FastQCFastQC Report
Sat 18 Jun 2016
SRR3548795_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548795_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461966
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCTGCT27080.5861903256949645TruSeq Adapter, Index 22 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27030.5851079949606681No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCC24900.5390007056796388No Hit
AATCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCT23150.5011191299792626No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGT18790.40673988994861093No Hit
AAACTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTTCT10650.23053644640514667No Hit
AAAAACTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTT9500.20564283951632806No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6000.1298796881155756No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCT5320.1151599901291437No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5180.1121294640731136No Hit
AATGACTGTCTCTTATACACATCTGACGCTTAGCTCCTCGTATGCCGTCTT5080.10996480260452067No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTCG207.0289E-445.00000431
CAGCGAT207.0289E-445.00000421
AATCCCG207.0289E-445.0000041
GGTACCG207.0289E-445.0000041
TCCGCCA207.0289E-445.00000435
GGTCGGT207.0289E-445.00000442
CTCCGCG207.0289E-445.0000041
GGTCGAT207.0289E-445.00000435
ACGCACG207.0289E-445.0000041
TAGCGAA207.0289E-445.00000438
ATAGCCC207.0289E-445.00000435
CCAGCGT207.0289E-445.00000436
CATCTAC207.0289E-445.00000414
TAACGCG207.0289E-445.0000041
ACTTACG207.0289E-445.0000041
GCCGCTA207.0289E-445.00000416
GCCGATT207.0289E-445.00000417
AGGCCGC207.0289E-445.00000414
CTACGTA207.0289E-445.00000433
ATATACG207.0289E-445.0000041