##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548825_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	411841
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1902918844894	31.0	31.0	34.0	28.0	34.0
2	31.410386532666735	31.0	31.0	34.0	30.0	34.0
3	31.373981706532376	31.0	31.0	34.0	28.0	34.0
4	35.37913175230246	37.0	35.0	37.0	33.0	37.0
5	35.044730854868746	37.0	35.0	37.0	32.0	37.0
6	35.11744338227617	37.0	35.0	37.0	32.0	37.0
7	35.0496453728502	37.0	35.0	37.0	32.0	37.0
8	35.4336527931896	37.0	35.0	37.0	33.0	37.0
9	35.84697735291047	39.0	37.0	39.0	32.0	39.0
10	35.75756420560362	38.0	35.0	39.0	30.0	39.0
11	36.165085554862195	38.0	35.0	39.0	32.0	39.0
12	35.86795632294988	37.0	35.0	39.0	31.0	39.0
13	35.627380469647264	37.0	35.0	39.0	30.0	39.0
14	36.64504505379503	38.0	35.0	40.0	31.0	41.0
15	36.72316015161191	38.0	35.0	40.0	31.0	41.0
16	36.73081844692491	38.0	35.0	40.0	31.0	41.0
17	36.69081757280115	38.0	35.0	40.0	31.0	41.0
18	36.57056485391207	38.0	35.0	40.0	31.0	41.0
19	36.558278558958435	38.0	35.0	40.0	31.0	41.0
20	36.545011302905735	38.0	35.0	40.0	31.0	41.0
21	36.580546861531516	38.0	35.0	40.0	31.0	41.0
22	36.58863493435573	38.0	35.0	40.0	31.0	41.0
23	36.54154880160062	38.0	35.0	40.0	31.0	41.0
24	36.15925077881998	38.0	35.0	40.0	30.0	41.0
25	36.262237125492604	38.0	35.0	40.0	30.0	41.0
26	36.14371808537761	38.0	35.0	40.0	30.0	41.0
27	36.118441340225964	38.0	35.0	40.0	30.0	41.0
28	36.05251055625836	38.0	35.0	40.0	30.0	41.0
29	35.77309204280293	38.0	34.0	40.0	29.0	41.0
30	35.88157565662477	38.0	34.0	40.0	30.0	41.0
31	35.76857573675277	38.0	34.0	40.0	29.0	41.0
32	35.70171498223829	38.0	34.0	40.0	29.0	41.0
33	35.65216187800632	38.0	34.0	40.0	29.0	41.0
34	35.59127673058292	38.0	34.0	40.0	29.0	41.0
35	35.553298481695606	38.0	34.0	40.0	29.0	41.0
36	35.277298763357706	37.0	34.0	40.0	28.0	41.0
37	35.22116787789462	37.0	34.0	40.0	27.0	41.0
38	35.14845292236567	37.0	34.0	40.0	27.0	41.0
39	34.98442360037004	37.0	34.0	40.0	27.0	41.0
40	34.78869029552667	37.0	33.0	40.0	26.0	41.0
41	34.75893609426939	37.0	33.0	40.0	26.0	41.0
42	34.74109911349283	37.0	33.0	40.0	26.0	41.0
43	34.56584215753167	37.0	33.0	40.0	25.0	41.0
44	34.59271175040853	37.0	33.0	40.0	26.0	41.0
45	34.29974189068111	36.0	33.0	40.0	24.0	41.0
46	34.036249426356285	36.0	33.0	39.0	23.0	41.0
47	33.89779793658232	36.0	33.0	39.0	23.0	41.0
48	33.79163075070233	35.0	33.0	39.0	23.0	41.0
49	33.67321126357017	36.0	33.0	39.0	23.0	40.0
50	33.466803936470626	35.0	32.0	39.0	23.0	40.0
51	31.68202291661102	35.0	29.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	2.0
11	3.0
12	9.0
13	13.0
14	21.0
15	45.0
16	65.0
17	166.0
18	328.0
19	540.0
20	908.0
21	1343.0
22	1918.0
23	2441.0
24	3013.0
25	3650.0
26	4643.0
27	5658.0
28	7320.0
29	9399.0
30	11952.0
31	15230.0
32	19659.0
33	27182.0
34	42113.0
35	45150.0
36	43243.0
37	55629.0
38	66354.0
39	43838.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.640247085647133	34.509434466213904	19.78530549411059	25.065012954028376
2	24.107361821673898	33.84291510558686	18.925507659509375	23.124215413229862
3	21.196286916552747	30.736133604959193	22.475178527635663	25.59240095085239
4	23.85362312154448	31.469426307725556	18.511027313939117	26.165923256790848
5	17.513069364147814	36.64836672405127	18.084649172860402	27.753914738940516
6	20.759710665038206	36.59009180727514	21.370140418268218	21.28005710941844
7	78.99407781158263	9.205008729096908	6.4653592041588865	5.335554255161579
8	81.47926019993153	5.370762017380494	4.946811997834115	8.203165784853864
9	73.65658105919518	11.367979390104434	9.426939037152687	5.548500513547705
10	42.945942730325534	28.195104421366494	12.420812886526598	16.438139961781367
11	33.713253415759965	25.255620494316982	21.775151089862348	19.255975000060705
12	31.962820603096826	23.366299130004055	28.16693821159137	16.503942055307753
13	25.053843594979615	32.00312742053365	22.44846919077994	20.494559793706795
14	19.332218016176146	32.300815120398404	30.07082830509833	18.296138558327122
15	17.396033906289077	28.170580393889878	31.671446019216155	22.761939680604893
16	18.4163305741779	24.5708902221974	33.219130683928995	23.793648519695708
17	18.510784501785885	25.666944281895198	28.43913063536656	27.383140580952357
18	23.238822749556263	24.71050721030689	26.640378204209874	25.410291835926973
19	22.838911133180037	30.445730269691456	26.596186392321307	20.119172204807196
20	27.261491692182176	28.414606607889937	23.257519285355272	21.066382414572615
21	22.404034566738133	28.28203117222423	25.600170939755877	23.713763321281757
22	21.023890287756682	27.09637942798313	25.0028530428005	26.87687724145969
23	22.261989457096306	28.735847086618378	22.601926471623756	26.400236984661557
24	19.923708421453913	30.341806668107353	27.06651353313536	22.667971377303378
25	20.84857991312181	25.522956675027498	27.06141447791745	26.56704893393324
26	23.737801724451913	30.586804130720353	22.26028977202367	23.41510437280407
27	19.248447823310453	27.59560121503202	26.432045376735196	26.723905584922335
28	20.10387503915346	27.594387154265842	30.74633171539502	21.555406091185674
29	19.678953770994145	31.78581054338932	25.757027590744975	22.778208094871562
30	22.444584196328194	26.613426055200918	26.87614880499999	24.065840943470903
31	27.572291248321562	28.806505423209444	22.290398479024674	21.330804849444323
32	25.93913670567039	28.19534723351973	21.753783620377767	24.11173244043211
33	26.623624165636738	25.141255970143817	24.938265010040283	23.29685485417916
34	20.887429857639233	28.74968737935271	25.499161084010574	24.86372167899748
35	24.123387423787335	24.577931774641186	27.701710125995227	23.596970675576255
36	26.054715290609725	26.991727389939324	26.987842395487576	19.965714923963375
37	22.937492867393	30.637309058592997	22.3450312135023	24.080166860511703
38	25.48313548189714	28.02295060472367	24.551222437785455	21.942691475593737
39	24.70346565786311	26.37352764780583	24.838712027214385	24.08429466711668
40	27.88090549508184	25.50231764200262	22.019905740322116	24.596871122593427
41	23.778108541888738	22.00970762988629	28.752843937344753	25.459339890880216
42	27.1706799468727	23.976243258927596	23.14145507610947	25.711621718090232
43	23.89854336989275	23.493047073992148	26.270575294834657	26.337834261280445
44	22.412290179948087	22.955946591038774	25.383582499071245	29.248180729941897
45	26.3890676256128	26.645234447274557	25.053843594979615	21.911854332133032
46	22.19157393265848	25.37095626710308	30.00381215080577	22.43365764943267
47	23.359500389713507	26.171022312008763	25.082981053367682	25.38649624491005
48	23.14509725840798	22.30690970544458	31.461413506668833	23.08657952947861
49	24.206186368040093	22.016749182330074	28.96214801343237	24.814916436197464
50	21.80186042671808	24.40359264861925	25.79927690540767	27.995270019255003
51	21.593770411396633	23.724932680330515	29.264449144208566	25.416847764064286
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2412.0
1	1923.5
2	1435.0
3	984.5
4	534.0
5	479.5
6	425.0
7	446.0
8	467.0
9	502.5
10	538.0
11	576.0
12	614.0
13	657.0
14	700.0
15	738.5
16	777.0
17	845.0
18	913.0
19	914.0
20	915.0
21	892.0
22	869.0
23	884.5
24	900.0
25	1071.5
26	1598.0
27	1953.0
28	2439.0
29	2925.0
30	3278.0
31	3631.0
32	4253.5
33	4876.0
34	5945.0
35	7014.0
36	8113.0
37	9212.0
38	9993.0
39	10774.0
40	13123.0
41	15472.0
42	19164.5
43	22857.0
44	31480.5
45	40104.0
46	42372.5
47	44641.0
48	43433.0
49	42225.0
50	39146.0
51	36067.0
52	33729.5
53	31392.0
54	27973.5
55	24555.0
56	22470.0
57	20385.0
58	18991.5
59	17598.0
60	16794.0
61	15990.0
62	14937.0
63	13884.0
64	12633.5
65	11383.0
66	9592.0
67	7801.0
68	6405.5
69	5010.0
70	4178.5
71	3347.0
72	2886.0
73	2425.0
74	1920.0
75	1349.5
76	1284.0
77	860.0
78	436.0
79	353.0
80	270.0
81	174.5
82	79.0
83	59.5
84	40.0
85	32.5
86	25.0
87	14.5
88	4.0
89	7.0
90	10.0
91	9.5
92	9.0
93	6.0
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	411841.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.976012403372614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.530182540417	24.635279208144453
2	8.446183517245878	5.232581707827393
3	2.8589826922173467	2.656796500054565
4	1.351140723041702	1.6741180718256647
5	0.7729419548544494	1.197132979032925
6	0.5390463532881375	1.0018503915267676
7	0.40672226413656537	0.8819043729035432
8	0.3519470137761695	0.8721532051248464
9	0.29328382522609603	0.8176287067120905
>10	4.461795716261653	34.15848669562996
>50	0.9009619606209902	17.978991431142468
>100	0.07820850352612761	3.946637418879753
>500	0.004692510211567657	1.047526867548711
>1k	0.0039104251763063804	3.8989124436468443
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCC	4262	1.0348653970828547	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGT	3420	0.8304175640599163	No Hit
AATCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCT	3231	0.7845260670987104	No Hit
CTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGCT	3070	0.7454333104280535	Illumina Single End Adapter 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2111	0.5125764554767496	No Hit
AAACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCT	925	0.22460124174135165	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	924	0.22435842958811775	No Hit
AATGACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTT	790	0.191821601054776	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCT	623	0.15127197146471574	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTT	552	0.1340323085851093	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	510	0.12383419814928577	No Hit
TGCGAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	469	0.11387889986669614	No Hit
AACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTG	455	0.11047952972142161	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	442	0.107322971729381	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTAT	425	0.1031951651244048	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4281215323389366E-4	0.0	0.0	1.0676450377694304	0.0
2	2.4281215323389366E-4	0.0	0.0	1.3099715666968563	0.0
3	2.4281215323389366E-4	0.0	0.0	1.7526181220422445	0.0
4	2.4281215323389366E-4	0.0	0.0	4.194579947115513	0.0
5	2.4281215323389366E-4	0.0	0.0	4.487168591762355	0.0
6	2.4281215323389366E-4	0.0	0.0	5.876539732566695	0.0
7	2.4281215323389366E-4	0.0	0.0	6.9376288421988095	0.0
8	2.4281215323389366E-4	0.0	0.0	7.504352407846717	0.0
9	2.4281215323389366E-4	0.0	0.0	9.432523716677068	0.0
10	2.4281215323389366E-4	0.0	0.0	10.790572089714235	0.0
11	2.4281215323389366E-4	0.0	0.0	13.370694029977589	0.0
12	2.4281215323389366E-4	0.0	0.0	14.106172042123053	0.0
13	2.4281215323389366E-4	0.0	0.0	14.429112205924131	0.0
14	2.4281215323389366E-4	0.0	0.0	14.851605352551106	0.0
15	2.4281215323389366E-4	0.0	0.0	15.142737124278545	0.0
16	2.4281215323389366E-4	0.0	0.0	15.658955762053802	0.0
17	2.4281215323389366E-4	0.0	0.0	16.30240796812362	0.0
18	2.4281215323389366E-4	0.0	0.0	17.174103598233298	0.0
19	2.4281215323389366E-4	0.0	0.0	17.531037463487124	0.0
20	2.4281215323389366E-4	0.0	0.0	17.88092977629716	0.0
21	2.4281215323389366E-4	0.0	0.0	18.263844541947012	0.0
22	2.4281215323389366E-4	0.0	0.0	18.672497395839656	0.0
23	2.4281215323389366E-4	0.0	0.0	19.103974592136286	0.0
24	2.4281215323389366E-4	0.0	0.0	19.400205419081637	0.0
25	0.001456872919403362	0.0	0.0	19.64787381538021	0.0
26	0.001456872919403362	0.0	0.0	19.8763600515733	0.0
27	0.001456872919403362	0.0	0.0	20.128399066630084	0.0
28	0.001456872919403362	0.0	0.0	20.37898120876746	0.0
29	0.001456872919403362	0.0	0.0	20.629806163058074	0.0
30	0.001456872919403362	0.0	0.0	20.98941096199747	0.0
31	0.001456872919403362	0.0	0.0	21.236593733989572	0.0
32	0.001456872919403362	0.0	0.0	21.50320147824039	0.0
33	0.001456872919403362	0.0	0.0	21.776122338475286	0.0
34	0.001456872919403362	0.0	0.0	22.008250756966888	0.0
35	0.001456872919403362	0.0	0.0	22.317350628033633	0.0
36	0.001456872919403362	0.0	0.0	22.564290587872506	0.0
37	0.001456872919403362	0.0	0.0	22.815601166469584	0.0
38	0.001456872919403362	0.0	0.0	23.080509225647763	0.0
39	0.001456872919403362	0.0	0.0	23.33667604730952	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCC	20	7.028007E-4	45.000004	13
CCTTCAA	20	7.028007E-4	45.000004	34
TTAGGGT	20	7.028007E-4	45.000004	4
ATGATCG	20	7.028007E-4	45.000004	27
AATCACG	20	7.028007E-4	45.000004	27
ACGCACG	20	7.028007E-4	45.000004	1
TTTCGCA	20	7.028007E-4	45.000004	13
CGTTGTA	20	7.028007E-4	45.000004	28
CGGTTAC	20	7.028007E-4	45.000004	37
TAAACCG	35	1.2093915E-7	45.000004	1
ACTTCGC	20	7.028007E-4	45.000004	12
GGTCATA	20	7.028007E-4	45.000004	8
CACTGGT	20	7.028007E-4	45.000004	28
CGACACG	35	1.2093915E-7	45.000004	1
ACGGGTC	20	7.028007E-4	45.000004	5
TGACCGG	20	7.028007E-4	45.000004	2
GTTAATA	20	7.028007E-4	45.000004	36
TAACACG	20	7.028007E-4	45.000004	1
TTGACGT	20	7.028007E-4	45.000004	33
CGGGCAA	45	3.8380676E-10	45.000004	6
>>END_MODULE