##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548794_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	479890
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.408324824438935	31.0	31.0	34.0	30.0	34.0
2	31.587176227885557	31.0	31.0	34.0	30.0	34.0
3	31.799251911896476	31.0	31.0	34.0	30.0	34.0
4	35.622486403134054	37.0	35.0	37.0	33.0	37.0
5	35.20816853862343	37.0	35.0	37.0	32.0	37.0
6	35.21262581008148	37.0	35.0	37.0	32.0	37.0
7	35.1718227093709	37.0	35.0	37.0	32.0	37.0
8	35.49228156452521	37.0	35.0	37.0	33.0	37.0
9	36.32294484152618	39.0	37.0	39.0	32.0	39.0
10	36.06435849882265	38.0	35.0	39.0	31.0	39.0
11	36.32007751776449	38.0	35.0	39.0	32.0	39.0
12	35.912786263518726	37.0	35.0	39.0	32.0	39.0
13	35.58129571360104	37.0	35.0	39.0	30.0	39.0
14	36.579826626935336	38.0	35.0	40.0	31.0	41.0
15	36.6669715976578	38.0	35.0	40.0	31.0	41.0
16	36.75117631123799	38.0	35.0	40.0	32.0	41.0
17	36.69904144699827	38.0	35.0	40.0	32.0	41.0
18	36.52250098981016	38.0	35.0	40.0	31.0	41.0
19	36.46560878534664	38.0	35.0	40.0	31.0	41.0
20	36.437452332826275	38.0	35.0	40.0	31.0	41.0
21	36.431573902352625	38.0	35.0	40.0	31.0	41.0
22	36.387019942070054	38.0	35.0	40.0	31.0	41.0
23	36.28894121569526	38.0	35.0	40.0	31.0	41.0
24	35.959976244556046	38.0	34.0	40.0	30.0	41.0
25	36.00111483881723	38.0	34.0	40.0	30.0	41.0
26	35.92496197045156	38.0	34.0	40.0	30.0	41.0
27	35.82666027631332	38.0	34.0	40.0	30.0	41.0
28	35.681101919189814	38.0	34.0	40.0	29.0	41.0
29	35.53638750547	37.0	34.0	40.0	29.0	41.0
30	35.41047740107108	37.0	34.0	40.0	29.0	41.0
31	35.26280605972202	37.0	34.0	40.0	28.0	41.0
32	35.15337264789848	37.0	34.0	40.0	27.0	41.0
33	35.125251620162956	37.0	34.0	40.0	27.0	41.0
34	34.99745775073454	37.0	34.0	40.0	27.0	41.0
35	34.89240450936673	37.0	34.0	40.0	26.0	41.0
36	34.75801537852424	37.0	34.0	40.0	26.0	41.0
37	34.62755631498885	37.0	33.0	40.0	25.0	41.0
38	34.590891662672696	37.0	33.0	40.0	25.0	41.0
39	34.53622288441101	37.0	33.0	40.0	25.0	41.0
40	34.36005959699098	36.0	33.0	40.0	24.0	41.0
41	34.41364270978766	36.0	33.0	40.0	24.0	41.0
42	34.28879743274501	36.0	33.0	40.0	24.0	41.0
43	34.18234387047032	36.0	33.0	40.0	24.0	41.0
44	33.935178895163475	36.0	33.0	40.0	23.0	41.0
45	33.7330054804226	35.0	33.0	39.0	23.0	41.0
46	33.6316072433266	35.0	33.0	39.0	23.0	41.0
47	33.50264227218737	35.0	33.0	39.0	23.0	40.0
48	33.50011044197629	35.0	33.0	39.0	23.0	40.0
49	33.34379753693555	35.0	32.0	39.0	22.0	40.0
50	33.09023942986934	35.0	32.0	39.0	21.0	40.0
51	31.075813207193317	34.0	28.0	38.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	7.0
10	6.0
11	8.0
12	6.0
13	19.0
14	42.0
15	59.0
16	113.0
17	279.0
18	498.0
19	949.0
20	1566.0
21	2328.0
22	2986.0
23	4043.0
24	4819.0
25	5319.0
26	5948.0
27	7042.0
28	8398.0
29	10441.0
30	13483.0
31	16996.0
32	22445.0
33	31023.0
34	51884.0
35	56505.0
36	47072.0
37	61703.0
38	73679.0
39	50223.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.58636354164496	34.98968513617704	19.61261955864886	25.811331763529143
2	21.63870887078289	34.282231344683154	19.761612036091606	24.31744774844235
3	19.610535747775533	33.40848944549792	21.237992039842464	25.742982766884076
4	20.759965825501677	33.96028256475442	18.18124986976182	27.09850173998208
5	17.224155535643586	37.81887515889058	17.79678676363333	27.160182541832505
6	20.784346412719582	35.97761987122049	20.417387317927023	22.820646398132904
7	78.25501677467753	9.854133238867242	6.623809623038614	5.2670403634166165
8	80.62639354852153	7.167267498801809	5.138260851445123	7.068078101231532
9	73.36702160911875	12.077351059617829	9.433411823542896	5.12221550772052
10	41.96545041572027	30.646398132905457	11.410531580153785	15.977619871220488
11	34.72712496613807	25.76965554606264	22.21488257725729	17.288336910542
12	32.10735793619371	24.11344266394382	26.104940715580653	17.674258684281817
13	24.096563795869887	34.40517618620934	22.81439496551293	18.683865052407846
14	17.333763987580486	35.27495884473525	28.732209464668983	18.659067703015275
15	14.330784138031632	28.987267915563987	34.96342911917314	21.71851882723124
16	16.845527099960407	25.87363770864156	33.76044510200254	23.520390089395487
17	16.542124236804266	26.644647731771865	27.9997499426952	28.813478088728665
18	21.890016462105898	25.900727249994794	27.10933755652337	25.099918731375944
19	22.70166079726604	30.918960595136387	25.426451895225988	20.952926712371585
20	26.371043364104274	28.630102731876057	23.47246243930901	21.526391464710663
21	21.862301777490675	30.738710954593763	22.702702702702705	24.69628456521286
22	20.44176790514493	28.907041196940963	23.475379774531664	27.17581112338244
23	20.624101356560878	30.602638104565628	21.59161474504574	27.18164579382775
24	18.301277376065347	31.14297026401884	26.824480610139823	23.73127174977599
25	19.59532392840026	28.918710537831586	26.330409052074433	25.15555648169372
26	22.024839025610035	32.479109795994916	22.668319823292837	22.82773135510221
27	19.289003730021463	30.258600929379647	25.22286357290212	25.229531767696763
28	19.513430161078578	31.090249848923712	29.665339973743983	19.730980016253724
29	21.28425264123028	29.136677155181395	26.190585342474314	23.388484861114005
30	22.30448644481027	29.057075579820374	26.113275959073956	22.5251620162954
31	27.601116922628105	28.75679843297422	20.649732230302778	22.992352414094896
32	26.855737773239703	26.220175456875534	22.326574840067515	24.59751192981725
33	26.054720873533515	25.83758778053304	24.37871178811811	23.728979557815332
34	20.686824063847965	27.131009189605955	25.30559086457313	26.876575881972954
35	22.628310654524995	25.528141865844255	28.064139698680947	23.779407780949803
36	26.732584550626186	25.15243076538373	26.710912917543606	21.404071766446478
37	20.990852070266104	28.070807893475585	26.7711350517827	24.16720498447561
38	23.840255058450897	26.63172810435725	27.17143512054846	22.356581716643397
39	22.95359353185105	24.576673821084	25.288711996499195	27.181020650565756
40	25.43207818458397	25.35393527683427	22.664568963720853	26.549417574860907
41	20.974806726541498	21.25257871595574	28.94725874679614	28.825355810706622
42	24.895705265790077	23.707307924732753	25.226406051386775	26.170580758090395
43	23.094250765800496	22.87086624018004	27.1351768113526	26.89970618266686
44	22.933380566379796	24.27514638771385	26.66423555398112	26.127237491925232
45	24.119485715476465	26.064931546812815	25.934276605055324	23.8813061326554
46	20.980224634812146	24.844443518306278	29.778282523078204	24.397049323803373
47	22.359707432953385	25.313509345891767	26.29936027006189	26.02742295109296
48	23.51893142178416	20.797891183396196	30.855404363499968	24.827773031319676
49	22.9738064973223	21.596824272229053	30.087103294504992	25.342265935943654
50	21.09587613828169	23.121965450415722	27.386692783762946	28.395465627539647
51	21.02127570901665	24.24764008418596	28.736377086415636	25.994707120381754
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4260.0
1	3498.5
2	2737.0
3	1947.0
4	1157.0
5	1098.5
6	1040.0
7	1122.0
8	1204.0
9	1293.5
10	1383.0
11	1501.5
12	1620.0
13	1703.0
14	1786.0
15	1889.5
16	1993.0
17	1867.5
18	1742.0
19	1745.5
20	1749.0
21	1743.0
22	1737.0
23	1904.0
24	2071.0
25	1782.5
26	1920.0
27	2346.0
28	2505.0
29	2664.0
30	3375.0
31	4086.0
32	4853.0
33	5620.0
34	6362.5
35	7105.0
36	8388.0
37	9671.0
38	9738.5
39	9806.0
40	13155.0
41	16504.0
42	22713.5
43	28923.0
44	37803.0
45	46683.0
46	47321.5
47	47960.0
48	48566.5
49	49173.0
50	45908.5
51	42644.0
52	38929.0
53	35214.0
54	31877.5
55	28541.0
56	26247.5
57	23954.0
58	21526.5
59	19099.0
60	18182.0
61	17265.0
62	15601.5
63	13938.0
64	12896.5
65	11855.0
66	9818.0
67	7781.0
68	6927.0
69	6073.0
70	5730.0
71	5387.0
72	4865.5
73	4344.0
74	3589.5
75	2304.0
76	1773.0
77	1491.5
78	1210.0
79	954.0
80	698.0
81	516.0
82	334.0
83	313.0
84	292.0
85	174.0
86	56.0
87	45.0
88	34.0
89	22.0
90	10.0
91	9.5
92	9.0
93	12.0
94	15.0
95	14.5
96	14.0
97	7.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	479890.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.03040290403122
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.29675875128672	23.020168555336166
2	9.181253530884458	5.330709783312701
3	3.345216959123713	2.913389883742786
4	1.5462460812035983	1.7955258690447933
5	0.9115203110931093	1.323090094312042
6	0.5696313763903806	0.9921977018034365
7	0.391363203540813	0.7953002036421525
8	0.3056484480156493	0.7098478074308912
9	0.25240053826687525	0.6594560387173565
>10	2.6784566997162504	19.018112768490965
>50	1.1173995142455122	23.487060760043796
>100	0.3947569712488972	15.215228443698217
>500	0.004314283838785762	0.8527124186103336
>1k	0.005033331145250055	3.887199671814353
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGCT	3910	0.8147700514701286	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3605	0.7512138198337119	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCC	2703	0.5632540790597845	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGT	2585	0.5386651107545479	No Hit
AATCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCT	2543	0.5299131050865823	No Hit
AAACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCT	1845	0.3844631061284878	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTT	1431	0.2981933359728271	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	947	0.19733688970389046	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCT	757	0.15774448311071287	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	687	0.1431578069974369	No Hit
AATGACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTT	577	0.12023588739086041	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCT	567	0.11815207651753527	No Hit
CCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC	550	0.11460959803288252	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0619100210464898	0.0
2	0.0	0.0	0.0	1.3899018525078664	0.0
3	0.0	0.0	0.0	1.7991623080289232	0.0
4	0.0	0.0	0.0	4.116568380253808	0.0
5	0.0	0.0	0.0	4.481235283085707	0.0
6	0.0	0.0	0.0	6.288732834607931	0.0
7	0.0	0.0	0.0	7.74510825397487	0.0
8	0.0	0.0	0.0	8.752214049052908	0.0
9	0.0	0.0	0.0	10.463647919314843	0.0
10	0.0	0.0	0.0	12.439725770489071	0.0
11	0.0	0.0	0.0	14.71358019546146	0.0
12	0.0	0.0	0.0	15.703807122465564	0.0
13	0.0	0.0	0.0	16.10869157515264	0.0
14	0.0	0.0	0.0	16.518577173935693	0.0
15	0.0	0.0	0.0	16.8480276730084	0.0
16	0.0	0.0	0.0	17.41690804142616	0.0
17	0.0	0.0	0.0	18.098939340265478	0.0
18	0.0	0.0	0.0	19.18439642418054	0.0
19	0.0	0.0	0.0	19.555106378545084	0.0
20	0.0	0.0	0.0	19.992081518681363	0.0
21	0.0	0.0	0.0	20.363624997395238	0.0
22	0.0	0.0	0.0	20.734960095021776	0.0
23	0.0	0.0	0.0	21.107962241346975	0.0
24	0.0	0.0	0.0	21.356560878534665	0.0
25	0.0	0.0	0.0	21.575361020233803	0.0
26	0.0	0.0	0.0	21.772281147763028	0.0
27	0.0	0.0	0.0	22.04817770739128	0.0
28	0.0	0.0	0.0	22.270728708662404	0.0
29	0.0	0.0	0.0	22.495571901894184	0.0
30	0.0	0.0	0.0	22.909416741336557	0.0
31	0.0	0.0	0.0	23.14509575110963	0.0
32	0.0	0.0	0.0	23.370772468690742	0.0
33	0.0	0.0	0.0	23.59707432953385	0.0
34	0.0	0.0	0.0	23.788993310967097	0.0
35	0.0	0.0	0.0	24.034882994019462	0.0
36	0.0	0.0	0.0	24.240763508303985	0.0
37	0.0	0.0	0.0	24.441851257579863	0.0
38	0.0	0.0	0.0	24.653358061222363	0.0
39	0.0	0.0	0.0	24.87611744358082	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTCC	25	3.887397E-5	45.0	27
GTTGACC	40	6.8012014E-9	45.0	17
CGTGGTA	25	3.887397E-5	45.0	31
GTGCAAC	25	3.887397E-5	45.0	39
TCGTTAA	20	7.029176E-4	45.0	25
GTACCCA	25	3.887397E-5	45.0	33
CAAACCT	20	7.029176E-4	45.0	42
CATATGG	20	7.029176E-4	45.0	2
CATATCG	20	7.029176E-4	45.0	1
GGTAATC	20	7.029176E-4	45.0	8
CTCGTAG	25	3.887397E-5	45.0	1
TTCAGGT	25	3.887397E-5	45.0	32
CGAAATG	25	3.887397E-5	45.0	1
GTACAAC	20	7.029176E-4	45.0	12
TACCAGT	35	1.2099554E-7	45.0	13
CGAGTAT	20	7.029176E-4	45.0	33
TACCACG	40	6.8012014E-9	45.0	1
TTTACGG	40	6.8012014E-9	45.0	2
CGAAACT	20	7.029176E-4	45.0	14
CGAAACG	25	3.887397E-5	45.0	1
>>END_MODULE