SRR3548784_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548784_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	326087
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3588	1.1003198532906862	No Hit
CTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGCT	3331	1.021506530465796	Illumina PCR Primer Index 4 (95% over 23bp)
AATCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCT	1535	0.47073327056889724	RNA PCR Primer, Index 13 (95% over 23bp)
AAACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCT	1511	0.46337327155023045	TruSeq Adapter, Index 13 (95% over 23bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCC	1507	0.46214660504711935	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGT	1191	0.36523995130133985	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTT	1143	0.35051995326400626	RNA PCR Primer, Index 13 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	868	0.2661866311751159	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	626	0.1919733077368923	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCT	615	0.18859997485333668	No Hit
CCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	426	0.13063998258133563	TruSeq Adapter, Index 13 (96% over 25bp)
GAAAAACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCT	416	0.12757331632355784	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	402	0.12327998356266887	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	399	0.12235998368533552	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	333	0.10211998638400181	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGA	30	2.1607011E-6	45.000004	42
TGATCCA	30	2.1607011E-6	45.000004	33
TTGATCC	65	0.0	45.000004	17
ACAAATA	30	2.1607011E-6	45.000004	33
TGCTTGT	60	0.0	45.000004	37
CGTGCTT	30	2.1607011E-6	45.000004	12
TAGACTG	30	2.1607011E-6	45.000004	24
ACTTGCA	30	2.1607011E-6	45.000004	37
CCCTACA	30	2.1607011E-6	45.000004	27
AACCCAG	30	2.1607011E-6	45.000004	1
CTGAGAT	30	2.1607011E-6	45.000004	28
ATAGCGG	65	0.0	45.000004	2
GTGATAG	60	0.0	45.000004	1
CGATTAA	60	0.0	45.000004	24
CAACAGA	30	2.1607011E-6	45.000004	37
TCAGGCC	30	2.1607011E-6	45.000004	26
TAGCATA	65	0.0	45.000004	30
GTAGCAT	65	0.0	45.000004	29
TGCGCAT	30	2.1607011E-6	45.000004	9
TCTACGG	30	2.1607011E-6	45.000004	2