##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548793_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	487029
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.199242755564864	31.0	31.0	34.0	28.0	34.0
2	31.406850516088365	31.0	31.0	34.0	28.0	34.0
3	31.40612160672157	31.0	31.0	34.0	28.0	34.0
4	35.40725295618947	37.0	35.0	37.0	33.0	37.0
5	35.06113598984865	37.0	35.0	37.0	32.0	37.0
6	35.1169211689653	37.0	35.0	37.0	32.0	37.0
7	35.03004954530429	37.0	35.0	37.0	32.0	37.0
8	35.41049095639069	37.0	35.0	37.0	33.0	37.0
9	35.850546887351676	39.0	37.0	39.0	32.0	39.0
10	35.74887943017767	38.0	35.0	39.0	30.0	39.0
11	36.14541433877654	38.0	35.0	39.0	32.0	39.0
12	35.80012073203033	37.0	35.0	39.0	31.0	39.0
13	35.43818540579719	37.0	35.0	39.0	30.0	39.0
14	36.42805253896585	38.0	35.0	40.0	31.0	41.0
15	36.54988306651144	38.0	35.0	40.0	31.0	41.0
16	36.63354338242692	38.0	35.0	40.0	31.0	41.0
17	36.583959066092575	38.0	35.0	40.0	31.0	41.0
18	36.442866851871244	38.0	35.0	40.0	31.0	41.0
19	36.381137468200045	38.0	35.0	40.0	31.0	41.0
20	36.32548985789347	38.0	35.0	40.0	31.0	41.0
21	36.29041186459123	38.0	35.0	40.0	31.0	41.0
22	36.2796630180133	38.0	35.0	40.0	30.0	41.0
23	36.1895287549612	38.0	35.0	40.0	31.0	41.0
24	35.83117637758737	38.0	34.0	40.0	30.0	41.0
25	35.91282038646569	38.0	34.0	40.0	30.0	41.0
26	35.8315808709543	38.0	34.0	40.0	30.0	41.0
27	35.714144332267686	38.0	34.0	40.0	30.0	41.0
28	35.54131273497061	37.0	34.0	40.0	29.0	41.0
29	35.40853419406237	37.0	34.0	40.0	29.0	41.0
30	35.31702218964374	37.0	34.0	40.0	28.0	41.0
31	35.24174125154765	37.0	34.0	40.0	28.0	41.0
32	35.10991131944915	37.0	34.0	40.0	27.0	41.0
33	35.03783552930113	37.0	34.0	40.0	27.0	41.0
34	34.98015723909665	37.0	34.0	40.0	27.0	41.0
35	34.84939911175721	37.0	34.0	40.0	26.0	41.0
36	34.73750228425823	37.0	33.0	40.0	26.0	41.0
37	34.57794915703172	37.0	33.0	40.0	25.0	41.0
38	34.56164006660794	36.0	33.0	40.0	25.0	41.0
39	34.453348773892316	36.0	33.0	40.0	25.0	41.0
40	34.23404150471532	36.0	33.0	40.0	24.0	41.0
41	34.29553886934864	36.0	33.0	40.0	24.0	41.0
42	34.22761067616097	36.0	33.0	40.0	24.0	41.0
43	34.15895562687232	36.0	33.0	40.0	24.0	41.0
44	34.02141145599133	36.0	33.0	40.0	23.0	41.0
45	33.85360830669221	35.0	33.0	39.0	23.0	41.0
46	33.63652267113457	35.0	33.0	39.0	23.0	41.0
47	33.491110385623855	35.0	33.0	39.0	23.0	40.0
48	33.430980496027956	35.0	33.0	39.0	23.0	40.0
49	33.282556069556435	35.0	32.0	39.0	22.0	40.0
50	33.14214553958799	35.0	32.0	39.0	22.0	40.0
51	31.279324229152678	34.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	4.0
10	9.0
11	16.0
12	8.0
13	15.0
14	33.0
15	67.0
16	139.0
17	250.0
18	521.0
19	1010.0
20	1691.0
21	2398.0
22	3258.0
23	4068.0
24	4783.0
25	5385.0
26	6248.0
27	7399.0
28	8891.0
29	11151.0
30	14038.0
31	17973.0
32	23781.0
33	32557.0
34	53581.0
35	56838.0
36	49280.0
37	62868.0
38	72372.0
39	46391.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.862677581827775	34.59403854801254	19.588155941432646	25.955127928727034
2	21.826831667108117	34.028158487482266	19.763710169209638	24.38129967619998
3	20.113586665270446	33.19165799161857	21.009015890224198	25.685739452886793
4	20.831408396625253	33.94705448751512	17.987429906638003	27.234107209221627
5	17.19158407404898	37.71910912902517	17.831587030751763	27.257719766174088
6	20.70020471060245	36.14343293725836	20.306388325951843	22.849974026187354
7	78.70886538583945	9.880315135238353	6.270673820244791	5.140145658677409
8	81.11652488866166	7.239815288206657	4.908537273961098	6.735122549170583
9	73.25292744374565	12.976639994743639	8.885918497666463	4.8845140638442475
10	42.53668672707375	30.794264817906118	11.094821868923617	15.574226586096515
11	35.25909134774315	25.867248151547447	21.750039525367075	17.12362097534233
12	32.55658287288847	24.347831443302145	25.67916900225654	17.416416681552843
13	24.181516911723943	34.682944958103114	22.60214484147761	18.533393288695336
14	17.41703266129943	35.877945666479825	28.178404160737863	18.52661751148289
15	14.510224237160415	28.80547154276234	35.176755388282835	21.50754883179441
16	17.129986099390386	25.649601974420417	33.942742629289015	23.277669296900186
17	16.609277886943076	26.437029417139428	27.897517396294674	29.056175299622815
18	22.122501945469367	26.00892349326221	27.05280383714317	24.815770724125258
19	23.050578096992172	30.761617891337067	25.25927614166713	20.928527870003634
20	26.883409406832037	28.412887117604907	23.24379041083796	21.459913064725097
21	22.206275191005055	30.756279400199986	22.61939227438202	24.41805313441294
22	20.593024234696497	28.76502220606986	23.37827932217589	27.26367423705775
23	20.564073186606958	30.734104129322894	21.629102168454033	27.072720515616112
24	18.522510979838984	31.10636122284299	26.95239913844966	23.418728658868364
25	19.752006554024504	28.99437199838203	26.31095889567151	24.94266255192196
26	21.842436487354963	32.538103480490896	22.849768699605157	22.769691332548987
27	19.44750723262886	30.481963086387054	25.138954764500674	24.931574916483413
28	19.332319020017287	31.282326103784374	29.82224877779352	19.563106098404816
29	21.076978988930843	29.23070289448883	26.470497650037267	23.22182046654306
30	22.293744315020255	29.191074864125135	26.136020647641107	22.379160173213506
31	27.87246755326685	28.83154801870115	20.575571475209895	22.720412952822112
32	26.92919723466159	26.456535442447986	22.43808890230356	24.176178420586865
33	26.105221660311813	26.04998880970127	24.168170683881247	23.676618846105672
34	20.747019171342977	27.40206435345739	25.292333721400574	26.558582753799055
35	22.6682600009445	25.671982571879703	27.80676304696435	23.852994380211445
36	26.805179979015627	25.517166328904437	26.510125680400964	21.167528011678975
37	20.994643029470524	28.492964484661076	26.586712495559812	23.925679990308584
38	23.662245985352	26.965540039710163	26.813393042303435	22.5588209326344
39	22.94935209196988	24.97900535697053	25.072839605033785	26.9988029460258
40	25.458648252978776	25.565212749138144	22.643620811081064	26.332518186802016
41	21.004704031998095	21.518841793815152	28.69295257571931	28.783501598467442
42	24.910015625352905	23.888515878931234	25.157023503733868	26.044444991981997
43	23.119157175445405	23.15693726656934	26.7975828954744	26.926322662510856
44	22.88159431984543	24.557675210305753	26.59862143732714	25.96210903252168
45	24.053187797851873	26.475014834845563	25.764790187031984	23.70700718027058
46	21.044537388943986	25.340380141634277	29.40933702099875	24.205745448422988
47	22.39476499346035	25.604430126337448	26.208911584320443	25.791893295881763
48	23.5702596765285	21.21906498381	30.43822852437945	24.77244681528205
49	23.031072071683617	22.086569793585188	29.857564949931113	25.02479318480008
50	21.10654601676697	23.60762911448805	27.40432294586154	27.881501922883444
51	21.167528011678975	24.847391017783334	28.32726593282946	25.657815037708225
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4476.0
1	3659.0
2	2842.0
3	2026.0
4	1210.0
5	1101.5
6	993.0
7	1098.0
8	1203.0
9	1281.0
10	1359.0
11	1529.5
12	1700.0
13	1809.0
14	1918.0
15	1930.0
16	1942.0
17	1897.0
18	1852.0
19	1822.5
20	1793.0
21	1751.0
22	1709.0
23	1914.0
24	2119.0
25	1870.0
26	1978.5
27	2336.0
28	2551.5
29	2767.0
30	3473.0
31	4179.0
32	5001.0
33	5823.0
34	6667.0
35	7511.0
36	8750.5
37	9990.0
38	10125.5
39	10261.0
40	13819.5
41	17378.0
42	23422.5
43	29467.0
44	38088.5
45	46710.0
46	47462.5
47	48215.0
48	48690.5
49	49166.0
50	45598.5
51	42031.0
52	38782.0
53	35533.0
54	32116.5
55	28700.0
56	26584.5
57	24469.0
58	21907.5
59	19346.0
60	18480.0
61	17614.0
62	16272.5
63	14931.0
64	13491.5
65	12052.0
66	10069.0
67	8086.0
68	7176.5
69	6267.0
70	5922.0
71	5577.0
72	5006.0
73	4435.0
74	3740.5
75	2425.0
76	1804.0
77	1498.0
78	1192.0
79	972.0
80	752.0
81	511.5
82	271.0
83	266.5
84	262.0
85	160.5
86	59.0
87	41.0
88	23.0
89	18.5
90	14.0
91	12.5
92	11.0
93	10.0
94	9.0
95	6.5
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	487029.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.286882616856097
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.4856320175006	23.27886374623159
2	9.051544668107974	5.301830523922159
3	3.3058387784315677	2.904531367625289
4	1.5794298969020621	1.8502631196849528
5	0.9083511779792983	1.3301387162181335
6	0.6052810199107811	1.0636076508202794
7	0.4244309854982417	0.8701182315870492
8	0.29012142448128286	0.679740168273473
9	0.21879170147129623	0.5766954190678867
>10	2.6379837676248403	18.95223037874306
>50	1.074809228400052	22.796714876770483
>100	0.41002243053212756	16.069185191382903
>500	0.002822873876296919	0.6124015501629307
>1k	0.004940029283519609	3.713679059509801
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3824	0.7851688503148683	No Hit
CTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGCT	3740	0.7679214174104623	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCC	2651	0.5443207693997688	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGT	2440	0.5009968605565582	No Hit
AATCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCT	2407	0.494221083344113	No Hit
AAACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCT	1681	0.3451539846703174	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTT	1225	0.25152506318925566	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	955	0.19608688599652177	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	795	0.16323463284527207	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCT	678	0.1392114227284207	No Hit
AATGACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTT	535	0.10984972147449125	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCT	499	0.10245796451546006	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.106531643906215E-4	0.0	0.0	0.9919327185855462	0.0
2	4.106531643906215E-4	0.0	0.0	1.2650170729053096	0.0
3	4.106531643906215E-4	0.0	0.0	1.6109923639044081	0.0
4	4.106531643906215E-4	0.0	0.0	3.703680889639015	0.0
5	4.106531643906215E-4	0.0	0.0	4.035283319884442	0.0
6	4.106531643906215E-4	0.0	0.0	5.669272260994725	0.0
7	4.106531643906215E-4	0.0	0.0	6.991370123750331	0.0
8	4.106531643906215E-4	0.0	0.0	7.936899034759737	0.0
9	4.106531643906215E-4	0.0	0.0	9.502308897416786	0.0
10	4.106531643906215E-4	0.0	0.0	11.369548835900943	0.0
11	4.106531643906215E-4	0.0	0.0	13.484001979348252	0.0
12	4.106531643906215E-4	0.0	0.0	14.388054920754206	0.0
13	6.159797465859323E-4	0.0	0.0	14.783513918062374	0.0
14	6.159797465859323E-4	0.0	0.0	15.183284773596645	0.0
15	6.159797465859323E-4	0.0	0.0	15.492301279800587	0.0
16	6.159797465859323E-4	0.0	0.0	16.059413299824037	0.0
17	6.159797465859323E-4	0.0	0.0	16.71070921854756	0.0
18	6.159797465859323E-4	0.0	0.0	17.740832681421434	0.0
19	6.159797465859323E-4	0.0	0.0	18.077978929386134	0.0
20	6.159797465859323E-4	0.0	0.0	18.482677622893092	0.0
21	6.159797465859323E-4	0.0	0.0	18.825778341741458	0.0
22	6.159797465859323E-4	0.0	0.0	19.19351825045326	0.0
23	6.159797465859323E-4	0.0	0.0	19.551813136384077	0.0
24	6.159797465859323E-4	0.0	0.0	19.79902634134723	0.0
25	6.159797465859323E-4	0.0	0.0	19.99552388050814	0.0
26	6.159797465859323E-4	0.0	0.0	20.197770563970522	0.0
27	6.159797465859323E-4	0.0	0.0	20.455660751207834	0.0
28	6.159797465859323E-4	0.0	0.0	20.678850746054135	0.0
29	6.159797465859323E-4	0.0	0.0	20.89464898394141	0.0
30	6.159797465859323E-4	0.0	0.0	21.29113461416055	0.0
31	6.159797465859323E-4	0.0	0.0	21.525412244445402	0.0
32	6.159797465859323E-4	0.0	0.0	21.745111687394385	0.0
33	6.159797465859323E-4	0.0	0.0	21.952696861993843	0.0
34	6.159797465859323E-4	0.0	0.0	22.173012284689413	0.0
35	6.159797465859323E-4	0.0	0.0	22.411601773200363	0.0
36	6.159797465859323E-4	0.0	0.0	22.6070726794503	0.0
37	6.159797465859323E-4	0.0	0.0	22.795562481905595	0.0
38	6.159797465859323E-4	0.0	0.0	22.997398512203585	0.0
39	6.159797465859323E-4	0.0	0.0	23.20559966654963	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTA	20	7.029277E-4	45.000004	42
TACCACC	20	7.029277E-4	45.000004	33
CTATCGA	20	7.029277E-4	45.000004	1
CGGCCTA	20	7.029277E-4	45.000004	45
ATCAGTA	20	7.029277E-4	45.000004	45
CGGCATG	20	7.029277E-4	45.000004	37
CGGTTCG	20	7.029277E-4	45.000004	30
TAGAACG	20	7.029277E-4	45.000004	14
ATTACGC	20	7.029277E-4	45.000004	16
TAGCGCG	40	6.8012014E-9	45.000004	1
TAGCGCA	20	7.029277E-4	45.000004	24
GGCAACC	20	7.029277E-4	45.000004	27
TTCACGA	20	7.029277E-4	45.000004	23
GCAAACG	80	0.0	45.000004	1
CGCGCGA	20	7.029277E-4	45.000004	14
CCCAGAC	45	3.8380676E-10	45.000004	21
GTCTAGT	20	7.029277E-4	45.000004	26
CAATTGG	40	6.8012014E-9	45.000004	2
CAATTCA	20	7.029277E-4	45.000004	44
ACTTATG	20	7.029277E-4	45.000004	1
>>END_MODULE