##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548810_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	364224
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.372136377613778	31.0	31.0	34.0	30.0	34.0
2	31.559315146722895	31.0	31.0	34.0	30.0	34.0
3	31.733438762959057	31.0	31.0	34.0	30.0	34.0
4	35.53582410824108	37.0	35.0	37.0	33.0	37.0
5	35.1195692760499	37.0	35.0	37.0	32.0	37.0
6	35.133519482516256	37.0	35.0	37.0	32.0	37.0
7	35.09194067387102	37.0	35.0	37.0	32.0	37.0
8	35.41943693990511	37.0	35.0	37.0	33.0	37.0
9	36.35903180460376	39.0	37.0	39.0	32.0	39.0
10	36.08349257599719	38.0	35.0	39.0	31.0	39.0
11	36.271368718151464	38.0	35.0	39.0	32.0	39.0
12	35.73589329643296	37.0	35.0	39.0	31.0	39.0
13	35.44613479616939	37.0	35.0	39.0	30.0	39.0
14	36.41584574327886	38.0	35.0	40.0	31.0	41.0
15	36.48064927077842	38.0	35.0	40.0	31.0	41.0
16	36.513085354067826	38.0	35.0	40.0	31.0	41.0
17	36.47069660428747	38.0	35.0	40.0	31.0	41.0
18	36.27260422157793	38.0	35.0	40.0	30.0	41.0
19	36.24299606835354	38.0	34.0	40.0	30.0	41.0
20	36.2606692584783	38.0	34.0	40.0	30.0	41.0
21	36.307055548234054	38.0	35.0	40.0	31.0	41.0
22	36.3126372781585	38.0	35.0	40.0	31.0	41.0
23	36.258417896678964	38.0	35.0	40.0	31.0	41.0
24	35.92559523809524	38.0	34.0	40.0	30.0	41.0
25	36.01121287998594	38.0	34.0	40.0	30.0	41.0
26	35.86969282639255	38.0	34.0	40.0	30.0	41.0
27	35.80840911087682	38.0	34.0	40.0	30.0	41.0
28	35.70274336671938	38.0	34.0	40.0	30.0	41.0
29	35.41571121068353	37.0	34.0	40.0	29.0	41.0
30	35.461861382006674	37.0	34.0	40.0	29.0	41.0
31	35.08597181953962	37.0	34.0	40.0	27.0	41.0
32	35.12225992795642	37.0	34.0	40.0	27.0	41.0
33	35.06567936215077	37.0	34.0	40.0	27.0	41.0
34	34.785747232472325	37.0	34.0	40.0	26.0	41.0
35	34.817985635213496	37.0	33.0	40.0	26.0	41.0
36	34.3716504129327	36.0	33.0	40.0	24.0	41.0
37	34.4201315673871	36.0	33.0	40.0	25.0	41.0
38	34.313694319978914	36.0	33.0	40.0	24.0	41.0
39	34.27512739413108	36.0	33.0	40.0	24.0	41.0
40	34.177432019855914	36.0	33.0	40.0	24.0	41.0
41	34.1748868827974	36.0	33.0	40.0	24.0	41.0
42	34.132067079599366	36.0	33.0	40.0	24.0	41.0
43	33.99517879107363	36.0	33.0	39.0	23.0	41.0
44	33.87400336056932	35.0	33.0	39.0	23.0	41.0
45	33.60534725882973	35.0	33.0	39.0	23.0	41.0
46	33.482667259708315	35.0	32.0	39.0	23.0	40.0
47	33.38581202776314	35.0	32.0	39.0	22.0	40.0
48	33.38663295115094	35.0	32.0	39.0	23.0	40.0
49	33.28444858109295	35.0	32.0	39.0	22.0	40.0
50	33.00230352749956	35.0	32.0	39.0	20.0	40.0
51	31.091797904586187	34.0	28.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	2.0
11	15.0
12	13.0
13	18.0
14	31.0
15	55.0
16	82.0
17	203.0
18	406.0
19	734.0
20	1112.0
21	1688.0
22	2269.0
23	2946.0
24	3599.0
25	4223.0
26	4961.0
27	5790.0
28	6854.0
29	8721.0
30	10818.0
31	13974.0
32	18043.0
33	24717.0
34	40691.0
35	41387.0
36	34730.0
37	44547.0
38	54425.0
39	37163.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.803747144614302	36.66781980319803	18.644295817958177	24.884137234229485
2	22.794489105605344	34.85602266736953	19.359240467404675	22.990247759620452
3	19.913569671411	31.030354946406607	23.87843744508874	25.177637937093657
4	21.92332191179055	33.714417501317875	18.20720216130733	26.155058425584254
5	16.814927077842206	37.62135389211035	19.295543401862588	26.268175628184853
6	20.10328808645229	37.01348620629064	21.385191530486733	21.49803417677034
7	76.72943024073098	10.869959146020031	7.124461869618696	5.276148743630293
8	78.82182393252504	6.664854594974521	5.521052978386927	8.992268494113514
9	74.3984471094711	10.017736338077667	9.64928176067475	5.934534791776489
10	44.54044763661923	27.420763046916186	12.485997627833422	15.55279168863117
11	37.8357823756809	24.031090757336145	20.8077995958531	17.325327271129854
12	34.76157529432437	23.513826656123705	26.099598049551926	15.625
13	25.940355385696712	33.625461254612546	21.268779652082234	19.165403707608505
14	17.345918994904235	33.90825426111404	29.86294148655772	18.882885257424004
15	15.53631830961167	28.405596555965563	34.09275610613249	21.965329028290284
16	17.844238710244245	25.53950316288877	34.788756369706554	21.827501757160427
17	17.523007819363908	27.125340449833068	27.28156299420137	28.07008873660165
18	23.366389913899138	26.05319803198032	26.22809040590406	24.35232164821648
19	23.85729660868037	30.40079731154454	25.316288877174486	20.425617202600595
20	27.474850641363556	30.061720260059744	21.93842250922509	20.52500658935161
21	21.30611931119311	31.40430065014936	23.14921586715867	24.140364171498856
22	20.44044324371815	30.396404410472677	24.455554823405375	24.707597522403795
23	22.846654805833772	30.305251713231417	21.511761992619927	25.336331488314883
24	19.42513398348269	30.897195132665612	27.086078896503253	22.591591987348444
25	20.76167413459849	27.857582147250042	25.35637409945528	26.024369618696184
26	22.862853628536286	31.682975311895976	22.85873528378141	22.595435775786328
27	20.189498769987697	29.00962045334739	25.744047619047617	25.05683315761729
28	22.852695044807593	29.201809875241608	27.237908539799683	20.707586540151116
29	20.06347742048849	29.312456070989278	29.469502284308557	21.154564224213672
30	26.580895273238447	26.612743806009487	23.717272887014584	23.089088033737482
31	27.72414777719206	31.114918292040063	20.77485283781409	20.386081092953788
32	28.36715867158672	24.94179406079775	24.962934897206114	21.72811237040942
33	28.64171498857846	26.5325733614479	21.252855385696716	23.57285626427693
34	21.806360920752066	28.923409769811986	26.02491873133017	23.245310578105784
35	27.806789228606572	25.17489237392374	25.724005007907223	21.294313389562465
36	25.525500790722194	28.735887805306625	27.2538328061852	18.48477859778598
37	25.438740994552806	30.14243981725532	22.7027872957301	21.716031892461782
38	24.607933579335793	30.656958355297835	23.17914250571077	21.555965559655597
39	22.886740028114566	27.858680372518013	26.050452468810402	23.20412713055702
40	26.31924310314532	26.456521261641186	21.621859075733614	25.60237655947988
41	21.40715603584607	25.4521942540854	26.921070549991217	26.219579160077316
42	29.171334124055527	24.712814092426637	20.973906167633103	25.14194561588473
43	23.880908451941664	24.540392725355826	25.715493762080477	25.863205060622036
44	22.76758258654015	25.035143208574944	24.526390353189246	27.67088385169566
45	26.19377086628009	27.332905025478826	23.370508258654016	23.102815849587067
46	20.96841504129327	25.476904322614658	30.46751449657354	23.08716613951854
47	23.145646635037778	26.774732033034613	23.709310753821825	26.370310578105784
48	23.115994552802672	21.657825953259533	31.61735635213495	23.608823141802844
49	24.25265770514848	20.83305877701634	28.849554120541203	26.064729397293974
50	19.811434721490073	25.708080741521698	25.192738534528203	29.287746002460025
51	20.774029168863116	24.118399666139517	29.15293885081708	25.954632314180287
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2731.0
1	2206.5
2	1682.0
3	1187.0
4	692.0
5	689.5
6	687.0
7	719.5
8	752.0
9	811.0
10	870.0
11	901.5
12	933.0
13	957.5
14	982.0
15	1043.0
16	1104.0
17	1040.0
18	976.0
19	950.5
20	925.0
21	941.0
22	957.0
23	990.5
24	1024.0
25	1223.0
26	1789.0
27	2156.0
28	2232.0
29	2308.0
30	2781.5
31	3255.0
32	3822.5
33	4390.0
34	4861.0
35	5332.0
36	6419.0
37	7506.0
38	8136.5
39	8767.0
40	11698.5
41	14630.0
42	18325.5
43	22021.0
44	30569.5
45	39118.0
46	38106.0
47	37094.0
48	35801.0
49	34508.0
50	32284.5
51	30061.0
52	27650.0
53	25239.0
54	23359.5
55	21480.0
56	19794.0
57	18108.0
58	16682.0
59	15256.0
60	14709.5
61	14163.0
62	13190.0
63	12217.0
64	11286.5
65	10356.0
66	8522.0
67	6688.0
68	5299.0
69	3910.0
70	3442.5
71	2975.0
72	2679.5
73	2384.0
74	2111.0
75	1537.0
76	1236.0
77	909.5
78	583.0
79	545.0
80	507.0
81	345.0
82	183.0
83	147.5
84	112.0
85	79.5
86	47.0
87	33.5
88	20.0
89	18.0
90	16.0
91	13.0
92	10.0
93	8.5
94	7.0
95	6.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	364224.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.457898905033733
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.7352743035322	22.12182577484986
2	9.444640520557018	5.375492502567936
3	3.072491113596366	2.6230992449201973
4	1.463710093510052	1.6661645546954613
5	0.8753390360727682	1.2455154898094258
6	0.6078182738719504	1.0378338594288044
7	0.40421148133247153	0.8052106630409385
8	0.35031629614473664	0.797541259237822
9	0.29043313128345966	0.7438605019863378
>10	4.479257271993261	31.223962855586258
>50	1.1687188646299922	22.1323346858834
>100	0.09581298975617357	3.9933861927457603
>500	0.006986363836387656	1.4309160482968093
>1k	0.00499025988313404	4.802856366950982
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCC	4327	1.1880051836232648	No Hit
AATCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCT	3957	1.0864193463363205	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGT	3526	0.96808557371288	No Hit
CTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTGCT	2801	0.7690322438938675	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2299	0.6312049727640133	No Hit
AAACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCT	998	0.2740072043577579	No Hit
AATGACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTT	892	0.24490423475663328	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTT	791	0.21717404674046742	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCT	728	0.19987699876998768	No Hit
AACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCTTCTG	579	0.15896810753821825	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	533	0.14633851695659814	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	517	0.1419456158847303	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTAT	444	0.12190300474433316	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	444	0.12190300474433316	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGTGATCAGTCGTATGCCGTCT	430	0.11805921630644878	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.491126339834827E-4	0.0	0.0	1.085870233702337	0.0
2	5.491126339834827E-4	0.0	0.0	1.3678395712528555	0.0
3	5.491126339834827E-4	0.0	0.0	1.8570989281321384	0.0
4	5.491126339834827E-4	0.0	0.0	4.689971006852925	0.0
5	5.491126339834827E-4	0.0	0.0	5.04689421894219	0.0
6	5.491126339834827E-4	0.0	0.0	6.71043094359515	0.0
7	5.491126339834827E-4	0.0	0.0	8.007434985064137	0.0
8	5.491126339834827E-4	0.0	0.0	8.831653048673344	0.0
9	5.491126339834827E-4	0.0	0.0	10.974015990159902	0.0
10	5.491126339834827E-4	0.0	0.0	12.509060358460728	0.0
11	5.491126339834827E-4	0.0	0.0	15.419082762256194	0.0
12	5.491126339834827E-4	0.0	0.0	16.217217975751186	0.0
13	5.491126339834827E-4	0.0	0.0	16.62932700755579	0.0
14	5.491126339834827E-4	0.0	0.0	17.126273941310842	0.0
15	5.491126339834827E-4	0.0	0.0	17.414008961518185	0.0
16	5.491126339834827E-4	0.0	0.0	17.966141714988577	0.0
17	5.491126339834827E-4	0.0	0.0	18.544357318573187	0.0
18	5.491126339834827E-4	0.0	0.0	19.41113161131611	0.0
19	5.491126339834827E-4	0.0	0.0	19.738128184853277	0.0
20	5.491126339834827E-4	0.0	0.0	20.106308205939204	0.0
21	5.491126339834827E-4	0.0	0.0	20.468173431734318	0.0
22	5.491126339834827E-4	0.0	0.0	20.833607889650324	0.0
23	5.491126339834827E-4	0.0	0.0	21.190531101739587	0.0
24	5.491126339834827E-4	0.0	0.0	21.44449569495695	0.0
25	5.491126339834827E-4	0.0	0.0	21.673475663328063	0.0
26	5.491126339834827E-4	0.0	0.0	21.89394438587243	0.0
27	5.491126339834827E-4	0.0	0.0	22.140495958531012	0.0
28	5.491126339834827E-4	0.0	0.0	22.356571780003513	0.0
29	5.491126339834827E-4	0.0	0.0	22.585277192057635	0.0
30	5.491126339834827E-4	0.0	0.0	22.989424090669477	0.0
31	5.491126339834827E-4	0.0	0.0	23.23707388859603	0.0
32	5.491126339834827E-4	0.0	0.0	23.464955631699173	0.0
33	5.491126339834827E-4	0.0	0.0	23.706016078017925	0.0
34	5.491126339834827E-4	0.0	0.0	23.938565278509927	0.0
35	5.491126339834827E-4	0.0	0.0	24.21833816552451	0.0
36	5.491126339834827E-4	0.0	0.0	24.42919741697417	0.0
37	5.491126339834827E-4	0.0	0.0	24.669983306975926	0.0
38	5.491126339834827E-4	0.0	0.0	24.90555262695484	0.0
39	5.491126339834827E-4	0.0	0.0	25.16226278334212	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGG	35	1.208864E-7	45.000004	2
GCTTGCG	35	1.208864E-7	45.000004	1
CCTTCGT	25	3.885541E-5	45.0	32
GTTTGAC	20	7.0269336E-4	45.0	29
AACCGTT	20	7.0269336E-4	45.0	16
TTAGGAT	25	3.885541E-5	45.0	39
TAATAGG	80	0.0	45.0	2
ACTATCG	25	3.885541E-5	45.0	1
GTACCTT	20	7.0269336E-4	45.0	7
AATCCGA	25	3.885541E-5	45.0	27
CTCACCG	20	7.0269336E-4	45.0	1
GCCCACG	20	7.0269336E-4	45.0	1
TACCCCC	20	7.0269336E-4	45.0	24
ACCGGGT	75	0.0	45.0	4
CTACTTG	20	7.0269336E-4	45.0	1
GTCGATA	20	7.0269336E-4	45.0	44
GGTAATC	25	3.885541E-5	45.0	8
GATCGCG	20	7.0269336E-4	45.0	9
ATAGGCG	20	7.0269336E-4	45.0	1
GTTTCGA	25	3.885541E-5	45.0	23
>>END_MODULE