##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548791_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1085618
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.083132372528826	31.0	31.0	34.0	28.0	34.0
2	31.29173152987515	31.0	31.0	34.0	30.0	34.0
3	31.163652408121457	31.0	31.0	34.0	28.0	34.0
4	35.13040038024425	37.0	35.0	37.0	32.0	37.0
5	34.77497056975842	35.0	35.0	37.0	32.0	37.0
6	34.81101916143616	35.0	35.0	37.0	32.0	37.0
7	34.738228363936486	35.0	35.0	37.0	32.0	37.0
8	35.15984351770144	37.0	35.0	37.0	32.0	37.0
9	35.787228104176606	39.0	35.0	39.0	32.0	39.0
10	35.453053468162835	37.0	35.0	39.0	30.0	39.0
11	35.95263250977784	37.0	35.0	39.0	31.0	39.0
12	35.77169685837928	37.0	35.0	39.0	30.0	39.0
13	35.84425276662694	37.0	35.0	39.0	31.0	39.0
14	36.7771269452054	38.0	35.0	40.0	31.0	41.0
15	36.85321908811387	38.0	35.0	40.0	31.0	41.0
16	36.65799480111789	38.0	35.0	40.0	31.0	41.0
17	36.623058939700705	38.0	35.0	40.0	31.0	41.0
18	36.37105409084963	38.0	35.0	40.0	30.0	41.0
19	36.36155443259047	38.0	35.0	40.0	30.0	41.0
20	36.56486812119917	38.0	35.0	40.0	31.0	41.0
21	36.799447872087605	38.0	35.0	40.0	31.0	41.0
22	36.8204027567708	38.0	35.0	40.0	31.0	41.0
23	36.78203290660251	38.0	35.0	40.0	31.0	41.0
24	36.347743865705986	38.0	35.0	40.0	30.0	41.0
25	36.4555709282639	38.0	35.0	40.0	30.0	41.0
26	36.395003583212514	38.0	35.0	40.0	30.0	41.0
27	36.31868207785796	38.0	35.0	40.0	30.0	41.0
28	36.280260644167655	38.0	35.0	40.0	30.0	41.0
29	35.9806699962602	38.0	35.0	40.0	30.0	41.0
30	35.858183080973234	38.0	35.0	40.0	29.0	41.0
31	35.93564034494638	38.0	35.0	40.0	30.0	41.0
32	35.55344237107344	38.0	34.0	40.0	28.0	41.0
33	35.71854556575149	38.0	34.0	40.0	29.0	41.0
34	35.80142923201347	38.0	35.0	40.0	29.0	41.0
35	35.42086535042713	38.0	34.0	40.0	27.0	41.0
36	35.60469796926728	38.0	34.0	40.0	29.0	41.0
37	34.93627408535968	38.0	33.0	40.0	25.0	41.0
38	35.152145598175416	38.0	33.0	40.0	27.0	41.0
39	34.829362630317476	37.0	33.0	40.0	25.0	41.0
40	34.683209010904385	37.0	33.0	40.0	25.0	41.0
41	35.000675191457766	38.0	34.0	40.0	26.0	41.0
42	35.00873143223491	38.0	34.0	40.0	26.0	41.0
43	35.03769465871052	38.0	34.0	40.0	26.0	41.0
44	34.98564227932846	37.0	34.0	40.0	26.0	41.0
45	34.70670622631533	37.0	33.0	40.0	26.0	41.0
46	34.32537872437634	37.0	33.0	40.0	24.0	41.0
47	34.273596237350525	37.0	33.0	40.0	24.0	41.0
48	34.21319469647703	37.0	33.0	40.0	24.0	41.0
49	34.07571539897091	36.0	32.0	40.0	24.0	41.0
50	33.945949680274275	36.0	32.0	40.0	23.0	41.0
51	31.755877297539282	35.0	29.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	10.0
10	7.0
11	9.0
12	7.0
13	13.0
14	17.0
15	58.0
16	114.0
17	294.0
18	568.0
19	1143.0
20	2069.0
21	3231.0
22	4581.0
23	6168.0
24	7666.0
25	9351.0
26	11706.0
27	15202.0
28	19610.0
29	25422.0
30	33265.0
31	42825.0
32	54822.0
33	71223.0
34	99527.0
35	112181.0
36	116971.0
37	146447.0
38	175716.0
39	125387.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.434401419283763	41.703619505203484	17.90611430540024	22.95586477011251
2	19.591053206560687	41.5009699544407	18.449307214876686	20.458669624121928
3	18.74342540377923	26.357245366233794	32.029774745812986	22.86955448417399
4	27.19630661982392	28.075437216405767	18.313163562136957	26.415092601633354
5	15.488320937935812	40.038669218822825	19.37209957830471	25.100910264936655
6	20.71695568791232	32.45340442033938	27.40678581232072	19.42285407942757
7	71.78731376966853	13.24913551543913	8.956004782529398	6.007545932362949
8	73.96855984333347	4.049951271994385	5.890285533217025	16.091203351455118
9	70.4600513256044	10.462888419315082	11.564565068007347	7.512495187073169
10	31.765961876092696	37.6295345139819	14.859554649978168	15.744948959947239
11	24.305326551328367	21.42972942600436	34.072758557798416	20.19218546486886
12	23.980718816379245	21.66563192577868	38.77726787875662	15.576381379085461
13	23.551378109058618	30.793796713024285	26.105223015830614	19.54960216208648
14	15.67982476340665	30.002450217295586	37.79791786797935	16.519807151318417
15	13.787906980171662	30.944586401478237	29.72887332376582	25.538633294584283
16	16.06062169197637	26.42872538959376	34.99186638393984	22.518786534490033
17	14.922376010714633	27.720063595113565	30.68501074963753	26.672549644534264
18	16.50442420814688	27.86790565373824	29.525302638681378	26.102367499433505
19	17.464890965330344	31.91288280039572	32.38063480892911	18.24159142534483
20	20.24330841972038	33.628771814763574	27.14039376649982	18.987525999016228
21	17.011877105943345	27.626015780873196	29.116411113301368	26.245695999882095
22	17.25837265041663	30.590870821964998	29.108857811863842	23.041898715754527
23	21.0248908916396	27.766672991789008	22.9335733195286	28.274862797042786
24	15.423841535420376	37.61101971411675	25.913442850063284	21.05169590039959
25	19.296013883336496	26.005556282228188	27.20542584960824	27.493003984827073
26	23.69599619755752	32.28603431409575	21.213907654441986	22.804061833904743
27	16.964991368971404	27.96766450077283	29.720214661142318	25.347129469113444
28	27.322962589050658	25.60200733591374	25.372276436094467	21.70275363894114
29	23.01638329504485	30.033860897663818	27.34000357400117	19.609752233290163
30	24.98457100011238	28.772091103868945	24.365752962828545	21.87758493319013
31	30.898529685395783	29.259371160021296	19.898619956559305	19.943479198023613
32	21.08964663445153	32.40541332218147	21.82019826495139	24.68474177841561
33	28.43827202570333	25.482720441260188	26.60125384803863	19.477753684997854
34	22.315123735973426	26.99586779143308	21.887256843567442	28.801751629026047
35	23.810032626577673	31.853838090378016	24.971583006177127	19.364546276867188
36	28.60241816182119	24.0614101829557	28.329486062316576	19.00668559290653
37	19.77177976046823	35.019408300157146	21.73582236108834	23.472989578286285
38	27.094521277281697	27.16858047674228	27.376204152841975	18.36069409313405
39	18.425634062810307	28.72704763554031	28.117901508633793	24.72941679301559
40	20.050883459927892	30.688603173491963	27.281511544576453	21.97900182200369
41	19.040583335943214	22.203758596486058	33.36449837788246	25.39115968968827
42	25.634062810307125	28.006260028849926	22.12325145677393	24.23642570406902
43	22.210482877034096	24.33130253919887	28.63032853176716	24.827886051999876
44	20.438865236206475	23.761304620962438	26.31432050684495	29.48550963598614
45	28.385951596233667	27.783345523010855	24.5440845674998	19.286618313255676
46	18.73402983369841	23.713774089965348	36.971291927731485	20.58090414860476
47	21.9731986757773	27.544587506839424	24.54951925999753	25.932694557385748
48	19.229139531584774	21.649788415446316	35.66300485069333	23.458067202275572
49	22.47669069599067	21.79238000843759	30.138409643170988	25.592519652400753
50	20.453142818192035	22.44758285142656	26.39584089431089	30.703433436070515
51	18.301557269684178	22.57156753112052	34.65850787293505	24.46836732626025
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4473.0
1	3546.5
2	2620.0
3	1904.5
4	1189.0
5	1154.0
6	1119.0
7	1254.5
8	1390.0
9	1573.0
10	1756.0
11	1968.0
12	2180.0
13	2346.5
14	2513.0
15	2527.0
16	2541.0
17	2519.5
18	2498.0
19	2652.0
20	2806.0
21	3106.5
22	3407.0
23	4041.0
24	4675.0
25	4889.5
26	6949.0
27	8794.0
28	11013.5
29	13233.0
30	15070.0
31	16907.0
32	19962.0
33	23017.0
34	25961.0
35	28905.0
36	30472.5
37	32040.0
38	34783.5
39	37527.0
40	41495.5
41	45464.0
42	55737.5
43	66011.0
44	117337.5
45	168664.0
46	160825.5
47	152987.0
48	136075.0
49	119163.0
50	105240.0
51	91317.0
52	78923.5
53	66530.0
54	57039.5
55	47549.0
56	41679.5
57	35810.0
58	31503.0
59	27196.0
60	23783.5
61	20371.0
62	17847.5
63	15324.0
64	13045.5
65	10767.0
66	8622.5
67	6478.0
68	5391.5
69	4305.0
70	3572.5
71	2840.0
72	2516.5
73	2193.0
74	1773.5
75	1149.5
76	945.0
77	823.0
78	701.0
79	564.5
80	428.0
81	327.0
82	226.0
83	205.5
84	185.0
85	111.0
86	37.0
87	31.5
88	26.0
89	24.0
90	22.0
91	17.5
92	13.0
93	9.0
94	5.0
95	9.0
96	13.0
97	6.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1085618.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.473919681638254
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.21098796529255	20.44077493933093
2	9.634090977787583	5.101043015030885
3	3.5522383191833478	2.8212501585629286
4	1.7037927821734484	1.804242930776594
5	0.9944270273519495	1.31631906256829
6	0.6838665332462003	1.0862776604472182
7	0.5196293899444623	0.9629638713525864
8	0.41093669150264744	0.8703283972063396
9	0.3504284004378043	0.8349491994619858
>10	3.740035151019224	22.96921756616963
>50	0.9391486095267629	17.259650076668432
>100	0.2461290906666398	9.334905478430489
>500	0.00928789021383543	1.7109942548731494
>1k	0.0025005858268018467	1.4972618308175822
>5k	0.0010716796400579342	1.833937684639626
>10k+	0.0014289061867439124	10.155883873663374
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCT	36144	3.3293478921683315	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCC	32273	2.972776796257984	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGT	28186	2.5963091990000167	No Hit
CTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCTGCT	10785	0.993443365898502	No Hit
AATGACTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTT	7228	0.6657958876879344	No Hit
AACTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCTG	6300	0.5803146226389024	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCT	5864	0.5401531662150039	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3822	0.35205753773426746	No Hit
AATGCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTC	3372	0.3106064932600602	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGGAGATCATCGTAT	3250	0.29936865453594175	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1761	0.16221175404239796	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGAGATCATC	1293	0.11910266778922236	No Hit
AAACTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCT	1292	0.11901055435705746	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8422686432981031E-4	0.0	0.0	1.339145076813391	0.0
2	1.8422686432981031E-4	0.0	0.0	1.577166185527506	0.0
3	1.8422686432981031E-4	0.0	0.0	2.621456165981036	0.0
4	1.8422686432981031E-4	0.0	0.0	7.802007704367466	0.0
5	1.8422686432981031E-4	0.0	0.0	8.424049711777071	0.0
6	1.8422686432981031E-4	0.0	0.0	10.295610426503613	0.0
7	1.8422686432981031E-4	0.0	0.0	11.8093104572695	0.0
8	1.8422686432981031E-4	0.0	0.0	12.612171132018814	0.0
9	1.8422686432981031E-4	0.0	0.0	16.692151382898956	0.0
10	1.8422686432981031E-4	0.0	0.0	18.148372631993944	0.0
11	1.8422686432981031E-4	0.0	0.0	23.113931419707484	0.0
12	1.8422686432981031E-4	0.0	0.0	23.982468971590375	0.0
13	1.8422686432981031E-4	0.0	0.0	24.507515534930334	0.0
14	1.8422686432981031E-4	0.0	0.0	25.210248908916395	0.0
15	1.8422686432981031E-4	0.0	0.0	25.567188458555403	0.0
16	1.8422686432981031E-4	0.0	0.0	26.09334038308134	0.0
17	1.8422686432981031E-4	0.0	0.0	26.763373488648863	0.0
18	1.8422686432981031E-4	0.0	0.0	27.766580878356844	0.0
19	1.8422686432981031E-4	0.0	0.0	28.151338684509653	0.0
20	1.8422686432981031E-4	0.0	0.0	28.524766538506178	0.0
21	1.8422686432981031E-4	0.0	0.0	28.923525586348052	0.0
22	1.8422686432981031E-4	0.0	0.0	29.370552072644337	0.0
23	1.8422686432981031E-4	0.0	0.0	29.803577317251555	0.0
24	1.8422686432981031E-4	0.0	0.0	30.12044752389883	0.0
25	1.8422686432981031E-4	0.0	0.0	30.3835234861618	0.0
26	1.8422686432981031E-4	0.0	0.0	30.64291491113817	0.0
27	1.8422686432981031E-4	0.0	0.0	30.92570314788443	0.0
28	1.8422686432981031E-4	0.0	0.0	31.201767104082652	0.0
29	1.8422686432981031E-4	0.0	0.0	31.511820916749723	0.0
30	1.8422686432981031E-4	0.0	0.0	31.982336328248056	0.0
31	1.8422686432981031E-4	0.0	0.0	32.269638123170395	0.0
32	1.8422686432981031E-4	0.0	0.0	32.54017527343872	0.0
33	1.8422686432981031E-4	0.0	0.0	32.82572691314993	0.0
34	1.8422686432981031E-4	0.0	0.0	33.09046091719187	0.0
35	1.8422686432981031E-4	0.0	0.0	33.41202890887955	0.0
36	1.8422686432981031E-4	0.0	0.0	33.69969915753055	0.0
37	1.8422686432981031E-4	0.0	0.0	33.98340852859846	0.0
38	1.8422686432981031E-4	0.0	0.0	34.263801816108426	0.0
39	1.8422686432981031E-4	0.0	0.0	34.5407869066283	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCAA	20	7.0331123E-4	45.000004	42
ACCGGAT	20	7.0331123E-4	45.000004	4
GTCGAGA	20	7.0331123E-4	45.000004	12
GATCGCG	20	7.0331123E-4	45.000004	21
GCGTTAG	20	7.0331123E-4	45.000004	1
CGTTCAC	20	7.0331123E-4	45.000004	21
ATCGTAC	20	7.0331123E-4	45.000004	28
TGTTGCG	20	7.0331123E-4	45.000004	1
ACGATTG	20	7.0331123E-4	45.000004	1
TACGACT	20	7.0331123E-4	45.000004	14
TTGCGAT	20	7.0331123E-4	45.000004	24
CGTACTC	20	7.0331123E-4	45.000004	30
ATGCGTC	20	7.0331123E-4	45.000004	34
TTACGTC	20	7.0331123E-4	45.000004	20
ATACCCG	40	6.8139343E-9	45.000004	1
TAGTACG	20	7.0331123E-4	45.000004	1
CGAACTA	25	3.8906634E-5	45.0	1
CGACGGT	25	3.8906634E-5	45.0	28
ACGTTGT	25	3.8906634E-5	45.0	17
TTCGTAC	25	3.8906634E-5	45.0	33
>>END_MODULE