##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548820_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	459939
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.394917586897392	31.0	31.0	34.0	30.0	34.0
2	31.577139577204804	31.0	31.0	34.0	30.0	34.0
3	31.785865082108714	31.0	31.0	34.0	30.0	34.0
4	35.612418168496255	37.0	35.0	37.0	33.0	37.0
5	35.21171285757459	37.0	35.0	37.0	32.0	37.0
6	35.22327960881769	37.0	35.0	37.0	32.0	37.0
7	35.1953867795512	37.0	35.0	37.0	32.0	37.0
8	35.51704465157336	37.0	35.0	37.0	33.0	37.0
9	36.381870204527125	39.0	37.0	39.0	32.0	39.0
10	36.11374334422608	38.0	35.0	39.0	31.0	39.0
11	36.33753389036372	38.0	35.0	39.0	32.0	39.0
12	35.76124877429398	37.0	35.0	39.0	31.0	39.0
13	35.250683242777846	37.0	35.0	39.0	30.0	39.0
14	36.22546250698462	38.0	35.0	40.0	30.0	41.0
15	36.43404016619595	38.0	35.0	40.0	31.0	41.0
16	36.58229243443152	38.0	35.0	40.0	32.0	41.0
17	36.56816229978323	38.0	35.0	40.0	32.0	41.0
18	36.40680829414335	38.0	35.0	40.0	31.0	41.0
19	36.39906378889374	38.0	35.0	40.0	31.0	41.0
20	36.31030419251248	38.0	35.0	40.0	31.0	41.0
21	36.35380343915171	38.0	35.0	40.0	31.0	41.0
22	36.33677726828993	38.0	35.0	40.0	31.0	41.0
23	36.24540428187216	38.0	35.0	40.0	31.0	41.0
24	35.92133087213739	37.0	34.0	40.0	30.0	41.0
25	36.008585921176504	38.0	34.0	40.0	30.0	41.0
26	35.82171983676096	37.0	34.0	40.0	30.0	41.0
27	35.702432279063096	37.0	34.0	40.0	30.0	41.0
28	35.734445219909595	37.0	34.0	40.0	30.0	41.0
29	35.39390223486158	37.0	34.0	40.0	29.0	41.0
30	35.483494550364284	37.0	34.0	40.0	29.0	41.0
31	35.185157162145416	37.0	34.0	40.0	28.0	41.0
32	35.05074585977706	37.0	34.0	40.0	27.0	41.0
33	35.092383990050855	37.0	34.0	40.0	28.0	41.0
34	34.87591180569597	37.0	34.0	40.0	27.0	41.0
35	34.863277521584386	37.0	34.0	40.0	27.0	41.0
36	34.59370916578068	36.0	33.0	40.0	25.0	41.0
37	34.43557732655852	36.0	33.0	40.0	25.0	41.0
38	34.38170496522365	36.0	33.0	40.0	25.0	41.0
39	34.29805256784052	36.0	33.0	40.0	24.0	41.0
40	34.173162093234104	36.0	33.0	40.0	24.0	41.0
41	34.27540391225793	36.0	33.0	40.0	24.0	41.0
42	34.245628224612396	36.0	33.0	40.0	24.0	41.0
43	34.128030021372396	35.0	33.0	40.0	24.0	41.0
44	34.00242858292078	35.0	33.0	39.0	24.0	41.0
45	33.73247974187881	35.0	33.0	39.0	23.0	41.0
46	33.59961212247711	35.0	32.0	39.0	23.0	41.0
47	33.55393432607367	35.0	32.0	39.0	23.0	40.0
48	33.53729516305423	35.0	33.0	39.0	23.0	40.0
49	33.40448624708929	35.0	32.0	39.0	23.0	40.0
50	33.13205229389115	35.0	32.0	39.0	22.0	40.0
51	31.16971598407615	34.0	29.0	37.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	13.0
11	4.0
12	8.0
13	12.0
14	27.0
15	51.0
16	96.0
17	227.0
18	417.0
19	829.0
20	1301.0
21	1979.0
22	2714.0
23	3495.0
24	4239.0
25	5014.0
26	5791.0
27	6936.0
28	8371.0
29	10551.0
30	13184.0
31	17255.0
32	22440.0
33	31311.0
34	53920.0
35	58086.0
36	43362.0
37	55143.0
38	66092.0
39	47066.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.72935541452236	34.65720454234148	20.610776646468338	25.002663396667817
2	21.268037718045218	34.37107964317007	21.354570932232317	23.006311706552392
3	20.57229328237005	32.64258956078958	21.76832145132289	25.01679570551747
4	19.980693091910016	33.4246497905157	20.27334059516588	26.321316522408406
5	16.313467655493447	38.7784032230361	17.641469847088416	27.26665927438204
6	20.01721967478296	37.686084459026084	20.061138542284958	22.235557323905997
7	78.28038065917437	9.430163565168424	6.521299563637786	5.76815621201942
8	81.25273134046036	6.617181843679271	4.990661805152422	7.139425010707942
9	75.18453534055604	10.357243025705582	9.484953439477843	4.973268194260543
10	46.13285674839489	26.580263904561257	11.451083730668632	15.835795616375215
11	38.27855433003072	24.22255994816704	21.475456527930877	16.02342919387136
12	36.774006987883176	23.036098265204732	25.3648853434912	14.82500940342089
13	23.298306949399812	37.02382272431779	21.926385890302843	17.751484435979553
14	16.378258856065695	39.046047410634884	28.23004789765599	16.34564583564342
15	14.2475415218105	29.23974700992958	36.981425797768836	19.531285670491087
16	15.697298989648628	23.72618977734004	37.752397600551376	22.824113632459955
17	15.845362102365748	23.850554095216975	29.135602764714452	31.16848103770283
18	20.63904126416764	26.846168731070858	27.551914493008855	24.962875511752642
19	24.512163569516826	30.182480720269428	25.796246893609805	19.50910881660394
20	25.703843335746697	30.47665016447833	22.937172103257172	20.8823343965178
21	20.527504734323465	31.242621304129457	24.715451396815666	23.51442256473141
22	18.190238270727203	28.943620784495334	24.739150191655852	28.12699075312161
23	20.135713648983888	31.440473628024584	21.101493893755478	27.322318829236053
24	16.924853078343	30.866919308864873	29.469777513974677	22.738450098817452
25	19.04991748905833	27.15251370290408	28.869697938204848	24.927870869832738
26	20.417707565568477	34.372384163986965	22.49820084837337	22.711707422071186
27	16.89137037737613	31.291105994490575	26.15520753839096	25.662316089742337
28	21.331524397800578	28.861870813303504	30.434905498337823	19.371699290558094
29	17.395350252968328	31.86879129623711	28.747942661961694	21.98791578883287
30	22.352964197426182	27.561698399135537	26.30348807124423	23.781849332194053
31	29.813083908953143	30.59210025677318	18.41592037204934	21.17889546222434
32	27.060110145040973	28.76555369298972	20.986478641732926	23.18785752023638
33	27.216652643067885	26.836602245080325	23.528337453444912	22.418407658406874
34	20.09396898284338	31.1073859794451	22.88825257262376	25.91039246508776
35	21.784845381670177	26.82725317922594	26.325447504995225	25.062453934108653
36	28.26961836243502	27.183169942101017	25.53099432750865	19.016217367955317
37	21.083013182182857	32.40407967143469	21.565033624024053	24.9478735223584
38	23.916649816606114	31.098254333726867	22.448411637195367	22.536684212471652
39	21.54111740904772	30.24857644165857	25.443374012640806	22.766932136652905
40	23.201554988813733	26.9557484796897	22.660613690076293	27.182082841420275
41	19.10514220364005	23.55290592882969	28.1709096206236	29.17104224690665
42	25.07419462146067	24.499335781484067	21.892033508791382	28.534436088263877
43	21.157805709017936	25.40054224581955	25.440112710598576	28.00153933456393
44	20.863418844672882	27.030106166252484	24.82263952393687	27.283835465137766
45	23.77467446770115	30.607319666303574	23.340921296084915	22.277084569910357
46	20.11071033332681	28.946229826129116	29.720897771226184	21.22216206931789
47	21.48110945147074	26.209562572428087	26.20238770793518	26.106940268165996
48	22.898906159295038	22.19903074103305	31.8055220366179	23.096541063054012
49	21.80745707582962	21.287605530298585	29.80221290214572	27.102724491726075
50	20.293125827555393	25.242260386703453	26.33914497357258	28.12546881216857
51	19.390180002130716	26.386325143116807	29.77025214213189	24.453242712620586
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4184.0
1	3286.5
2	2389.0
3	1650.5
4	912.0
5	836.0
6	760.0
7	797.0
8	834.0
9	924.5
10	1015.0
11	1026.5
12	1038.0
13	1110.0
14	1182.0
15	1248.0
16	1314.0
17	1274.5
18	1235.0
19	1245.5
20	1256.0
21	1192.0
22	1128.0
23	1302.0
24	1476.0
25	1602.0
26	1990.0
27	2252.0
28	2650.5
29	3049.0
30	4093.5
31	5138.0
32	5703.0
33	6268.0
34	6762.0
35	7256.0
36	7712.5
37	8169.0
38	8968.5
39	9768.0
40	13661.0
41	17554.0
42	25674.5
43	33795.0
44	42280.0
45	50765.0
46	50732.0
47	50699.0
48	51471.0
49	52243.0
50	47416.0
51	42589.0
52	37772.5
53	32956.0
54	29301.0
55	25646.0
56	23007.5
57	20369.0
58	18836.0
59	17303.0
60	16138.5
61	14974.0
62	13580.5
63	12187.0
64	10756.5
65	9326.0
66	7517.5
67	5709.0
68	4919.5
69	4130.0
70	3572.5
71	3015.0
72	2303.0
73	1591.0
74	1420.5
75	1046.5
76	843.0
77	584.0
78	325.0
79	266.0
80	207.0
81	132.0
82	57.0
83	41.5
84	26.0
85	18.0
86	10.0
87	10.0
88	10.0
89	7.0
90	4.0
91	3.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	459939.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.560132520726548
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.64647321940845	21.95067356735395
2	9.20687223529852	5.074852378124501
3	3.3091974311487458	2.736057592191219
4	1.5452605739435734	1.7035034478775541
5	0.8884610615396219	1.2243052297768682
6	0.534520421765298	0.8838872195331757
7	0.37597497950598374	0.7253344181763652
8	0.2640347926843441	0.5821467101170468
9	0.22147078093158953	0.5493387664749065
>10	2.2903593116348766	15.408561541214159
>50	1.118382467941844	22.522560075397298
>100	0.5871704993775009	21.29160424688024
>500	0.006305186570496654	1.1479798047843022
>1k	0.0055170382491845716	4.199195002098416
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGCT	4334	0.9422988700675525	TruSeq Adapter, Index 23 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3689	0.8020628822517769	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCC	3023	0.657261071576883	No Hit
AATCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCT	2841	0.6176906067978579	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGT	2550	0.5544213471786477	No Hit
AAACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCT	1760	0.3826594396213411	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTT	1135	0.2467718545285353	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	879	0.19111229967452206	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	759	0.16502188333670337	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCT	677	0.14719343217252723	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	666	0.14480181067489384	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	656	0.14262760931340895	No Hit
AATGACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTT	625	0.1358875850928058	No Hit
TACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	522	0.1134933110695114	No Hit
AACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTG	501	0.10892748821039312	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCT	466	0.10131778344519599	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.3093040598862022	0.0
2	0.0	0.0	0.0	1.6049954450481476	0.0
3	0.0	0.0	0.0	2.034400213941414	0.0
4	0.0	0.0	0.0	4.596913938587508	0.0
5	0.0	0.0	0.0	4.944786156425091	0.0
6	0.0	0.0	0.0	6.587612705163076	0.0
7	0.0	0.0	0.0	7.946488556091134	0.0
8	0.0	0.0	0.0	8.776816056042215	0.0
9	0.0	0.0	0.0	10.52030812781695	0.0
10	0.0	0.0	0.0	12.279454449394375	0.0
11	0.0	0.0	0.0	14.628896440614952	0.0
12	0.0	0.0	0.0	15.454875537843062	0.0
13	0.0	0.0	0.0	15.785354144788766	0.0
14	0.0	0.0	0.0	16.177145230128342	0.0
15	0.0	0.0	0.0	16.467618532022726	0.0
16	0.0	0.0	0.0	17.055087739895942	0.0
17	0.0	0.0	0.0	17.718653995421132	0.0
18	0.0	0.0	0.0	18.72487438551634	0.0
19	0.0	0.0	0.0	19.06709367981406	0.0
20	0.0	0.0	0.0	19.432142088407375	0.0
21	0.0	0.0	0.0	19.784797549240224	0.0
22	0.0	0.0	0.0	20.156585982054143	0.0
23	0.0	0.0	0.0	20.563161636651817	0.0
24	0.0	0.0	0.0	20.846677494189446	0.0
25	0.0	0.0	0.0	21.08257834191056	0.0
26	0.0	0.0	0.0	21.30499914119046	0.0
27	0.0	0.0	0.0	21.529811561968	0.0
28	0.0	0.0	0.0	21.73527359062832	0.0
29	0.0	0.0	0.0	21.964651834264977	0.0
30	0.0	0.0	0.0	22.326873781088363	0.0
31	0.0	0.0	0.0	22.581255340382093	0.0
32	0.0	0.0	0.0	22.827374934502185	0.0
33	0.0	0.0	0.0	23.068711285627007	0.0
34	0.0	0.0	0.0	23.28721852245624	0.0
35	2.1742013614848925E-4	0.0	0.0	23.60465192123303	0.0
36	2.1742013614848925E-4	0.0	0.0	23.807722328395723	0.0
37	2.1742013614848925E-4	0.0	0.0	24.022750843046577	0.0
38	2.1742013614848925E-4	0.0	0.0	24.249085204777156	0.0
39	2.1742013614848925E-4	0.0	0.0	24.485638312906712	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	35	1.2098099E-7	45.000004	1
ACTATTG	35	1.2098099E-7	45.000004	14
TACCCAG	35	1.2098099E-7	45.000004	1
TTAATCG	85	0.0	45.000004	20
ATCGTAG	35	1.2098099E-7	45.000004	1
GTATCTC	35	1.2098099E-7	45.000004	9
ACCGTAG	35	1.2098099E-7	45.000004	1
CCCATAG	35	1.2098099E-7	45.000004	1
TCGCGGA	35	1.2098099E-7	45.000004	34
CCTTCGC	20	7.0288696E-4	45.0	31
CTTCGTA	20	7.0288696E-4	45.0	30
TTCTATC	20	7.0288696E-4	45.0	11
TATCACT	20	7.0288696E-4	45.0	23
AGGTATT	25	3.8871432E-5	45.0	39
AGGTAAC	25	3.8871432E-5	45.0	11
CCTTAGA	20	7.0288696E-4	45.0	34
AATCCCG	20	7.0288696E-4	45.0	1
GTACCCT	20	7.0288696E-4	45.0	19
TGCCCTA	20	7.0288696E-4	45.0	13
TCGCAAC	25	3.8871432E-5	45.0	16
>>END_MODULE