##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548817_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	440756
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.89123233716614	31.0	30.0	33.0	28.0	34.0
2	31.096432039495774	31.0	31.0	34.0	28.0	34.0
3	30.995820816959952	31.0	31.0	34.0	27.0	34.0
4	34.97243145867555	35.0	35.0	37.0	32.0	37.0
5	34.53201771501693	35.0	35.0	37.0	30.0	37.0
6	34.577521349681	35.0	35.0	37.0	31.0	37.0
7	34.334085072012634	35.0	35.0	37.0	30.0	37.0
8	34.80368276325223	35.0	35.0	37.0	32.0	37.0
9	35.50992839575638	38.0	35.0	39.0	32.0	39.0
10	35.33475891422919	37.0	35.0	39.0	30.0	39.0
11	35.798251186597575	37.0	35.0	39.0	30.0	39.0
12	35.2539999455481	37.0	34.0	39.0	30.0	39.0
13	35.19838413997768	37.0	34.0	39.0	30.0	39.0
14	35.98573587200174	38.0	34.0	40.0	30.0	41.0
15	36.23046084454891	38.0	35.0	40.0	31.0	41.0
16	36.03560473368485	38.0	34.0	40.0	30.0	41.0
17	36.00422455962029	38.0	34.0	40.0	30.0	41.0
18	35.645543112288884	37.0	34.0	40.0	29.0	41.0
19	35.63291027235024	37.0	34.0	40.0	29.0	41.0
20	35.824199784007476	37.0	34.0	40.0	30.0	41.0
21	36.080498053344705	37.0	34.0	40.0	30.0	41.0
22	36.05711323271833	37.0	34.0	40.0	30.0	41.0
23	36.016070115891786	38.0	34.0	40.0	30.0	41.0
24	35.571708609752335	37.0	34.0	40.0	29.0	41.0
25	35.671680022506784	37.0	34.0	40.0	29.0	41.0
26	35.59615524235632	37.0	34.0	40.0	29.0	41.0
27	35.61452821969525	37.0	34.0	40.0	29.0	41.0
28	35.47290110628103	37.0	34.0	40.0	29.0	41.0
29	35.31607737614463	37.0	34.0	40.0	28.0	41.0
30	35.43374338636343	37.0	34.0	40.0	29.0	41.0
31	35.11570347312345	37.0	34.0	40.0	27.0	41.0
32	35.125334652279264	37.0	34.0	40.0	27.0	41.0
33	34.78108522629301	37.0	34.0	40.0	26.0	41.0
34	34.96322228171596	37.0	34.0	40.0	27.0	41.0
35	34.433169372623404	37.0	33.0	40.0	25.0	41.0
36	34.54531532185609	37.0	33.0	40.0	25.0	41.0
37	34.40480674114476	37.0	33.0	40.0	25.0	40.0
38	33.9360507854686	36.0	33.0	39.0	24.0	40.0
39	33.864101679841	36.0	33.0	39.0	24.0	40.0
40	32.97624536024467	35.0	31.0	39.0	19.0	40.0
41	33.57824283730681	36.0	32.0	39.0	23.0	40.0
42	33.208564375754385	35.0	31.0	39.0	21.0	40.0
43	33.48348973127989	35.0	32.0	39.0	23.0	40.0
44	33.51736788608663	36.0	32.0	39.0	22.0	40.0
45	32.91508907422701	35.0	31.0	39.0	19.0	40.0
46	32.81505413426022	35.0	31.0	39.0	19.0	40.0
47	32.463351604969645	35.0	31.0	39.0	17.0	40.0
48	32.42453647823285	35.0	31.0	39.0	15.0	40.0
49	32.33679405385293	35.0	31.0	39.0	15.0	40.0
50	32.034111844194975	35.0	30.0	39.0	13.0	40.0
51	29.785563894762635	34.0	25.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	6.0
11	2.0
12	1.0
13	2.0
14	13.0
15	28.0
16	55.0
17	144.0
18	306.0
19	623.0
20	1172.0
21	1903.0
22	2659.0
23	3553.0
24	4538.0
25	5597.0
26	6974.0
27	8650.0
28	10786.0
29	13171.0
30	17050.0
31	21170.0
32	27202.0
33	34410.0
34	49194.0
35	52646.0
36	45432.0
37	51874.0
38	52982.0
39	28608.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.356242456143537	49.10721578378967	15.624744756736153	20.91179700333064
2	14.996052237519173	49.7168501393061	17.93350515931717	17.353592463857552
3	15.800352122262659	26.37559102995762	37.7528609933841	20.07119585439563
4	30.515750211001098	26.096525061485266	17.011679931753623	26.376044795760013
5	12.710887656662642	44.77420613672871	20.430124604089336	22.084781602519307
6	20.940611131782664	29.04078447031918	32.28135294811642	17.737251449781738
7	57.984916824728415	20.447367704580312	13.249280781203204	8.318434689488061
8	61.04148326965486	6.021472197769287	8.301872237700678	24.63517229487517
9	61.999155995607545	10.622884316946339	16.78547767926018	10.592482008185936
10	36.21119167975025	27.690831208196826	17.388759313543094	18.709217798509833
11	29.16715824628593	18.487326321139133	30.807521621940488	21.537993810634458
12	25.675430396863568	19.842044124186625	41.20874134441732	13.273784134532484
13	22.322101117171407	35.3354236811297	21.58291662507147	20.75955857662743
14	10.730426812113732	31.661281979144924	43.34938151721134	14.258909691530008
15	8.518318525442648	30.537530969516013	31.999564384829704	28.944586120211635
16	10.00757788889998	24.845038978482425	41.44469956166223	23.702683570955358
17	9.588298287487863	27.659294484930435	31.22180979952627	31.530597428055433
18	12.571354672426468	28.878336313062103	29.5977819927579	28.952527021753532
19	14.983119912150942	32.213288077757305	36.18351196580421	16.62008004428754
20	17.986822641098478	35.82685204512247	26.662597899971868	19.523727413807183
21	12.521213551261923	28.47493851473378	28.58543048761673	30.41841744638757
22	12.983147138099085	35.92554610714318	28.92666237101707	22.164644383740665
23	19.730871502600078	27.397017851146664	19.93120910435706	32.940901541896196
24	10.511257929557397	42.27872110646253	25.425859205546836	21.78416175843324
25	13.3645372950113	24.435515341821777	31.79468912504878	30.405258238118144
26	20.082993765257875	33.84026536224124	19.616522520396774	26.460218352104114
27	10.628329506575067	27.420386789969957	32.742832769151185	29.208450934303787
28	18.462596084908654	22.11904092060006	35.084264309504576	24.334098684986706
29	12.491491891205111	28.75241630289775	31.22317109693345	27.532920708963687
30	21.002323280908257	22.116998974489285	24.85297988002432	32.02769786457814
31	25.11956728893084	27.956737968399747	26.583642650355298	20.340052092314114
32	18.582163373839496	20.600740545789506	28.107615097695778	32.709480982675224
33	30.236003593825156	20.2263837588144	29.638394032072167	19.899218615288277
34	20.31123796386209	19.61289239397762	26.684832424289173	33.39103721787111
35	17.493352330994927	17.783081795823538	38.396981549882476	26.326584323299056
36	22.475247075479405	16.712648267975933	36.663823067638326	24.148281588906332
37	16.53772155115302	21.91235059760956	30.851083138970314	30.698844712267103
38	27.629345941972428	20.344589750338056	35.406891795006764	16.619172512682752
39	23.452431730935032	19.894907840165533	36.6660918966503	19.986568532249134
40	26.467024839140024	24.25423590376535	27.533828240568475	21.74491101652615
41	23.58243563332093	16.51775585584768	38.014003212661876	21.885805298169508
42	25.224840955086258	19.766265235186815	29.51179337320422	25.4971004365227
43	21.22308034377297	19.08334770258374	31.58709127045349	28.106480683189794
44	18.812903284356878	18.270199384693573	28.357866937716107	34.55903039323344
45	28.46087177485956	26.943252048752598	24.452985325213948	20.14289085117389
46	15.945330296127564	19.517374692573668	44.44930982221456	20.087985189084208
47	24.098367350642985	23.757816115946238	23.42702084600096	28.716795687409814
48	18.697419887647587	15.840056629972137	42.05705651199303	23.40546697038724
49	22.627258619281417	14.711994845220486	35.113759086660195	27.546987448837907
50	19.58067502200764	18.168328962056105	25.876221764422947	36.37477425151331
51	15.045285827078928	18.211209830382344	39.97563277641144	26.767871566127287
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3432.0
1	2723.0
2	2014.0
3	1433.5
4	853.0
5	786.5
6	720.0
7	795.0
8	870.0
9	971.5
10	1073.0
11	1137.0
12	1201.0
13	1310.0
14	1419.0
15	1420.5
16	1422.0
17	1415.0
18	1408.0
19	1369.0
20	1330.0
21	1342.5
22	1355.0
23	1504.0
24	1653.0
25	1654.5
26	2031.0
27	2406.0
28	2718.0
29	3030.0
30	3568.0
31	4106.0
32	5085.5
33	6065.0
34	6683.0
35	7301.0
36	8167.5
37	9034.0
38	10059.0
39	11084.0
40	13456.0
41	15828.0
42	23036.0
43	30244.0
44	59758.0
45	89272.0
46	83352.0
47	77432.0
48	65622.0
49	53812.0
50	45305.0
51	36798.0
52	31480.5
53	26163.0
54	21555.0
55	16947.0
56	13673.5
57	10400.0
58	8565.0
59	6730.0
60	5681.0
61	4632.0
62	4113.0
63	3594.0
64	2894.0
65	2194.0
66	1823.5
67	1453.0
68	1039.0
69	625.0
70	523.5
71	422.0
72	354.0
73	286.0
74	240.5
75	156.0
76	117.0
77	84.0
78	51.0
79	55.0
80	59.0
81	53.0
82	47.0
83	31.0
84	15.0
85	9.0
86	3.0
87	1.5
88	0.0
89	2.0
90	4.0
91	2.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	440756.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.776879690060316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.35763191558335	25.217379218695836
2	9.50882827076549	6.043217839071184
3	3.2996014735714985	3.145531171524817
4	1.639274311328937	2.083640902804244
5	0.9425717401184579	1.497599439249752
6	0.6241055458190553	1.1899276106034928
7	0.4582665401549267	1.0193596498738309
8	0.36189149048702807	0.919982588325032
9	0.3159000527004	0.9034486171869893
>10	2.8486799881869036	19.987393869507205
>50	0.5405873320053504	11.424515360763783
>100	0.08255981830094992	4.7952896999391905
>500	0.011486583415784337	2.313008671155255
>1k	0.005743291707892169	4.527693371774173
>5k	7.179114634865211E-4	1.6185813711260018
>10k+	0.002153734390459563	13.313430618399202
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCC	21100	4.787229215257422	No Hit
AATCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCT	18807	4.266986722812622	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGT	18452	4.186443292887675	No Hit
CTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGCT	7095	1.6097341839929575	Illumina Single End Adapter 2 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTT	4503	1.021653704090245	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCT	3673	0.8333408960967065	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2962	0.6720271533456152	No Hit
AACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTG	2515	0.570610496510541	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTAT	2196	0.4982348510286871	No Hit
AAACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCT	1537	0.34871901913984155	No Hit
AATGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTC	1382	0.31355216945430125	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTT	1079	0.24480665039159988	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCTTGGTTC	850	0.19285046601747907	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATG	822	0.18649774478396208	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGG	748	0.16970841009538157	No Hit
AATGATACGGCCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGC	695	0.15768361633193875	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	669	0.15178466090081588	No Hit
AACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTT	630	0.14293622775413153	No Hit
AATGATACAGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCC	613	0.13907921843378196	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCT	611	0.1386254526313879	No Hit
AATCTGTCACTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCT	593	0.13454156040984128	No Hit
AATGATCCGGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCC	583	0.13227273139787094	No Hit
AATGATACCTGTCTCTTCTACACATCTGACGCTCCTTGGTTCGTATGCCGT	580	0.13159208269427983	No Hit
AATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTA	561	0.12728130757153616	No Hit
AATGATACATGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGT	557	0.12637377596674804	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	552	0.12523936146076287	No Hit
CCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGC	545	0.12365118115238362	No Hit
AATCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTTT	530	0.12024793763442812	No Hit
CTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTTCT	465	0.10550054905662089	No Hit
AATCTGTCTCTTATACCCATCTGACGCTCCTTGGTTCGTATGCCGTCTTCT	445	0.1009628910326802	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.5376580239406836E-4	0.0	0.0	2.335986350724664	0.0
2	4.5376580239406836E-4	0.0	0.0	2.7784080080588804	0.0
3	4.5376580239406836E-4	0.0	0.0	4.126546206971658	0.0
4	4.5376580239406836E-4	0.0	0.0	12.005508716841064	0.0
5	4.5376580239406836E-4	0.0	0.0	12.825690404668343	0.0
6	4.5376580239406836E-4	0.0	0.0	15.96234651371734	0.0
7	4.5376580239406836E-4	0.0	0.0	18.43491637096262	0.0
8	4.5376580239406836E-4	0.0	0.0	19.371715870005172	0.0
9	4.5376580239406836E-4	0.0	0.0	26.023015001497427	0.0
10	4.5376580239406836E-4	0.0	0.0	28.08764940239044	0.0
11	4.5376580239406836E-4	0.0	0.0	36.03444989971776	0.0
12	4.5376580239406836E-4	0.0	0.0	37.432956102696274	0.0
13	4.5376580239406836E-4	0.0	0.0	38.23203768071223	0.0
14	4.5376580239406836E-4	0.0	0.0	39.31971430905081	0.0
15	4.5376580239406836E-4	0.0	0.0	39.8488052346423	0.0
16	4.5376580239406836E-4	0.0	0.0	40.63903837951157	0.0
17	4.5376580239406836E-4	0.0	0.0	41.52297416257521	0.0
18	4.5376580239406836E-4	0.0	0.0	43.07031554873898	0.0
19	4.5376580239406836E-4	0.0	0.0	43.61460762871067	0.0
20	4.5376580239406836E-4	0.0	0.0	44.153454519053625	0.0
21	4.5376580239406836E-4	0.0	0.0	44.64964742397154	0.0
22	4.5376580239406836E-4	0.0	0.0	45.22479557850602	0.0
23	4.5376580239406836E-4	0.0	0.0	45.74821443156758	0.0
24	4.5376580239406836E-4	0.0	0.0	46.12506693045585	0.0
25	4.5376580239406836E-4	0.0	0.0	46.42024158491319	0.0
26	6.806487035911025E-4	0.0	0.0	46.71745818548131	0.0
27	6.806487035911025E-4	0.0	0.0	47.06708473622594	0.0
28	6.806487035911025E-4	0.0	0.0	47.380863788581436	0.0
29	6.806487035911025E-4	0.0	0.0	47.6898783000118	0.0
30	6.806487035911025E-4	0.0	0.0	48.1792647178938	0.0
31	6.806487035911025E-4	0.0	0.0	48.506883627222315	0.0
32	6.806487035911025E-4	0.0	0.0	48.767345197796516	0.0
33	6.806487035911025E-4	0.0	0.0	49.03552078701141	0.0
34	6.806487035911025E-4	0.0	0.0	49.30052001560954	0.0
35	6.806487035911025E-4	0.0	0.0	49.656045521785295	0.0
36	6.806487035911025E-4	0.0	0.0	49.91287696594034	0.0
37	6.806487035911025E-4	0.0	0.0	50.16380945466426	0.0
38	6.806487035911025E-4	0.0	0.0	50.42245596202888	0.0
39	6.806487035911025E-4	0.0	0.0	50.68541324451624	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	20	7.0285477E-4	45.000004	34
GACAGCG	20	7.0285477E-4	45.000004	1
GCCCAAC	20	7.0285477E-4	45.000004	11
CTCGTTG	20	7.0285477E-4	45.000004	35
GATAAGC	20	7.0285477E-4	45.000004	13
CGAGTCT	20	7.0285477E-4	45.000004	31
AAATACG	20	7.0285477E-4	45.000004	1
CACGGAG	20	7.0285477E-4	45.000004	4
ACGCATT	20	7.0285477E-4	45.000004	14
TTAGCGG	40	6.7993824E-9	45.000004	2
TAGACGG	40	6.7993824E-9	45.000004	2
CTATACG	35	1.2096461E-7	45.000004	1
GGCCGAT	40	6.7993824E-9	45.000004	8
ACGGGTA	40	6.7993824E-9	45.000004	5
TCGATAG	20	7.0285477E-4	45.000004	1
TTTCACG	40	6.7993824E-9	45.000004	1
TACATTA	20	7.0285477E-4	45.000004	13
GCACCCT	20	7.0285477E-4	45.000004	9
GCTCTCG	20	7.0285477E-4	45.000004	19
ACGATAT	20	7.0285477E-4	45.000004	24
>>END_MODULE