SRR3548778_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548778_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	489511
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCC	8011	1.6365311504746576	No Hit
AATCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCT	7704	1.5738155015924054	TruSeq Adapter, Index 3 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGT	7614	1.5554298064803447	No Hit
CTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGCT	4666	0.9531961488097306	TruSeq Adapter, Index 3 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2746	0.5609679864190998	No Hit
AATGACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTT	1656	0.33829679006191893	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCT	1462	0.29866540282036563	No Hit
AAACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCT	1114	0.2275740483870638	RNA PCR Primer, Index 24 (95% over 21bp)
AACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTG	1088	0.2222626253546907	RNA PCR Primer, Index 3 (95% over 22bp)
AATGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTC	1055	0.21552120381360174	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTAT	900	0.18385695112060813	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	683	0.13952699735041704	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTT	683	0.13952699735041704	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	668	0.13646271483174024	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	571	0.1166470212109636	RNA PCR Primer, Index 3 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	498	0.10173417962006982	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGT	30	2.1629385E-6	45.000004	36
CTTCGGT	20	7.0293125E-4	45.000004	24
TTTAGCG	35	1.21001E-7	45.000004	1
CCGGGTA	40	6.8012014E-9	45.000004	5
GGTACGA	30	2.1629385E-6	45.000004	30
ACACGTG	60	0.0	45.000004	42
TCGCAAG	20	7.0293125E-4	45.000004	1
GCGACGC	20	7.0293125E-4	45.000004	25
TACTTTA	20	7.0293125E-4	45.000004	38
CGTATCT	20	7.0293125E-4	45.000004	42
GATCGGA	20	7.0293125E-4	45.000004	21
TTCACCG	20	7.0293125E-4	45.000004	1
CACGAGA	20	7.0293125E-4	45.000004	24
TCTAGTC	20	7.0293125E-4	45.000004	32
TCTAGCG	35	1.21001E-7	45.000004	1
TTTCAAT	20	7.0293125E-4	45.000004	45
CGTTACG	20	7.0293125E-4	45.000004	1
GTTACGG	80	0.0	45.000004	2
TCGGAAT	20	7.0293125E-4	45.000004	9
GTTCGTG	30	2.1629385E-6	45.000004	12