FastQCFastQC Report
Sat 18 Jun 2016
SRR3548813_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548813_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548782
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCC106821.9464924141097923No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGT91801.6727953905193684No Hit
AATCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCT89271.6266932953340307No Hit
CTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTGCT51750.9429974015182714No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30510.5559584680255547No Hit
AATGACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTT20910.38102561672941165No Hit
AATGATCTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCT17300.3152435757732579No Hit
AACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCTG15110.27533701907132524No Hit
AAACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTTCT12830.23379046688849123No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTAT11630.21192386047647332No Hit
GGCGACGGGCAGATTGCGATTTAAAACAAACTGGTTGTTGGGCTTATCATC10470.19078614094485608No Hit
AAAAACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCTT9980.18185727665994875No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7550.13757739867561256No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5880.10714637141888764No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCGAGAACTTCGTATGCCGTCT5490.10003972433498183No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAA207.030059E-445.00000413
CATGGAT207.030059E-445.00000444
CGTGGAC207.030059E-445.00000422
TCGTTAC207.030059E-445.00000439
TCGCAAG302.1634205E-645.0000041
ACACGAG302.1634205E-645.0000041
GTCGATA302.1634205E-645.00000412
CTCGTCG207.030059E-445.00000413
CCCTAAG207.030059E-445.00000433
TCTTCGA207.030059E-445.00000412
ATGGATG302.1634205E-645.00000418
TTTCGGT207.030059E-445.00000429
ATAATGC207.030059E-445.00000418
CGCATTA302.1634205E-645.00000434
CGTTGCC207.030059E-445.00000425
CACGCTT207.030059E-445.00000414
TAAACGG750.045.0000042
CGCGCGT207.030059E-445.00000417
TGAACGG406.8030204E-945.0000042
ATTCGTT207.030059E-445.00000437