FastQCFastQC Report
Sat 18 Jun 2016
SRR3548793_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548793_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487029
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38240.7851688503148683No Hit
CTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGCT37400.7679214174104623No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCC26510.5443207693997688No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGT24400.5009968605565582No Hit
AATCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCT24070.494221083344113No Hit
AAACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCT16810.3451539846703174No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTT12250.25152506318925566No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9550.19608688599652177No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7950.16323463284527207No Hit
AAAAAACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCT6780.1392114227284207No Hit
AATGACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTT5350.10984972147449125No Hit
GAAAAACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCT4990.10245796451546006No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA207.029277E-445.00000442
TACCACC207.029277E-445.00000433
CTATCGA207.029277E-445.0000041
CGGCCTA207.029277E-445.00000445
ATCAGTA207.029277E-445.00000445
CGGCATG207.029277E-445.00000437
CGGTTCG207.029277E-445.00000430
TAGAACG207.029277E-445.00000414
ATTACGC207.029277E-445.00000416
TAGCGCG406.8012014E-945.0000041
TAGCGCA207.029277E-445.00000424
GGCAACC207.029277E-445.00000427
TTCACGA207.029277E-445.00000423
GCAAACG800.045.0000041
CGCGCGA207.029277E-445.00000414
CCCAGAC453.8380676E-1045.00000421
GTCTAGT207.029277E-445.00000426
CAATTGG406.8012014E-945.0000042
CAATTCA207.029277E-445.00000444
ACTTATG207.029277E-445.0000041