FastQCFastQC Report
Sat 18 Jun 2016
SRR3548810_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548810_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences364224
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCC43271.1880051836232648No Hit
AATCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCT39571.0864193463363205No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGT35260.96808557371288No Hit
CTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGCT28010.7690322438938675No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22990.6312049727640133No Hit
AAACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCT9980.2740072043577579No Hit
AATGACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTT8920.24490423475663328No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTT7910.21717404674046742No Hit
AATGATCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCT7280.19987699876998768No Hit
AACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTG5790.15896810753821825No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5330.14633851695659814No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA5170.1419456158847303No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTAT4440.12190300474433316No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4440.12190300474433316No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCT4300.11805921630644878No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGG351.208864E-745.0000042
GCTTGCG351.208864E-745.0000041
CCTTCGT253.885541E-545.032
GTTTGAC207.0269336E-445.029
AACCGTT207.0269336E-445.016
TTAGGAT253.885541E-545.039
TAATAGG800.045.02
ACTATCG253.885541E-545.01
GTACCTT207.0269336E-445.07
AATCCGA253.885541E-545.027
CTCACCG207.0269336E-445.01
GCCCACG207.0269336E-445.01
TACCCCC207.0269336E-445.024
ACCGGGT750.045.04
CTACTTG207.0269336E-445.01
GTCGATA207.0269336E-445.044
GGTAATC253.885541E-545.08
GATCGCG207.0269336E-445.09
ATAGGCG207.0269336E-445.01
GTTTCGA253.885541E-545.023