##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548804_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	353300
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.40154542881404	31.0	31.0	34.0	30.0	34.0
2	31.57444664590999	31.0	31.0	34.0	30.0	34.0
3	31.782649306538353	31.0	31.0	34.0	30.0	34.0
4	35.62864138126238	37.0	35.0	37.0	33.0	37.0
5	35.22919048966884	37.0	35.0	37.0	33.0	37.0
6	35.24776110953864	37.0	35.0	37.0	32.0	37.0
7	35.20850551938862	37.0	35.0	37.0	32.0	37.0
8	35.52947919615058	37.0	35.0	37.0	33.0	37.0
9	36.361132182281345	39.0	37.0	39.0	32.0	39.0
10	36.08770733088027	38.0	35.0	39.0	31.0	39.0
11	36.325926974242854	38.0	35.0	39.0	32.0	39.0
12	35.823518256439286	37.0	35.0	39.0	31.0	39.0
13	35.39787715822247	37.0	35.0	39.0	30.0	39.0
14	36.4029408434758	38.0	35.0	40.0	31.0	41.0
15	36.52575997735635	38.0	35.0	40.0	31.0	41.0
16	36.635244834418344	38.0	35.0	40.0	31.0	41.0
17	36.62741579394282	38.0	35.0	40.0	32.0	41.0
18	36.481103877724316	38.0	35.0	40.0	31.0	41.0
19	36.47195301443532	38.0	35.0	40.0	31.0	41.0
20	36.39384658930088	38.0	35.0	40.0	31.0	41.0
21	36.389940560430226	38.0	35.0	40.0	31.0	41.0
22	36.35587602604019	38.0	35.0	40.0	31.0	41.0
23	36.28162468157373	38.0	35.0	40.0	31.0	41.0
24	35.965451457684686	38.0	34.0	40.0	30.0	41.0
25	36.02231531276536	38.0	34.0	40.0	30.0	41.0
26	35.90809227285593	38.0	34.0	40.0	30.0	41.0
27	35.841910557599775	38.0	34.0	40.0	30.0	41.0
28	35.74114350410416	38.0	34.0	40.0	30.0	41.0
29	35.56287291253892	37.0	34.0	40.0	29.0	41.0
30	35.55406170393433	37.0	34.0	40.0	29.0	41.0
31	35.235038211151995	37.0	34.0	40.0	27.0	41.0
32	35.205621285026886	37.0	34.0	40.0	28.0	41.0
33	35.13549391452024	37.0	34.0	40.0	27.0	41.0
34	34.968853665440136	37.0	34.0	40.0	27.0	41.0
35	34.94039626379847	37.0	34.0	40.0	27.0	41.0
36	34.68936880837815	37.0	33.0	40.0	25.0	41.0
37	34.494030568921595	36.0	33.0	40.0	25.0	41.0
38	34.40607698839513	36.0	33.0	40.0	24.0	41.0
39	34.37922728559298	36.0	33.0	40.0	24.0	41.0
40	34.2770591565242	36.0	33.0	40.0	24.0	41.0
41	34.258601754882534	36.0	33.0	40.0	24.0	41.0
42	34.209467874327764	36.0	33.0	40.0	24.0	41.0
43	34.10316161902066	36.0	33.0	40.0	24.0	41.0
44	33.951321822813476	35.0	33.0	40.0	23.0	41.0
45	33.678423436173226	35.0	33.0	39.0	23.0	41.0
46	33.697568638550806	35.0	33.0	39.0	23.0	41.0
47	33.59090008491367	35.0	33.0	39.0	23.0	41.0
48	33.59892442683272	35.0	33.0	39.0	23.0	41.0
49	33.48585904330597	35.0	33.0	39.0	23.0	40.0
50	33.24039909425417	35.0	32.0	39.0	22.0	40.0
51	31.275530710444382	34.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	5.0
11	4.0
12	5.0
13	14.0
14	27.0
15	43.0
16	85.0
17	190.0
18	392.0
19	718.0
20	1083.0
21	1629.0
22	2259.0
23	2874.0
24	3414.0
25	3827.0
26	4420.0
27	5206.0
28	6412.0
29	7977.0
30	10089.0
31	12922.0
32	16618.0
33	23135.0
34	39699.0
35	41389.0
36	32921.0
37	43701.0
38	53752.0
39	38485.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.22473818284744	34.84941975658081	19.457684687234643	25.468157373337107
2	23.60968015850552	33.89074440984999	20.486272289838663	22.01330314180583
3	20.73988112086046	32.69912255873196	21.69855646759128	24.862439852816305
4	21.084913671101045	34.256722332295496	18.33880554769318	26.319558448910275
5	17.595810925559014	36.79337673365412	17.30653835267478	28.30427398811209
6	20.383243702236058	38.20747240305689	19.712142654967447	21.697141239739597
7	79.56099632040758	9.175205208038493	6.173506934616473	5.090291536937447
8	81.9753750353807	7.330880271723747	4.906311916218511	5.787432776677045
9	74.88734786300594	11.834701386923294	8.495329748089443	4.782621001981319
10	44.9399943390886	28.747240305689214	11.23096518539485	15.081800169827343
11	37.58562128502689	26.28247947919615	20.209170676478912	15.922728559298047
12	35.75742994622134	24.639399943390885	23.340786866685537	16.262383243702235
13	24.79507500707614	36.591848287574294	20.380979337673367	18.232097367676197
14	18.02207755448627	37.68072459666006	26.75233512595528	17.544862722898387
15	16.120011321822815	29.365977922445513	35.21568072459666	19.298330031135013
16	16.926408151712426	25.52136994056043	35.30908576280781	22.243136144919333
17	17.92414378714973	25.75573167279932	27.74157939428248	28.57854514576847
18	22.935465609963206	25.892159637701674	25.957826210019814	25.214548542315313
19	24.146334559864137	30.333993772997452	24.237475233512594	21.282196433625813
20	27.68808378148882	29.444664590999153	22.451457684687234	20.415793942824795
21	21.89951882253043	31.26464760826493	22.909708463062554	23.926125106142088
22	21.547693178601754	29.332012454005095	23.443532408717804	25.67676195867535
23	22.11944523068214	30.794508915935463	21.41126521369941	25.674780639682986
24	19.22728559298047	29.710444381545432	27.67902632323804	23.383243702236058
25	20.42485140107557	28.021228417775262	27.114350410416076	24.439569770733087
26	21.159920747240303	32.76195867534673	23.23351259552788	22.844607981885083
27	19.18114916501557	30.184262666289275	25.215114633455986	25.419473535239174
28	20.810359467874328	30.121709595244834	28.448627228983863	20.61930370789697
29	18.837814888197	29.51684121143504	27.620718935748656	24.024624964619303
30	23.47410133031418	27.986979903764507	23.862156807245967	24.676761958675346
31	27.017831870931218	29.5887347863006	19.328332861590717	24.06510048117747
32	26.062553071044437	28.479196150580243	21.796490234927823	23.661760543447492
33	25.259835833569205	28.21539767902632	21.5864704217379	24.938296065666574
34	20.85451457684687	29.54259835833569	24.175488253608833	25.427398811208608
35	21.651853948485705	27.905462779507502	25.028870648174355	25.413812623832438
36	25.120294367393146	28.63911689782055	23.43305972261534	22.80752901217096
37	20.45485423153128	32.68525332578545	22.644777809227286	24.215114633455986
38	21.32550240588735	31.778658363996605	23.426549674497593	23.469289555618456
39	21.460515142938014	28.664590999150864	25.125106142088878	24.749787715822247
40	23.72261534106991	28.28332861590716	22.136994056043022	25.8570619869799
41	19.94565525049533	26.054061703934334	26.18709312199264	27.813189923577696
42	25.26662892725729	26.460232097367676	22.275969431078405	25.997169544296632
43	23.44636286442117	25.529861307670537	24.589017831870933	26.434757996037362
44	23.120577412963485	26.218228134729692	23.725445796773283	26.935748655533544
45	21.971978488536653	30.117180866119448	24.298046985564675	23.612793659779225
46	21.43787149731107	26.607698839513162	27.684121143504104	24.27030851967167
47	21.733654118313048	27.061137843192757	24.60005660911407	26.60515142938013
48	22.42060571752052	23.577979054627797	30.109255590150013	23.89215963770167
49	21.841211435041043	23.699405604302292	28.81715256156241	25.642230399094256
50	20.39654684404189	27.123124823096518	24.926125106142088	27.554203226719505
51	20.508349844324936	27.50014152278517	27.53382394565525	24.457684687234647
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2739.0
1	2182.5
2	1626.0
3	1145.0
4	664.0
5	645.0
6	626.0
7	661.0
8	696.0
9	777.0
10	858.0
11	918.5
12	979.0
13	1090.0
14	1201.0
15	1202.0
16	1203.0
17	1225.0
18	1247.0
19	1189.5
20	1132.0
21	1025.5
22	919.0
23	1097.0
24	1275.0
25	1170.0
26	1369.5
27	1674.0
28	2340.0
29	3006.0
30	3017.0
31	3028.0
32	4236.5
33	5445.0
34	5458.5
35	5472.0
36	6255.0
37	7038.0
38	8064.0
39	9090.0
40	11106.0
41	13122.0
42	18598.0
43	24074.0
44	28527.0
45	32980.0
46	34024.0
47	35068.0
48	34526.5
49	33985.0
50	32469.5
51	30954.0
52	28894.0
53	26834.0
54	23990.0
55	21146.0
56	19589.5
57	18033.0
58	16088.0
59	14143.0
60	13899.5
61	13656.0
62	12646.0
63	11636.0
64	9840.0
65	8044.0
66	6821.5
67	5599.0
68	4833.0
69	4067.0
70	3762.5
71	3458.0
72	2782.0
73	2106.0
74	1935.0
75	1244.5
76	725.0
77	593.0
78	461.0
79	345.0
80	229.0
81	174.0
82	119.0
83	85.0
84	51.0
85	38.5
86	26.0
87	21.5
88	17.0
89	12.5
90	8.0
91	7.0
92	6.0
93	5.0
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	353300.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.15808706001612
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.15446659299575	23.07992828107707
2	8.887314549795853	5.182741827453909
3	2.8977979248603165	2.534827325262335
4	1.3476156292852999	1.571755753685567
5	0.8140432465675838	1.1867971927017886
6	0.5043986894817173	0.882438054051957
7	0.3405933162045344	0.6951734696165988
8	0.3066337987866052	0.7152684000450638
9	0.2147435345719012	0.5635359608960768
>10	4.151042450593897	30.655482476927094
>50	1.260494603595763	24.912075422571043
>100	0.1128652061856745	4.890676672565297
>500	0.001997614268773	0.4109298389203617
>1k	0.005992842806319	2.7183693242258373
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2354	0.6662892725728843	No Hit
CTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGCT	2291	0.6484574016416643	No Hit
AATCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCT	1292	0.3656948768751769	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCC	1255	0.35522219077271444	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGT	1137	0.32182281347296915	No Hit
AAACTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCT	1005	0.28446079818850833	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTT	827	0.23407868666855364	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	584	0.16529861307670535	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	450	0.1273705066515709	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	424	0.12001132182281349	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCT	378	0.10699122558731956	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.830455703368242E-4	0.0	0.0	0.9448061137843192	0.0
2	2.830455703368242E-4	0.0	0.0	1.1709595244834419	0.0
3	2.830455703368242E-4	0.0	0.0	1.495895839230116	0.0
4	2.830455703368242E-4	0.0	0.0	3.4191904896688365	0.0
5	2.830455703368242E-4	0.0	0.0	3.70676478913105	0.0
6	2.830455703368242E-4	0.0	0.0	5.180583073874894	0.0
7	2.830455703368242E-4	0.0	0.0	6.303707896971412	0.0
8	2.830455703368242E-4	0.0	0.0	7.088593263515426	0.0
9	2.830455703368242E-4	0.0	0.0	8.416076988395131	0.0
10	2.830455703368242E-4	0.0	0.0	9.921030285876027	0.0
11	2.830455703368242E-4	0.0	0.0	11.661477497877158	0.0
12	2.830455703368242E-4	0.0	0.0	12.43758845174073	0.0
13	2.830455703368242E-4	0.0	0.0	12.755448627228985	0.0
14	2.830455703368242E-4	0.0	0.0	13.105859043305973	0.0
15	2.830455703368242E-4	0.0	0.0	13.348995188225304	0.0
16	2.830455703368242E-4	0.0	0.0	13.810359467874328	0.0
17	2.830455703368242E-4	0.0	0.0	14.362298330031136	0.0
18	2.830455703368242E-4	0.0	0.0	15.151146334559865	0.0
19	2.830455703368242E-4	0.0	0.0	15.472686102462497	0.0
20	2.830455703368242E-4	0.0	0.0	15.814322105859043	0.0
21	2.830455703368242E-4	0.0	0.0	16.14095669402774	0.0
22	2.830455703368242E-4	0.0	0.0	16.49448061137843	0.0
23	2.830455703368242E-4	0.0	0.0	16.828757429946222	0.0
24	2.830455703368242E-4	0.0	0.0	17.08095103311633	0.0
25	2.830455703368242E-4	0.0	0.0	17.275969431078405	0.0
26	2.830455703368242E-4	0.0	0.0	17.480611378431927	0.0
27	2.830455703368242E-4	0.0	0.0	17.694593829606568	0.0
28	2.830455703368242E-4	0.0	0.0	17.897820549108406	0.0
29	2.830455703368242E-4	0.0	0.0	18.114633455986414	0.0
30	2.830455703368242E-4	0.0	0.0	18.467591282196434	0.0
31	2.830455703368242E-4	0.0	0.0	18.699405604302292	0.0
32	2.830455703368242E-4	0.0	0.0	18.92244551372771	0.0
33	2.830455703368242E-4	0.0	0.0	19.132465326917632	0.0
34	2.830455703368242E-4	0.0	0.0	19.329465043872062	0.0
35	2.830455703368242E-4	0.0	0.0	19.56156241154826	0.0
36	2.830455703368242E-4	0.0	0.0	19.7701669968865	0.0
37	2.830455703368242E-4	0.0	0.0	19.98669685819417	0.0
38	2.830455703368242E-4	0.0	0.0	20.197282762524768	0.0
39	5.660911406736484E-4	0.0	0.0	20.42145485423153	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAG	20	7.026647E-4	45.0	1
CTTCGTC	25	3.885303E-5	45.0	37
AACCGTC	20	7.026647E-4	45.0	29
GGTACTA	20	7.026647E-4	45.0	8
GACAGAC	20	7.026647E-4	45.0	9
GTCGCGA	20	7.026647E-4	45.0	11
AGTCCGA	25	3.885303E-5	45.0	41
GCCTTAT	25	3.885303E-5	45.0	24
GAAGCGT	20	7.026647E-4	45.0	12
GTTCTAC	20	7.026647E-4	45.0	12
TCCGCGC	25	3.885303E-5	45.0	21
CCCTCAT	25	3.885303E-5	45.0	37
CCTAGGT	25	3.885303E-5	45.0	24
TTAGCCG	25	3.885303E-5	45.0	1
AACCCCG	20	7.026647E-4	45.0	1
ACCGCGA	20	7.026647E-4	45.0	44
ACACCCG	35	1.2087366E-7	45.0	1
CGTGACC	25	3.885303E-5	45.0	39
ATGTGCG	25	3.885303E-5	45.0	1
CGCATAG	25	3.885303E-5	45.0	1
>>END_MODULE