##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548787_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	605452
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.09133341701737	31.0	31.0	34.0	28.0	34.0
2	31.30043174355688	31.0	31.0	34.0	28.0	34.0
3	31.255075546864163	31.0	31.0	34.0	28.0	34.0
4	35.240161069746236	37.0	35.0	37.0	33.0	37.0
5	34.86531219650773	35.0	35.0	37.0	32.0	37.0
6	34.91660115087571	35.0	35.0	37.0	32.0	37.0
7	34.773542741621135	35.0	35.0	37.0	32.0	37.0
8	35.18164610902268	37.0	35.0	37.0	32.0	37.0
9	35.714913816454484	39.0	35.0	39.0	32.0	39.0
10	35.61049265672588	37.0	35.0	39.0	30.0	39.0
11	36.016812232844224	37.0	35.0	39.0	31.0	39.0
12	35.60023916016464	37.0	35.0	39.0	30.0	39.0
13	35.42018194671089	37.0	35.0	39.0	30.0	39.0
14	36.31944563730899	38.0	35.0	40.0	31.0	41.0
15	36.496556291828256	38.0	35.0	40.0	31.0	41.0
16	36.45447037915475	38.0	35.0	40.0	31.0	41.0
17	36.435520900087866	38.0	35.0	40.0	31.0	41.0
18	36.17338120941049	38.0	35.0	40.0	30.0	41.0
19	36.17452250550002	38.0	34.0	40.0	30.0	41.0
20	36.22344132978337	38.0	34.0	40.0	30.0	41.0
21	36.30300833096596	38.0	35.0	40.0	31.0	41.0
22	36.26263023328026	38.0	34.0	40.0	30.0	41.0
23	36.21828319998943	38.0	35.0	40.0	31.0	41.0
24	35.79827302577248	38.0	34.0	40.0	29.0	41.0
25	35.86729914179819	38.0	34.0	40.0	30.0	41.0
26	35.7557312553266	38.0	34.0	40.0	30.0	41.0
27	35.676568910499924	38.0	34.0	40.0	29.0	41.0
28	35.51168878788079	37.0	34.0	40.0	29.0	41.0
29	35.390452091990774	37.0	34.0	40.0	28.0	41.0
30	35.387601329254835	37.0	34.0	40.0	28.0	41.0
31	35.34164888380912	37.0	34.0	40.0	28.0	41.0
32	35.25323064421292	37.0	34.0	40.0	27.0	41.0
33	35.11559132681038	37.0	34.0	40.0	27.0	41.0
34	35.049908167782085	37.0	34.0	40.0	27.0	41.0
35	34.93365122255769	37.0	34.0	40.0	26.0	41.0
36	34.960287520728315	37.0	34.0	40.0	26.0	41.0
37	34.71020989277432	37.0	33.0	40.0	25.0	41.0
38	34.65845186736521	37.0	33.0	40.0	25.0	41.0
39	34.422750275826985	37.0	33.0	40.0	24.0	41.0
40	34.15611642211108	36.0	33.0	40.0	23.0	41.0
41	34.21734175458996	36.0	33.0	40.0	24.0	41.0
42	34.151582619266264	36.0	33.0	40.0	23.0	41.0
43	34.139563499666366	36.0	33.0	40.0	24.0	41.0
44	34.03679730185052	36.0	33.0	40.0	23.0	41.0
45	33.68362644767876	36.0	33.0	39.0	23.0	41.0
46	33.52753480044661	35.0	32.0	39.0	22.0	41.0
47	33.26799647205724	35.0	32.0	39.0	22.0	40.0
48	33.1992048915521	35.0	32.0	39.0	21.0	40.0
49	33.08008892529878	35.0	32.0	39.0	20.0	40.0
50	32.88344245291121	35.0	32.0	39.0	20.0	40.0
51	30.93208875352629	34.0	28.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	5.0
11	6.0
12	6.0
13	15.0
14	26.0
15	65.0
16	134.0
17	269.0
18	589.0
19	1182.0
20	1827.0
21	2765.0
22	3783.0
23	4868.0
24	6066.0
25	6749.0
26	8130.0
27	9757.0
28	11952.0
29	15187.0
30	19232.0
31	24763.0
32	31609.0
33	42029.0
34	64834.0
35	69078.0
36	60755.0
37	75756.0
38	87366.0
39	56642.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.07525617224817	40.20384770386422	18.104160197670502	23.616735926217107
2	19.44051716733944	41.14727509364904	18.519717500313813	20.8924902386977
3	18.928998500293996	31.532144579586824	26.690307406697805	22.84854951342138
4	24.934759485475315	29.879825320586935	18.037102858690698	27.148312335247056
5	15.760786982287614	39.8859034242186	19.121581892536486	25.2317277009573
6	19.891750295646887	35.42989369925279	25.02725236682677	19.651103638273554
7	71.1750890243983	14.004082900048228	8.636687962051493	6.184140113501979
8	73.89289324339502	6.587144810819025	5.896751517874249	13.623210427911708
9	69.86334176780323	12.04505063985254	11.173140067255538	6.918467525088694
10	41.393702556106845	27.90840562092453	14.294114149428857	16.403777673539768
11	32.33617198390624	23.723763403209503	24.572055257889975	19.368009354994285
12	29.947708488864517	22.90751372528293	30.874454126834166	16.270323659018388
13	23.6928773874725	34.17116468357524	22.24800644807516	19.8879514808771
14	14.968651519856241	33.49332399595674	34.27290685306185	17.265117631125175
15	13.837430547756057	29.37408745862595	32.95620462068008	23.832277372937906
16	14.760707702675028	25.15079642977478	37.09047125123048	22.99802461631971
17	14.998546540435905	26.246176410351275	28.90022660755931	29.855050441653507
18	18.407735047534736	27.670401617304098	28.09173972503188	25.83012361012929
19	20.804456835554262	30.526779992468438	29.06060265718835	19.60816051478895
20	23.5937778717388	31.618030826556026	24.394667124726652	20.393524176978524
21	18.25578245674306	29.303396470735915	25.8383819031071	26.602439169413927
22	18.081697640770862	31.24690314013332	25.576594015710576	25.09480520338524
23	21.402357247147584	29.31264575887106	20.267007128558497	29.017989865422855
24	16.221764896308873	35.27992309877579	26.02006434861888	22.478247656296453
25	18.01761328726307	27.951183578549582	27.292667296499147	26.738535837688204
26	21.641021914206245	33.31312804318096	21.224803948124705	23.821046094488086
27	16.392381229230395	30.231628601441567	27.512337889708842	25.863652279619192
28	19.7500379881477	30.732741819334976	26.316702232381754	23.20051796013557
29	18.739883591102185	28.82177282427013	27.416706857025826	25.02163672760186
30	22.05096357762465	26.76925668756565	25.890574314726848	25.28920542008285
31	25.480467485448887	29.288531543375857	18.571909911933563	26.65909105924169
32	26.83713985584324	24.16541691166269	22.112900774958213	26.884542457535858
33	23.690895397157828	27.663629817062297	21.50046576772395	27.145009018055934
34	19.073188295686528	25.16764334744951	25.8383819031071	29.920786453756865
35	18.826925999088285	26.046325720288316	30.774528781802683	24.352219498820716
36	22.882738846349504	22.518217794309045	28.8075685603483	25.791474798993146
37	22.124132053408033	25.632254910381004	27.13014409069588	25.113468945515088
38	22.666041238611815	25.250556608946706	25.56701439585632	26.516387756585164
39	23.250067718002416	22.553067790675396	29.309672773399047	24.887191717923137
40	21.235374563136304	24.76513414771113	26.366747487827276	27.632743801325294
41	18.626910143165766	20.36263816124152	34.762623626645876	26.247828068946838
42	23.74688662354737	23.528537357214113	26.58245410040763	26.14212191883089
43	21.227116270158493	22.856972972258742	28.299353210493976	27.61655754708879
44	20.666873674543975	22.82327913690928	26.89313108223278	29.616716106313962
45	25.249895945508477	27.09430309917219	24.681395056916156	22.974405898403177
46	19.769527559575327	23.386329552136257	34.684830506794924	22.159312381493496
47	22.729795260400497	24.700884628343783	24.892146693709822	27.677173417545898
48	21.670421437207242	19.5947820801649	34.69672244868296	24.038074033944888
49	22.813534351195468	19.77316120848556	31.10601666193191	26.307287778387057
50	19.719977801708477	23.58733640321611	25.68857646848966	31.004109326585755
51	19.421523093490485	22.765471086064625	32.13153148391615	25.681474336528744
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4866.0
1	3967.5
2	3069.0
3	2173.0
4	1277.0
5	1233.5
6	1190.0
7	1305.0
8	1420.0
9	1521.0
10	1622.0
11	1786.0
12	1950.0
13	1963.5
14	1977.0
15	1970.0
16	1963.0
17	2018.5
18	2074.0
19	2026.0
20	1978.0
21	1876.5
22	1775.0
23	2112.0
24	2449.0
25	2331.5
26	2516.5
27	2819.0
28	3795.5
29	4772.0
30	5732.0
31	6692.0
32	6737.5
33	6783.0
34	8442.0
35	10101.0
36	10765.5
37	11430.0
38	13266.0
39	15102.0
40	17928.5
41	20755.0
42	29149.5
43	37544.0
44	59479.5
45	81415.0
46	79785.5
47	78156.0
48	69930.5
49	61705.0
50	55428.5
51	49152.0
52	44249.0
53	39346.0
54	35172.5
55	30999.0
56	27558.0
57	24117.0
58	22799.0
59	21481.0
60	20408.5
61	19336.0
62	17430.5
63	15525.0
64	13760.5
65	11996.0
66	10092.0
67	8188.0
68	7002.5
69	5817.0
70	4704.0
71	3591.0
72	2952.0
73	2313.0
74	2658.0
75	2334.5
76	1666.0
77	1253.0
78	840.0
79	650.0
80	460.0
81	358.5
82	257.0
83	199.5
84	142.0
85	98.5
86	55.0
87	45.0
88	35.0
89	26.0
90	17.0
91	12.0
92	7.0
93	4.0
94	1.0
95	1.0
96	1.0
97	3.0
98	5.0
99	4.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	605452.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.63018267602347
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.41643370302361	21.942905706928496
2	9.135602664555615	5.048367409544769
3	3.3808332689821516	2.802391224574633
4	1.6690282983420492	1.844622270985737
5	0.975984967727279	1.3483321473678798
6	0.6466524179329403	1.0720274661227647
7	0.384896751725553	0.7444337293109557
8	0.27297283674868517	0.6033831475966803
9	0.23230168573472376	0.5776684211518752
>10	2.425140701171385	16.17973665786065
>50	1.0050948214004134	20.092417748556166
>100	0.44423748386058937	16.934468350744325
>500	0.0036067995983268424	0.6741764374753202
>1k	0.004809066131102457	2.840377249880623
>5k	6.011332663878071E-4	0.91551133859669
>10k+	0.0018033997991634212	6.379180693302445
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCC	14306	2.3628627868105148	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGT	12077	1.9947080858598205	No Hit
AATCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCT	12024	1.9859542953033438	No Hit
CTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTGCT	5512	0.9103942178735885	TruSeq Adapter, Index 16 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4199	0.6935314442763423	No Hit
AATGACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTT	2859	0.47220919247107945	No Hit
AATGATCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCT	2373	0.3919385847267826	No Hit
AAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCT	1930	0.31877010894340096	No Hit
AACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTCTG	1769	0.2921784055548582	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTAT	1485	0.24527130144090695	No Hit
AAAAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTT	1428	0.2358568474462055	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1058	0.17474547941042395	No Hit
AATGCTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCTTC	874	0.14435496125208935	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	824	0.13609666827428105	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCACTCGCTTTCGTATGCCGTCT	745	0.12304856536934389	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.261206503570886	0.0
2	0.0	0.0	0.0	1.5352166645745657	0.0
3	0.0	0.0	0.0	2.2550094805203384	0.0
4	0.0	0.0	0.0	6.282413799937897	0.0
5	0.0	0.0	0.0	6.7699834173477	0.0
6	0.0	0.0	0.0	8.783024913618256	0.0
7	0.0	0.0	0.0	10.390584224678422	0.0
8	0.0	0.0	0.0	11.287434842068405	0.0
9	0.0	0.0	0.0	14.70884562277439	0.0
10	0.0	0.0	0.0	16.48124046167161	0.0
11	0.0	0.0	0.0	20.731123193911326	0.0
12	0.0	0.0	0.0	21.685451530426853	0.0
13	0.0	0.0	0.0	22.22124957882706	0.0
14	0.0	0.0	0.0	22.89760377370956	0.0
15	0.0	0.0	0.0	23.23008264899612	0.0
16	0.0	0.0	0.0	23.78470960538573	0.0
17	0.0	0.0	0.0	24.380958358383488	0.0
18	0.0	0.0	0.0	25.394085740901012	0.0
19	0.0	0.0	0.0	25.714507508439976	0.0
20	0.0	0.0	0.0	26.07027476992396	0.0
21	0.0	0.0	0.0	26.398624498721617	0.0
22	0.0	0.0	0.0	26.725322568923712	0.0
23	0.0	0.0	0.0	27.051855473266254	0.0
24	0.0	0.0	0.0	27.269874407880394	0.0
25	0.0	0.0	0.0	27.457337658476643	0.0
26	1.651658595561663E-4	0.0	0.0	27.648930055561795	0.0
27	1.651658595561663E-4	0.0	0.0	27.87933642964265	0.0
28	1.651658595561663E-4	0.0	0.0	28.073406314621142	0.0
29	1.651658595561663E-4	0.0	0.0	28.287130936886822	0.0
30	1.651658595561663E-4	0.0	0.0	28.66205743807932	0.0
31	1.651658595561663E-4	0.0	0.0	28.86884509424364	0.0
32	1.651658595561663E-4	0.0	0.0	29.06175881820524	0.0
33	1.651658595561663E-4	0.0	0.0	29.257150030060185	0.0
34	1.651658595561663E-4	0.0	0.0	29.437841480414633	0.0
35	1.651658595561663E-4	0.0	0.0	29.6659355324617	0.0
36	1.651658595561663E-4	0.0	0.0	29.8588492564233	0.0
37	1.651658595561663E-4	0.0	0.0	30.04548667772177	0.0
38	1.651658595561663E-4	0.0	0.0	30.255412485217654	0.0
39	1.651658595561663E-4	0.0	0.0	30.458731658331295	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAC	55	1.8189894E-12	45.000004	6
AATCCGA	25	3.8886104E-5	45.000004	2
CCCCTAG	25	3.8886104E-5	45.000004	32
AATTTCG	25	3.8886104E-5	45.000004	37
CACGGAG	25	3.8886104E-5	45.000004	4
CTATCAT	25	3.8886104E-5	45.000004	34
TATGCGT	25	3.8886104E-5	45.000004	41
ATCGTTC	25	3.8886104E-5	45.000004	41
ATCGTGC	25	3.8886104E-5	45.000004	29
ATCCGGC	50	2.1827873E-11	45.000004	5
ATATACG	50	2.1827873E-11	45.000004	1
GTATGCG	25	3.8886104E-5	45.000004	40
TACTGCC	25	3.8886104E-5	45.000004	38
TTGGTAC	25	3.8886104E-5	45.000004	44
CAAGCGT	25	3.8886104E-5	45.000004	17
GCCTAAA	25	3.8886104E-5	45.000004	14
TAGATTA	25	3.8886104E-5	45.000004	29
TATAGCC	25	3.8886104E-5	45.000004	34
GCAATTT	25	3.8886104E-5	45.000004	35
CGGTCCG	25	3.8886104E-5	45.000004	29
>>END_MODULE