SRR3548781_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548781_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	434373
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCC	6378	1.46832330738789	No Hit
AATCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCT	5577	1.2839195806369181	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGT	5429	1.2498474813121443	No Hit
CTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTGCT	4601	1.0592278986032742	TruSeq Adapter, Index 14 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4057	0.9339899118959972	No Hit
AAACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCT	1896	0.4364912183768328	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTT	1552	0.3572966091354665	No Hit
AATGACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTT	1340	0.30849062902160124	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCT	1068	0.24587163566796277	No Hit
AACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTCTG	1010	0.2325190562028487	TruSeq Adapter, Index 15 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	985	0.22676363401960986	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCT	799	0.18394329297631298	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	719	0.16552594198994872	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTAT	656	0.15102227808818688	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCT	564	0.12984232445386798	No Hit
AATGCTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTTC	475	0.10935302148153776	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	452	0.10405803307295804	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	447	0.10290694863631027	No Hit
GAAAACTGTCTCTTATACACATCTGACGCGGATAGTGTCGTATGCCGTCTT	437	0.10060477976301474	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCAC	20	7.0284365E-4	45.0	19
TATCATA	25	3.8867845E-5	45.0	34
ATCCTCC	20	7.0284365E-4	45.0	39
CAGAATA	25	3.8867845E-5	45.0	23
CGGAAAT	20	7.0284365E-4	45.0	6
AATCCAG	20	7.0284365E-4	45.0	1
GATACAC	20	7.0284365E-4	45.0	11
TACTTTC	20	7.0284365E-4	45.0	25
TCCGCAA	20	7.0284365E-4	45.0	32
CGTATCG	20	7.0284365E-4	45.0	13
GTCGAAA	25	3.8867845E-5	45.0	36
CCGATTC	20	7.0284365E-4	45.0	39
ACGCATT	35	1.2095916E-7	45.0	12
TCCGATT	20	7.0284365E-4	45.0	38
ACGTTCA	20	7.0284365E-4	45.0	38
ATGGCAT	20	7.0284365E-4	45.0	18
ACTTGCA	20	7.0284365E-4	45.0	14
CGTGCCA	20	7.0284365E-4	45.0	36
TTAGCCC	20	7.0284365E-4	45.0	21
CTATCAG	20	7.0284365E-4	45.0	20

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content