##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548779_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	533079
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.0453947726322	31.0	31.0	34.0	28.0	34.0
2	31.24397134383459	31.0	31.0	34.0	28.0	34.0
3	31.250403786305593	31.0	31.0	34.0	28.0	34.0
4	35.25165875977107	37.0	35.0	37.0	33.0	37.0
5	34.873626610689975	35.0	35.0	37.0	32.0	37.0
6	34.94155275296907	35.0	35.0	37.0	32.0	37.0
7	34.812464944220274	35.0	35.0	37.0	32.0	37.0
8	35.210383451608486	37.0	35.0	37.0	32.0	37.0
9	35.649273372239385	39.0	35.0	39.0	31.0	39.0
10	35.58988255024115	37.0	35.0	39.0	30.0	39.0
11	35.974628525978325	37.0	35.0	39.0	31.0	39.0
12	35.47421676712082	37.0	35.0	39.0	30.0	39.0
13	35.104995694821966	37.0	34.0	39.0	30.0	39.0
14	36.030999157723336	38.0	34.0	40.0	30.0	41.0
15	36.25378414831573	38.0	35.0	40.0	31.0	41.0
16	36.27627237238758	38.0	35.0	40.0	31.0	41.0
17	36.24831591565228	38.0	35.0	40.0	31.0	41.0
18	36.056982173373925	37.0	35.0	40.0	30.0	41.0
19	36.01536545240011	37.0	34.0	40.0	30.0	41.0
20	35.97525132297464	37.0	34.0	40.0	30.0	41.0
21	35.98974636029556	37.0	34.0	40.0	30.0	41.0
22	35.959604486389445	37.0	34.0	40.0	30.0	41.0
23	35.89414514546625	37.0	34.0	40.0	30.0	41.0
24	35.523787281059654	37.0	34.0	40.0	29.0	41.0
25	35.647952742464064	37.0	34.0	40.0	30.0	41.0
26	35.51228241967888	37.0	34.0	40.0	29.0	41.0
27	35.40947026613316	37.0	34.0	40.0	29.0	41.0
28	35.318678844974194	37.0	34.0	40.0	29.0	41.0
29	35.13248880559917	37.0	34.0	40.0	27.0	41.0
30	35.16940265889296	37.0	34.0	40.0	27.0	41.0
31	34.91726554600725	36.0	34.0	40.0	27.0	41.0
32	34.85396348383635	37.0	34.0	40.0	27.0	41.0
33	34.67649823009348	36.0	33.0	40.0	26.0	41.0
34	34.71159809334076	36.0	34.0	40.0	26.0	41.0
35	34.582866704559734	36.0	33.0	40.0	25.0	41.0
36	34.38754105864234	36.0	33.0	40.0	25.0	41.0
37	34.27109115159291	36.0	33.0	40.0	24.0	41.0
38	34.0939485517156	36.0	33.0	40.0	24.0	41.0
39	34.004590314005995	36.0	33.0	39.0	24.0	41.0
40	33.733951253003774	35.0	33.0	39.0	23.0	41.0
41	33.80123959113002	35.0	33.0	39.0	23.0	41.0
42	33.84809943741922	35.0	33.0	39.0	23.0	41.0
43	33.78621367564657	35.0	33.0	39.0	23.0	40.0
44	33.75698161060556	35.0	33.0	39.0	23.0	40.0
45	33.52255481832899	35.0	33.0	39.0	23.0	40.0
46	33.34024788070811	35.0	32.0	39.0	23.0	40.0
47	33.18650143787318	35.0	32.0	39.0	22.0	40.0
48	33.12388407721932	35.0	32.0	39.0	22.0	40.0
49	32.997131757206716	35.0	32.0	39.0	20.0	40.0
50	32.85740199857807	35.0	32.0	39.0	20.0	40.0
51	30.93765089226925	34.0	28.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	6.0
10	11.0
11	8.0
12	13.0
13	24.0
14	25.0
15	75.0
16	130.0
17	306.0
18	625.0
19	1204.0
20	1897.0
21	2883.0
22	3744.0
23	5057.0
24	5609.0
25	6417.0
26	7345.0
27	8671.0
28	10439.0
29	13188.0
30	16878.0
31	21774.0
32	28823.0
33	39336.0
34	63694.0
35	65850.0
36	53154.0
37	64309.0
38	70154.0
39	41427.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.347601387411622	36.17906539180872	19.783746874290678	24.68958634648898
2	19.973212225580074	38.94394639443684	18.890633470836406	22.192207909146674
3	19.84677693174933	32.45335119184961	24.193787412372274	23.506084464028785
4	22.316579718953474	32.68314827633428	18.421659829030972	26.578612175681275
5	16.09404985002223	39.794664580671906	18.391270337041977	25.720015232263886
6	21.34186490182506	35.83915329622814	21.38163386665016	21.437347935296643
7	72.80646958518344	12.818737935653065	8.17346021884186	6.201332260321641
8	74.97575406271866	8.264441105352114	6.432630060460082	10.327174771469144
9	69.45143215170735	13.558215574051877	11.101919227731724	5.888433046509054
10	43.89949707266653	27.71765535689832	12.95042573427203	15.43242183616312
11	37.38714149309953	24.462978282768596	20.754897491741374	17.39498273239051
12	33.87809311565453	23.634208062970032	26.81628801734827	15.67141080402717
13	23.78615552291499	36.953059490244414	21.049225349338467	18.211559637502134
14	15.092697330039261	37.73962208228049	30.58964993931481	16.578030648365438
15	13.187913986482302	28.61208188654965	36.342455808613735	21.857548318354315
16	14.51192037202741	25.74121284087349	38.090039187437505	21.65682759966159
17	14.967012394035406	26.23813731173053	28.133916361364825	30.660933932869238
18	19.853717741648047	26.13927766803795	27.088855497965593	26.918149092348415
19	22.097850412415422	31.86056850860754	26.778394947090394	19.26318613188664
20	26.167040907632828	30.246924001883396	22.970891744000422	20.615143346483357
21	19.336908788378455	32.12563241095597	23.925534489259565	24.611924311406003
22	18.8626826417848	31.379026373201718	23.9714939061565	25.78679707885698
23	20.691867434282724	30.78793199507015	20.304682795608155	28.215517775038972
24	17.07401717193887	32.06841762665571	27.22748410648328	23.630081094922144
25	18.3466240463421	27.19278005698968	27.215853560166504	27.24474233650172
26	19.918811283130644	33.3517171000921	24.166774530604282	22.56269708617297
27	16.93951553146907	31.260094657639865	25.772915459059536	26.027474351831533
28	17.889093361396714	29.722236291431475	28.156427096171488	24.23224325100032
29	16.89636995642297	29.564285968871406	30.22703951947085	23.31230455523478
30	19.396937414529553	29.769133655612016	23.278350863568065	27.555578066290366
31	26.714614531804852	32.06504101643471	19.29113696093825	21.929207490822186
32	24.679644105282705	27.270442092072656	20.323629330737095	27.72628447190754
33	23.00090605707597	31.4947690679993	20.138853715865753	25.365471159058977
34	20.864637323923844	28.200323029044476	22.55819493921164	28.37684470782004
35	17.509224711534312	29.308789128815803	24.7464259518758	28.43556020777408
36	23.97336980072372	28.21683090123603	26.449363039999703	21.36043625804055
37	20.18762697461352	31.92603722900358	20.246342474567562	27.639993321815343
38	22.043074291052545	34.260025249540874	21.0038287008117	22.69307175859488
39	22.310389266881643	27.47622772609688	23.893456692160072	26.319926314861398
40	25.356654454593038	29.448168095160383	21.013583352561252	24.181594097685334
41	20.85394472489068	24.257380238201094	27.933008053215374	26.95566698369285
42	25.48909261103889	27.081164330239982	21.829785078759436	25.599957979961697
43	22.69138345348438	25.230969518589173	25.065140438846772	27.012506589079667
44	21.22555943865731	24.695214030190645	24.993856445292348	29.085370085859697
45	24.164898636037062	30.153879631349202	22.581268442388463	23.099953290225276
46	20.018233695193395	26.5238360543184	29.928397104369147	23.529533146119057
47	21.837476246485043	27.473226294789328	23.678666764213187	27.010630694512443
48	23.857814695382864	21.49306200394313	31.09858013540207	23.55054316527194
49	22.676939065316773	20.9522416002131	29.74043246873353	26.6303868657366
50	20.093832246252433	25.448760877843625	24.90944118976737	29.54796568613658
51	19.011065902052042	24.84978774252972	28.923292795251736	27.215853560166504
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5619.0
1	4582.5
2	3546.0
3	2560.0
4	1574.0
5	1465.5
6	1357.0
7	1441.0
8	1525.0
9	1656.0
10	1787.0
11	1855.0
12	1923.0
13	2006.0
14	2089.0
15	2113.0
16	2137.0
17	2019.0
18	1901.0
19	1827.0
20	1753.0
21	1659.0
22	1565.0
23	1573.5
24	1582.0
25	1971.5
26	2171.0
27	1981.0
28	2786.0
29	3591.0
30	3795.5
31	4000.0
32	5648.0
33	7296.0
34	7391.5
35	7487.0
36	8381.0
37	9275.0
38	10238.5
39	11202.0
40	15100.5
41	18999.0
42	26379.5
43	33760.0
44	48880.0
45	64000.0
46	62505.5
47	61011.0
48	59164.5
49	57318.0
50	51182.5
51	45047.0
52	41343.5
53	37640.0
54	34067.0
55	30494.0
56	26823.0
57	23152.0
58	20940.5
59	18729.0
60	17063.5
61	15398.0
62	15607.0
63	15816.0
64	13401.0
65	10986.0
66	9112.0
67	7238.0
68	6080.0
69	4922.0
70	4221.0
71	3520.0
72	3333.5
73	3147.0
74	2707.0
75	2040.0
76	1813.0
77	1457.0
78	1101.0
79	780.0
80	459.0
81	391.5
82	324.0
83	283.0
84	242.0
85	149.0
86	56.0
87	57.0
88	58.0
89	33.0
90	8.0
91	9.0
92	10.0
93	9.5
94	9.0
95	6.0
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	533079.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.056179512885567
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.5514136043766	23.114601541925737
2	9.08340812341734	5.278582740456344
3	3.497800946691749	3.0489819662224957
4	1.7592031619008508	2.0446289148730803
5	1.0203653268700927	1.4823959053130786
6	0.6606722221274867	1.1517966411187956
7	0.39937002669255844	0.8122917031351413
8	0.3162301861921755	0.7350752845914462
9	0.26338699228987633	0.6887717756400288
>10	2.0588954591955195	14.262112508573058
>50	0.8470743552504335	18.037760555368315
>100	0.5304778771176928	20.974289537835496
>500	0.00455066806350962	0.9148083019483176
>1k	0.00455066806350962	2.598221803282905
>5k	0.002600381750576926	4.855680819715766
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCC	7312	1.3716541075525392	No Hit
AATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCT	6630	1.243718098068016	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGT	6167	1.1568641796056494	No Hit
CTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGCT	5597	1.04993818927401	Illumina Single End Adapter 2 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4873	0.9141234226071557	No Hit
AAACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCT	2008	0.3766796290981262	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTT	1580	0.29639134162103553	No Hit
AATGACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTT	1457	0.2733178384442081	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCT	1414	0.26525149180515456	No Hit
AACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTG	1257	0.2357999470997732	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1166	0.21872930653805533	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	903	0.16939327942012347	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCT	872	0.16357800626173605	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCACAGATCGTAT	768	0.14406870276262992	No Hit
ACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACA	650	0.12193314686941335	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCT	596	0.11180331620641594	No Hit
ATGAATAAATTGGCCCTGAGGACATTTTCAAACTTCCTTTAAGTCACATAT	550	0.10317420119719592	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.875894567221744E-4	0.0	0.0	1.379157685821426	0.0
2	1.875894567221744E-4	0.0	0.0	1.6843657319084038	0.0
3	1.875894567221744E-4	0.0	0.0	2.2996591499571357	0.0
4	1.875894567221744E-4	0.0	0.0	5.473860347153049	0.0
5	1.875894567221744E-4	0.0	0.0	5.9176970017577135	0.0
6	1.875894567221744E-4	0.0	0.0	7.95004117588575	0.0
7	1.875894567221744E-4	0.0	0.0	9.69687419688264	0.0
8	1.875894567221744E-4	0.0	0.0	10.856927397252564	0.0
9	1.875894567221744E-4	0.0	0.0	13.384882916040587	0.0
10	1.875894567221744E-4	0.0	0.0	15.496014661991937	0.0
11	1.875894567221744E-4	0.0	0.0	18.96022915928033	0.0
12	1.875894567221744E-4	0.0	0.0	20.06513105937394	0.0
13	1.875894567221744E-4	0.0	0.0	20.594508506243915	0.0
14	1.875894567221744E-4	0.0	0.0	21.1696577805541	0.0
15	1.875894567221744E-4	0.0	0.0	21.543898746714838	0.0
16	1.875894567221744E-4	0.0	0.0	22.163881901181625	0.0
17	1.875894567221744E-4	0.0	0.0	22.805062664258017	0.0
18	1.875894567221744E-4	0.0	0.0	23.962677201690557	0.0
19	1.875894567221744E-4	0.0	0.0	24.323224137510575	0.0
20	1.875894567221744E-4	0.0	0.0	24.74023549980397	0.0
21	1.875894567221744E-4	0.0	0.0	25.12329317043065	0.0
22	1.875894567221744E-4	0.0	0.0	25.464518392208284	0.0
23	1.875894567221744E-4	0.0	0.0	25.838009000542133	0.0
24	3.751789134443488E-4	0.0	0.0	26.090129230376736	0.0
25	3.751789134443488E-4	0.0	0.0	26.314298631159733	0.0
26	3.751789134443488E-4	0.0	0.0	26.529651327476792	0.0
27	3.751789134443488E-4	0.0	0.0	26.757947696307678	0.0
28	3.751789134443488E-4	0.0	0.0	26.954541446952515	0.0
29	3.751789134443488E-4	0.0	0.0	27.18696478383129	0.0
30	3.751789134443488E-4	0.0	0.0	27.628925543868732	0.0
31	3.751789134443488E-4	0.0	0.0	27.872791837607558	0.0
32	3.751789134443488E-4	0.0	0.0	28.093021859799393	0.0
33	3.751789134443488E-4	0.0	0.0	28.310062861226946	0.0
34	3.751789134443488E-4	0.0	0.0	28.504780717304566	0.0
35	3.751789134443488E-4	0.0	0.0	28.78128757651305	0.0
36	5.627683701665231E-4	0.0	0.0	28.979382042811665	0.0
37	5.627683701665231E-4	0.0	0.0	29.182353834985058	0.0
38	5.627683701665231E-4	0.0	0.0	29.39132848977356	0.0
39	5.627683701665231E-4	0.0	0.0	29.610995743595225	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTACG	35	1.2102828E-7	45.000004	1
ACAACGC	35	1.2102828E-7	45.000004	31
GGGTCTA	35	1.2102828E-7	45.000004	7
TAGAACG	35	1.2102828E-7	45.000004	1
TATTGCG	35	1.2102828E-7	45.000004	1
CTGCCTA	35	1.2102828E-7	45.000004	20
TGTTACC	35	1.2102828E-7	45.000004	11
CATACCG	35	1.2102828E-7	45.000004	1
GCTACGT	35	1.2102828E-7	45.000004	1
CCTATGA	35	1.2102828E-7	45.000004	23
TATCGCG	35	1.2102828E-7	45.000004	1
GCGTAAG	35	1.2102828E-7	45.000004	1
CTTAACA	25	3.8879814E-5	45.0	27
AGCGTTC	20	7.0298783E-4	45.0	17
CTAGAAA	20	7.0298783E-4	45.0	26
AGGTACC	20	7.0298783E-4	45.0	41
CTCACTA	45	3.8380676E-10	45.0	44
ACTATAG	25	3.8879814E-5	45.0	1
TCGTTAA	20	7.0298783E-4	45.0	44
GTACCGT	20	7.0298783E-4	45.0	22
>>END_MODULE