SRR3548818_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548818_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	442598
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCC	22070	4.986466274135898	No Hit
AATCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCT	19420	4.387728819380115	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGT	18734	4.232734897130127	No Hit
CTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGCT	7338	1.6579379030180887	Illumina Single End Adapter 2 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTT	4778	1.079534927857785	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCT	3713	0.838910252644612	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2770	0.6258500942164221	No Hit
AACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTG	2490	0.5625872688082639	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTAT	2258	0.5101694991843615	No Hit
AAACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCT	1592	0.3596943501778137	No Hit
AATGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTC	1375	0.31066566048649114	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTT	1212	0.2738376585524562	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGG	836	0.18888472157578662	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATG	784	0.17713591114284294	No Hit
AATCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTTT	764	0.17261713789940306	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCT	701	0.15838300218256748	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	694	0.15680143154736353	No Hit
AATGATACGGCCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGC	655	0.14798982372265576	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCTTGGTTC	651	0.1470860690739678	No Hit
AACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTT	648	0.1464082530874518	No Hit
CCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGC	630	0.14234135716835594	No Hit
AATGATACCTGTCTCTTCTACACATCTGACGCTCCTTGGTTCGTATGCCGT	587	0.1326259946949602	No Hit
AATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTA	554	0.12517001884328444	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	492	0.11116182178862082	No Hit
TACCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCT	461	0.10415772326128903	No Hit
CTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTTCT	461	0.10415772326128903	No Hit
AATCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCC	454	0.10257615262608506	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCG	30	2.1624655E-6	45.000004	1
TTCTACG	30	2.1624655E-6	45.000004	1
GCGACGT	25	3.886904E-5	45.000004	28
CATATAG	25	3.886904E-5	45.000004	1
ATCTACG	30	2.1624655E-6	45.000004	1
ACGCATT	25	3.886904E-5	45.000004	14
ACGCATG	30	2.1624655E-6	45.000004	1
GGACTAT	50	2.1827873E-11	45.000004	8
GTCTCGC	65	0.0	45.000004	31
GGATCTA	50	2.1827873E-11	45.000004	8
GCAGTAC	65	0.0	45.000004	38
TGAACGG	25	3.886904E-5	45.000004	2
ATGCTAG	30	2.1624655E-6	45.000004	1
ATAGAGG	25	3.886904E-5	45.000004	2
ATCACCG	25	3.886904E-5	45.000004	1
ACGATAT	30	2.1624655E-6	45.000004	24
CCGTAGG	30	2.1624655E-6	45.000004	2
GAGACGG	30	2.1624655E-6	45.000004	2
TCTACGC	25	3.886904E-5	45.000004	11
TACGATA	30	2.1624655E-6	45.000004	23

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content