##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548784_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	326087
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.196754240432767	31.0	31.0	34.0	28.0	34.0
2	31.345524353930085	31.0	31.0	34.0	28.0	34.0
3	31.575876989883067	31.0	31.0	34.0	30.0	34.0
4	35.437064341724756	37.0	35.0	37.0	33.0	37.0
5	34.99253266766231	35.0	35.0	37.0	32.0	37.0
6	34.99210333438622	35.0	35.0	37.0	32.0	37.0
7	34.93333374222217	36.0	35.0	37.0	32.0	37.0
8	35.254376899416414	37.0	35.0	37.0	33.0	37.0
9	36.032506662332445	39.0	35.0	39.0	32.0	39.0
10	35.74041590127788	37.0	35.0	39.0	30.0	39.0
11	36.06981265735831	37.0	35.0	39.0	31.0	39.0
12	35.60322245290367	37.0	35.0	39.0	31.0	39.0
13	35.13687144841714	37.0	34.0	39.0	30.0	39.0
14	36.06013119198251	38.0	34.0	40.0	30.0	41.0
15	36.27630049649327	38.0	35.0	40.0	31.0	41.0
16	36.34929942009341	38.0	35.0	40.0	31.0	41.0
17	36.2870184950642	38.0	35.0	40.0	31.0	41.0
18	36.10525105263319	37.0	35.0	40.0	30.0	41.0
19	36.08212225571703	37.0	34.0	40.0	30.0	41.0
20	36.006863199084904	37.0	34.0	40.0	30.0	41.0
21	35.98837120155051	37.0	34.0	40.0	30.0	41.0
22	35.876401083146526	37.0	34.0	40.0	30.0	41.0
23	35.859558952058805	37.0	34.0	40.0	30.0	41.0
24	35.54970912670545	37.0	34.0	40.0	29.0	41.0
25	35.58143378914216	37.0	34.0	40.0	29.0	41.0
26	35.54479632736049	37.0	34.0	40.0	29.0	41.0
27	35.43252260899698	37.0	34.0	40.0	29.0	41.0
28	35.07090132387982	37.0	34.0	40.0	27.0	41.0
29	34.66945937740542	36.0	33.0	40.0	25.0	41.0
30	34.67322217690371	36.0	33.0	40.0	25.0	41.0
31	34.287656361645816	36.0	33.0	40.0	24.0	41.0
32	34.30720635903915	36.0	33.0	40.0	24.0	41.0
33	34.27194889707348	36.0	33.0	40.0	24.0	41.0
34	34.07709292305427	36.0	33.0	40.0	24.0	41.0
35	34.06295252493966	36.0	33.0	40.0	24.0	41.0
36	33.78351176219843	35.0	33.0	39.0	23.0	41.0
37	33.58087872254951	35.0	33.0	39.0	22.0	41.0
38	33.319690757374566	35.0	32.0	39.0	22.0	40.0
39	33.13228984902802	35.0	32.0	39.0	20.0	40.0
40	32.99191320107824	35.0	31.0	39.0	20.0	40.0
41	33.00326906623079	35.0	32.0	39.0	20.0	40.0
42	32.86947655073646	35.0	31.0	39.0	18.0	40.0
43	32.635437781941626	35.0	31.0	39.0	18.0	40.0
44	32.46669140444114	35.0	31.0	39.0	17.0	40.0
45	32.00862653218313	35.0	30.0	39.0	15.0	40.0
46	31.83325002223333	35.0	30.0	38.0	15.0	40.0
47	31.78429989542668	35.0	30.0	38.0	15.0	40.0
48	31.765074964656673	35.0	30.0	38.0	15.0	40.0
49	31.478366202884505	35.0	30.0	38.0	13.0	40.0
50	31.136006648532447	35.0	29.0	38.0	11.0	40.0
51	29.18629384182749	34.0	24.0	37.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	2.0
11	4.0
12	8.0
13	12.0
14	22.0
15	28.0
16	84.0
17	166.0
18	423.0
19	736.0
20	1299.0
21	2110.0
22	2929.0
23	3844.0
24	4645.0
25	5153.0
26	5955.0
27	6770.0
28	7937.0
29	9944.0
30	11603.0
31	14323.0
32	17986.0
33	23357.0
34	37821.0
35	39967.0
36	29597.0
37	36066.0
38	38940.0
39	24352.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.89434414742078	35.80210189305308	20.70429057276126	24.599263386764882
2	19.922597343653685	35.946541873794416	20.593583920855476	23.53727686169642
3	19.8658640178848	34.968275337563284	20.841677221109702	24.32418342344221
4	19.744424034076797	34.31507542465661	18.484024202130104	27.456476339136486
5	15.301131293182493	38.58080818922557	19.17218411037545	26.945876407216478
6	19.673890710147905	37.923314943558005	21.037637194981706	21.36515715131238
7	74.41480341135954	10.809078558789526	8.355132219315704	6.420985810535225
8	76.30417649277646	8.535758861898818	6.786532428462342	8.37353221686237
9	69.20760410565279	13.039158261445566	12.354678352709552	5.398559280192096
10	41.22304783692696	29.472196070373858	12.643558314192225	16.66119777850696
11	34.63523538196861	26.556716459104475	23.422890210281306	15.385157948645606
12	33.77902216279705	21.959783738695503	28.747542833660955	15.513651264846498
13	24.38950341473288	36.09190185441309	21.349517153397713	18.16907757745632
14	13.860411485278467	39.38151474913137	30.491555934459207	16.266517831130955
15	11.904798412693545	27.427036343061822	40.70508790598828	19.963077338256355
16	12.355598352586886	27.21666303777826	36.47462180338376	23.953116806251092
17	13.375571549923793	24.956223339170222	27.515969664537376	34.15223544636861
18	20.55831725889103	25.367769950964007	27.996209600505384	26.07770318963957
19	23.517343531020863	31.888729081502788	24.37202341706354	20.221903970412804
20	24.059223458770205	28.309622892050278	23.398663546844862	24.23249010233465
21	18.595037520661663	32.33032902262279	23.8439434874742	25.23068996924134
22	16.327237823034956	31.97980906935879	23.83873015483598	27.854222952770275
23	19.17954410939412	30.24131596782454	19.953264006231468	30.625875916549877
24	15.105784652562045	30.26431596475787	27.53191632907782	27.09798305360226
25	16.132197849040285	27.939476274736496	26.684289775428027	29.244036100795185
26	18.197597573653656	32.07732905635613	23.84333015422265	25.88174321576757
27	15.129397982746937	30.770009230665433	24.23709010172132	29.86350268486631
28	18.273650896846547	28.44516954064406	30.21432930475609	23.0668502577533
29	17.355184352642087	29.06984945735341	30.509955932005877	23.065010257998633
30	21.80215709304572	29.63380938215875	22.077237056368393	26.486796468427137
31	23.992983467602205	33.087182255042364	19.669597377387017	23.250236899968414
32	24.200290106627985	29.32131609049119	19.716824037756794	26.761569765124033
33	23.777090163054645	29.014956131339183	21.754623766050166	25.453329939556006
34	17.71551763793098	27.927822942956944	24.837543354994217	29.519116064117856
35	20.20166397311147	26.133209848905352	24.97983666935511	28.685289508628063
36	24.702303373026215	24.294436760741768	30.685062575324988	20.31819729090703
37	19.156544112460786	32.46434233808769	22.220757037232396	26.158356512219132
38	21.276530496462602	30.526515929797878	22.755890299214627	25.441063274524893
39	23.786596828453757	26.630929782542694	25.962396538347125	23.62007685065642
40	26.313223158236916	24.524130063449327	21.477090469721272	27.685556308592492
41	18.329157556112328	20.16302397826347	29.25016943331074	32.25764903231346
42	25.43615660851245	22.256023699196838	23.177250243033303	29.130569449257404
43	20.1087439855008	24.05431679275776	27.239356368085815	28.597582853655616
44	21.07842385621015	23.453250206233307	24.38950341473288	31.078822522823664
45	21.871770417097277	29.63656938179075	23.13063691591508	25.361023285196893
46	20.90975721203237	27.269103030786262	28.680689509241397	23.140450247939967
47	21.39122381450349	23.71545017127331	25.164756644699114	29.72856936952408
48	22.18978370802884	18.900170813310556	33.235302235293034	25.674743243367566
49	22.200210373305286	19.017930797609228	30.04351599419787	28.73834283488762
50	19.62758404965546	23.475636869915085	26.86644975114003	30.030329329289422
51	20.291517294464363	24.19661010711865	28.795076160656514	26.716796437760475
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4069.0
1	3226.0
2	2383.0
3	1634.5
4	886.0
5	834.5
6	783.0
7	812.0
8	841.0
9	906.5
10	972.0
11	1074.5
12	1177.0
13	1216.5
14	1256.0
15	1248.0
16	1240.0
17	1285.5
18	1331.0
19	1274.0
20	1217.0
21	1111.0
22	1005.0
23	942.0
24	879.0
25	1019.5
26	1119.5
27	1079.0
28	1252.5
29	1426.0
30	1926.0
31	2426.0
32	2969.0
33	3512.0
34	3301.0
35	3090.0
36	3404.0
37	3718.0
38	4991.0
39	6264.0
40	8702.5
41	11141.0
42	16733.0
43	22325.0
44	27831.0
45	33337.0
46	35065.5
47	36794.0
48	37759.5
49	38725.0
50	35364.0
51	32003.0
52	28478.5
53	24954.0
54	21365.5
55	17777.0
56	15904.0
57	14031.0
58	13115.5
59	12200.0
60	11843.0
61	11486.0
62	10186.5
63	8887.0
64	7654.5
65	6422.0
66	5330.0
67	4238.0
68	3930.0
69	3622.0
70	3031.5
71	2441.0
72	2213.0
73	1985.0
74	1585.0
75	1005.5
76	826.0
77	638.0
78	450.0
79	355.5
80	261.0
81	210.0
82	159.0
83	104.5
84	50.0
85	46.5
86	43.0
87	28.5
88	14.0
89	11.0
90	8.0
91	6.5
92	5.0
93	3.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	326087.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.1850942071102
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.0035960291953	26.217417769293235
2	9.996318566435313	6.634575467028812
3	3.3083962195271157	3.293683206583636
4	1.5793246483283598	2.0964014895355114
5	0.8864533195458553	1.4708518459667381
6	0.5687988254053499	1.1325385563582095
7	0.403681769842636	0.9377352293344597
8	0.2734225933927129	0.7258843616071651
9	0.24657597729124173	0.736438233205811
>10	2.5820833950858506	20.916524139789015
>50	0.9793423368834304	22.95987226669781
>100	0.16206375727106512	7.627005149194341
>500	0.00298276853873126	0.695853688339283
>1k	0.006959793257039606	4.555218597065975
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3588	1.1003198532906862	No Hit
CTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGCT	3331	1.021506530465796	Illumina PCR Primer Index 4 (95% over 23bp)
AATCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCT	1535	0.47073327056889724	RNA PCR Primer, Index 13 (95% over 23bp)
AAACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCT	1511	0.46337327155023045	TruSeq Adapter, Index 13 (95% over 23bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCC	1507	0.46214660504711935	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGT	1191	0.36523995130133985	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTT	1143	0.35051995326400626	RNA PCR Primer, Index 13 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	868	0.2661866311751159	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	626	0.1919733077368923	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCT	615	0.18859997485333668	No Hit
CCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	426	0.13063998258133563	TruSeq Adapter, Index 13 (96% over 25bp)
GAAAAACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCT	416	0.12757331632355784	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	402	0.12327998356266887	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	399	0.12235998368533552	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	333	0.10211998638400181	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.6575331123289183	0.0
2	0.0	0.0	0.0	2.151573046456927	0.0
3	0.0	0.0	0.0	2.713079638256048	0.0
4	0.0	0.0	0.0	5.892905880945882	0.0
5	0.0	0.0	0.0	6.46882580415656	0.0
6	0.0	0.0	0.0	8.928905476145937	0.0
7	0.0	0.0	0.0	10.918865210817971	0.0
8	0.0	0.0	0.0	12.423985010135333	0.0
9	0.0	0.0	0.0	14.524958063338925	0.0
10	0.0	0.0	0.0	17.36990435067942	0.0
11	0.0	0.0	0.0	20.241223967836806	0.0
12	0.0	0.0	0.0	21.63809044825461	0.0
13	0.0	0.0	0.0	22.4581170055844	0.0
14	0.0	0.0	0.0	23.093836920821744	0.0
15	0.0	0.0	0.0	23.49802353359686	0.0
16	0.0	0.0	0.0	24.303943426140876	0.0
17	0.0	0.0	0.0	25.166289977828004	0.0
18	0.0	0.0	0.0	26.686743108434253	0.0
19	0.0	0.0	0.0	27.127423049676928	0.0
20	0.0015333331288889163	0.0	0.0	27.690769641230716	0.0
21	0.0015333331288889163	0.0	0.0	28.138809581492055	0.0
22	0.0015333331288889163	0.0	0.0	28.558329525556065	0.0
23	0.0015333331288889163	0.0	0.0	28.974476136736516	0.0
24	0.0015333331288889163	0.0	0.0	29.267956097605854	0.0
25	0.0015333331288889163	0.0	0.0	29.50776939896408	0.0
26	0.0015333331288889163	0.0	0.0	29.751569366457417	0.0
27	0.0015333331288889163	0.0	0.0	30.059462658738312	0.0
28	0.0015333331288889163	0.0	0.0	30.27259596365387	0.0
29	0.0015333331288889163	0.0	0.0	30.499222600103653	0.0
30	0.0018399997546666993	0.0	0.0	30.967502537666327	0.0
31	0.0018399997546666993	0.0	0.0	31.212529171662776	0.0
32	0.0018399997546666993	0.0	0.0	31.47779580296056	0.0
33	0.0018399997546666993	0.0	0.0	31.734169102110787	0.0
34	0.0018399997546666993	0.0	0.0	32.02703572972857	0.0
35	0.0018399997546666993	0.0	0.0	32.340449021273464	0.0
36	0.0018399997546666993	0.0	0.0	32.5428489942868	0.0
37	0.0018399997546666993	0.0	0.0	32.74494230067436	0.0
38	0.0018399997546666993	0.0	0.0	32.954088939454806	0.0
39	0.0018399997546666993	0.0	0.0	33.2150622379917	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGA	30	2.1607011E-6	45.000004	42
TGATCCA	30	2.1607011E-6	45.000004	33
TTGATCC	65	0.0	45.000004	17
ACAAATA	30	2.1607011E-6	45.000004	33
TGCTTGT	60	0.0	45.000004	37
CGTGCTT	30	2.1607011E-6	45.000004	12
TAGACTG	30	2.1607011E-6	45.000004	24
ACTTGCA	30	2.1607011E-6	45.000004	37
CCCTACA	30	2.1607011E-6	45.000004	27
AACCCAG	30	2.1607011E-6	45.000004	1
CTGAGAT	30	2.1607011E-6	45.000004	28
ATAGCGG	65	0.0	45.000004	2
GTGATAG	60	0.0	45.000004	1
CGATTAA	60	0.0	45.000004	24
CAACAGA	30	2.1607011E-6	45.000004	37
TCAGGCC	30	2.1607011E-6	45.000004	26
TAGCATA	65	0.0	45.000004	30
GTAGCAT	65	0.0	45.000004	29
TGCGCAT	30	2.1607011E-6	45.000004	9
TCTACGG	30	2.1607011E-6	45.000004	2
>>END_MODULE