##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548800_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	291990
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.453460734956675	31.0	31.0	34.0	30.0	34.0
2	31.627703003527518	31.0	31.0	34.0	30.0	34.0
3	31.812873728552347	31.0	31.0	34.0	30.0	34.0
4	35.629247576971814	37.0	35.0	37.0	33.0	37.0
5	35.2266755710812	37.0	35.0	37.0	33.0	37.0
6	35.228542073358675	37.0	35.0	37.0	32.0	37.0
7	35.166115277920476	37.0	35.0	37.0	32.0	37.0
8	35.503277509503754	37.0	35.0	37.0	33.0	37.0
9	36.398065002226105	39.0	37.0	39.0	32.0	39.0
10	36.134045686496115	38.0	35.0	39.0	31.0	39.0
11	36.330525017980065	38.0	35.0	39.0	32.0	39.0
12	35.807147505051546	37.0	35.0	39.0	31.0	39.0
13	35.47595808075619	37.0	35.0	39.0	30.0	39.0
14	36.458762286379674	38.0	35.0	40.0	31.0	41.0
15	36.57648549607863	38.0	35.0	40.0	31.0	41.0
16	36.591705195383405	38.0	35.0	40.0	31.0	41.0
17	36.56343025446077	38.0	35.0	40.0	31.0	41.0
18	36.34272406589267	38.0	35.0	40.0	31.0	41.0
19	36.326500907565325	38.0	35.0	40.0	31.0	41.0
20	36.320685639919176	38.0	34.0	40.0	31.0	41.0
21	36.364574129251004	38.0	35.0	40.0	31.0	41.0
22	36.35838898592417	38.0	35.0	40.0	31.0	41.0
23	36.28582828179047	38.0	35.0	40.0	31.0	41.0
24	35.938470495564914	38.0	34.0	40.0	30.0	41.0
25	36.054539539025306	38.0	34.0	40.0	30.0	41.0
26	35.918863659714376	38.0	34.0	40.0	30.0	41.0
27	35.849700332203156	38.0	34.0	40.0	30.0	41.0
28	35.82797356073838	38.0	34.0	40.0	30.0	41.0
29	35.513794992979214	37.0	34.0	40.0	29.0	41.0
30	35.5457549916093	37.0	34.0	40.0	29.0	41.0
31	35.31671975067639	37.0	34.0	40.0	29.0	41.0
32	35.231124353573755	37.0	34.0	40.0	28.0	41.0
33	35.24626185828282	37.0	34.0	40.0	28.0	41.0
34	35.074571046953665	37.0	34.0	40.0	27.0	41.0
35	34.932333299085585	37.0	34.0	40.0	27.0	41.0
36	34.64617623891229	37.0	33.0	40.0	25.0	41.0
37	34.54212130552416	36.0	33.0	40.0	25.0	41.0
38	34.444429603753555	36.0	33.0	40.0	25.0	41.0
39	34.28582828179047	36.0	33.0	40.0	24.0	41.0
40	34.139655467653	36.0	33.0	40.0	24.0	41.0
41	34.16394739545875	36.0	33.0	40.0	24.0	41.0
42	34.01694236103976	36.0	33.0	40.0	23.0	41.0
43	33.8110928456454	35.0	33.0	40.0	23.0	41.0
44	33.61462378848591	35.0	33.0	39.0	23.0	41.0
45	33.287064625500875	35.0	32.0	39.0	21.0	41.0
46	33.14967635877941	35.0	32.0	39.0	21.0	40.0
47	33.04092605911161	35.0	32.0	39.0	20.0	40.0
48	33.019853419637656	35.0	32.0	39.0	20.0	40.0
49	32.90756190280489	35.0	32.0	39.0	20.0	40.0
50	32.59416418370492	35.0	31.0	39.0	18.0	40.0
51	30.73406623514504	34.0	28.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	0.0
10	4.0
11	4.0
12	5.0
13	7.0
14	19.0
15	31.0
16	74.0
17	145.0
18	251.0
19	502.0
20	881.0
21	1359.0
22	1883.0
23	2290.0
24	2806.0
25	3382.0
26	4023.0
27	4710.0
28	5671.0
29	6992.0
30	8628.0
31	11212.0
32	14221.0
33	19618.0
34	32970.0
35	34274.0
36	27474.0
37	35050.0
38	43239.0
39	30261.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.903421350046237	36.284804274118976	19.59827391349019	24.2135004623446
2	21.839789033871025	36.06048152333984	20.051714099797938	22.0480153429912
3	19.524641254837494	32.09561971300387	23.01106202267201	25.36867700948663
4	21.350731189424295	32.23603548066715	19.10818863659714	27.305044693311416
5	16.51563409705812	38.25302236377958	18.747217370457893	26.484126168704407
6	20.774341587040652	36.84646734477208	21.216822493921025	21.162368574266242
7	77.0879139696565	10.785643343950134	6.7885886502962425	5.337854036097127
8	80.1479502722696	6.709476351929861	4.949142093907326	8.193431281893215
9	74.80153429911984	10.914757354703928	9.36025206342683	4.923456282749409
10	45.03544641939792	29.029076338230762	11.075721771293537	14.859755471077776
11	36.43001472653173	24.025480324668653	22.627144765231684	16.91736018356793
12	33.90938045823487	22.987431076406725	26.961882256241655	16.141306209116753
13	25.087845474160076	34.711120243843965	21.96650570225008	18.23452857974588
14	16.956402616527964	34.74331312716189	29.813007294770365	18.487276961539777
15	15.142641871296963	27.991027089968835	35.02071988766738	21.84561115106682
16	17.163259015719717	25.30155142299394	34.825165245385115	22.71002431590123
17	17.895475872461386	25.51217507448885	27.43792595636837	29.15442309668139
18	21.42744614541594	26.364601527449572	27.28620843179561	24.92174389533888
19	23.56861536353985	32.03363128874276	25.38066372136032	19.017089626357066
20	26.348162608308506	30.327750950375016	22.640843864515908	20.683242576800577
21	20.97777321141135	31.457241686359122	23.322374053905957	24.242611048323575
22	20.436316312202475	29.46162539813007	24.632692900441796	25.469365389225658
23	21.973697729374294	30.163704236446453	21.012705914586117	26.84989211959314
24	19.558546525565944	31.50587348881811	26.539949998287614	22.395629987328334
25	19.393814856673174	26.655364909757186	26.532757971163395	27.418062262406245
26	20.28014658036234	33.65354977910203	22.620295215589575	23.44600842494606
27	18.156786191307923	29.69279769855132	26.97215658070482	25.178259529435937
28	20.23630946265283	27.844104250145552	30.39864378917086	21.520942498030752
29	18.99037638275283	32.06993390184596	27.36395082023357	21.575738895167643
30	25.013870338025274	27.295112846330355	27.179012979896573	20.512003835747798
31	29.422925442652147	28.90509948970855	20.10342819959588	21.568546868043427
32	27.978697900613035	29.00750025685811	20.784273434021713	22.22952840850714
33	27.433473749100994	25.99712318915031	25.16798520497277	21.401417856775918
34	21.28052330559266	28.602691873009352	23.58710914757355	26.52967567382445
35	24.814548443439843	26.87489297578684	28.23692592212062	20.07363265865269
36	26.62625432377821	28.107126956402617	26.450220897975957	18.816397821843214
37	25.341278810918183	31.47368060550019	21.47128326312545	21.713757320456182
38	26.383437788965374	28.56125209767458	24.238501318538304	20.81680879482174
39	22.528511250385286	28.03760402753519	25.500188362615155	23.933696359464367
40	26.05431692866194	25.481009623617247	24.000821945957053	24.46385150176376
41	21.14181992533991	21.184972088085207	29.042432960032876	28.630775026542004
42	27.274221719921915	24.373437446487895	22.214459399294498	26.137881434295696
43	24.168635912188773	24.468988663995344	25.05496763587794	26.30740778793794
44	22.19048597554711	24.67310524333025	25.500188362615155	27.636220418507484
45	25.2806602965855	27.64580978800644	23.894996403986436	23.178533511421623
46	22.209322237062914	25.900887016678652	29.469502380218497	22.420288366039934
47	22.95421076064249	25.891297647179695	25.407376964964552	25.747114627213264
48	23.5778622555567	20.657214288160553	31.992876468372206	23.772046987910546
49	23.268262611733277	20.44111099695195	30.106510496934824	26.184115894379943
50	20.420562348025616	24.236103976163566	26.917702661050036	28.42563101476078
51	20.2407616699202	23.992260008904413	29.91746292681256	25.84951539436282
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2492.0
1	2000.5
2	1509.0
3	1033.5
4	558.0
5	544.5
6	531.0
7	578.0
8	625.0
9	689.0
10	753.0
11	841.0
12	929.0
13	970.5
14	1012.0
15	989.5
16	967.0
17	951.5
18	936.0
19	959.0
20	982.0
21	932.5
22	883.0
23	876.5
24	870.0
25	1235.5
26	1688.0
27	1775.0
28	2148.0
29	2521.0
30	2454.5
31	2388.0
32	2997.5
33	3607.0
34	4432.5
35	5258.0
36	5225.0
37	5192.0
38	5677.5
39	6163.0
40	8361.5
41	10560.0
42	14116.0
43	17672.0
44	23790.0
45	29908.0
46	30883.5
47	31859.0
48	30193.5
49	28528.0
50	26678.0
51	24828.0
52	22905.0
53	20982.0
54	18857.5
55	16733.0
56	15133.0
57	13533.0
58	12538.0
59	11543.0
60	11258.5
61	10974.0
62	10685.5
63	10397.0
64	8896.5
65	7396.0
66	6281.0
67	5166.0
68	4222.0
69	3278.0
70	2889.0
71	2500.0
72	1995.0
73	1490.0
74	1403.5
75	1043.0
76	769.0
77	623.5
78	478.0
79	368.0
80	258.0
81	191.0
82	124.0
83	80.0
84	36.0
85	28.5
86	21.0
87	18.5
88	16.0
89	25.0
90	34.0
91	31.0
92	28.0
93	18.5
94	9.0
95	5.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	291990.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.75081338402
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.90391581852683	27.30572964827563
2	7.762635846127306	5.239905476214939
3	2.5337649291215536	2.565498818452687
4	1.21158002617987	1.635672454536114
5	0.7123359952916822	1.202095962190486
6	0.5307004637287035	1.0746943388472208
7	0.40284528508660666	0.9517449227713278
8	0.3612416158459244	0.9753758690366109
9	0.2891962374047428	0.8784547416007397
>10	4.663669849516484	38.9263330935991
>50	0.5783924748094856	11.966848179732184
>100	0.04058894560066566	2.49289359224631
>500	0.0040588945600665656	0.7911229836638242
>1k	0.005073618200083207	3.993629918832837
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCC	2885	0.9880475358745162	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGT	2293	0.7853008664680297	No Hit
AATCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCT	2281	0.7811911366827631	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2179	0.7462584335079968	No Hit
CTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGCT	2023	0.6928319462995308	No Hit
AAACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCT	716	0.2452138771875749	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	550	0.18836261515805336	No Hit
AATGACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTT	527	0.18048563306962567	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTT	517	0.17706085824857015	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCT	414	0.14178567759169836	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	397	0.135963560395904	No Hit
AACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTG	362	0.12397684852220967	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTAT	348	0.11918216377273193	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCT	303	0.10377067707798213	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0565430322956266	0.0
2	0.0	0.0	0.0	1.3431966848179733	0.0
3	0.0	0.0	0.0	1.8120483578204734	0.0
4	0.0	0.0	0.0	4.486455015582726	0.0
5	0.0	0.0	0.0	4.855303263810405	0.0
6	0.0	0.0	0.0	6.485153601150724	0.0
7	0.0	0.0	0.0	7.803349429774992	0.0
8	0.0	0.0	0.0	8.656460837699921	0.0
9	0.0	0.0	0.0	10.763382307613275	0.0
10	0.0	0.0	0.0	12.518236925922121	0.0
11	0.0	0.0	0.0	15.344018630775027	0.0
12	0.0	0.0	0.0	16.270762697352648	0.0
13	0.0	0.0	0.0	16.694749820199323	0.0
14	0.0	0.0	0.0	17.22148018767766	0.0
15	0.0	0.0	0.0	17.556080687694784	0.0
16	0.0	0.0	0.0	18.131100380150006	0.0
17	0.0	0.0	0.0	18.770163361758964	0.0
18	0.0	0.0	0.0	19.74142950101031	0.0
19	0.0	0.0	0.0	20.11472995650536	0.0
20	0.0	0.0	0.0	20.490085276893044	0.0
21	0.0	0.0	0.0	20.899688345491285	0.0
22	0.0	0.0	0.0	21.279153395664235	0.0
23	0.0	0.0	0.0	21.704852905921435	0.0
24	0.0	0.0	0.0	21.976095071749032	0.0
25	0.0	0.0	0.0	22.22336381382924	0.0
26	0.0	0.0	0.0	22.443234357341005	0.0
27	0.0	0.0	0.0	22.700092468920168	0.0
28	0.0	0.0	0.0	22.940511661358265	0.0
29	0.0	0.0	0.0	23.20421932257954	0.0
30	0.0	0.0	0.0	23.576492345628274	0.0
31	0.0	0.0	0.0	23.821363745333745	0.0
32	0.0	0.0	0.0	24.060755505325524	0.0
33	0.0	0.0	0.0	24.307339292441522	0.0
34	0.0	0.0	0.0	24.57378677351964	0.0
35	0.0	0.0	3.4247748210555155E-4	24.85393335388198	0.0
36	0.0	0.0	3.4247748210555155E-4	25.07277646494743	0.0
37	0.0	0.0	3.4247748210555155E-4	25.29983903558341	0.0
38	0.0	0.0	3.4247748210555155E-4	25.55703962464468	0.0
39	0.0	0.0	3.4247748210555155E-4	25.821432240830166	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCATAT	30	2.1599208E-6	45.000004	31
GCTAATG	30	2.1599208E-6	45.000004	1
TGCGTAG	30	2.1599208E-6	45.000004	1
TGATCGG	25	3.8836348E-5	45.0	2
CTATGTG	20	7.024633E-4	45.0	13
TAATAGT	20	7.024633E-4	45.0	42
GCCCACA	20	7.024633E-4	45.0	27
AAATCCG	25	3.8836348E-5	45.0	1
CAAGTTT	20	7.024633E-4	45.0	11
TGCCAGT	20	7.024633E-4	45.0	24
CAAAACG	20	7.024633E-4	45.0	1
CGCGGAT	20	7.024633E-4	45.0	23
GCGGTCA	20	7.024633E-4	45.0	40
CGTGCCC	25	3.8836348E-5	45.0	24
GCAACCG	20	7.024633E-4	45.0	1
TAGGTGC	20	7.024633E-4	45.0	21
TAAACGG	45	3.8198777E-10	45.0	2
GGATCAC	35	1.2077726E-7	45.0	8
TGACGTT	20	7.024633E-4	45.0	25
CACGACC	35	1.2077726E-7	45.0	27
>>END_MODULE