##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548811_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	368085
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.126291481587135	31.0	31.0	34.0	28.0	34.0
2	31.328676800195606	31.0	31.0	34.0	28.0	34.0
3	31.31854055449149	31.0	31.0	34.0	28.0	34.0
4	35.336607033701455	37.0	35.0	37.0	33.0	37.0
5	34.99560155942242	35.0	35.0	37.0	32.0	37.0
6	35.04153388483638	36.0	35.0	37.0	32.0	37.0
7	34.92984229186193	36.0	35.0	37.0	32.0	37.0
8	35.33200483583955	37.0	35.0	37.0	33.0	37.0
9	35.774614559137156	39.0	35.0	39.0	32.0	39.0
10	35.69231835038103	38.0	35.0	39.0	30.0	39.0
11	36.07406713123328	38.0	35.0	39.0	31.0	39.0
12	35.5659643832267	37.0	35.0	39.0	31.0	39.0
13	35.145056169091376	37.0	34.0	39.0	30.0	39.0
14	36.08957441895215	38.0	35.0	40.0	30.0	41.0
15	36.28570303055001	38.0	35.0	40.0	31.0	41.0
16	36.35983264735047	38.0	35.0	40.0	31.0	41.0
17	36.33413477865167	38.0	35.0	40.0	31.0	41.0
18	36.16623062607822	38.0	35.0	40.0	30.0	41.0
19	36.14517570669818	38.0	34.0	40.0	30.0	41.0
20	36.08133990790171	38.0	34.0	40.0	30.0	41.0
21	36.0874825108331	38.0	34.0	40.0	30.0	41.0
22	36.06412105899452	38.0	34.0	40.0	30.0	41.0
23	36.02708613499599	38.0	34.0	40.0	30.0	41.0
24	35.683907249684175	37.0	34.0	40.0	29.0	41.0
25	35.78136300039393	37.0	34.0	40.0	30.0	41.0
26	35.69090834997351	37.0	34.0	40.0	30.0	41.0
27	35.584354157327795	37.0	34.0	40.0	29.0	41.0
28	35.44332694893843	37.0	34.0	40.0	29.0	41.0
29	35.29037314750669	37.0	34.0	40.0	28.0	41.0
30	35.280318404716304	37.0	34.0	40.0	28.0	41.0
31	35.09933575125311	37.0	34.0	40.0	27.0	41.0
32	35.0890147656112	37.0	34.0	40.0	27.0	41.0
33	34.811445725851364	37.0	34.0	40.0	27.0	41.0
34	34.919839167583575	37.0	34.0	40.0	27.0	41.0
35	34.77624733417553	37.0	34.0	40.0	26.0	41.0
36	34.47966638140647	36.0	33.0	40.0	25.0	41.0
37	34.43195729247321	36.0	33.0	40.0	25.0	41.0
38	34.271002621677056	36.0	33.0	40.0	25.0	41.0
39	34.207270059904644	36.0	33.0	40.0	24.0	41.0
40	33.95327437955907	36.0	33.0	39.0	23.0	41.0
41	34.0350299523208	36.0	33.0	39.0	24.0	41.0
42	34.08232880992162	36.0	33.0	39.0	24.0	41.0
43	33.955868888979445	35.0	33.0	39.0	24.0	41.0
44	33.93529755355421	35.0	33.0	39.0	24.0	41.0
45	33.734542293220315	35.0	33.0	39.0	23.0	40.0
46	33.570504095521414	35.0	33.0	39.0	23.0	40.0
47	33.42778162652648	35.0	32.0	39.0	23.0	40.0
48	33.41003844220764	35.0	33.0	39.0	23.0	40.0
49	33.271518806797346	35.0	32.0	39.0	22.0	40.0
50	33.05722591249304	35.0	32.0	39.0	22.0	40.0
51	31.26158631837755	34.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	8.0
10	13.0
11	13.0
12	9.0
13	17.0
14	29.0
15	41.0
16	86.0
17	216.0
18	416.0
19	766.0
20	1296.0
21	1762.0
22	2350.0
23	3073.0
24	3644.0
25	4036.0
26	4738.0
27	5581.0
28	7102.0
29	8854.0
30	11282.0
31	14453.0
32	19401.0
33	26726.0
34	43488.0
35	44711.0
36	36291.0
37	45312.0
38	50722.0
39	31645.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.495225287637368	35.6129698303381	19.682410312835348	25.20939456918918
2	22.442914000842194	35.37117785294158	20.031786136354373	22.15412200986185
3	20.108398875259788	32.415882201122024	22.132387899534077	25.343331024084108
4	20.749555129929227	33.288235054403195	18.817392721789805	27.14481709387777
5	16.37610877922219	37.94775663229961	18.415855033484114	27.260279554994092
6	20.915277721178533	37.24085469388864	20.629474170368255	21.21439341456457
7	75.5347270331581	11.48321719168127	7.267071464471521	5.714984310689107
8	78.64976839588682	7.261094584131382	5.920915006044799	8.168222013937
9	72.59709034597986	12.434356194900634	9.597783120746566	5.37077033837293
10	45.04883382914272	26.94459160248312	12.077373432766889	15.929201135607265
11	37.56496461415163	24.50222095439912	21.47139926919054	16.461415162258717
12	34.423298966271375	24.4160995422253	26.776152247442848	14.384449244060477
13	24.486192048032386	36.4144151486749	20.29096540201312	18.808427401279594
14	16.154149177499765	37.928739285762795	29.52171373459935	16.395397802138092
15	14.677588057106375	28.280696034883245	36.393496067484406	20.648219840525968
16	15.620033416194628	25.490851297933904	36.65294700952226	22.236168276349215
17	15.135906108643384	26.058383253867994	27.503973267044294	31.30173737044433
18	21.50671719847318	26.699539508537427	26.476493201298613	25.31725009169078
19	23.83199532716628	31.242783596180228	25.628319545757094	19.296901530896395
20	27.379545485417772	29.16744773625657	22.546151024899142	20.906855753426516
21	19.61992474564299	31.958107502343207	24.06563701318989	24.356330738823914
22	19.22898243612209	30.047679204531562	24.613064917070783	26.11027344227556
23	21.05220261624353	30.27235557004496	20.941086977192768	27.734354836518737
24	17.88065256666259	29.619788907453444	28.340736514663732	24.158822011220234
25	18.247415678443836	26.632163766521323	28.733852235217412	26.38656831981743
26	21.02775174212478	32.500916907779455	22.22964804325088	24.241683306844884
27	17.768178545716342	30.902644769550513	27.035059836722496	24.29411684801065
28	18.09962372821495	29.17233791108032	32.281674069848	20.44636429085673
29	18.292242280994877	29.08023961856636	27.89382887104881	24.733689229389952
30	20.692231413939716	28.86697366097505	27.691429968621378	22.74936495646386
31	27.85253406142603	30.72279500658815	19.05130608419251	22.373364847793308
32	26.314574079356674	25.844302267139383	21.92944564434845	25.91167800915549
33	24.14469483950718	30.367442302728993	23.796949074262738	21.69091378350109
34	20.636537756224786	27.513753616691794	24.626377059646547	27.22333156743687
35	22.23834168738199	26.995938438132494	27.78760340682179	22.97811646766372
36	25.815232894575978	27.04918700843555	26.570221552087155	20.565358544901315
37	22.21986769360338	30.792615836016136	23.53505304481302	23.452463425567462
38	22.671393835662958	32.04776070744529	22.9311164540799	22.34972900281185
39	23.582868087534127	27.920996508958527	23.44485648695274	25.051278916554597
40	26.9858864121059	27.688984881209507	21.677601640925328	23.647527065759267
41	22.315226102666504	21.935150848309494	28.113071708980264	27.63655134004374
42	27.641984867625684	23.198989363869757	23.41361370335656	25.745412065147992
43	24.252550362008776	23.406278441120936	25.749487210834452	26.591683986035836
44	21.5730062349729	25.538122987896816	25.967099990491327	26.921770786638955
45	24.060475161987043	30.231875789559474	23.068313025523995	22.639336022929488
46	21.2896477715745	25.3441460532214	29.90206066533545	23.464145509868644
47	22.930573101321706	27.04348180447451	24.04987978320225	25.976065311001534
48	24.601111156390505	20.79356670334298	31.868725973620222	22.73659616664629
49	24.92820951682356	20.306179279242567	28.094054362443455	26.671556841490418
50	19.64736405993181	25.488406210522026	25.978238722034313	28.88599100751185
51	19.632150182702365	26.021978619068964	28.784112365350396	25.561758832878272
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3379.0
1	2748.0
2	2117.0
3	1478.0
4	839.0
5	795.0
6	751.0
7	796.0
8	841.0
9	873.0
10	905.0
11	949.5
12	994.0
13	1060.5
14	1127.0
15	1182.5
16	1238.0
17	1212.0
18	1186.0
19	1174.0
20	1162.0
21	1240.5
22	1319.0
23	1217.5
24	1116.0
25	1120.5
26	1244.0
27	1363.0
28	2067.5
29	2772.0
30	3012.0
31	3252.0
32	3788.0
33	4324.0
34	4439.0
35	4554.0
36	5841.5
37	7129.0
38	7551.0
39	7973.0
40	10473.5
41	12974.0
42	18969.0
43	24964.0
44	33326.5
45	41689.0
46	40772.5
47	39856.0
48	39079.5
49	38303.0
50	35877.0
51	33451.0
52	30585.0
53	27719.0
54	24198.0
55	20677.0
56	19152.5
57	17628.0
58	16051.0
59	14474.0
60	13514.0
61	12554.0
62	11621.5
63	10689.0
64	8967.5
65	7246.0
66	6334.0
67	5422.0
68	4333.5
69	3245.0
70	2872.0
71	2499.0
72	2059.0
73	1619.0
74	1468.5
75	1090.0
76	862.0
77	709.5
78	557.0
79	420.5
80	284.0
81	253.5
82	223.0
83	185.0
84	147.0
85	114.0
86	81.0
87	67.5
88	54.0
89	64.0
90	74.0
91	40.0
92	6.0
93	3.5
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	368085.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.511224493420855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.36374001297942	22.05734959160702
2	10.10146245644966	5.760101276153975
3	3.381590140688832	2.892398269377537
4	1.5992915445373745	1.8239104102693946
5	0.9012463658441409	1.2847818730231004
6	0.592115602901432	1.012916452822798
7	0.40869038407771063	0.8156584302119401
8	0.33293188560158654	0.7593836585123795
9	0.2561779606820356	0.6573552612545032
>10	3.6572638583402943	25.90741035889294
>50	1.2430111812114792	24.305089347315157
>100	0.14852338893384956	6.090411299130785
>500	0.007974410144099307	1.5326919335610043
>1k	0.005980807608074479	5.1005418378674845
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGCT	3628	0.985641903364712	Illumina Single End Adapter 2 (95% over 22bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCC	3590	0.9753182009590177	No Hit
AATCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCT	3434	0.9329366858198515	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGT	3135	0.8517054484697828	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2886	0.7840580300745751	No Hit
AAACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCT	1274	0.3461157069698575	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTT	977	0.26542782237798335	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	756	0.20538734259749786	No Hit
AATGACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTT	730	0.19832375674097014	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	716	0.19452028743360908	No Hit
AACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTG	594	0.1613757691837483	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCT	586	0.15920235815097056	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCT	521	0.1415433935096513	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	513	0.13936998247687354	No Hit
AGCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCTCTACAGGTC	458	0.12442778162652648	No Hit
CGCAGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	390	0.10595378784791556	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTAT	389	0.10568211146881835	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.3070350598367224	0.0
2	0.0	0.0	0.0	1.6015322547781083	0.0
3	0.0	0.0	0.0	2.0837578276756727	0.0
4	0.0	0.0	0.0	4.7975331784777975	0.0
5	0.0	0.0	0.0	5.195267397476126	0.0
6	0.0	2.716763790972194E-4	0.0	6.923129168534442	0.0
7	0.0	2.716763790972194E-4	0.0	8.281782740399636	0.0
8	0.0	2.716763790972194E-4	0.0	9.096540201312196	0.0
9	0.0	2.716763790972194E-4	0.0	11.107760435768911	0.0
10	0.0	2.716763790972194E-4	0.0	12.862518168357852	0.0
11	0.0	2.716763790972194E-4	0.0	15.71186003232949	0.0
12	0.0	2.716763790972194E-4	0.0	16.634473015743644	0.0
13	0.0	2.716763790972194E-4	0.0	17.140606110001766	0.0
14	0.0	2.716763790972194E-4	0.0	17.63369873806322	0.0
15	0.0	2.716763790972194E-4	0.0	17.93661790075662	0.0
16	0.0	2.716763790972194E-4	0.0	18.584566064903488	0.0
17	0.0	2.716763790972194E-4	0.0	19.21947376285369	0.0
18	0.0	2.716763790972194E-4	0.0	20.389583927625413	0.0
19	0.0	2.716763790972194E-4	0.0	20.743034896830896	0.0
20	0.0	2.716763790972194E-4	0.0	21.24373446350707	0.0
21	0.0	2.716763790972194E-4	0.0	21.628971569066927	0.0
22	0.0	2.716763790972194E-4	0.0	22.045451458222963	0.0
23	0.0	2.716763790972194E-4	0.0	22.45350937962699	0.0
24	0.0	2.716763790972194E-4	0.0	22.71024355787386	0.0
25	0.0	2.716763790972194E-4	0.0	22.938723392694623	0.0
26	0.0	2.716763790972194E-4	0.0	23.16665987475719	0.0
27	0.0	2.716763790972194E-4	0.0	23.446758221606423	0.0
28	0.0	2.716763790972194E-4	0.0	23.678226496597254	0.0
29	0.0	2.716763790972194E-4	0.0	23.89448089435864	0.0
30	0.0	2.716763790972194E-4	0.0	24.29601858266433	0.0
31	0.0	2.716763790972194E-4	0.0	24.552752760911204	0.0
32	0.0	2.716763790972194E-4	0.0	24.79644647296141	0.0
33	0.0	2.716763790972194E-4	0.0	25.053724003966476	0.0
34	0.0	2.716763790972194E-4	0.0	25.252047760707445	0.0
35	0.0	2.716763790972194E-4	0.0	25.513672113778068	0.0
36	0.0	2.716763790972194E-4	0.0	25.73617506825869	0.0
37	0.0	2.716763790972194E-4	0.0	25.95786299360202	0.0
38	0.0	2.716763790972194E-4	0.0	26.192319708762923	0.0
39	0.0	2.716763790972194E-4	0.0	26.462637705964656	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTTAT	25	3.8856204E-5	45.000004	44
GTCGCAA	25	3.8856204E-5	45.000004	12
CAAGTTG	25	3.8856204E-5	45.000004	1
GTATTCG	25	3.8856204E-5	45.000004	9
CACGGTA	25	3.8856204E-5	45.000004	31
CGAAACG	25	3.8856204E-5	45.000004	1
CAACGAA	25	3.8856204E-5	45.000004	23
TAGACAG	25	3.8856204E-5	45.000004	1
AGCCCGA	25	3.8856204E-5	45.000004	2
TAGAATA	25	3.8856204E-5	45.000004	32
GACAATC	30	2.161465E-6	45.000004	9
ATTACTA	30	2.161465E-6	45.000004	24
TTAGAAT	25	3.8856204E-5	45.000004	31
CGTTGAT	30	2.161465E-6	45.000004	25
TTCACCG	30	2.161465E-6	45.000004	1
AAGACGC	25	3.8856204E-5	45.000004	39
GCACGGT	25	3.8856204E-5	45.000004	30
TGAACGG	25	3.8856204E-5	45.000004	2
ATTCGTT	25	3.8856204E-5	45.000004	11
CGCGATA	25	3.8856204E-5	45.000004	11
>>END_MODULE