392	No Hit
CTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGCT	3176	0.7856446689457619	Illumina Single End Adapter 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2002	0.49523319497147833	No Hit
AAACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCT	954	0.23599024375763755	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	926	0.229063905366428	No Hit
AATGACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTT	762	0.18849535193220104	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCT	653	0.16153210605213553	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTT	633	0.1565847214869859	No Hit
AACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTG	470	0.1162635372810164	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	450	0.11131615271586676	No Hit
TGCGAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	439	0.10859509120503445	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTAT	421	0.10414244509639979	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	419	0.10364770663988483	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.473692282574817E-4	0.0	0.0	1.1848986033533373	0.0
2	2.473692282574817E-4	0.0	0.0	1.458983708262627	0.0
3	2.473692282574817E-4	0.0	0.0	1.9539695340058478	0.0
4	2.473692282574817E-4	0.0	0.0	4.733162813478654	0.0
5	2.473692282574817E-4	0.0	0.0	5.052516487159063	0.0
6	2.473692282574817E-4	0.0	0.0	6.5439055643234205	0.0
7	2.473692282574817E-4	0.0	0.0	7.745872644426524	0.0
8	2.473692282574817E-4	0.0	0.0	8.354895684396444	0.0
9	2.473692282574817E-4	0.0	0.0	10.403855002053165	0.0
10	2.473692282574817E-4	0.0	0.0	11.879907187065557	0.0
11	2.473692282574817E-4	0.0	0.0	14.638568820592994	0.0
12	2.473692282574817E-4	0.0	0.0	15.417781889604061	0.0
13	2.473692282574817E-4	0.0	0.0	15.759398793827643	0.0
14	2.473692282574817E-4	0.0	0.0	16.18710018948483	0.0
15	2.473692282574817E-4	0.0	0.0	16.5002696324588	0.0
16	2.473692282574817E-4	0.0	0.0	17.045224042310032	0.0
17	2.473692282574817E-4	0.0	0.0	17.68492086658388	0.0
18	2.473692282574817E-4	0.0	0.0	18.590044872778005	0.0
19	2.473692282574817E-4	0.0	0.0	18.961840822849002	0.0
20	2.473692282574817E-4	0.0	0.0	19.327947280670074	0.0
21	2.473692282574817E-4	0.0	0.0	19.718543292088636	0.0
22	2.473692282574817E-4	0.0	0.0	20.152181549224004	0.0
23	2.473692282574817E-4	0.0	0.0	20.6083304061308	0.0
24	2.473692282574817E-4	0.0	0.0	20.9422788642784	0.0
25	0.00148421536954489	0.0	0.0	21.19954286166618	0.0
26	0.00148421536954489	0.0	0.0	21.432812043912985	0.0
27	0.00148421536954489	0.0	0.0	21.674739149148802	0.0
28	0.00148421536954489	0.0	0.0	21.932003146536584	0.0
29	0.00148421536954489	0.0	0.0	22.188277667011334	0.0
30	0.00148421536954489	0.0	0.0	22.56205257090839	0.0
31	0.00148421536954489	0.0	0.0	22.82649027591564	0.0
32	0.00148421536954489	0.0	0.0	23.082270057933872	0.0
33	0.00148421536954489	0.0	0.0	23.384307885636257	0.0
34	0.00148421536954489	0.0	0.0	23.634150806176315	0.0
35	0.00148421536954489	0.0	0.0	23.948557095291573	0.0
36	0.00148421536954489	0.0	0.0	24.203594769625038	0.0
37	0.00148421536954489	0.0	0.0	24.45962192087153	0.0
38	0.00148421536954489	0.0	0.0	24.749291287161043	0.0
39	0.00148421536954489	0.0	0.0	25.016202684450864	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCATCGG	35	1.2093187E-7	45.000004	2
TCATAGT	35	1.2093187E-7	45.000004	28
ACGACAG	35	1.2093187E-7	45.000004	1
GTCATCG	25	3.8863032E-5	45.0	1
AATCCGG	75	0.0	45.0	2
TACCCCG	25	3.8863032E-5	45.0	1
CGAACGG	40	6.7957444E-9	45.0	2
AATCACG	20	7.0278544E-4	45.0	27
CGAAATA	20	7.0278544E-4	45.0	16
CGTGCTC	20	7.0278544E-4	45.0	45
GAGTCAT	25	3.8863032E-5	45.0	17
TGAACTT	25	3.8863032E-5	45.0	16
CCTACGG	20	7.0278544E-4	45.0	2
ATATGCG	25	3.8863032E-5	45.0	1
TAACGCT	20	7.0278544E-4	45.0	16
TAACGAC	20	7.0278544E-4	45.0	29
ATCCGAA	20	7.0278544E-4	45.0	22
GTTACGG	40	6.7957444E-9	45.0	2
CCGTATA	20	7.0278544E-4	45.0	28
ATATCGC	25	3.8863032E-5	45.0	38
>>END_MODULE