##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548802_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	553993
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.335556586455063	31.0	31.0	34.0	30.0	34.0
2	31.548346278743594	31.0	31.0	34.0	30.0	34.0
3	31.763152241995837	31.0	31.0	34.0	30.0	34.0
4	35.61375143729253	37.0	35.0	37.0	33.0	37.0
5	35.20900083575063	37.0	35.0	37.0	33.0	37.0
6	35.24290198612618	37.0	35.0	37.0	32.0	37.0
7	35.22470681037486	37.0	35.0	37.0	32.0	37.0
8	35.539690934723005	37.0	35.0	37.0	33.0	37.0
9	36.35942331401299	39.0	37.0	39.0	32.0	39.0
10	36.07863817773871	38.0	35.0	39.0	31.0	39.0
11	36.31773506163435	38.0	35.0	39.0	32.0	39.0
12	35.945918089217734	37.0	35.0	39.0	32.0	39.0
13	35.66181341641501	37.0	35.0	39.0	30.0	39.0
14	36.71176350603708	38.0	35.0	40.0	31.0	41.0
15	36.76638332975326	38.0	35.0	40.0	31.0	41.0
16	36.82987149657126	38.0	35.0	40.0	32.0	41.0
17	36.785802347683095	38.0	35.0	40.0	32.0	41.0
18	36.61349692144125	38.0	35.0	40.0	31.0	41.0
19	36.61160339571077	38.0	35.0	40.0	31.0	41.0
20	36.56873823315457	38.0	35.0	40.0	31.0	41.0
21	36.57793149010908	38.0	35.0	40.0	31.0	41.0
22	36.5686389539218	38.0	35.0	40.0	31.0	41.0
23	36.45654547981653	38.0	35.0	40.0	31.0	41.0
24	36.12479038543808	38.0	35.0	40.0	30.0	41.0
25	36.16647322258584	38.0	35.0	40.0	30.0	41.0
26	36.05962530212476	38.0	35.0	40.0	30.0	41.0
27	35.92468496894365	38.0	34.0	40.0	30.0	41.0
28	35.80563111808272	38.0	34.0	40.0	30.0	41.0
29	35.534689066468346	38.0	34.0	40.0	29.0	41.0
30	35.44547313774723	38.0	34.0	40.0	28.0	41.0
31	35.219109266723585	37.0	34.0	40.0	27.0	41.0
32	35.086694236208764	37.0	34.0	40.0	27.0	41.0
33	34.94707333847179	37.0	34.0	40.0	26.0	41.0
34	34.78056762450067	37.0	34.0	40.0	25.0	41.0
35	34.610235147375505	37.0	33.0	40.0	25.0	41.0
36	34.35737455166401	37.0	33.0	40.0	24.0	41.0
37	34.201298572364635	36.0	33.0	40.0	23.0	41.0
38	34.064869050691975	36.0	33.0	40.0	23.0	41.0
39	33.997254477944665	36.0	33.0	40.0	23.0	41.0
40	33.865427902518626	36.0	33.0	40.0	23.0	41.0
41	33.91413429411563	36.0	33.0	40.0	23.0	41.0
42	33.828293859308694	36.0	33.0	40.0	23.0	41.0
43	33.731270972737924	35.0	33.0	40.0	23.0	41.0
44	33.54898527598724	35.0	32.0	39.0	22.0	41.0
45	33.268339130638836	35.0	32.0	39.0	21.0	41.0
46	33.20162890144821	35.0	32.0	39.0	21.0	41.0
47	33.097044547494285	35.0	32.0	39.0	20.0	40.0
48	33.035870489338315	35.0	32.0	39.0	20.0	40.0
49	32.91103136682232	35.0	32.0	39.0	20.0	40.0
50	32.65764007848475	35.0	31.0	39.0	20.0	40.0
51	30.73907973566453	34.0	28.0	37.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	26.0
9	36.0
10	20.0
11	28.0
12	26.0
13	31.0
14	49.0
15	81.0
16	146.0
17	301.0
18	548.0
19	1138.0
20	1787.0
21	2712.0
22	3830.0
23	4976.0
24	5897.0
25	6837.0
26	7852.0
27	9267.0
28	11131.0
29	13106.0
30	16211.0
31	20415.0
32	26296.0
33	35648.0
34	57402.0
35	61774.0
36	54234.0
37	68446.0
38	84014.0
39	59723.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.281496336596312	32.45293713097458	20.033466126828316	26.232100405600793
2	24.259151288915206	32.46069896190024	18.857458487742626	24.42269126144193
3	20.48383282821263	31.52873772773302	21.578250988730904	26.409178455323445
4	21.56687900388633	32.5717111949068	18.599693497932286	27.261716303274593
5	17.635601893886747	36.65533680028448	18.29481599948014	27.414245306348633
6	20.78401712657019	37.872861209437666	19.68797439678841	21.655147267203738
7	81.1932641748181	8.537653002835775	5.655306113976169	4.6137767083699615
8	83.34166677196282	6.176431832171165	4.4182868736608585	6.063614522205154
9	77.19754581736592	10.020343217333071	8.18746807270128	4.594642892599726
10	45.59967364208573	27.47760350762555	11.608088910870714	15.314633939418007
11	36.26616220782573	26.7362584003769	19.956208832963593	17.041370558833776
12	33.8502472052896	23.449754780295056	24.865115624204638	17.83488239021071
13	26.018018278209293	31.701122577361083	22.395048312884818	19.885810831544802
14	19.481834608018513	32.644997319460714	28.133748982387864	19.73941909013291
15	17.310327025792745	27.044385037356065	33.599702523317085	22.045585413534106
16	19.777325706281488	23.305348623538563	33.79140169641133	23.12592397376862
17	19.74754193645046	24.205179487827465	27.46605101508503	28.581227560637046
18	22.373387389371345	24.85915887023843	27.804322437287116	24.963131303103108
19	24.613668403752396	30.268432994640726	23.720155308821592	21.397743292785286
20	28.3444014635564	28.507219405299345	21.061818470630495	22.08656066051376
21	22.83566759868807	30.185399454505742	23.210221067775226	23.768711879030963
22	22.699203780553184	27.22760034874087	23.55589330551108	26.517302565194868
23	22.503894453540028	29.121487094602276	21.56940611162957	26.805212340228124
24	20.979145945887403	29.147660710514394	26.75936338545794	23.113829958140265
25	23.01274564841072	26.742937185126888	24.70229768246169	25.5420194840007
26	23.72322393965267	30.277458379438006	21.34485453787322	24.654463143036104
27	20.33184534822642	28.34963618673882	24.33460350582047	26.98391495921428
28	22.940903585424365	27.802156344935767	26.540949073363745	22.715990996276126
29	23.025200679430966	28.58483771455596	25.32143907955516	23.068522526457915
30	25.274687586305244	26.625787690458182	24.06131485415881	24.038209869077768
31	29.854348340141485	28.87707967429191	18.413951078804246	22.85462090676236
32	28.94061838326477	25.39382266562935	21.287814105954407	24.377744845151472
33	27.908475377847736	27.55449978609838	20.619394107867787	23.917630728186097
34	23.507156227605762	28.753612410265113	22.75118999698552	24.988041365143605
35	24.947427133555838	25.943468599783753	24.393449014698742	24.715655251961667
36	27.073085761011424	29.07112544743345	22.652632794999214	21.203155996555914
37	23.13964255866049	30.60399680140363	21.229329612468028	25.027031027467856
38	23.895789296976677	30.803457805423534	22.119413061175862	23.181339836423927
39	21.898110625946536	28.592058022393786	24.40626506111088	25.103566290548795
40	25.188585415339183	27.455220553328292	21.502076741041854	25.854117290290674
41	21.887460671885744	23.56870935192322	25.915489906912182	28.628340069278853
42	25.629565716534326	25.06963084371102	21.820131301298034	27.480672138456626
43	23.127187527640242	24.32305101327995	24.737496683171088	27.81226477590872
44	22.699925811336964	24.09813842413171	25.348695741642945	27.853240022888375
45	24.095250300996582	26.536255873269155	24.99562268837332	24.372871137360942
46	23.24000483760625	25.61079291615598	28.04114853436776	23.108053711870006
47	23.72881967822698	25.90610350672301	24.297779935847565	26.067296879202445
48	23.845608157503793	23.056248003133614	29.13953786419684	23.95860597516575
49	24.079546131449312	21.612366943264625	27.606666510226663	26.701420415059395
50	21.563268849967418	24.70446377481304	26.300151806972288	27.43211556824725
51	21.99107208935853	25.05825885886645	26.189681097053573	26.76098795472145
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2703.0
1	2212.0
2	1721.0
3	1299.5
4	878.0
5	934.5
6	991.0
7	1087.5
8	1184.0
9	1322.0
10	1460.0
11	1528.0
12	1596.0
13	1742.5
14	1889.0
15	1819.0
16	1749.0
17	1703.0
18	1657.0
19	1555.5
20	1454.0
21	1369.0
22	1284.0
23	1412.0
24	1540.0
25	1856.5
26	2551.5
27	2930.0
28	2998.5
29	3067.0
30	3490.0
31	3913.0
32	4859.5
33	5806.0
34	6628.5
35	7451.0
36	8180.0
37	8909.0
38	10426.5
39	11944.0
40	14719.0
41	17494.0
42	23406.5
43	29319.0
44	38362.0
45	47405.0
46	49190.5
47	50976.0
48	50022.5
49	49069.0
50	49172.5
51	49276.0
52	46082.0
53	42888.0
54	38666.0
55	34444.0
56	31724.0
57	29004.0
58	27267.5
59	25531.0
60	24738.5
61	23946.0
62	22229.0
63	20512.0
64	19418.0
65	18324.0
66	15600.0
67	12876.0
68	12031.5
69	11187.0
70	9673.0
71	8159.0
72	7112.0
73	6065.0
74	5134.5
75	3531.0
76	2858.0
77	2312.5
78	1767.0
79	1496.0
80	1225.0
81	895.0
82	565.0
83	403.5
84	242.0
85	211.5
86	181.0
87	128.5
88	76.0
89	56.0
90	36.0
91	36.0
92	36.0
93	25.5
94	15.0
95	12.5
96	10.0
97	7.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	553993.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.38615879597964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.6624257274157	24.510212770221667
2	8.33420153369142	5.064887424808892
3	2.984025505161082	2.72019218553234
4	1.4028040058493283	1.7050330112549619
5	0.7746838378578366	1.1769833056913583
6	0.514866691583801	0.9386892629535633
7	0.3724412530875802	0.7921941340944998
8	0.25981735991617405	0.6315881245092049
9	0.22094724080058198	0.6042364150050032
>10	2.989493181787973	22.378176354280114
>50	1.2174875879403335	26.30601467252674
>100	0.2602986096665436	10.344334827561696
>500	0.002957938746210723	0.5667857800208885
>1k	0.0035495264954528674	2.260671731539072
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCT	2611	0.471305594114005	Illumina PCR Primer Index 6 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGCT	2406	0.43430151644515363	Illumina PCR Primer Index 6 (95% over 24bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCC	2242	0.4046982543100725	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2194	0.39603388490468294	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGT	2033	0.36697214585743865	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1090	0.19675338858072214	No Hit
AAACTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCT	821	0.14819681837135126	Illumina PCR Primer Index 6 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTT	722	0.13032655647273522	No Hit
AACTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTG	585	0.10559700212818574	Illumina PCR Primer Index 6 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8050769594561664E-4	0.0	0.0	0.7056045834514154	0.0
2	1.8050769594561664E-4	0.0	0.0	0.9160765569240045	0.0
3	1.8050769594561664E-4	0.0	0.0	1.2967672876733098	0.0
4	1.8050769594561664E-4	0.0	0.0	3.0516631076565948	0.0
5	1.8050769594561664E-4	0.0	0.0	3.3336161287236483	0.0
6	1.8050769594561664E-4	0.0	0.0	4.6987958331603465	0.0
7	1.8050769594561664E-4	0.0	0.0	5.8280519789961245	0.0
8	1.8050769594561664E-4	0.0	0.0	6.825356999095656	0.0
9	1.8050769594561664E-4	0.0	0.0	8.295953197964595	0.0
10	1.8050769594561664E-4	0.0	0.0	9.927923277008915	0.0
11	1.8050769594561664E-4	0.0	0.0	11.814228699640609	0.0
12	1.8050769594561664E-4	0.0	0.0	12.643661562510717	0.0
13	1.8050769594561664E-4	0.0	0.0	13.181574496428656	0.0
14	1.8050769594561664E-4	0.0	0.0	13.537896688225302	0.0
15	1.8050769594561664E-4	0.0	0.0	13.80938026292751	0.0
16	1.8050769594561664E-4	0.0	0.0	14.220576794291624	0.0
17	1.8050769594561664E-4	0.0	0.0	14.690257819142118	0.0
18	1.8050769594561664E-4	0.0	0.0	15.414635202971878	0.0
19	1.8050769594561664E-4	0.0	0.0	15.661750238721428	0.0
20	1.8050769594561664E-4	0.0	0.0	15.974028552707345	0.0
21	1.8050769594561664E-4	0.0	0.0	16.256884112254127	0.0
22	1.8050769594561664E-4	0.0	0.0	16.53540748709821	0.0
23	1.8050769594561664E-4	0.0	0.0	16.84263158559765	0.0
24	1.8050769594561664E-4	0.0	0.0	17.061948436171576	0.0
25	1.8050769594561664E-4	0.0	0.0	17.246066286036104	0.0
26	1.8050769594561664E-4	0.0	0.0	17.41375793556958	0.0
27	1.8050769594561664E-4	0.0	0.0	17.615746047332728	0.0
28	1.8050769594561664E-4	0.0	0.0	17.811416389737776	0.0
29	1.8050769594561664E-4	0.0	0.0	18.009252824494173	0.0
30	1.8050769594561664E-4	0.0	0.0	18.318823523040905	0.0
31	1.8050769594561664E-4	0.0	0.0	18.511966757702716	0.0
32	1.8050769594561664E-4	0.0	0.0	18.70366593079696	0.0
33	1.8050769594561664E-4	0.0	0.0	18.91955313514792	0.0
34	1.8050769594561664E-4	0.0	0.0	19.109988754370544	0.0
35	1.8050769594561664E-4	0.0	0.0	19.334359820430944	0.0
36	1.8050769594561664E-4	0.0	0.0	19.52317087039006	0.0
37	3.610153918912333E-4	0.0	0.0	19.712703951132955	0.0
38	3.610153918912333E-4	0.0	0.0	19.921551355342036	0.0
39	3.610153918912333E-4	0.0	0.0	20.12931571337544	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCCTTA	20	7.030117E-4	45.000004	35
CGAACCG	20	7.030117E-4	45.000004	1
CATATGT	20	7.030117E-4	45.000004	40
GTTTCGA	20	7.030117E-4	45.000004	26
TAGACTC	20	7.030117E-4	45.000004	43
TTAGCGT	20	7.030117E-4	45.000004	24
TCACACG	30	2.1634569E-6	45.000004	1
TCCGAAC	20	7.030117E-4	45.000004	29
ATCAGCG	20	7.030117E-4	45.000004	1
CAGCACG	40	6.8048394E-9	45.000004	1
ACACAAT	20	7.030117E-4	45.000004	35
TTCGTGC	20	7.030117E-4	45.000004	41
CTAAGCG	20	7.030117E-4	45.000004	1
GAATACG	20	7.030117E-4	45.000004	1
CGTTCCC	30	2.1634569E-6	45.000004	16
ATGTACG	20	7.030117E-4	45.000004	1
ACTAGGT	20	7.030117E-4	45.000004	31
TGCGAAG	20	7.030117E-4	45.000004	1
AATCTTC	30	2.1634569E-6	45.000004	39
AATCTCG	20	7.030117E-4	45.000004	18
>>END_MODULE