FastQCFastQC Report
Sat 18 Jun 2016
SRR3548794_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548794_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479890
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGCT39100.8147700514701286No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36050.7512138198337119No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCC27030.5632540790597845No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGT25850.5386651107545479No Hit
AATCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCT25430.5299131050865823No Hit
AAACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCT18450.3844631061284878No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTT14310.2981933359728271No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9470.19733688970389046No Hit
AAAAAACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCT7570.15774448311071287No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6870.1431578069974369No Hit
AATGACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTT5770.12023588739086041No Hit
GAAAAACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCT5670.11815207651753527No Hit
CCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC5500.11460959803288252No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTCC253.887397E-545.027
GTTGACC406.8012014E-945.017
CGTGGTA253.887397E-545.031
GTGCAAC253.887397E-545.039
TCGTTAA207.029176E-445.025
GTACCCA253.887397E-545.033
CAAACCT207.029176E-445.042
CATATGG207.029176E-445.02
CATATCG207.029176E-445.01
GGTAATC207.029176E-445.08
CTCGTAG253.887397E-545.01
TTCAGGT253.887397E-545.032
CGAAATG253.887397E-545.01
GTACAAC207.029176E-445.012
TACCAGT351.2099554E-745.013
CGAGTAT207.029176E-445.033
TACCACG406.8012014E-945.01
TTTACGG406.8012014E-945.02
CGAAACT207.029176E-445.014
CGAAACG253.887397E-545.01