##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548818_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	442598
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.08943104126092	31.0	31.0	33.0	30.0	34.0
2	31.25046204456414	31.0	31.0	34.0	30.0	34.0
3	31.35863921662547	31.0	31.0	34.0	28.0	34.0
4	35.158572790658795	35.0	35.0	37.0	32.0	37.0
5	34.65431384687685	35.0	35.0	37.0	32.0	37.0
6	34.634196268397055	35.0	35.0	37.0	31.0	37.0
7	34.45600748308849	35.0	35.0	37.0	30.0	37.0
8	34.91037465148961	35.0	35.0	37.0	32.0	37.0
9	35.99675326142459	38.0	35.0	39.0	32.0	39.0
10	35.67112820211569	37.0	35.0	39.0	30.0	39.0
11	35.98113864048188	37.0	35.0	39.0	31.0	39.0
12	35.380593676428724	37.0	35.0	39.0	30.0	39.0
13	35.357977216345304	37.0	35.0	39.0	30.0	39.0
14	36.161611665665006	38.0	34.0	40.0	30.0	41.0
15	36.32720888933073	38.0	35.0	40.0	31.0	41.0
16	36.13924825688322	38.0	34.0	40.0	30.0	41.0
17	36.07179426929177	38.0	34.0	40.0	30.0	41.0
18	35.67207488511019	37.0	34.0	40.0	29.0	41.0
19	35.66963700694536	37.0	34.0	40.0	29.0	41.0
20	35.88415221035793	37.0	34.0	40.0	30.0	41.0
21	36.1983108825616	38.0	34.0	40.0	31.0	41.0
22	36.14768480652872	38.0	34.0	40.0	30.0	41.0
23	36.10807549966335	38.0	34.0	40.0	30.0	41.0
24	35.68294705353391	37.0	34.0	40.0	29.0	41.0
25	35.74408379613103	37.0	34.0	40.0	30.0	41.0
26	35.69900451425447	37.0	34.0	40.0	30.0	41.0
27	35.766765778426475	38.0	34.0	40.0	30.0	41.0
28	35.6237985711639	37.0	34.0	40.0	29.0	41.0
29	35.45631701905567	37.0	34.0	40.0	29.0	41.0
30	35.51332360290828	37.0	34.0	40.0	29.0	41.0
31	35.056227095468124	37.0	34.0	40.0	27.0	41.0
32	35.13367209070082	37.0	34.0	40.0	27.0	41.0
33	34.762784739199	37.0	34.0	40.0	26.0	41.0
34	34.85977342870957	37.0	34.0	40.0	26.0	41.0
35	34.33220891192459	37.0	33.0	40.0	25.0	41.0
36	34.404276566997595	37.0	33.0	40.0	25.0	41.0
37	34.27632750260959	37.0	33.0	40.0	24.0	41.0
38	33.702750577273285	36.0	32.0	39.0	23.0	40.0
39	33.71461461642393	36.0	33.0	39.0	23.0	40.0
40	32.81159652777464	35.0	30.0	39.0	18.0	40.0
41	33.47483043303404	36.0	32.0	39.0	23.0	40.0
42	32.96216883040592	35.0	31.0	39.0	18.0	40.0
43	33.20239359418705	35.0	31.0	39.0	21.0	40.0
44	33.13019715407661	35.0	31.0	39.0	21.0	40.0
45	32.36288234470106	35.0	30.0	39.0	15.0	40.0
46	32.44948237452496	35.0	30.0	39.0	15.0	40.0
47	32.102248541565935	35.0	30.0	39.0	15.0	40.0
48	32.179280069046854	35.0	30.0	39.0	14.0	40.0
49	32.10049299816086	35.0	30.0	39.0	14.0	40.0
50	31.54993018495339	35.0	29.0	39.0	12.0	40.0
51	29.234198979661002	33.0	23.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	4.0
12	7.0
13	5.0
14	5.0
15	22.0
16	58.0
17	138.0
18	285.0
19	657.0
20	1122.0
21	1814.0
22	2706.0
23	3867.0
24	4939.0
25	6134.0
26	7433.0
27	9133.0
28	11285.0
29	13659.0
30	16956.0
31	21027.0
32	26170.0
33	33279.0
34	48343.0
35	51755.0
36	44555.0
37	52145.0
38	54474.0
39	30619.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.121392324411769	49.788069534882666	15.439066602198833	20.651471538506726
2	14.54096042006516	50.46498176674996	17.851639636871383	17.142418176313495
3	15.313670644693378	26.177027460585002	38.56162928888065	19.947672605840967
4	30.505108473151708	25.824337208934516	17.0520427114447	26.618511606469074
5	12.52242441222057	44.6682994500653	20.862046371651026	21.94722976606311
6	21.251112747911197	28.672745922936844	32.50963628394163	17.566505045210327
7	56.90445957731395	20.576685841327798	14.087953402410314	8.43090117894794
8	60.08115716745218	5.816113041631458	8.605325826144718	25.497403964771642
9	61.56580011658435	9.589062761241578	17.983361876917655	10.861775245256418
10	35.40029552777012	27.408167230760196	17.670662768471615	19.52087447299807
11	28.637273552975838	18.146715529668008	31.68586392166255	21.53014699569361
12	24.911770952421836	19.62480625759719	42.06955295776303	13.39386983221795
13	22.395943949136687	34.98140524810324	21.597702655683033	21.024948147077033
14	10.54252391560739	31.144740825760625	44.29414502550848	14.018590233123513
15	8.255572777102472	30.861865620721286	31.591647499536823	29.290914102639416
16	9.810482650170131	24.536712773216326	41.490698105278376	24.16210647133516
17	9.28336775132287	28.164609871712027	31.40773342852882	31.144288948436277
18	12.121609225527454	28.67048653631512	29.506007709027156	29.701896529130273
19	14.461204072318449	32.56363562420074	36.50219838318293	16.472961920297877
20	17.557241560061275	35.967401569821824	27.059543875028808	19.415812995088093
21	12.186227682908644	28.35823930519343	28.407042056222576	31.04849095567535
22	12.722606066904957	36.017785891486184	29.41947320141528	21.840134840193585
23	20.197786704865365	26.96216431163268	19.51838914771418	33.321659835787784
24	10.223498524620537	42.83480720653957	25.03332595267037	21.908368316169526
25	13.326540110890694	23.988811517449243	31.559338270846233	31.12531010081383
26	20.479306277931666	33.560477001703575	19.314366535772866	26.645850184591886
27	10.44717780017081	26.576938892629432	32.85215929579437	30.123724011405383
28	18.678349201758706	21.434574941594857	35.10363806433829	24.783437792308145
29	12.421881707554034	28.330674788408444	30.978449970402032	28.268993533635488
30	21.06652086091668	21.490155852489163	24.698936732655817	32.744386553938334
31	24.94295048780157	27.513906524656683	27.017519283864814	20.525623703676928
32	18.263073940686585	20.020876732384693	28.52814517914676	33.18790414778196
33	30.703482618538718	19.406097632614696	30.09254447602565	19.797875272820935
34	20.627522040316496	19.02539098685489	26.77779836330033	33.569288609528286
35	17.69054537074275	17.064243399201985	38.905733871368604	26.339477358686665
36	22.32138419061993	16.216521538732664	37.05891124677473	24.40318302387268
37	16.440652691607283	21.10402667883723	31.076959227109025	31.378361402446465
38	27.998770893677783	19.621417177664608	36.00671489703976	16.373097031617856
39	23.55207208347078	19.110795801155902	37.48367593165807	19.853456183715245
40	26.64991708051098	24.146064826320952	27.52000686853533	21.684011224632737
41	23.84443671232134	15.920767829949526	38.70735972598159	21.527435731747545
42	25.552758937003784	19.322952204935405	29.815769614864955	25.30851924319586
43	21.180619885313533	18.73664137659908	31.747093299111157	28.335645438976226
44	18.949023719040756	17.564923474575124	28.30672529021821	35.17932751616591
45	29.095929037184987	26.104501150027794	24.44972638828011	20.349843424507114
46	15.788593712578908	18.82633902548136	45.082670956488734	20.302396305450994
47	24.118952186860312	23.216101292821026	23.52947821725358	29.135468303065082
48	18.492401682791158	15.23346241962232	42.728841973980906	23.54529392360562
49	22.590477137266777	13.973854378013456	35.577883316237305	27.85778516848246
50	19.36181365482899	17.48448931084189	26.057505908296015	37.096191126033105
51	14.955105987826425	17.391854459351375	40.49973113299202	27.153308419830186
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3271.0
1	2637.5
2	2004.0
3	1409.5
4	815.0
5	772.0
6	729.0
7	801.5
8	874.0
9	984.5
10	1095.0
11	1191.5
12	1288.0
13	1367.5
14	1447.0
15	1384.5
16	1322.0
17	1372.5
18	1423.0
19	1346.0
20	1269.0
21	1278.5
22	1288.0
23	1416.5
24	1545.0
25	1537.0
26	1909.5
27	2290.0
28	2564.0
29	2838.0
30	3316.5
31	3795.0
32	4830.5
33	5866.0
34	6424.5
35	6983.0
36	7783.0
37	8583.0
38	9686.5
39	10790.0
40	13124.0
41	15458.0
42	22892.0
43	30326.0
44	60355.0
45	90384.0
46	84927.0
47	79470.0
48	67304.5
49	55139.0
50	46355.0
51	37571.0
52	31815.5
53	26060.0
54	21426.0
55	16792.0
56	13577.0
57	10362.0
58	8433.0
59	6504.0
60	5469.0
61	4434.0
62	4019.0
63	3604.0
64	2882.0
65	2160.0
66	1828.0
67	1496.0
68	1070.0
69	644.0
70	543.0
71	442.0
72	355.0
73	268.0
74	234.0
75	152.5
76	105.0
77	69.0
78	33.0
79	43.5
80	54.0
81	42.0
82	30.0
83	19.5
84	9.0
85	7.0
86	5.0
87	4.0
88	3.0
89	1.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	442598.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.769920637247857
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.45363088356355	25.242355475119993
2	9.3253772165774	5.925329881661266
3	3.3611776260393627	3.2035303928089114
4	1.6155876186307785	2.0530836170568034
5	0.9776779601296803	1.553037560105316
6	0.6327820701484131	1.2062061689573098
7	0.4810008474176437	1.0696951124235254
8	0.34547361747493854	0.8780535527553378
9	0.30483053986653613	0.8715997853432347
>10	2.8835857394025965	20.291336643697914
>50	0.5148990354218842	10.716610652105198
>100	0.08759693133733298	5.437381762723363
>500	0.007833871908216772	1.6978242654575613
>1k	0.005697361387794015	4.56765408729441
>5k	7.121701734742519E-4	1.6602657862376846
>10k+	0.002136510520422756	13.626035256252155
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCC	22070	4.986466274135898	No Hit
AATCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCT	19420	4.387728819380115	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGT	18734	4.232734897130127	No Hit
CTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGCT	7338	1.6579379030180887	Illumina Single End Adapter 2 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTT	4778	1.079534927857785	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCT	3713	0.838910252644612	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2770	0.6258500942164221	No Hit
AACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTG	2490	0.5625872688082639	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTAT	2258	0.5101694991843615	No Hit
AAACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCT	1592	0.3596943501778137	No Hit
AATGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTC	1375	0.31066566048649114	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTT	1212	0.2738376585524562	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGG	836	0.18888472157578662	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATG	784	0.17713591114284294	No Hit
AATCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTTT	764	0.17261713789940306	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCT	701	0.15838300218256748	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	694	0.15680143154736353	No Hit
AATGATACGGCCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGC	655	0.14798982372265576	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCTTGGTTC	651	0.1470860690739678	No Hit
AACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTT	648	0.1464082530874518	No Hit
CCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGC	630	0.14234135716835594	No Hit
AATGATACCTGTCTCTTCTACACATCTGACGCTCCTTGGTTCGTATGCCGT	587	0.1326259946949602	No Hit
AATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTA	554	0.12517001884328444	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	492	0.11116182178862082	No Hit
TACCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCT	461	0.10415772326128903	No Hit
CTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTTCT	461	0.10415772326128903	No Hit
AATCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCC	454	0.10257615262608506	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2593866217199355E-4	0.0	0.0	2.5104044753930204	0.0
2	2.2593866217199355E-4	0.0	0.0	2.9939132124410865	0.0
3	2.2593866217199355E-4	0.0	0.0	4.449636012815241	0.0
4	2.2593866217199355E-4	0.0	0.0	13.04931337240566	0.0
5	2.2593866217199355E-4	0.0	0.0	13.892742398293711	0.0
6	2.2593866217199355E-4	0.0	0.0	17.270299459102844	0.0
7	2.2593866217199355E-4	0.0	0.0	19.897740161500955	0.0
8	2.2593866217199355E-4	0.0	0.0	20.889384949773834	0.0
9	2.2593866217199355E-4	0.0	0.0	27.809886172101997	0.0
10	2.2593866217199355E-4	0.0	0.0	29.969633843804083	0.0
11	2.2593866217199355E-4	0.0	0.0	38.2369554313395	0.0
12	2.2593866217199355E-4	0.0	0.0	39.66895467218559	0.0
13	2.2593866217199355E-4	0.0	0.0	40.4723925548692	0.0
14	2.2593866217199355E-4	0.0	0.0	41.604797129675234	0.0
15	2.2593866217199355E-4	0.0	0.0	42.15495777206404	0.0
16	2.2593866217199355E-4	0.0	0.0	42.964947875950635	0.0
17	2.2593866217199355E-4	0.0	0.0	43.889714820220604	0.0
18	2.2593866217199355E-4	0.0	0.0	45.38294343851531	0.0
19	2.2593866217199355E-4	0.0	0.0	45.906669257429996	0.0
20	2.2593866217199355E-4	0.0	0.0	46.43626948156115	0.0
21	2.2593866217199355E-4	0.0	0.0	46.96677346034099	0.0
22	2.2593866217199355E-4	0.0	0.0	47.539302030284816	0.0
23	2.2593866217199355E-4	0.0	0.0	48.088784856687106	0.0
24	2.2593866217199355E-4	0.0	0.0	48.465650545189995	0.0
25	2.2593866217199355E-4	0.0	0.0	48.77880153096037	0.0
26	2.2593866217199355E-4	0.0	0.0	49.08155933827084	0.0
27	2.2593866217199355E-4	0.0	0.0	49.43244208062395	0.0
28	2.2593866217199355E-4	0.0	0.0	49.73587770392094	0.0
29	2.2593866217199355E-4	0.0	0.0	50.05512903356997	0.0
30	2.2593866217199355E-4	0.0	0.0	50.572528569943834	0.0
31	2.2593866217199355E-4	0.0	0.0	50.896072734174126	0.0
32	2.2593866217199355E-4	0.0	0.0	51.17194384068613	0.0
33	2.2593866217199355E-4	0.0	0.0	51.44713713121162	0.0
34	2.2593866217199355E-4	0.0	0.0	51.724815747021	0.0
35	2.2593866217199355E-4	0.0	0.0	52.06056059900858	0.0
36	2.2593866217199355E-4	0.0	0.0	52.31632316458728	0.0
37	2.2593866217199355E-4	0.0	0.0	52.57954170601765	0.0
38	2.2593866217199355E-4	0.0	0.0	52.83711178089372	0.0
39	2.2593866217199355E-4	0.0	0.0	53.119535108608716	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCG	30	2.1624655E-6	45.000004	1
TTCTACG	30	2.1624655E-6	45.000004	1
GCGACGT	25	3.886904E-5	45.000004	28
CATATAG	25	3.886904E-5	45.000004	1
ATCTACG	30	2.1624655E-6	45.000004	1
ACGCATT	25	3.886904E-5	45.000004	14
ACGCATG	30	2.1624655E-6	45.000004	1
GGACTAT	50	2.1827873E-11	45.000004	8
GTCTCGC	65	0.0	45.000004	31
GGATCTA	50	2.1827873E-11	45.000004	8
GCAGTAC	65	0.0	45.000004	38
TGAACGG	25	3.886904E-5	45.000004	2
ATGCTAG	30	2.1624655E-6	45.000004	1
ATAGAGG	25	3.886904E-5	45.000004	2
ATCACCG	25	3.886904E-5	45.000004	1
ACGATAT	30	2.1624655E-6	45.000004	24
CCGTAGG	30	2.1624655E-6	45.000004	2
GAGACGG	30	2.1624655E-6	45.000004	2
TCTACGC	25	3.886904E-5	45.000004	11
TACGATA	30	2.1624655E-6	45.000004	23
>>END_MODULE