##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548801_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	569434
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.114770105051683	31.0	31.0	34.0	28.0	34.0
2	31.366657066490585	31.0	31.0	34.0	28.0	34.0
3	31.363564521963916	31.0	31.0	34.0	28.0	34.0
4	35.40135994689464	37.0	35.0	37.0	33.0	37.0
5	35.076345985662954	37.0	35.0	37.0	32.0	37.0
6	35.15223362145569	37.0	35.0	37.0	32.0	37.0
7	35.08795751570858	37.0	35.0	37.0	32.0	37.0
8	35.459017199534976	37.0	35.0	37.0	33.0	37.0
9	35.844519294597795	39.0	37.0	39.0	32.0	39.0
10	35.73751303926355	38.0	35.0	39.0	30.0	39.0
11	36.138944987478794	38.0	35.0	39.0	32.0	39.0
12	35.82883178735376	37.0	35.0	39.0	31.0	39.0
13	35.53220741999951	37.0	35.0	39.0	30.0	39.0
14	36.568705065029484	38.0	35.0	40.0	31.0	41.0
15	36.66353958492117	38.0	35.0	40.0	31.0	41.0
16	36.73762191930935	38.0	35.0	40.0	32.0	41.0
17	36.70145618280608	38.0	35.0	40.0	31.0	41.0
18	36.55545857816709	38.0	35.0	40.0	31.0	41.0
19	36.53942160109863	38.0	35.0	40.0	31.0	41.0
20	36.49040450693144	38.0	35.0	40.0	31.0	41.0
21	36.468637278420324	38.0	35.0	40.0	31.0	41.0
22	36.47491544235153	38.0	35.0	40.0	31.0	41.0
23	36.37459477305535	38.0	35.0	40.0	31.0	41.0
24	36.01178011850364	38.0	34.0	40.0	30.0	41.0
25	36.09685758138784	38.0	35.0	40.0	30.0	41.0
26	35.9905344605345	38.0	34.0	40.0	30.0	41.0
27	35.861297358429596	38.0	34.0	40.0	30.0	41.0
28	35.73970468921772	38.0	34.0	40.0	29.0	41.0
29	35.458611533557885	37.0	34.0	40.0	29.0	41.0
30	35.41523864047458	37.0	34.0	40.0	28.0	41.0
31	35.26948513787375	37.0	34.0	40.0	27.0	41.0
32	35.14012861894442	37.0	34.0	40.0	27.0	41.0
33	34.95590533758082	37.0	34.0	40.0	26.0	41.0
34	34.85815564226934	37.0	34.0	40.0	26.0	41.0
35	34.663063673753236	37.0	33.0	40.0	25.0	41.0
36	34.46705500549669	37.0	33.0	40.0	24.0	41.0
37	34.28269825827049	36.0	33.0	40.0	24.0	41.0
38	34.1898604579284	36.0	33.0	40.0	24.0	41.0
39	34.07097749695311	36.0	33.0	40.0	23.0	41.0
40	33.91979228497069	36.0	33.0	40.0	23.0	41.0
41	33.94790441034431	36.0	33.0	40.0	23.0	41.0
42	33.918801827779866	36.0	33.0	40.0	23.0	41.0
43	33.839702230636036	35.0	33.0	40.0	23.0	41.0
44	33.76523179156847	35.0	33.0	39.0	23.0	41.0
45	33.536404570152115	35.0	32.0	39.0	23.0	41.0
46	33.35280822711675	35.0	32.0	39.0	22.0	41.0
47	33.19802646136339	35.0	32.0	39.0	22.0	40.0
48	33.08576586575442	35.0	32.0	39.0	21.0	40.0
49	32.94708956613058	35.0	32.0	39.0	20.0	40.0
50	32.81710610887302	35.0	31.0	39.0	20.0	40.0
51	31.02586427926678	34.0	28.0	37.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	16.0
9	19.0
10	25.0
11	20.0
12	23.0
13	25.0
14	45.0
15	101.0
16	157.0
17	299.0
18	606.0
19	1112.0
20	1825.0
21	2797.0
22	3950.0
23	4879.0
24	5866.0
25	6811.0
26	8028.0
27	9321.0
28	11269.0
29	13947.0
30	16913.0
31	21334.0
32	28217.0
33	37970.0
34	60497.0
35	63056.0
36	57259.0
37	72111.0
38	84263.0
39	56668.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.45850089738231	32.04497799569397	20.209190178317417	26.287330928606302
2	24.51153250420593	32.03198263538882	18.973401658488957	24.48308320191629
3	20.912871377543315	31.357804416315147	21.273404819522543	26.45591938661899
4	21.595479019517626	32.521064776602735	18.46394841193185	27.419507791947794
5	17.791526322629135	36.46849327577911	18.2572519378892	27.482728463702554
6	20.636632164570436	38.05796633148003	19.610701152372357	21.694700351577183
7	81.68321526287507	8.461033236512046	5.357249479307523	4.498502021305367
8	83.94950073230612	6.110453538074649	4.206808866347988	5.733236863271248
9	76.99224141867187	10.961235191435707	7.639866955608551	4.406656434283867
10	45.95124281303891	27.523821900343147	11.521791814327912	15.003143472290029
11	36.58123680707509	26.877741757604923	19.5267581493202	17.01426328599978
12	34.26437480024024	23.616959998876077	24.515571602679152	17.603093598204534
13	26.026545657617916	31.912741423940265	22.333931588208642	19.72678133023318
14	19.781572579087303	32.85367575522361	27.648682727058798	19.716068938630286
15	17.396397124161886	27.0317543385186	33.78319524299568	21.788653294323836
16	20.11119813709754	23.188288721783383	33.78986853612535	22.91064460499373
17	20.062026503510502	23.82277840803324	27.42337127744392	28.69182381101234
18	22.65583017522663	24.89998840954351	27.77916316904154	24.665018246188318
19	25.000614645419837	30.09181046442608	23.549700228648096	21.35787466150599
20	28.70798020490522	28.336207532391818	20.955545330977777	22.000266931725186
21	23.088891776746735	29.995047714045874	23.21515750727916	23.700903001928232
22	22.93874268132918	27.13905386752459	23.459961997351755	26.462241453794473
23	22.434733437062064	29.055167060625113	21.81359033707155	26.696509165241274
24	21.19859369127941	28.87990530948275	26.995402452259615	22.926098546978228
25	23.108911656135742	26.820667540048536	24.68942845000474	25.380992353810978
26	23.615203869105112	30.300965520148075	21.371221247765323	24.712609362981485
27	20.396042385948153	28.724136598798104	24.280425826346864	26.599395188906875
28	22.76190041339295	27.940200269039078	26.743924669057346	22.55397464851063
29	22.87762936529958	28.617012682769204	25.541678227854327	22.96367972407689
30	25.190803499615406	26.63873249577651	24.448311832451168	23.722152172156914
31	29.945349241527552	28.720097500324883	18.659581268417412	22.674971989730153
32	29.089060365204748	25.548175908006897	21.391416740131426	23.971346986656926
33	27.802519694995382	27.540856359121513	20.79679119968249	23.859832746200613
34	23.52300705612942	28.70657530108845	22.935932873695634	24.834484769086494
35	24.917198481298975	26.049550957617562	24.624451648479013	24.408798912604446
36	27.22124074080578	29.05428899573963	22.526754637060662	21.19771562639393
37	23.085906356136093	30.736485703347533	21.460432640130374	24.717175300385996
38	23.867384104215766	30.83254600181935	22.06278515157156	23.237284742393324
39	22.025028361495803	28.73994176673679	24.276737953827837	24.95829191793957
40	25.281771021751425	27.414941854543283	21.421446559214942	25.881840564490354
41	21.994471703481	23.70511771337855	25.85216197136104	28.448248611779412
42	25.501989695030503	25.147602707249657	21.913162895085296	27.437244702634544
43	23.192854659187894	24.590382730922283	24.514869150770764	27.701893459119052
44	22.711148263012042	24.467453646954695	25.360270022513582	27.46112806751968
45	23.91304347826087	26.840863032414642	24.88172465992547	24.364368829399016
46	23.315783743155485	25.972983699603464	27.700839781256477	23.010392775984574
47	23.837705511086448	26.247642395782478	24.232307870622407	25.682344222508668
48	23.920419223298925	23.32456439201031	28.85286091100988	23.90215547368088
49	24.09708587825806	21.974978663023283	27.637794722478816	26.29014073623985
50	21.80287794546866	25.159193163738024	26.295584738529836	26.742344152263474
51	22.03890178668643	25.518146088923388	25.804922080522065	26.63803004386812
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2751.0
1	2314.5
2	1878.0
3	1397.5
4	917.0
5	926.0
6	935.0
7	1071.5
8	1208.0
9	1288.0
10	1368.0
11	1522.0
12	1676.0
13	1735.5
14	1795.0
15	1777.5
16	1760.0
17	1744.0
18	1728.0
19	1581.5
20	1435.0
21	1399.5
22	1364.0
23	1460.0
24	1556.0
25	1926.0
26	2681.0
27	3066.0
28	3198.0
29	3330.0
30	3768.5
31	4207.0
32	5245.5
33	6284.0
34	6981.5
35	7679.0
36	8662.0
37	9645.0
38	11248.0
39	12851.0
40	15643.0
41	18435.0
42	24567.0
43	30699.0
44	39619.0
45	48539.0
46	50196.0
47	51853.0
48	50526.0
49	49199.0
50	49641.0
51	50083.0
52	46916.5
53	43750.0
54	39351.0
55	34952.0
56	32307.5
57	29663.0
58	27969.5
59	26276.0
60	25614.0
61	24952.0
62	22992.0
63	21032.0
64	19989.0
65	18946.0
66	16117.5
67	13289.0
68	12442.0
69	11595.0
70	10084.5
71	8574.0
72	7425.0
73	6276.0
74	5349.5
75	3690.5
76	2958.0
77	2402.0
78	1846.0
79	1518.5
80	1191.0
81	884.5
82	578.0
83	408.5
84	239.0
85	200.5
86	162.0
87	123.5
88	85.0
89	62.0
90	39.0
91	43.0
92	47.0
93	31.0
94	15.0
95	9.5
96	4.0
97	4.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	569434.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.015776644780296
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.20583126850207	24.074403169642938
2	8.488291198331346	5.095653054099364
3	3.0720571522973574	2.7663054397007185
4	1.5217280608679737	1.8270339835643097
5	0.8566766100060113	1.2856906891373996
6	0.5544637865189734	0.9985596704263593
7	0.39002547417419536	0.8194842263011022
8	0.27685113405194745	0.6647921442845907
9	0.19584677913702986	0.5290643861259035
>10	2.9512572486544224	22.138087834646655
>50	1.1810028669350188	25.451509876891702
>100	0.2989211632371579	11.616109580343036
>500	0.0035236286432670867	0.6164304713718108
>1k	0.0035236286432670867	2.1168754734641344
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCT	2449	0.4300761809094645	Illumina PCR Primer Index 6 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2241	0.3935486816733809	No Hit
CTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGCT	2177	0.3823094511392014	Illumina PCR Primer Index 6 (95% over 24bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCC	2125	0.3731775763301805	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGT	1934	0.3396354977047384	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1083	0.19018885419556963	No Hit
AAACTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCT	766	0.13451954045596154	Illumina PCR Primer Index 6 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTT	659	0.1157289519066301	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.5122595419311107E-4	0.0	0.0	0.6065672228915028	0.0
2	3.5122595419311107E-4	0.0	0.0	0.8027269183083553	0.0
3	3.5122595419311107E-4	0.0	0.0	1.1476308053259903	0.0
4	3.5122595419311107E-4	0.0	0.0	2.6953079724779343	0.0
5	3.5122595419311107E-4	0.0	0.0	2.966454409115016	0.0
6	3.5122595419311107E-4	0.0	0.0	4.1953940228367115	0.0
7	3.5122595419311107E-4	0.0	0.0	5.2287007800728444	0.0
8	3.5122595419311107E-4	0.0	0.0	6.10378024494498	0.0
9	3.5122595419311107E-4	0.0	0.0	7.430185060955265	0.0
10	3.5122595419311107E-4	0.0	0.0	8.940456663985643	0.0
11	3.5122595419311107E-4	0.0	0.0	10.678849524264445	0.0
12	3.5122595419311107E-4	0.0	0.0	11.437497585321564	0.0
13	3.5122595419311107E-4	0.0	0.0	11.942209281497066	0.0
14	3.5122595419311107E-4	0.0	0.0	12.280264262407934	0.0
15	3.5122595419311107E-4	0.0	0.0	12.523839461640858	0.0
16	3.5122595419311107E-4	0.0	0.0	12.908256268505218	0.0
17	3.5122595419311107E-4	0.0	0.0	13.345708194452737	0.0
18	3.5122595419311107E-4	0.0	0.0	14.007944731083848	0.0
19	3.5122595419311107E-4	0.0	0.0	14.228865856271314	0.0
20	3.5122595419311107E-4	0.0	0.0	14.518627268480632	0.0
21	3.5122595419311107E-4	0.0	0.0	14.792759125728354	0.0
22	3.5122595419311107E-4	0.0	0.0	15.06829588679285	0.0
23	3.5122595419311107E-4	0.0	0.0	15.362272010452484	0.0
24	3.5122595419311107E-4	0.0	0.0	15.561241513502882	0.0
25	3.5122595419311107E-4	0.0	0.0	15.744757074568783	0.0
26	3.5122595419311107E-4	0.0	0.0	15.911940628764704	0.0
27	3.5122595419311107E-4	0.0	0.0	16.106168581433494	0.0
28	3.5122595419311107E-4	0.0	0.0	16.29126465929326	0.0
29	3.5122595419311107E-4	0.0	0.0	16.483560869213992	0.0
30	3.5122595419311107E-4	0.0	0.0	16.78807377149942	0.0
31	3.5122595419311107E-4	0.0	0.0	16.973521075313382	0.0
32	3.5122595419311107E-4	0.0	0.0	17.163885542486046	0.0
33	3.5122595419311107E-4	0.0	0.0	17.376201631795784	0.0
34	3.5122595419311107E-4	0.0	0.0	17.567795389808126	0.0
35	3.5122595419311107E-4	0.0	0.0	17.787662837133013	0.0
36	3.5122595419311107E-4	0.0	0.0	17.9616952974357	0.0
37	3.5122595419311107E-4	0.0	0.0	18.154518346287716	0.0
38	3.5122595419311107E-4	0.0	0.0	18.359458690559396	0.0
39	3.5122595419311107E-4	0.0	0.0	18.569491811166877	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	25	3.8883176E-5	45.0	1
CCTTAGG	25	3.8883176E-5	45.0	28
TCCGCAA	20	7.0302846E-4	45.0	32
GTATTCG	25	3.8883176E-5	45.0	25
ACGTTAG	20	7.0302846E-4	45.0	1
TTAGCCG	40	6.8048394E-9	45.0	1
TCCGAAC	20	7.0302846E-4	45.0	29
TCTTCGA	25	3.8883176E-5	45.0	32
TTTCGAA	20	7.0302846E-4	45.0	20
CGTGAGC	20	7.0302846E-4	45.0	13
GGCAATC	40	6.8048394E-9	45.0	8
ACTCTAC	20	7.0302846E-4	45.0	44
ATAGCGC	25	3.8883176E-5	45.0	14
ATAGCCG	25	3.8883176E-5	45.0	1
AAGACCG	20	7.0302846E-4	45.0	31
TAAACCA	20	7.0302846E-4	45.0	30
CCGTGAC	20	7.0302846E-4	45.0	29
CGACCGA	20	7.0302846E-4	45.0	13
TTCGTGC	20	7.0302846E-4	45.0	39
TTCGTGA	25	3.8883176E-5	45.0	28
>>END_MODULE