Basic Statistics
Measure | Value |
---|---|
Filename | SRR3548787_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 605452 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AATGATACGGCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCC | 14306 | 2.3628627868105148 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGT | 12077 | 1.9947080858598205 | No Hit |
AATCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCT | 12024 | 1.9859542953033438 | No Hit |
CTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGCT | 5512 | 0.9103942178735885 | TruSeq Adapter, Index 16 (95% over 22bp) |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 4199 | 0.6935314442763423 | No Hit |
AATGACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTT | 2859 | 0.47220919247107945 | No Hit |
AATGATCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCT | 2373 | 0.3919385847267826 | No Hit |
AAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCT | 1930 | 0.31877010894340096 | No Hit |
AACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTG | 1769 | 0.2921784055548582 | No Hit |
AATGATACGGCGACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTAT | 1485 | 0.24527130144090695 | No Hit |
AAAAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTT | 1428 | 0.2358568474462055 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1058 | 0.17474547941042395 | No Hit |
AATGCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTC | 874 | 0.14435496125208935 | No Hit |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 824 | 0.13609666827428105 | No Hit |
AAAAAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCT | 745 | 0.12304856536934389 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGGGTAC | 55 | 1.8189894E-12 | 45.000004 | 6 |
AATCCGA | 25 | 3.8886104E-5 | 45.000004 | 2 |
CCCCTAG | 25 | 3.8886104E-5 | 45.000004 | 32 |
AATTTCG | 25 | 3.8886104E-5 | 45.000004 | 37 |
CACGGAG | 25 | 3.8886104E-5 | 45.000004 | 4 |
CTATCAT | 25 | 3.8886104E-5 | 45.000004 | 34 |
TATGCGT | 25 | 3.8886104E-5 | 45.000004 | 41 |
ATCGTTC | 25 | 3.8886104E-5 | 45.000004 | 41 |
ATCGTGC | 25 | 3.8886104E-5 | 45.000004 | 29 |
ATCCGGC | 50 | 2.1827873E-11 | 45.000004 | 5 |
ATATACG | 50 | 2.1827873E-11 | 45.000004 | 1 |
GTATGCG | 25 | 3.8886104E-5 | 45.000004 | 40 |
TACTGCC | 25 | 3.8886104E-5 | 45.000004 | 38 |
TTGGTAC | 25 | 3.8886104E-5 | 45.000004 | 44 |
CAAGCGT | 25 | 3.8886104E-5 | 45.000004 | 17 |
GCCTAAA | 25 | 3.8886104E-5 | 45.000004 | 14 |
TAGATTA | 25 | 3.8886104E-5 | 45.000004 | 29 |
TATAGCC | 25 | 3.8886104E-5 | 45.000004 | 34 |
GCAATTT | 25 | 3.8886104E-5 | 45.000004 | 35 |
CGGTCCG | 25 | 3.8886104E-5 | 45.000004 | 29 |