SRR3548817_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548817_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	440756
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCC	21100	4.787229215257422	No Hit
AATCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCT	18807	4.266986722812622	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGT	18452	4.186443292887675	No Hit
CTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGCT	7095	1.6097341839929575	Illumina Single End Adapter 2 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTT	4503	1.021653704090245	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCT	3673	0.8333408960967065	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2962	0.6720271533456152	No Hit
AACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTG	2515	0.570610496510541	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTAT	2196	0.4982348510286871	No Hit
AAACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCT	1537	0.34871901913984155	No Hit
AATGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTC	1382	0.31355216945430125	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTT	1079	0.24480665039159988	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCTTGGTTC	850	0.19285046601747907	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATG	822	0.18649774478396208	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGG	748	0.16970841009538157	No Hit
AATGATACGGCCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGC	695	0.15768361633193875	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	669	0.15178466090081588	No Hit
AACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTT	630	0.14293622775413153	No Hit
AATGATACAGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCC	613	0.13907921843378196	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCT	611	0.1386254526313879	No Hit
AATCTGTCACTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCT	593	0.13454156040984128	No Hit
AATGATCCGGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCC	583	0.13227273139787094	No Hit
AATGATACCTGTCTCTTCTACACATCTGACGCTCCTTGGTTCGTATGCCGT	580	0.13159208269427983	No Hit
AATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTA	561	0.12728130757153616	No Hit
AATGATACATGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGT	557	0.12637377596674804	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	552	0.12523936146076287	No Hit
CCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGC	545	0.12365118115238362	No Hit
AATCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTTT	530	0.12024793763442812	No Hit
CTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTTCT	465	0.10550054905662089	No Hit
AATCTGTCTCTTATACCCATCTGACGCTCCTTGGTTCGTATGCCGTCTTCT	445	0.1009628910326802	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	20	7.0285477E-4	45.000004	34
GACAGCG	20	7.0285477E-4	45.000004	1
GCCCAAC	20	7.0285477E-4	45.000004	11
CTCGTTG	20	7.0285477E-4	45.000004	35
GATAAGC	20	7.0285477E-4	45.000004	13
CGAGTCT	20	7.0285477E-4	45.000004	31
AAATACG	20	7.0285477E-4	45.000004	1
CACGGAG	20	7.0285477E-4	45.000004	4
ACGCATT	20	7.0285477E-4	45.000004	14
TTAGCGG	40	6.7993824E-9	45.000004	2
TAGACGG	40	6.7993824E-9	45.000004	2
CTATACG	35	1.2096461E-7	45.000004	1
GGCCGAT	40	6.7993824E-9	45.000004	8
ACGGGTA	40	6.7993824E-9	45.000004	5
TCGATAG	20	7.0285477E-4	45.000004	1
TTTCACG	40	6.7993824E-9	45.000004	1
TACATTA	20	7.0285477E-4	45.000004	13
GCACCCT	20	7.0285477E-4	45.000004	9
GCTCTCG	20	7.0285477E-4	45.000004	19
ACGATAT	20	7.0285477E-4	45.000004	24

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content