##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548805_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	367192
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26946937841783	31.0	31.0	34.0	28.0	34.0
2	31.48205026253295	31.0	31.0	34.0	30.0	34.0
3	31.456943506394474	31.0	31.0	34.0	28.0	34.0
4	35.47443571755376	37.0	35.0	37.0	33.0	37.0
5	35.158181550796314	37.0	35.0	37.0	32.0	37.0
6	35.217510185407086	37.0	35.0	37.0	32.0	37.0
7	35.16186082485457	37.0	35.0	37.0	32.0	37.0
8	35.523216736748076	37.0	35.0	37.0	33.0	37.0
9	35.902546896446545	39.0	37.0	39.0	32.0	39.0
10	35.81615067866402	38.0	35.0	39.0	30.0	39.0
11	36.21088694742805	38.0	35.0	39.0	32.0	39.0
12	35.82133870018955	37.0	35.0	39.0	31.0	39.0
13	35.45295921479771	37.0	35.0	39.0	30.0	39.0
14	36.47993965010131	38.0	35.0	40.0	31.0	41.0
15	36.6067506917362	38.0	35.0	40.0	31.0	41.0
16	36.69808982766509	38.0	35.0	40.0	32.0	41.0
17	36.659818296694915	38.0	35.0	40.0	32.0	41.0
18	36.55027614980718	38.0	35.0	40.0	31.0	41.0
19	36.57291825529968	38.0	35.0	40.0	31.0	41.0
20	36.482240898494524	38.0	35.0	40.0	31.0	41.0
21	36.48432700058825	38.0	35.0	40.0	31.0	41.0
22	36.463378831782826	38.0	35.0	40.0	31.0	41.0
23	36.39470086494259	38.0	35.0	40.0	31.0	41.0
24	35.99626898189503	38.0	34.0	40.0	30.0	41.0
25	36.10115144120787	38.0	35.0	40.0	30.0	41.0
26	36.0241590230724	38.0	34.0	40.0	30.0	41.0
27	35.92402612257348	38.0	34.0	40.0	30.0	41.0
28	35.8062376086625	38.0	34.0	40.0	30.0	41.0
29	35.598054968517836	37.0	34.0	40.0	29.0	41.0
30	35.53584228414562	37.0	34.0	40.0	29.0	41.0
31	35.45110732259962	37.0	34.0	40.0	29.0	41.0
32	35.34104773524478	37.0	34.0	40.0	29.0	41.0
33	35.189385934334084	37.0	34.0	40.0	27.0	41.0
34	35.18367230222881	37.0	34.0	40.0	27.0	41.0
35	35.03023758687553	37.0	34.0	40.0	27.0	41.0
36	34.863534608597135	37.0	34.0	40.0	26.0	41.0
37	34.72928059434846	37.0	33.0	40.0	26.0	41.0
38	34.641835878777314	37.0	33.0	40.0	26.0	41.0
39	34.52350813743219	36.0	33.0	40.0	25.0	41.0
40	34.35476535436502	36.0	33.0	40.0	24.0	41.0
41	34.38138085797076	36.0	33.0	40.0	24.0	41.0
42	34.35287533497462	36.0	33.0	40.0	24.0	41.0
43	34.28094838667509	36.0	33.0	40.0	24.0	41.0
44	34.2533279592148	36.0	33.0	40.0	24.0	41.0
45	34.09429944007495	36.0	33.0	40.0	24.0	41.0
46	33.94092463888102	35.0	33.0	39.0	23.0	41.0
47	33.78522407895597	35.0	33.0	39.0	23.0	41.0
48	33.746465064598354	35.0	33.0	39.0	23.0	41.0
49	33.56634131462559	35.0	33.0	39.0	23.0	40.0
50	33.42951916163751	35.0	32.0	39.0	23.0	40.0
51	31.67328264232336	34.0	29.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	3.0
12	4.0
13	18.0
14	28.0
15	54.0
16	76.0
17	191.0
18	359.0
19	618.0
20	1140.0
21	1618.0
22	2200.0
23	2812.0
24	3416.0
25	3932.0
26	4500.0
27	5345.0
28	6512.0
29	8050.0
30	9929.0
31	13308.0
32	17232.0
33	24198.0
34	40059.0
35	42134.0
36	35977.0
37	46778.0
38	56593.0
39	40101.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.443528181441863	32.273034270899146	20.968594087017145	26.314843460641846
2	23.753513148434607	32.29563824919933	20.143140373428615	23.80770822893745
3	20.95770060349899	32.578868820671474	20.24254341053182	26.220887165297718
4	21.50400880193468	33.468594087017145	18.374310987167476	26.653086123880694
5	17.467973158456612	36.35128216301008	17.68856619969934	28.492178478833964
6	20.553824702063224	38.19990631604174	19.29263164774832	21.953637334146713
7	81.45629534412514	8.121364299875815	5.452460837926752	4.969879518072289
8	84.02171071265168	6.311412013333624	4.203795289657727	5.463081984356958
9	75.97469443778732	12.048192771084338	7.7564326020174725	4.220680189110873
10	46.12954530599795	27.448310420706335	11.808263796596876	14.61388047669884
11	37.29247913897906	25.880193468267283	20.548650297392097	16.278677095361555
12	35.59990413734504	23.294352818144187	24.57079674938452	16.534946295126254
13	25.49075143249308	34.11239896294037	21.354223403559992	19.042626201006556
14	18.98352905292054	35.18104969607181	27.18005838907166	18.65536286193599
15	16.990838580361228	27.838297130656443	35.28481012658228	19.88605416240005
16	18.339179502821413	24.218120220484106	34.80740321139894	22.63529706529554
17	17.123466742194818	25.26362230113946	28.158293209002377	29.45461774766335
18	22.199830061657117	25.82981110699579	26.940129414584195	25.030229416762893
19	24.25461339026994	31.105797511928362	23.739079282773044	20.900509815028652
20	27.004400967341336	28.774047364866334	22.529085600993486	21.69246606679884
21	22.780452733175014	30.86423451491318	22.99614370683457	23.359169045077234
22	20.924203141680646	28.384060654916226	23.454759362949083	27.23697684045404
23	22.026623673718383	29.960892394169807	21.502374779406956	26.510109152704853
24	19.907568792348417	29.286313427307785	27.91809189742696	22.88802588291684
25	19.53882437525872	28.529488659883658	25.75546308198436	26.176223882873266
26	22.087082507244165	32.09547048955315	22.762750822458006	23.05469618074468
27	18.288797141549924	31.122682411381515	24.930554042571735	25.65796640449683
28	19.78202139480163	29.71088694742805	28.857110176692302	21.64998148107802
29	21.821826183576984	28.179807838950737	28.0403712499183	21.957994727553977
30	22.171779341597855	31.16761803089392	24.05934769820693	22.60125492930129
31	29.324440619621345	28.785485522560407	19.07557898864899	22.814494869169263
32	29.0997625220593	28.65149567528704	20.045643695941088	22.203098106712567
33	26.051765833678292	29.579348133946276	21.149698250506546	23.219187781868886
34	23.846924769602822	28.727750059914158	22.538889736159827	24.886435434323186
35	22.718359877121504	30.75366565720386	24.255702738621757	22.27227172705288
36	28.659665787925665	28.331227259853154	23.708849866010155	19.30025708621103
37	24.489367960086277	32.78067060284538	21.231944051068652	21.498017385999695
38	23.90656659186475	31.03444519488442	22.359147258110198	22.699840955140633
39	24.681365607093834	29.29230484324277	23.153554543671977	22.872775005991418
40	24.994825595328873	28.72720538573825	21.78260964291161	24.495359376021263
41	21.475958081875422	24.510610252946687	26.744591385433235	27.268840279744655
42	25.769079936382056	26.48804984858058	21.39943136016035	26.34343885487701
43	22.65245430183664	25.91723131222902	25.295758077518027	26.134556308416308
44	22.41388701278895	26.99704786596658	24.82216388156605	25.766901239678425
45	22.821575633456067	28.93690494346282	24.518780365585307	23.722739057495808
46	23.022288067278154	27.214100525065906	27.68715004684198	22.076461360813962
47	22.632029020240093	27.849462951262556	25.457253970674742	24.06125405782261
48	24.659578640057518	21.589522647552233	29.263981786095556	24.48691692629469
49	21.37873374147585	23.413091788492125	29.01343166517789	26.194742804854137
50	20.976491862567812	26.26663979607399	26.458365105993593	26.298503235364606
51	21.42938843983529	27.055872676964636	26.949661212662583	24.565077670537487
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1977.0
1	1630.5
2	1284.0
3	964.5
4	645.0
5	648.5
6	652.0
7	714.0
8	776.0
9	848.0
10	920.0
11	1001.5
12	1083.0
13	1143.0
14	1203.0
15	1181.0
16	1159.0
17	1097.0
18	1035.0
19	1029.5
20	1024.0
21	1034.5
22	1045.0
23	1207.5
24	1370.0
25	1378.0
26	1523.5
27	1661.0
28	2222.0
29	2783.0
30	2929.0
31	3075.0
32	4096.0
33	5117.0
34	6137.5
35	7158.0
36	8103.5
37	9049.0
38	9181.0
39	9313.0
40	11802.0
41	14291.0
42	18353.0
43	22415.0
44	28637.5
45	34860.0
46	35129.0
47	35398.0
48	34437.0
49	33476.0
50	32621.0
51	31766.0
52	28739.0
53	25712.0
54	23223.0
55	20734.0
56	19201.5
57	17669.0
58	16948.0
59	16227.0
60	15852.0
61	15477.0
62	13712.5
63	11948.0
64	10954.0
65	9960.0
66	8509.5
67	7059.0
68	6018.5
69	4978.0
70	4284.0
71	3590.0
72	3055.0
73	2520.0
74	2200.5
75	1625.5
76	1370.0
77	1150.0
78	930.0
79	681.5
80	433.0
81	380.5
82	328.0
83	280.5
84	233.0
85	164.5
86	96.0
87	70.5
88	45.0
89	42.0
90	39.0
91	35.5
92	32.0
93	20.0
94	8.0
95	5.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	367192.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.86377247292443
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.92925790570214	22.2046859670111
2	9.81896705000913	5.66824861701211
3	3.367705073197059	2.916140189660197
4	1.6177376675669874	1.8677604783013195
5	0.9104358585725985	1.3139306736515544
6	0.5845620997286569	1.0123600465717748
7	0.4102680308963539	0.8289318167694965
8	0.32661783184342635	0.754193822714284
9	0.30869305591834856	0.8019041516999056
>10	4.313734357245698	31.144044589414356
>50	1.3204089830167762	25.377523044091355
>100	0.08364581792866455	3.713475408630941
>500	0.003983134187079264	0.7843710828292445
>1k	0.003983134187079264	1.6124301116423823
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1577	0.4294755877034358	No Hit
CTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGCT	1575	0.4289309135275279	Illumina Single End Adapter 2 (95% over 23bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCC	1367	0.37228479923309876	No Hit
AATCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCT	1091	0.2971197629577987	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGT	901	0.2453757162465413	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	654	0.1781084555219068	No Hit
AAACTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCT	622	0.16939366870737924	No Hit
CTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCTGTCTCTTAT	552	0.1503300725506002	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTT	468	0.1274537571624654	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	396	0.10784548682977843	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	369	0.10049238545502082	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7290463844528203	0.0
2	0.0	0.0	0.0	0.9338438745942177	0.0
3	0.0	0.0	0.0	1.2219765136495349	0.0
4	0.0	0.0	0.0	2.767489487788405	0.0
5	0.0	0.0	0.0	3.057256149371446	0.0
6	0.0	0.0	0.0	4.388439835290529	0.0
7	0.0	0.0	0.0	5.435031264297697	0.0
8	0.0	0.0	0.0	6.284450641626179	0.0
9	0.0	0.0	0.0	7.5312098302795265	0.0
10	0.0	0.0	0.0	9.093607703871545	0.0
11	0.0	0.0	0.0	10.896751563214885	0.0
12	0.0	0.0	0.0	11.670461230092158	0.0
13	0.0	0.0	0.0	12.071069086472471	0.0
14	0.0	0.0	0.0	12.429192357131964	0.0
15	0.0	0.0	0.0	12.670210679971241	0.0
16	0.0	0.0	0.0	13.099686267674677	0.0
17	0.0	0.0	0.0	13.60269286912569	0.0
18	0.0	0.0	0.0	14.288437656593825	0.0
19	0.0	0.0	0.0	14.566493823394845	0.0
20	0.0	0.0	0.0	14.85653282206584	0.0
21	0.0	0.0	0.0	15.170265147388832	0.0
22	0.0	0.0	0.0	15.50632911392405	0.0
23	0.0	0.0	0.0	15.872077823046254	0.0
24	0.0	0.0	0.0	16.132704416218218	0.0
25	5.446741759079719E-4	0.0	0.0	16.362556918451382	0.0
26	5.446741759079719E-4	0.0	0.0	16.54883548661191	0.0
27	5.446741759079719E-4	0.0	0.0	16.771879561646223	0.0
28	5.446741759079719E-4	0.0	0.0	16.971502647116495	0.0
29	5.446741759079719E-4	0.0	0.0	17.175755463081984	0.0
30	5.446741759079719E-4	0.0	0.0	17.49874724939541	0.0
31	5.446741759079719E-4	0.0	0.0	17.724514695309267	0.0
32	5.446741759079719E-4	0.0	0.0	17.9557288829822	0.0
33	5.446741759079719E-4	0.0	0.0	18.20518965554805	0.0
34	5.446741759079719E-4	0.0	0.0	18.439127214100527	0.0
35	5.446741759079719E-4	0.0	0.0	18.70465587485566	0.0
36	5.446741759079719E-4	0.0	0.0	18.926882938626115	0.0
37	5.446741759079719E-4	0.0	0.0	19.144752608989304	0.0
38	5.446741759079719E-4	0.0	0.0	19.377328482102005	0.0
39	5.446741759079719E-4	0.0	0.0	19.610721366478572	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACTG	45	3.8380676E-10	45.000004	1
ACGCATG	35	1.2089004E-7	45.000004	1
ATAGCGG	45	3.8380676E-10	45.000004	2
CCTCGCG	35	1.2089004E-7	45.000004	1
AATGCGG	45	3.8380676E-10	45.000004	2
ATAAGCG	35	1.2089004E-7	45.000004	33
TTTGCGC	45	3.8380676E-10	45.000004	13
CCGCACG	35	1.2089004E-7	45.000004	1
AGGTACG	20	7.0270087E-4	45.0	24
ACTATCC	20	7.0270087E-4	45.0	43
AACCGCG	25	3.8856022E-5	45.0	1
TCGTTGT	20	7.0270087E-4	45.0	35
GCCTTGT	20	7.0270087E-4	45.0	45
GATACAC	20	7.0270087E-4	45.0	30
ACCGGTA	25	3.8856022E-5	45.0	29
ACACGTA	20	7.0270087E-4	45.0	25
TCGCACG	25	3.8856022E-5	45.0	1
CGAACCG	25	3.8856022E-5	45.0	28
TCCGCGA	40	6.7939254E-9	45.0	33
TCCGCAC	20	7.0270087E-4	45.0	8
>>END_MODULE