##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548819_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	470364
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19166220203927	31.0	31.0	34.0	28.0	34.0
2	31.404833278056994	31.0	31.0	34.0	28.0	34.0
3	31.408704747812333	31.0	31.0	34.0	28.0	34.0
4	35.4158311435399	37.0	35.0	37.0	33.0	37.0
5	35.06750304019865	37.0	35.0	37.0	32.0	37.0
6	35.131564065277104	37.0	35.0	37.0	32.0	37.0
7	35.06123980576745	37.0	35.0	37.0	32.0	37.0
8	35.44346506110161	37.0	35.0	37.0	33.0	37.0
9	35.87650202821644	39.0	37.0	39.0	32.0	39.0
10	35.78138633058652	38.0	35.0	39.0	30.0	39.0
11	36.15897262545603	38.0	35.0	39.0	32.0	39.0
12	35.64765585801634	37.0	35.0	39.0	31.0	39.0
13	35.13053932698931	37.0	34.0	39.0	30.0	39.0
14	36.09892763901999	38.0	35.0	40.0	30.0	41.0
15	36.33933719417303	38.0	35.0	40.0	31.0	41.0
16	36.49774642617207	38.0	35.0	40.0	31.0	41.0
17	36.4787143573913	38.0	35.0	40.0	31.0	41.0
18	36.35039458802119	38.0	35.0	40.0	31.0	41.0
19	36.343395753076344	38.0	35.0	40.0	31.0	41.0
20	36.22604195899346	38.0	34.0	40.0	31.0	41.0
21	36.2563100067182	38.0	34.0	40.0	31.0	41.0
22	36.26299206571932	38.0	35.0	40.0	31.0	41.0
23	36.18535432133412	38.0	35.0	40.0	31.0	41.0
24	35.816070107406176	37.0	34.0	40.0	30.0	41.0
25	35.937722699866484	37.0	34.0	40.0	30.0	41.0
26	35.75543196333053	37.0	34.0	40.0	30.0	41.0
27	35.635333486406275	37.0	34.0	40.0	30.0	41.0
28	35.62993128725838	37.0	34.0	40.0	29.0	41.0
29	35.316129635771446	37.0	34.0	40.0	29.0	41.0
30	35.432643654701465	37.0	34.0	40.0	29.0	41.0
31	35.18897917357621	37.0	34.0	40.0	28.0	41.0
32	35.05882678096113	37.0	34.0	40.0	27.0	41.0
33	35.06518355996632	37.0	34.0	40.0	28.0	41.0
34	34.92633790000936	37.0	34.0	40.0	27.0	41.0
35	34.87697612912553	37.0	34.0	40.0	27.0	41.0
36	34.66229983587179	36.0	33.0	40.0	26.0	41.0
37	34.47761946067301	36.0	33.0	40.0	25.0	41.0
38	34.45705028446054	36.0	33.0	40.0	26.0	41.0
39	34.30411128402684	36.0	33.0	40.0	24.0	41.0
40	34.145338503797056	36.0	33.0	40.0	24.0	41.0
41	34.24298203093774	36.0	33.0	40.0	24.0	41.0
42	34.26124023097006	36.0	33.0	40.0	25.0	41.0
43	34.174573309181824	35.0	33.0	40.0	25.0	41.0
44	34.14274901990799	35.0	33.0	39.0	25.0	41.0
45	33.89768137017289	35.0	33.0	39.0	23.0	41.0
46	33.680362442703945	35.0	33.0	39.0	23.0	41.0
47	33.602784226684015	35.0	32.0	39.0	23.0	40.0
48	33.54050054851137	35.0	33.0	39.0	23.0	40.0
49	33.41340323664226	35.0	32.0	39.0	23.0	40.0
50	33.23719714944171	35.0	32.0	39.0	23.0	40.0
51	31.42809186077166	34.0	29.0	37.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	8.0
10	10.0
11	8.0
12	7.0
13	8.0
14	18.0
15	40.0
16	99.0
17	220.0
18	448.0
19	798.0
20	1433.0
21	1987.0
22	2736.0
23	3493.0
24	4254.0
25	4983.0
26	5844.0
27	7116.0
28	8871.0
29	11049.0
30	14043.0
31	18073.0
32	23791.0
33	33233.0
34	56590.0
35	58801.0
36	44651.0
37	57018.0
38	66087.0
39	44641.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.012585997227678	34.27515711236404	20.652090721228667	25.060166169179617
2	21.59582791200007	33.93775884208826	21.360478267894653	23.105934978017025
3	20.93229924058814	32.45932937044502	21.48485003103979	25.123521357927054
4	19.83506390795214	33.51298143565409	20.259416111777263	26.39253854461651
5	16.50997950523424	38.769973892559804	17.43415737598966	27.28588922621629
6	19.980695801549437	37.635320730328004	20.075941185975115	22.308042282147444
7	78.6607818625575	9.410371542039782	6.1879735694058216	5.740873025996888
8	81.84937622777252	6.612113171926423	4.80308867175209	6.735421928548954
9	74.9468496738696	11.320169060557356	8.959231573844937	4.773749691728108
10	46.84372103307226	26.51861111819782	11.318893452730226	15.318774395999693
11	38.983638203604016	24.22613125154136	20.91507853492189	15.875152009932734
12	37.2928200287437	23.11188781454363	24.848415269876096	14.746876886836576
13	23.355316308220868	37.35596261618661	21.64876563682595	17.63995543876657
14	16.560578615710387	39.564039764948	27.70535160003742	16.1700300193042
15	14.287658069069911	29.286892704373635	37.16738525907595	19.258063967480503
16	15.923412506059137	23.757983178984787	37.85047325050386	22.468131064452212
17	15.907254806915494	23.517743704875375	29.055369883749606	31.519631604459526
18	20.899771240996333	26.748220527081155	27.713005247000194	24.639002984922314
19	25.194104991028226	29.742072097354388	25.63185107703821	19.431971834579176
20	26.138479985713193	30.329914704356625	22.59632965107874	20.93527565885144
21	20.6780280803803	31.317872966468524	24.604561573589816	23.399537379561362
22	18.3251694432397	28.871894957947465	24.648782644930307	28.154152953882527
23	20.05744487248174	31.5479075779609	21.17211351208851	27.222534037468854
24	16.98790723779881	30.675604425508755	29.876436121812045	22.460052214880392
25	18.835625175396075	27.27738517403543	29.242246430424096	24.6447432201444
26	20.25920351047274	34.55366482128734	22.53531307668104	22.65181859155888
27	16.934119107754846	31.612538374535465	26.170370181391434	25.282972336318256
28	20.89955863969181	29.323672730055872	30.73853441164715	19.038234218605165
29	17.262588123240725	31.659523262834742	29.202064783869513	21.875823830055023
30	22.23745864904627	27.88308629061748	26.620447143063668	23.25900791727258
31	29.791608201307923	30.558886309326393	18.578802799533978	21.070702689831705
32	27.416213825888036	28.67119932647907	21.174239525133725	22.738347322499173
33	27.24868399792501	27.189368233963485	23.389119915639803	22.172827852471702
34	20.153753263430023	31.10420865542431	22.932239712222874	25.80979836892279
35	21.637497767686302	26.929144237229043	26.60769106479237	24.825666930292282
36	28.71371958738339	27.226998664863807	25.26149960456157	18.79778214319123
37	21.14064851901931	32.908130724290125	21.70574278643774	24.24547797025283
38	23.564941194479168	31.399086664795778	22.317821942155437	22.718150198569617
39	21.638985976817953	30.56250053150326	25.17305746188059	22.6254560297982
40	23.46395557483141	26.9002304598141	22.551045573215635	27.08476839213885
41	19.31397811056969	23.610225272342273	27.830361167096125	29.24543544999192
42	24.879029857727204	24.760823532413195	21.715522446445732	28.644624163413866
43	21.159570035121735	25.674583939247054	25.382894949443408	27.782951076187807
44	20.81813234006004	27.397292309785616	24.87945506033625	26.905120289818097
45	23.286433485555868	31.206044680290155	23.313859053839153	22.19366278031482
46	20.270471379612385	29.277538246974682	29.28646750176459	21.16552287164834
47	21.3747225552976	26.498413994268265	26.223095304912793	25.90376814552134
48	22.851238615200142	22.45346157444022	31.55407301579202	23.14122679456761
49	21.920257502700036	21.486338240171442	29.89110561182403	26.702298645304488
50	20.466277181076784	25.733474500599534	26.362349159374443	27.437899158949243
51	19.442389298500736	26.967837674651975	29.387878324021393	24.201894702825896
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4417.0
1	3513.0
2	2609.0
3	1754.5
4	900.0
5	821.5
6	743.0
7	786.5
8	830.0
9	897.5
10	965.0
11	990.5
12	1016.0
13	1072.5
14	1129.0
15	1202.0
16	1275.0
17	1244.5
18	1214.0
19	1223.5
20	1233.0
21	1206.0
22	1179.0
23	1369.0
24	1559.0
25	1678.0
26	2037.0
27	2277.0
28	2763.0
29	3249.0
30	4413.5
31	5578.0
32	6119.0
33	6660.0
34	7085.5
35	7511.0
36	8095.0
37	8679.0
38	9446.0
39	10213.0
40	14270.5
41	18328.0
42	26884.0
43	35440.0
44	43704.0
45	51968.0
46	51246.0
47	50524.0
48	51558.0
49	52592.0
50	47957.0
51	43322.0
52	38268.5
53	33215.0
54	29795.5
55	26376.0
56	23563.5
57	20751.0
58	19233.5
59	17716.0
60	16620.5
61	15525.0
62	13919.0
63	12313.0
64	11011.0
65	9709.0
66	7840.0
67	5971.0
68	5097.0
69	4223.0
70	3597.5
71	2972.0
72	2280.0
73	1588.0
74	1438.0
75	1063.5
76	839.0
77	588.5
78	338.0
79	278.0
80	218.0
81	135.0
82	52.0
83	39.0
84	26.0
85	20.5
86	15.0
87	12.0
88	9.0
89	6.5
90	4.0
91	2.0
92	0.0
93	1.5
94	3.0
95	2.5
96	2.0
97	2.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	470364.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.955678911854992
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.79562153679397	22.30740774254513
2	9.107926270160302	5.092365247229008
3	3.3234149082340925	2.787249601963929
4	1.5785133507084743	1.765136495619298
5	0.9411599097267331	1.3155382120515489
6	0.5562715291947277	0.9330568954784626
7	0.37804594007211795	0.7397971640210552
8	0.2479438564266193	0.5545151070743715
9	0.21523208596476748	0.541526317808383
>10	2.1895568592206387	15.004979283212972
>50	1.0502872175171678	21.525099168345243
>100	0.6061396903411894	22.563488219709285
>500	0.0045631595257805985	0.9108228288377455
>1k	0.005323686113410698	3.959017716103561
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCTGCT	4242	0.9018547337806465	TruSeq Adapter, Index 23 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3889	0.8268064732845202	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCC	2855	0.6069767244091809	No Hit
AATCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCT	2486	0.528526843040709	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGT	2484	0.5281016404316657	No Hit
AAACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTTCT	1642	0.34909134202447467	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTT	1023	0.21749113452560145	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	952	0.20239644190456754	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	788	0.16752982796302437	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	703	0.149458717078688	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	648	0.13776564532999974	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCT	619	0.13160020749887322	No Hit
AATGACTGTCTCTTATACACATCTGACGCGACGATACTCGTATGCCGTCTT	574	0.12203314879540102	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1260130452160455E-4	0.0	0.0	1.2277725336122662	0.0
2	2.1260130452160455E-4	0.0	0.0	1.5022408177496578	0.0
3	2.1260130452160455E-4	0.0	0.0	1.8955532311146261	0.0
4	2.1260130452160455E-4	0.0	0.0	4.156355503397369	0.0
5	2.1260130452160455E-4	0.0	0.0	4.472493643220995	0.0
6	2.1260130452160455E-4	0.0	0.0	5.987915741850992	0.0
7	2.1260130452160455E-4	0.0	0.0	7.24247603983298	0.0
8	2.1260130452160455E-4	0.0	0.0	8.00422651393389	0.0
9	2.1260130452160455E-4	0.0	0.0	9.615531800903131	0.0
10	2.1260130452160455E-4	0.0	0.0	11.2015375326343	0.0
11	2.1260130452160455E-4	0.0	0.0	13.353700538306503	0.0
12	2.1260130452160455E-4	0.0	0.0	14.141813574168092	0.0
13	4.252026090432091E-4	0.0	0.0	14.444557831806856	0.0
14	4.252026090432091E-4	0.0	0.0	14.827027578641223	0.0
15	4.252026090432091E-4	0.0	0.0	15.104259679737394	0.0
16	4.252026090432091E-4	0.0	0.0	15.643841790613227	0.0
17	4.252026090432091E-4	0.0	0.0	16.29971681506238	0.0
18	4.252026090432091E-4	0.0	0.0	17.248556437142298	0.0
19	4.252026090432091E-4	0.0	0.0	17.57638764871461	0.0
20	6.378039135648136E-4	0.0	0.0	17.913148115076833	0.0
21	6.378039135648136E-4	0.0	0.0	18.275420737981648	0.0
22	6.378039135648136E-4	0.0	0.0	18.62536248522421	0.0
23	6.378039135648136E-4	0.0	0.0	19.031643578164996	0.0
24	6.378039135648136E-4	0.0	0.0	19.28931635924518	0.0
25	6.378039135648136E-4	0.0	0.0	19.51552414725617	0.0
26	6.378039135648136E-4	0.0	0.0	19.722597817860212	0.0
27	6.378039135648136E-4	0.0	0.0	19.941789762821983	0.0
28	6.378039135648136E-4	0.0	0.0	20.165658936483233	0.0
29	6.378039135648136E-4	0.0	0.0	20.396543953193696	0.0
30	6.378039135648136E-4	0.0	0.0	20.742233674345826	0.0
31	6.378039135648136E-4	0.0	0.0	20.982047945846197	0.0
32	6.378039135648136E-4	0.0	0.0	21.227389851264128	0.0
33	6.378039135648136E-4	0.0	0.0	21.461889090151455	0.0
34	6.378039135648136E-4	0.0	0.0	21.67427779336854	0.0
35	6.378039135648136E-4	0.0	0.0	21.972770024916873	0.0
36	6.378039135648136E-4	0.0	0.0	22.164323800290838	0.0
37	6.378039135648136E-4	0.0	0.0	22.375649496985314	0.0
38	6.378039135648136E-4	0.0	0.0	22.598880866732998	0.0
39	6.378039135648136E-4	0.0	0.0	22.831466693879634	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGT	20	7.0290314E-4	45.000004	28
CTTAACG	20	7.0290314E-4	45.000004	1
TTGATCT	20	7.0290314E-4	45.000004	40
TATCAGT	30	2.1627566E-6	45.000004	25
AGACTTA	30	2.1627566E-6	45.000004	24
CTAGACC	20	7.0290314E-4	45.000004	38
CGTGGGA	20	7.0290314E-4	45.000004	4
TAATACA	20	7.0290314E-4	45.000004	8
AACCGGC	20	7.0290314E-4	45.000004	28
ACTATCC	30	2.1627566E-6	45.000004	16
GTACCTA	20	7.0290314E-4	45.000004	22
CCTTACA	30	2.1627566E-6	45.000004	20
TACCCGT	35	1.2098826E-7	45.000004	35
ACCGGCC	20	7.0290314E-4	45.000004	10
TCCGCTA	20	7.0290314E-4	45.000004	31
ATTAGCG	20	7.0290314E-4	45.000004	19
GGTCGTA	20	7.0290314E-4	45.000004	9
CTACTAA	20	7.0290314E-4	45.000004	30
GATAACT	20	7.0290314E-4	45.000004	10
GTCGAGG	30	2.1627566E-6	45.000004	2
>>END_MODULE