SRR3548825_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548825_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	411841
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCC	4262	1.0348653970828547	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGT	3420	0.8304175640599163	No Hit
AATCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCT	3231	0.7845260670987104	No Hit
CTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGCT	3070	0.7454333104280535	Illumina Single End Adapter 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2111	0.5125764554767496	No Hit
AAACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCT	925	0.22460124174135165	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	924	0.22435842958811775	No Hit
AATGACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTT	790	0.191821601054776	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCT	623	0.15127197146471574	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTT	552	0.1340323085851093	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	510	0.12383419814928577	No Hit
TGCGAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	469	0.11387889986669614	No Hit
AACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTG	455	0.11047952972142161	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	442	0.107322971729381	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTAT	425	0.1031951651244048	No Hit

Adapter graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCC	20	7.028007E-4	45.000004	13
CCTTCAA	20	7.028007E-4	45.000004	34
TTAGGGT	20	7.028007E-4	45.000004	4
ATGATCG	20	7.028007E-4	45.000004	27
AATCACG	20	7.028007E-4	45.000004	27
ACGCACG	20	7.028007E-4	45.000004	1
TTTCGCA	20	7.028007E-4	45.000004	13
CGTTGTA	20	7.028007E-4	45.000004	28
CGGTTAC	20	7.028007E-4	45.000004	37
TAAACCG	35	1.2093915E-7	45.000004	1
ACTTCGC	20	7.028007E-4	45.000004	12
GGTCATA	20	7.028007E-4	45.000004	8
CACTGGT	20	7.028007E-4	45.000004	28
CGACACG	35	1.2093915E-7	45.000004	1
ACGGGTC	20	7.028007E-4	45.000004	5
TGACCGG	20	7.028007E-4	45.000004	2
GTTAATA	20	7.028007E-4	45.000004	36
TAACACG	20	7.028007E-4	45.000004	1
TTGACGT	20	7.028007E-4	45.000004	33
CGGGCAA	45	3.8380676E-10	45.000004	6