FastQCFastQC Report
Sat 18 Jun 2016
SRR3548787_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548787_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences605452
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCC143062.3628627868105148No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGT120771.9947080858598205No Hit
AATCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCT120241.9859542953033438No Hit
CTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGCT55120.9103942178735885TruSeq Adapter, Index 16 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41990.6935314442763423No Hit
AATGACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTT28590.47220919247107945No Hit
AATGATCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCT23730.3919385847267826No Hit
AAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCT19300.31877010894340096No Hit
AACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTG17690.2921784055548582No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTAT14850.24527130144090695No Hit
AAAAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTT14280.2358568474462055No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10580.17474547941042395No Hit
AATGCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTC8740.14435496125208935No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8240.13609666827428105No Hit
AAAAAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCT7450.12304856536934389No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAC551.8189894E-1245.0000046
AATCCGA253.8886104E-545.0000042
CCCCTAG253.8886104E-545.00000432
AATTTCG253.8886104E-545.00000437
CACGGAG253.8886104E-545.0000044
CTATCAT253.8886104E-545.00000434
TATGCGT253.8886104E-545.00000441
ATCGTTC253.8886104E-545.00000441
ATCGTGC253.8886104E-545.00000429
ATCCGGC502.1827873E-1145.0000045
ATATACG502.1827873E-1145.0000041
GTATGCG253.8886104E-545.00000440
TACTGCC253.8886104E-545.00000438
TTGGTAC253.8886104E-545.00000444
CAAGCGT253.8886104E-545.00000417
GCCTAAA253.8886104E-545.00000414
TAGATTA253.8886104E-545.00000429
TATAGCC253.8886104E-545.00000434
GCAATTT253.8886104E-545.00000435
CGGTCCG253.8886104E-545.00000429