FastQCFastQC Report
Sat 18 Jun 2016
SRR3548820_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548820_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459939
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGCT43340.9422988700675525TruSeq Adapter, Index 23 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36890.8020628822517769No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCC30230.657261071576883No Hit
AATCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCT28410.6176906067978579No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGT25500.5544213471786477No Hit
AAACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCT17600.3826594396213411No Hit
AAAAACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTT11350.2467718545285353No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8790.19111229967452206No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG7590.16502188333670337No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCT6770.14719343217252723No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA6660.14480181067489384No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6560.14262760931340895No Hit
AATGACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTT6250.1358875850928058No Hit
TACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG5220.1134933110695114No Hit
AACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG5010.10892748821039312No Hit
AATGATCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCT4660.10131778344519599No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG351.2098099E-745.0000041
ACTATTG351.2098099E-745.00000414
TACCCAG351.2098099E-745.0000041
TTAATCG850.045.00000420
ATCGTAG351.2098099E-745.0000041
GTATCTC351.2098099E-745.0000049
ACCGTAG351.2098099E-745.0000041
CCCATAG351.2098099E-745.0000041
TCGCGGA351.2098099E-745.00000434
CCTTCGC207.0288696E-445.031
CTTCGTA207.0288696E-445.030
TTCTATC207.0288696E-445.011
TATCACT207.0288696E-445.023
AGGTATT253.8871432E-545.039
AGGTAAC253.8871432E-545.011
CCTTAGA207.0288696E-445.034
AATCCCG207.0288696E-445.01
GTACCCT207.0288696E-445.019
TGCCCTA207.0288696E-445.013
TCGCAAC253.8871432E-545.016