FastQCFastQC Report
Sat 18 Jun 2016
SRR3548800_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548800_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291990
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCC28850.9880475358745162No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGT22930.7853008664680297No Hit
AATCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCT22810.7811911366827631No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21790.7462584335079968No Hit
CTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGCT20230.6928319462995308No Hit
AAACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCT7160.2452138771875749No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5500.18836261515805336No Hit
AATGACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTT5270.18048563306962567No Hit
AAAAACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTT5170.17706085824857015No Hit
AATGATCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCT4140.14178567759169836No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3970.135963560395904No Hit
AACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTG3620.12397684852220967No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTAT3480.11918216377273193No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCT3030.10377067707798213No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCATAT302.1599208E-645.00000431
GCTAATG302.1599208E-645.0000041
TGCGTAG302.1599208E-645.0000041
TGATCGG253.8836348E-545.02
CTATGTG207.024633E-445.013
TAATAGT207.024633E-445.042
GCCCACA207.024633E-445.027
AAATCCG253.8836348E-545.01
CAAGTTT207.024633E-445.011
TGCCAGT207.024633E-445.024
CAAAACG207.024633E-445.01
CGCGGAT207.024633E-445.023
GCGGTCA207.024633E-445.040
CGTGCCC253.8836348E-545.024
GCAACCG207.024633E-445.01
TAGGTGC207.024633E-445.021
TAAACGG453.8198777E-1045.02
GGATCAC351.2077726E-745.08
TGACGTT207.024633E-445.025
CACGACC351.2077726E-745.027