FastQCFastQC Report
Sat 18 Jun 2016
SRR3548805_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548805_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367192
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15770.4294755877034358No Hit
CTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGCT15750.4289309135275279Illumina Single End Adapter 2 (95% over 23bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCC13670.37228479923309876No Hit
AATCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCT10910.2971197629577987No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGT9010.2453757162465413No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG6540.1781084555219068No Hit
AAACTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCT6220.16939366870737924No Hit
CTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCTGTCTCTTAT5520.1503300725506002No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTT4680.1274537571624654No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA3960.10784548682977843No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3690.10049238545502082No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG453.8380676E-1045.0000041
ACGCATG351.2089004E-745.0000041
ATAGCGG453.8380676E-1045.0000042
CCTCGCG351.2089004E-745.0000041
AATGCGG453.8380676E-1045.0000042
ATAAGCG351.2089004E-745.00000433
TTTGCGC453.8380676E-1045.00000413
CCGCACG351.2089004E-745.0000041
AGGTACG207.0270087E-445.024
ACTATCC207.0270087E-445.043
AACCGCG253.8856022E-545.01
TCGTTGT207.0270087E-445.035
GCCTTGT207.0270087E-445.045
GATACAC207.0270087E-445.030
ACCGGTA253.8856022E-545.029
ACACGTA207.0270087E-445.025
TCGCACG253.8856022E-545.01
CGAACCG253.8856022E-545.028
TCCGCGA406.7939254E-945.033
TCCGCAC207.0270087E-445.08