##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548785_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	301517
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.025865871576062	31.0	31.0	34.0	28.0	34.0
2	31.19381991728493	31.0	31.0	34.0	28.0	34.0
3	31.20622054477857	31.0	31.0	34.0	28.0	34.0
4	35.236855633347375	37.0	35.0	37.0	33.0	37.0
5	34.87403363657771	35.0	35.0	37.0	32.0	37.0
6	34.92154671212568	35.0	35.0	37.0	32.0	37.0
7	34.82283254343868	35.0	35.0	37.0	32.0	37.0
8	35.208648268588504	37.0	35.0	37.0	32.0	37.0
9	35.587880617013305	39.0	35.0	39.0	31.0	39.0
10	35.39663435229191	37.0	35.0	39.0	29.0	39.0
11	35.9080018705413	37.0	35.0	39.0	31.0	39.0
12	35.68543730535924	37.0	35.0	39.0	31.0	39.0
13	35.382416248503404	37.0	35.0	39.0	30.0	39.0
14	36.31724579376951	38.0	35.0	40.0	31.0	41.0
15	36.47089882162531	38.0	35.0	40.0	31.0	41.0
16	36.46137365389016	38.0	35.0	40.0	31.0	41.0
17	36.417883568754	38.0	35.0	40.0	31.0	41.0
18	36.203902267533834	38.0	35.0	40.0	30.0	41.0
19	36.202578959063665	38.0	34.0	40.0	30.0	41.0
20	36.167755051953954	38.0	34.0	40.0	30.0	41.0
21	36.15765943545472	38.0	34.0	40.0	30.0	41.0
22	36.084346819582315	38.0	34.0	40.0	30.0	41.0
23	36.10409694975739	38.0	34.0	40.0	30.0	41.0
24	35.74971228819602	38.0	34.0	40.0	29.0	41.0
25	35.83306082244119	38.0	34.0	40.0	30.0	41.0
26	35.684780625968024	38.0	34.0	40.0	29.0	41.0
27	35.57651475704521	37.0	34.0	40.0	29.0	41.0
28	35.26024734923735	37.0	34.0	40.0	27.0	41.0
29	34.6918847030184	37.0	33.0	40.0	25.0	41.0
30	34.76445772543505	37.0	33.0	40.0	25.0	41.0
31	34.56812385371306	37.0	33.0	40.0	25.0	41.0
32	34.49328230249041	37.0	33.0	40.0	25.0	41.0
33	34.4746896526564	37.0	33.0	40.0	25.0	41.0
34	34.37239691294355	37.0	33.0	40.0	24.0	41.0
35	34.156511904801384	37.0	33.0	40.0	23.0	41.0
36	34.06386372907664	36.0	33.0	40.0	23.0	41.0
37	33.89805218279567	36.0	33.0	40.0	23.0	41.0
38	33.91094366155142	36.0	33.0	40.0	23.0	41.0
39	33.72007548496436	36.0	33.0	40.0	22.0	41.0
40	33.5835989347201	36.0	32.0	39.0	22.0	41.0
41	33.49551103254543	36.0	32.0	39.0	21.0	41.0
42	33.417721057187485	36.0	32.0	39.0	21.0	41.0
43	33.21090353114418	35.0	32.0	39.0	21.0	41.0
44	33.21969573854874	35.0	32.0	39.0	21.0	41.0
45	33.07929569477011	35.0	31.0	39.0	20.0	40.0
46	32.79253574425323	35.0	31.0	39.0	20.0	40.0
47	32.67273818723322	35.0	31.0	39.0	20.0	40.0
48	32.58664353917026	35.0	31.0	39.0	19.0	40.0
49	32.221523164531355	35.0	31.0	39.0	17.0	40.0
50	32.02812445069432	35.0	31.0	39.0	15.0	40.0
51	30.1517692203093	34.0	26.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	6.0
10	7.0
11	10.0
12	6.0
13	9.0
14	11.0
15	29.0
16	56.0
17	140.0
18	347.0
19	661.0
20	1189.0
21	1819.0
22	2632.0
23	3285.0
24	3901.0
25	4417.0
26	4896.0
27	5804.0
28	6894.0
29	8316.0
30	10240.0
31	12753.0
32	16039.0
33	20919.0
34	31498.0
35	33663.0
36	29652.0
37	36986.0
38	40492.0
39	24837.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.169675341688862	34.48462275758912	19.406202635340627	25.93949926538139
2	20.586898914489066	36.28717452083962	20.756043606164827	22.369882958506484
3	20.521894287884265	33.52613617142649	21.72580650510585	24.2261630355834
4	20.880083046727048	33.16695244380914	18.31372692087013	27.63923758859368
5	16.60271228487946	38.698647174122854	18.375414984893055	26.323225556104628
6	19.550473107652305	38.2094541932959	20.965982017597682	21.274090681454112
7	74.83093822238878	11.085610429926007	7.870203006795637	6.213248340889568
8	76.73132858180468	8.366360769044531	6.354865563135744	8.54744508601505
9	69.46672990245989	13.925914625046017	11.169187806989324	5.438167665504764
10	38.90294742916651	32.431670519406865	12.883850661820063	15.781531389606556
11	31.043357422632887	28.09426997482729	24.54388973092728	16.318482871612545
12	29.741606609245913	23.29321398130122	31.356772586620323	15.608406822832544
13	26.1749752086947	33.52779445271742	22.877317033533764	17.41991330505411
14	14.968973557046535	37.773657870037184	30.033795772709333	17.223572800206956
15	13.202903982196693	28.87532046285947	37.977294812564466	19.94448074237937
16	14.798170584079836	29.293539004434244	33.64387414308314	22.264416268402776
17	15.281062096001222	26.075478331238372	27.725136559464307	30.9183230132961
18	22.236225486456817	26.767644942076235	26.325878806170135	24.670250765296817
19	23.872285808097054	31.579313935864313	25.17370496522584	19.37469529081279
20	24.216213347837765	28.943641652046153	23.545604393782106	23.29454060633397
21	20.971288517728684	31.692077063648156	23.98902881097915	23.347605607644013
22	16.816630571410567	32.58920724204605	24.718340922734043	25.87582126380934
23	18.714036024502764	30.828775823585403	21.048564425886436	29.408623726025397
24	16.40968834261418	30.87056451211706	26.174311896178327	26.54543524909043
25	17.498847494502797	28.346660387308177	26.831322943648285	27.32316917454074
26	19.086817658705808	33.5556535784052	22.077693795043064	25.279834967845925
27	16.805354258632182	30.742545196456582	25.916614983566433	26.535485561344803
28	22.56489683832089	28.00604941014935	29.32968953657671	20.099364214953052
29	18.62548380356663	31.601203248904707	27.077080230965418	22.696232716563244
30	22.865045751980816	28.14634000736277	26.68373590875473	22.304878331901683
31	25.333231625414154	33.09100316068414	20.58092910184169	20.994836112060018
32	23.848738213765724	30.95049367034031	21.459486529781074	23.74128158611289
33	24.321680037941476	26.76001684813792	25.94845398435246	22.96984912956815
34	23.772788930640726	27.942703064835484	23.246450448896745	25.03805755562705
35	20.578275851776183	30.255673809436946	25.43405512790324	23.731995210883632
36	24.15485694007303	25.914956702275493	30.118699774805403	19.811486582846076
37	20.31328250148416	31.650951687632872	24.98698249186613	23.048783319016838
38	22.260104737046333	27.108587575493253	25.122961557723112	25.508346129737298
39	23.46070039168604	25.416145689961095	24.967414772633052	26.15573914571981
40	26.106654019508017	26.109307269573524	21.4425720606135	26.341466650304955
41	21.445225310679	23.321736419505367	27.298626611434845	27.934411658380785
42	27.832261530858954	26.078131581303875	22.616303558339997	23.473303329497174
43	20.220418749191587	27.20841610920777	26.54311365528312	26.028051486317523
44	20.529522381822584	24.59662307597913	26.218422178517297	28.655432363680987
45	22.72840337360746	26.748077222843158	25.314990531213827	25.208528872335556
46	19.857918458992362	26.057237237038045	29.665657326120915	24.41918697784868
47	21.28967852558894	25.708334853424518	24.603919513659264	28.39806710732728
48	23.35855026416421	20.72354129286243	31.504027965255695	24.413880477717676
49	23.485242954791936	20.658536666257625	29.64841120069515	26.207809178255285
50	20.093062746047487	23.33367604480013	27.536092492297282	29.037168716855106
51	21.152041178441017	23.87328077687162	29.951545020678765	25.023133024008597
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3381.0
1	2677.5
2	1974.0
3	1394.0
4	814.0
5	805.5
6	797.0
7	797.5
8	798.0
9	863.0
10	928.0
11	975.5
12	1023.0
13	1071.0
14	1119.0
15	1131.0
16	1143.0
17	1087.0
18	1031.0
19	1101.0
20	1171.0
21	1076.5
22	982.0
23	1134.5
24	1287.0
25	1242.5
26	1622.5
27	2047.0
28	2570.5
29	3094.0
30	3340.0
31	3586.0
32	3750.0
33	3914.0
34	4063.5
35	4213.0
36	5125.5
37	6038.0
38	6802.5
39	7567.0
40	9508.0
41	11449.0
42	14693.0
43	17937.0
44	24054.0
45	30171.0
46	31564.5
47	32958.0
48	33133.5
49	33309.0
50	30739.5
51	28170.0
52	25170.0
53	22170.0
54	19393.5
55	16617.0
56	14907.0
57	13197.0
58	11990.0
59	10783.0
60	10416.0
61	10049.0
62	8997.5
63	7946.0
64	6928.0
65	5910.0
66	4974.5
67	4039.0
68	3252.0
69	2465.0
70	2146.5
71	1828.0
72	1622.0
73	1416.0
74	1334.0
75	953.0
76	654.0
77	542.5
78	431.0
79	321.0
80	211.0
81	199.5
82	188.0
83	179.5
84	171.0
85	120.0
86	69.0
87	41.5
88	14.0
89	8.5
90	3.0
91	3.5
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	301517.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.41569307237206
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.36726672419094	27.754310652246623
2	10.006768415407759	7.087932776927762
3	3.2975997223618445	3.503603389281192
4	1.4862179973820515	2.105417617356728
5	0.8330235220034404	1.475105268867011
6	0.5808121141755898	1.234191814101492
7	0.4054578496026436	1.0051699528717852
8	0.3128080868523771	0.8862652155615751
9	0.26897475872703747	0.8573334749362875
>10	3.8294024675901968	33.033437018477926
>50	0.5598658111381704	12.31208118922343
>100	0.04084430294780246	2.8299052416758212
>500	0.004981012554610057	1.1300569117426325
>1k	0.005977215065532068	4.78518947672973
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTGCT	3108	1.0307876504475701	Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2939	0.9747377428138381	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCC	2217	0.7352819244022725	No Hit
AATCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCT	2185	0.7246689241402641	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGT	1826	0.6056043274508568	No Hit
AAACTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCT	1288	0.42717326054583993	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTT	911	0.30213885120905287	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	691	0.22917447440774483	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCT	554	0.18373756703602118	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	544	0.18042100445414355	No Hit
AATGCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTC	503	0.1668230978684452	No Hit
AATGACTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTT	477	0.15820003515556336	No Hit
AACTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTG	460	0.1525618787663714	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCT	416	0.13796900340610976	No Hit
CCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTGC	355	0.11773797165665618	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCT	339	0.11243147152565196	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	334	0.11077319023471313	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	302	0.10016018997270469	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.3165625818776386E-4	0.0	0.0	1.37935837780291	0.0
2	3.3165625818776386E-4	0.0	0.0	1.752803324522332	0.0
3	3.3165625818776386E-4	0.0	0.0	2.293403025368387	0.0
4	3.3165625818776386E-4	0.0	0.0	5.193073690704007	0.0
5	3.3165625818776386E-4	0.0	0.0	5.7665073611106505	0.0
6	3.3165625818776386E-4	0.0	0.0	7.820454568067472	0.0
7	3.3165625818776386E-4	0.0	0.0	9.574252861364368	0.0
8	3.3165625818776386E-4	0.0	0.0	10.718135295853966	0.0
9	3.3165625818776386E-4	0.0	0.0	12.771087534036223	0.0
10	3.3165625818776386E-4	0.0	0.0	15.049234371527973	0.0
11	3.3165625818776386E-4	0.0	0.0	17.883237097742416	0.0
12	3.3165625818776386E-4	0.0	0.0	19.15248559782699	0.0
13	3.3165625818776386E-4	0.0	0.0	19.84034067730841	0.0
14	3.3165625818776386E-4	0.0	0.0	20.49602509974562	0.0
15	3.3165625818776386E-4	0.0	0.0	20.90495726609113	0.0
16	3.3165625818776386E-4	0.0	0.0	21.63924422171884	0.0
17	3.3165625818776386E-4	0.0	0.0	22.536706056374932	0.0
18	3.3165625818776386E-4	0.0	0.0	23.965481216647817	0.0
19	3.3165625818776386E-4	0.0	0.0	24.431789915659813	0.0
20	6.633125163755277E-4	0.0	0.0	25.01086174245565	0.0
21	6.633125163755277E-4	0.0	0.0	25.486125160438714	0.0
22	6.633125163755277E-4	0.0	0.0	25.96172023467997	0.0
23	6.633125163755277E-4	0.0	0.0	26.43598868388847	0.0
24	6.633125163755277E-4	0.0	0.0	26.739785816388462	0.0
25	6.633125163755277E-4	0.0	0.0	27.040266386306577	0.0
26	6.633125163755277E-4	0.0	0.0	27.282043798525457	0.0
27	6.633125163755277E-4	0.0	0.0	27.610051837873154	0.0
28	6.633125163755277E-4	0.0	0.0	27.875045188165178	0.0
29	6.633125163755277E-4	0.0	0.0	28.123124069289627	0.0
30	6.633125163755277E-4	0.0	0.0	28.59440761217444	0.0
31	6.633125163755277E-4	0.0	0.0	28.912134307518315	0.0
32	6.633125163755277E-4	0.0	0.0	29.18740900181416	0.0
33	6.633125163755277E-4	0.0	0.0	29.50911557225629	0.0
34	6.633125163755277E-4	0.0	0.0	29.860339549677132	0.0
35	6.633125163755277E-4	0.0	0.0	30.21786499600354	0.0
36	6.633125163755277E-4	0.0	0.0	30.496456252881263	0.0
37	6.633125163755277E-4	0.0	0.0	30.77405254098442	0.0
38	6.633125163755277E-4	0.0	0.0	31.018151547010618	0.0
39	6.633125163755277E-4	0.0	0.0	31.352461055263884	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGGT	35	1.2079363E-7	45.000004	4
CGGCACG	35	1.2079363E-7	45.000004	1
GTAGCGG	35	1.2079363E-7	45.000004	2
ACTAATA	35	1.2079363E-7	45.000004	27
TTAACGG	35	1.2079363E-7	45.000004	2
CATAACG	35	1.2079363E-7	45.000004	1
GCGAGAC	20	7.025E-4	45.0	21
CTTAACG	25	3.8839382E-5	45.0	1
TGTTTCG	25	3.8839382E-5	45.0	16
CGGGTGC	25	3.8839382E-5	45.0	6
CGGGTAT	20	7.025E-4	45.0	6
ATGGGAC	50	2.1827873E-11	45.0	5
CGGAACG	20	7.025E-4	45.0	1
GCCCAAA	20	7.025E-4	45.0	19
GATGTCA	20	7.025E-4	45.0	9
TCACCCT	20	7.025E-4	45.0	14
ATTAGCC	20	7.025E-4	45.0	31
GTTTCGT	25	3.8839382E-5	45.0	17
TACCACG	20	7.025E-4	45.0	1
CGGCCTA	20	7.025E-4	45.0	45
>>END_MODULE