##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548814_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	540321
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28835451518634	31.0	31.0	34.0	30.0	34.0
2	31.46894161063516	31.0	31.0	34.0	30.0	34.0
3	31.638394583960274	31.0	31.0	34.0	30.0	34.0
4	35.46948758238158	37.0	35.0	37.0	33.0	37.0
5	35.02654903288971	37.0	35.0	37.0	32.0	37.0
6	35.04533971472514	36.0	35.0	37.0	32.0	37.0
7	34.9584543262246	36.0	35.0	37.0	32.0	37.0
8	35.332343181183035	37.0	35.0	37.0	33.0	37.0
9	36.20257032393707	39.0	37.0	39.0	32.0	39.0
10	35.932721474827	38.0	35.0	39.0	30.0	39.0
11	36.19140103753139	38.0	35.0	39.0	32.0	39.0
12	35.69660812739094	37.0	35.0	39.0	31.0	39.0
13	35.51480138658316	37.0	35.0	39.0	30.0	39.0
14	36.470459227015056	38.0	35.0	40.0	31.0	41.0
15	36.59619744559253	38.0	35.0	40.0	31.0	41.0
16	36.56830291622943	38.0	35.0	40.0	31.0	41.0
17	36.48780262103453	38.0	35.0	40.0	31.0	41.0
18	36.24091234654955	38.0	35.0	40.0	30.0	41.0
19	36.26315097877003	38.0	34.0	40.0	30.0	41.0
20	36.287105257800455	38.0	34.0	40.0	30.0	41.0
21	36.390553023110336	38.0	35.0	40.0	31.0	41.0
22	36.342242851934316	38.0	35.0	40.0	31.0	41.0
23	36.269256608571574	38.0	35.0	40.0	31.0	41.0
24	35.870090187129506	38.0	34.0	40.0	30.0	41.0
25	35.95052200451213	38.0	34.0	40.0	30.0	41.0
26	35.86398640807964	38.0	34.0	40.0	30.0	41.0
27	35.73706555917686	38.0	34.0	40.0	29.0	41.0
28	35.77121562922781	38.0	34.0	40.0	29.0	41.0
29	35.46777933857836	37.0	34.0	40.0	29.0	41.0
30	35.38168051954301	37.0	34.0	40.0	28.0	41.0
31	35.26080237488456	37.0	34.0	40.0	27.0	41.0
32	34.94109612619165	37.0	34.0	40.0	26.0	41.0
33	35.01541861226937	37.0	34.0	40.0	27.0	41.0
34	34.8970167733625	37.0	34.0	40.0	26.0	41.0
35	34.76678678045088	37.0	33.0	40.0	25.0	41.0
36	34.70268969742061	37.0	33.0	40.0	25.0	41.0
37	34.324967935727095	37.0	33.0	40.0	24.0	41.0
38	34.35339733232652	37.0	33.0	40.0	24.0	41.0
39	34.12031551614688	36.0	33.0	40.0	23.0	41.0
40	34.01654757079588	36.0	33.0	40.0	23.0	41.0
41	34.340356935969545	37.0	33.0	40.0	24.0	41.0
42	34.30398226239587	36.0	33.0	40.0	24.0	41.0
43	34.277448035519626	36.0	33.0	40.0	24.0	41.0
44	34.06478186115291	36.0	33.0	40.0	23.0	41.0
45	33.78482605710309	36.0	33.0	39.0	23.0	41.0
46	33.632211222588055	35.0	32.0	39.0	23.0	40.0
47	33.597554046576015	35.0	32.0	39.0	23.0	40.0
48	33.61990927615251	35.0	32.0	39.0	23.0	40.0
49	33.514777326811284	35.0	32.0	39.0	22.0	40.0
50	33.26578274766296	35.0	32.0	39.0	22.0	40.0
51	31.135826666000398	34.0	28.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	2.0
10	7.0
11	10.0
12	10.0
13	24.0
14	32.0
15	49.0
16	112.0
17	253.0
18	539.0
19	935.0
20	1625.0
21	2427.0
22	3263.0
23	4226.0
24	5256.0
25	5903.0
26	6861.0
27	8242.0
28	9919.0
29	13065.0
30	16467.0
31	21234.0
32	27798.0
33	36737.0
34	57203.0
35	62325.0
36	55887.0
37	68357.0
38	78520.0
39	53029.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.340023800666643	38.11326970449048	18.499003370218816	25.047703124624064
2	21.313441454246643	38.12566974076521	19.357567075867863	21.203321729120283
3	19.040348237436636	30.056947629279634	26.43780271357212	24.464901419711616
4	24.40919379405946	31.404294854355097	18.08850664697467	26.098004704610776
5	15.727872875568414	40.12577708436281	18.486418258775803	25.659931781292972
6	20.247038334619603	34.66772529662923	23.92614760484971	21.159088763901458
7	72.83059514621864	13.007267901858341	8.27026896974206	5.891867982180963
8	75.1031331375238	6.435433751418139	5.941283052111615	12.520150058946442
9	70.44849265529194	11.03825318653171	11.54332332076673	6.969930837409614
10	41.50625276456032	28.237288574754636	13.766631317309525	16.489827343375513
11	33.21136879743708	23.024646460159794	24.203945432437386	19.56003930996574
12	29.86243362741778	23.68147823238408	30.18557487123395	16.27051326896419
13	22.86159523690547	34.940711169841634	22.220494853984945	19.977198739267955
14	15.797831289178099	34.32367055879745	32.95985164374511	16.918646508279338
15	14.27336712805906	28.834155992456335	32.49383237001708	24.39864450946752
16	16.060638028135127	24.920556484015982	35.81241521243853	23.206390275410357
17	15.36086881686997	26.70412588072646	28.504537117750374	29.430468184653197
18	19.609269304728116	27.444241478676563	27.85955015629598	25.08693906029934
19	21.045637685746065	30.987875725726006	28.3564769831267	19.61000960540123
20	24.33386820056966	31.023040007699127	24.276124748066426	20.366967043664786
21	18.715541317105945	30.13208814760115	26.31657847834898	24.83579205694393
22	18.568776708660224	30.3475156434786	25.301626255503674	25.782081392357508
23	22.454614941858637	29.35033063678813	20.114339438963135	28.0807149823901
24	17.232719068849814	34.1191624978485	26.45131320085653	22.196805232445158
25	17.993378010478956	26.34045317505705	27.063726932693715	28.60244188177028
26	22.225306808360216	31.77925714528956	22.45368956601724	23.541746480332986
27	16.059527577125447	29.892045654342514	27.361142728119027	26.68728404041301
28	21.07025268312725	27.19364970082599	26.837380001887766	24.89871761415899
29	21.854786321464463	30.05250582524092	26.541444807808688	21.551263045485925
30	22.59471684424629	30.53481171377755	22.61377958657909	24.25669185539707
31	30.516859422454427	28.35740235896809	17.575848430840185	23.54988978773729
32	24.991810423803628	29.462486188765567	20.357158059745966	25.188545327684842
33	26.072279256219915	29.513937085547294	20.22612484060401	24.18765881762878
34	21.807407078384884	26.553104543410306	21.83794448115102	29.80154389705379
35	19.591317013404993	29.473405623694067	26.31676355351726	24.618513809383682
36	29.472110097516108	26.176661651129606	24.34700853751751	20.004219713836775
37	20.651427577310525	34.08048178767806	21.022133139374557	24.24595749563685
38	25.70601549819459	31.583077466913185	22.931738725683438	19.779168309208785
39	20.659570884714825	28.91947564503323	24.845601040862746	25.5753524293892
40	21.709687389533258	29.5764924924258	23.68332898406688	25.030491133974063
41	19.118635033618904	21.321214611314385	30.24461384991514	29.315536505151567
42	24.67718263772831	23.540080803818473	25.334199485120884	26.448537073332336
43	23.418856568595334	23.215458958656058	27.240843868737286	26.124840604011318
44	21.19092169284555	23.871735505375508	25.53685679438704	29.4004860073919
45	26.682657161206023	28.21248850220517	23.684069284739998	21.42078505184881
46	19.321292342885062	25.29533369978217	32.8447348890752	22.538639068257574
47	22.725564988219965	26.346930805946833	23.80270246760722	27.12480173822598
48	21.68100073844992	20.50614357021104	33.88208120728234	23.9307744840567
49	23.83897720059002	19.693293431127053	30.55683565880282	25.910893709480103
50	20.73989350774817	22.83809069053396	25.248879832543984	31.17313596917388
51	18.431080783460203	23.044449503165712	31.64637317446481	26.87809653890928
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3593.0
1	2866.0
2	2139.0
3	1588.5
4	1038.0
5	1030.5
6	1023.0
7	1072.0
8	1121.0
9	1264.5
10	1408.0
11	1558.5
12	1709.0
13	1753.5
14	1798.0
15	1829.0
16	1860.0
17	1791.0
18	1722.0
19	1736.5
20	1751.0
21	1605.5
22	1460.0
23	1628.5
24	1797.0
25	1889.5
26	2667.5
27	3353.0
28	3826.0
29	4299.0
30	4248.0
31	4197.0
32	5269.5
33	6342.0
34	7543.5
35	8745.0
36	9241.5
37	9738.0
38	11827.0
39	13916.0
40	16391.0
41	18866.0
42	25146.0
43	31426.0
44	50306.5
45	69187.0
46	67456.5
47	65726.0
48	60676.5
49	55627.0
50	52193.0
51	48759.0
52	43362.5
53	37966.0
54	33960.0
55	29954.0
56	26846.0
57	23738.0
58	21221.5
59	18705.0
60	17513.0
61	16321.0
62	15056.5
63	13792.0
64	12248.0
65	10704.0
66	9165.0
67	7626.0
68	6449.5
69	5273.0
70	4537.5
71	3802.0
72	3232.0
73	2662.0
74	2428.0
75	1904.5
76	1615.0
77	1089.5
78	564.0
79	518.5
80	473.0
81	320.5
82	168.0
83	130.5
84	93.0
85	69.0
86	45.0
87	35.0
88	25.0
89	16.0
90	7.0
91	8.5
92	10.0
93	6.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	540321.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.11466707643122
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.43073905988425	23.125995233634114
2	9.120500762903722	5.310806865645577
3	3.2599498594694167	2.847370645329325
4	1.539549618996852	1.7929389841895953
5	0.8945577365571669	1.302237534025389
6	0.5826142315809548	1.017757163188218
7	0.39838881195372766	0.8119270338905421
8	0.2922636170722355	0.6807326327689376
9	0.25650029188838497	0.6721128543003986
>10	2.8549342416691514	20.13853314354474
>50	1.13077496554838	23.19551123442479
>100	0.22843938697947072	9.316299581196148
>500	0.0031727692636037605	0.5479632847051733
>1k	0.005076430821766016	2.461585571615553
>5k	0.001903661558162256	4.66175238131006
>10k+	6.34553852720752E-4	2.116475856231445
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCC	11456	2.1202211278110608	No Hit
AATCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCT	9958	1.8429785257282245	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGT	9870	1.82669191091962	No Hit
CTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGCT	5405	1.0003312845512204	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2924	0.5411597920495409	No Hit
AATGACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTT	2221	0.4110519487489844	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCT	1835	0.33961293379306007	No Hit
AACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTG	1573	0.2911232397038057	No Hit
AAACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCT	1440	0.2665082423226193	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTT	1158	0.21431704486777306	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTAT	1105	0.20450806094895443	No Hit
GGCGACGGGCAGATTGCGATTTAAAACAAACTGGTTGTTGGGCTTATCATC	1068	0.19766027972260933	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	745	0.1378810003682996	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCT	596	0.11030480029463967	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	561	0.10382716940485377	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	545	0.10086596671238023	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.3334665874544946	0.0
2	0.0	0.0	0.0	1.6110793398738898	0.0
3	0.0	0.0	0.0	2.28105144904603	0.0
4	0.0	0.0	0.0	6.064913264522386	0.0
5	0.0	0.0	0.0	6.454311418582657	0.0
6	0.0	0.0	0.0	8.478478534056608	0.0
7	0.0	0.0	0.0	10.093629527632649	0.0
8	0.0	0.0	0.0	11.114504155862903	0.0
9	0.0	0.0	0.0	14.388298807560691	0.0
10	0.0	0.0	0.0	16.225354927903968	0.0
11	0.0	0.0	0.0	20.362525239626073	0.0
12	0.0	0.0	0.0	21.355268442277833	0.0
13	0.0	0.0	0.0	21.857747524156938	0.0
14	0.0	0.0	0.0	22.467940353974768	0.0
15	0.0	0.0	0.0	22.802371183056	0.0
16	0.0	0.0	0.0	23.37628927989103	0.0
17	0.0	0.0	0.0	24.006655303051335	0.0
18	0.0	0.0	0.0	24.96349392305685	0.0
19	0.0	0.0	0.0	25.323465125360666	0.0
20	0.0	0.0	0.0	25.689913958554268	0.0
21	0.0	0.0	0.0	26.04655380782905	0.0
22	0.0	0.0	0.0	26.447796772659217	0.0
23	0.0	0.0	0.0	26.85921887174476	0.0
24	0.0	0.0	0.0	27.117583806663077	0.0
25	0.0	0.0	0.0	27.364844231484618	0.0
26	0.0	0.0	0.0	27.573794096472284	0.0
27	0.0	0.0	0.0	27.820314220620705	0.0
28	0.0	0.0	0.0	28.059801488374504	0.0
29	0.0	0.0	0.0	28.284112592329375	0.0
30	0.0	0.0	0.0	28.668513716846096	0.0
31	0.0	0.0	0.0	28.930024929625166	0.0
32	0.0	0.0	0.0	29.17598982826875	0.0
33	0.0	0.0	0.0	29.44712494979836	0.0
34	0.0	0.0	0.0	29.684391315532803	0.0
35	1.8507516827959676E-4	0.0	0.0	29.96718617266403	0.0
36	1.8507516827959676E-4	0.0	0.0	30.19075697594578	0.0
37	1.8507516827959676E-4	0.0	0.0	30.417288981920006	0.0
38	1.8507516827959676E-4	0.0	0.0	30.676579292679722	0.0
39	1.8507516827959676E-4	0.0	0.0	30.904776975168463	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCG	45	3.8380676E-10	45.000004	1
GTAATCC	45	3.8380676E-10	45.000004	9
TAGCACG	45	3.8380676E-10	45.000004	1
ATTGCGA	145	0.0	45.000004	13
AACCGTA	40	6.8030204E-9	45.0	29
TCGCAAG	25	3.8880517E-5	45.0	1
TGCCCGT	20	7.029965E-4	45.0	9
TGCCCGA	20	7.029965E-4	45.0	33
ACACGGT	25	3.8880517E-5	45.0	3
GTCGATA	20	7.029965E-4	45.0	12
GGTGTAT	25	3.8880517E-5	45.0	9
AATCAAT	25	3.8880517E-5	45.0	13
ACGCATA	25	3.8880517E-5	45.0	32
ACGTTCG	25	3.8880517E-5	45.0	41
ACGTTAG	20	7.029965E-4	45.0	1
TAGACCG	20	7.029965E-4	45.0	1
ACGCAAT	20	7.029965E-4	45.0	27
ACCGCAC	20	7.029965E-4	45.0	22
CTATAGT	20	7.029965E-4	45.0	20
GCTATTA	20	7.029965E-4	45.0	18
>>END_MODULE