FastQCFastQC Report
Sat 18 Jun 2016
SRR3548804_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548804_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353300
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23540.6662892725728843No Hit
CTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGCT22910.6484574016416643No Hit
AATCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCT12920.3656948768751769No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCC12550.35522219077271444No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGT11370.32182281347296915No Hit
AAACTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCT10050.28446079818850833No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTT8270.23407868666855364No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5840.16529861307670535No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA4500.1273705066515709No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4240.12001132182281349No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCT3780.10699122558731956No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAG207.026647E-445.01
CTTCGTC253.885303E-545.037
AACCGTC207.026647E-445.029
GGTACTA207.026647E-445.08
GACAGAC207.026647E-445.09
GTCGCGA207.026647E-445.011
AGTCCGA253.885303E-545.041
GCCTTAT253.885303E-545.024
GAAGCGT207.026647E-445.012
GTTCTAC207.026647E-445.012
TCCGCGC253.885303E-545.021
CCCTCAT253.885303E-545.037
CCTAGGT253.885303E-545.024
TTAGCCG253.885303E-545.01
AACCCCG207.026647E-445.01
ACCGCGA207.026647E-445.044
ACACCCG351.2087366E-745.01
CGTGACC253.885303E-545.039
ATGTGCG253.885303E-545.01
CGCATAG253.885303E-545.01