##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548778_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	489511
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.43508930340687	31.0	31.0	34.0	30.0	34.0
2	31.60518966887363	31.0	31.0	34.0	30.0	34.0
3	31.755560140630138	31.0	31.0	34.0	30.0	34.0
4	35.573265973594054	37.0	35.0	37.0	33.0	37.0
5	35.17814921421582	37.0	35.0	37.0	32.0	37.0
6	35.172751991272925	37.0	35.0	37.0	32.0	37.0
7	35.124293427522566	37.0	35.0	37.0	32.0	37.0
8	35.41853604924098	37.0	35.0	37.0	33.0	37.0
9	36.341892214883835	39.0	37.0	39.0	32.0	39.0
10	36.053892558083476	38.0	35.0	39.0	31.0	39.0
11	36.32223790680904	38.0	35.0	39.0	32.0	39.0
12	35.93392794033229	37.0	35.0	39.0	32.0	39.0
13	35.699775898805136	37.0	35.0	39.0	30.0	39.0
14	36.744350995176816	38.0	35.0	40.0	31.0	41.0
15	36.81788560420501	38.0	35.0	40.0	32.0	41.0
16	36.78002741511427	38.0	35.0	40.0	31.0	41.0
17	36.70574307829651	38.0	35.0	40.0	31.0	41.0
18	36.46442061567564	38.0	35.0	40.0	31.0	41.0
19	36.521299827787324	38.0	35.0	40.0	31.0	41.0
20	36.53580205552071	38.0	35.0	40.0	31.0	41.0
21	36.61907086868324	38.0	35.0	40.0	31.0	41.0
22	36.57243248874897	38.0	35.0	40.0	31.0	41.0
23	36.56067381529731	38.0	35.0	40.0	31.0	41.0
24	36.27075387478524	38.0	35.0	40.0	30.0	41.0
25	36.290479682785474	38.0	35.0	40.0	31.0	41.0
26	36.215649903679385	38.0	35.0	40.0	30.0	41.0
27	36.13553117294606	38.0	35.0	40.0	30.0	41.0
28	36.026896229093936	38.0	35.0	40.0	30.0	41.0
29	35.77119819575045	38.0	34.0	40.0	29.0	41.0
30	35.77565774824264	38.0	34.0	40.0	29.0	41.0
31	35.65806692801592	38.0	34.0	40.0	29.0	41.0
32	35.662522394798074	38.0	34.0	40.0	29.0	41.0
33	35.54589171642721	38.0	34.0	40.0	29.0	41.0
34	35.598924232550445	38.0	34.0	40.0	29.0	41.0
35	35.473153820853874	38.0	34.0	40.0	29.0	41.0
36	35.333361252351835	38.0	34.0	40.0	28.0	41.0
37	35.25565921909824	38.0	34.0	40.0	27.0	41.0
38	35.15455423882201	37.0	34.0	40.0	27.0	41.0
39	35.149698372457415	37.0	34.0	40.0	27.0	41.0
40	34.89992461865004	37.0	34.0	40.0	26.0	41.0
41	35.05240535963441	37.0	34.0	40.0	27.0	41.0
42	35.0350778634188	37.0	34.0	40.0	27.0	41.0
43	34.91472510321525	37.0	34.0	40.0	27.0	41.0
44	34.764730516781036	37.0	34.0	40.0	26.0	41.0
45	34.56010998731387	37.0	33.0	40.0	25.0	41.0
46	34.46974225298308	36.0	33.0	40.0	25.0	41.0
47	34.34314039929644	36.0	33.0	40.0	24.0	41.0
48	34.38198324450319	36.0	33.0	40.0	25.0	41.0
49	34.139151112028124	36.0	33.0	39.0	24.0	41.0
50	33.87494050184776	36.0	33.0	39.0	23.0	40.0
51	31.840481623497734	35.0	30.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	5.0
10	6.0
11	3.0
12	11.0
13	7.0
14	9.0
15	37.0
16	64.0
17	154.0
18	337.0
19	666.0
20	1109.0
21	1799.0
22	2474.0
23	3056.0
24	3830.0
25	4241.0
26	5159.0
27	6191.0
28	7719.0
29	9724.0
30	12832.0
31	16732.0
32	22645.0
33	31248.0
34	50482.0
35	54949.0
36	48568.0
37	64905.0
38	81679.0
39	58865.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.7315504656688	37.80282771990823	19.235318511739266	24.230303302683698
2	21.9339299831873	36.383452057257145	19.48005254223092	22.202565417324635
3	19.673919482912538	30.247941312861204	26.411051028475356	23.6670881757509
4	23.922445052307303	31.312473059849523	18.376502264504783	26.388579623338394
5	16.861929558273463	37.92519473515406	18.826747509249024	26.38612819732345
6	19.655125216797988	36.28886787018065	23.635219637556666	20.4207872754647
7	75.94252223136967	11.034889920757655	7.5027936042295265	5.519794243643146
8	78.10610997505674	5.507741399069683	5.55104992533365	10.835098700539927
9	72.80020265121723	10.587708958532085	10.362994907162454	6.249093483088225
10	40.981510119282305	30.218524200681905	13.081013501228778	15.718952178807013
11	33.52631503684289	24.208444754050472	25.12057951710993	17.14466069199671
12	31.450774344192467	21.86712862428015	31.035053349158648	15.64704368236873
13	24.6844299719516	33.6870877263228	22.38274522942283	19.245737072302767
14	16.315465842442762	34.534259699986315	31.533918543199235	17.616355914371688
15	14.695481817568961	27.087848894100443	35.36161598002905	22.85505330830155
16	16.140801738878185	25.409234930369283	35.202069003556616	23.247894327195915
17	16.818212461007004	24.878501198134465	28.6632986797028	29.639987661155725
18	21.360296295691008	26.36651678920392	27.449229945803054	24.823956969302017
19	22.727579155524595	29.659803354776503	28.14972492957257	19.46289256012633
20	25.627207560197828	29.713121870601476	23.55575257757231	21.10391799162838
21	20.535187156161967	28.60671159585791	26.39368676086952	24.464414487110606
22	18.821436086216654	29.90351595776193	25.969590060284652	25.305457895736765
23	20.974400983838972	27.794268157406066	22.118399790811647	29.112931067943315
24	17.860068517357117	31.525134266645694	27.70867253238436	22.90612468361283
25	19.442464010001817	26.604917969156975	27.8659723683431	26.086645652498103
26	23.008267434235393	30.92984631601741	22.246691085593582	23.815195164153614
27	17.481731769051155	28.379954689475824	29.05613969859717	25.08217384287585
28	21.51371470712609	26.041089985720443	31.381112988267883	21.064082318885582
29	20.840798266024667	27.94605228483119	29.019572593874294	22.19357685526985
30	22.548012199930135	27.96913654647189	27.56955410603643	21.913297147561547
31	29.769504668945128	27.874143788392907	21.851807211686765	20.504544330975197
32	26.119331332697328	24.613134332017054	23.885469376581938	25.382064958703687
33	26.722586417874165	27.332582924592092	26.106869917121372	19.83796074041237
34	22.547195057925155	23.989246411214456	27.62042119584647	25.84313733501392
35	24.312834645186726	24.646024297717517	29.805254631663026	21.235886425432728
36	28.580563051698533	23.95676501651648	29.580949151295886	17.881722780489103
37	23.31714711211801	28.1149963943609	25.117719520092503	23.450136973428584
38	25.858663033108552	28.38976039353559	23.989450696715704	21.762125876640155
39	23.363928491903145	24.807001272698674	27.85003809924598	23.9790321361522
40	25.828633064425517	27.78609673735626	23.51734690333823	22.867923294879994
41	20.86061395964544	21.01852665210792	31.971702372367528	26.14915701587911
42	27.658009728075566	22.306138166455913	23.827248008727075	26.208604096741446
43	23.045447395462002	23.06934879910768	28.738475744161008	25.146728061269307
44	21.38950912236906	23.165975841196623	27.780581028822642	27.66393400761168
45	26.12157847321102	27.150564542982693	24.08669059530838	22.64116638849791
46	19.840412166427313	24.226421878160043	33.119378318362614	22.813787637050037
47	22.202156846322147	26.705426435769574	25.246419385876926	25.845997332031356
48	22.167632596611718	20.242650318378956	34.17941578432354	23.410301300685788
49	23.134515874004872	21.55559323488134	30.159485690822063	25.15040520029172
50	21.303709211846108	22.864450441358823	27.25454586311646	28.57729448367861
51	20.14316327927258	23.50733691377722	31.150678942863387	25.198820864086812
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3139.0
1	2524.0
2	1909.0
3	1354.0
4	799.0
5	728.5
6	658.0
7	693.5
8	729.0
9	796.0
10	863.0
11	919.5
12	976.0
13	1033.0
14	1090.0
15	1063.5
16	1037.0
17	1066.0
18	1095.0
19	1091.0
20	1087.0
21	1012.0
22	937.0
23	1469.5
24	2002.0
25	2355.5
26	2764.0
27	2819.0
28	3148.5
29	3478.0
30	4490.0
31	5502.0
32	6604.5
33	7707.0
34	8382.0
35	9057.0
36	9938.5
37	10820.0
38	11935.5
39	13051.0
40	15842.0
41	18633.0
42	25554.0
43	32475.0
44	45440.5
45	58406.0
46	58453.5
47	58501.0
48	55541.0
49	52581.0
50	47074.0
51	41567.0
52	36944.0
53	32321.0
54	28726.0
55	25131.0
56	22332.5
57	19534.0
58	18234.5
59	16935.0
60	16908.5
61	16882.0
62	15473.5
63	14065.0
64	12344.5
65	10624.0
66	8634.0
67	6644.0
68	5659.5
69	4675.0
70	4179.0
71	3683.0
72	2763.0
73	1843.0
74	1648.0
75	1139.5
76	826.0
77	726.0
78	626.0
79	442.0
80	258.0
81	206.5
82	155.0
83	117.0
84	79.0
85	82.5
86	86.0
87	55.5
88	25.0
89	24.5
90	24.0
91	15.0
92	6.0
93	5.0
94	4.0
95	3.0
96	2.0
97	2.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	489511.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.688909412637724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.04393594084239	21.88640381884324
2	9.211554471838301	5.101157946406174
3	3.1274173845613094	2.597843299698795
4	1.528503768962668	1.692904095827306
5	0.8591347753307246	1.1894252483689647
6	0.5271728275397649	0.8758104399931583
7	0.38218582284214697	0.7407616039229432
8	0.2987453160181247	0.6617545594140548
9	0.25815626568707917	0.6433258909422934
>10	3.274051006984134	22.551595164227685
>50	1.2088679670063047	23.79653349671231
>100	0.2692109871612576	9.96672065567061
>500	0.0036878217419350355	0.7158034526181893
>1k	0.0051629504387090495	2.815629729314401
>5k	0.0022126930451610216	4.7643305980398685
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCC	8011	1.6365311504746576	No Hit
AATCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCT	7704	1.5738155015924054	TruSeq Adapter, Index 3 (95% over 21bp)
AATGATACCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGT	7614	1.5554298064803447	No Hit
CTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGCT	4666	0.9531961488097306	TruSeq Adapter, Index 3 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2746	0.5609679864190998	No Hit
AATGACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTT	1656	0.33829679006191893	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCT	1462	0.29866540282036563	No Hit
AAACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCT	1114	0.2275740483870638	RNA PCR Primer, Index 24 (95% over 21bp)
AACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTG	1088	0.2222626253546907	RNA PCR Primer, Index 3 (95% over 22bp)
AATGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTC	1055	0.21552120381360174	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTAT	900	0.18385695112060813	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	683	0.13952699735041704	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTT	683	0.13952699735041704	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	668	0.13646271483174024	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	571	0.1166470212109636	RNA PCR Primer, Index 3 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	498	0.10173417962006982	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.0857100249024026E-4	0.0	0.0	1.241442991066595	0.0
2	4.0857100249024026E-4	0.0	0.0	1.5666655090488262	0.0
3	4.0857100249024026E-4	0.0	0.0	2.168694881218195	0.0
4	4.0857100249024026E-4	0.0	0.0	5.3898686648512495	0.0
5	4.0857100249024026E-4	0.0	0.0	5.889959571899303	0.0
6	4.0857100249024026E-4	0.0	0.0	7.510760738778087	0.0
7	4.0857100249024026E-4	0.0	0.0	8.764869430921879	0.0
8	6.128565037353603E-4	0.0	0.0	9.596107135488273	0.0
9	6.128565037353603E-4	0.0	0.0	12.245281515634991	0.0
10	6.128565037353603E-4	0.0	0.0	13.76577850140242	0.0
11	6.128565037353603E-4	0.0	0.0	16.915656645100928	0.0
12	6.128565037353603E-4	0.0	0.0	17.770182896809263	0.0
13	8.171420049804805E-4	0.0	0.0	18.23881383666557	0.0
14	8.171420049804805E-4	0.0	0.0	18.798760395578444	0.0
15	8.171420049804805E-4	0.0	0.0	19.118671490528303	0.0
16	8.171420049804805E-4	0.0	0.0	19.709669445630436	0.0
17	8.171420049804805E-4	0.0	0.0	20.338460218462917	0.0
18	8.171420049804805E-4	0.0	0.0	21.469589038857144	0.0
19	8.171420049804805E-4	0.0	0.0	21.824637240021165	0.0
20	8.171420049804805E-4	0.0	0.0	22.225649678965336	0.0
21	8.171420049804805E-4	0.0	0.0	22.607867851794953	0.0
22	8.171420049804805E-4	0.0	0.0	22.978237465552358	0.0
23	8.171420049804805E-4	0.0	0.0	23.370465627942988	0.0
24	8.171420049804805E-4	0.0	0.0	23.638896776579077	0.0
25	8.171420049804805E-4	0.0	0.0	23.857686548412598	0.0
26	8.171420049804805E-4	0.0	0.0	24.075659178241143	0.0
27	8.171420049804805E-4	0.0	0.0	24.353487459934506	0.0
28	8.171420049804805E-4	0.0	0.0	24.587598644361414	0.0
29	8.171420049804805E-4	0.0	0.0	24.848471229451434	0.0
30	8.171420049804805E-4	0.0	0.0	25.26174079847031	0.0
31	8.171420049804805E-4	0.0	0.0	25.519344815540407	0.0
32	8.171420049804805E-4	0.0	0.0	25.761423134515873	0.0
33	8.171420049804805E-4	0.0	0.0	25.999824314468928	0.0
34	8.171420049804805E-4	0.0	0.0	26.269889747114977	0.0
35	8.171420049804805E-4	0.0	0.0	26.552008024334487	0.0
36	8.171420049804805E-4	0.0	0.0	26.782850640741476	0.0
37	8.171420049804805E-4	0.0	0.0	27.02043467868955	0.0
38	8.171420049804805E-4	0.0	0.0	27.266394422188675	0.0
39	8.171420049804805E-4	0.0	0.0	27.512762736690288	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGT	30	2.1629385E-6	45.000004	36
CTTCGGT	20	7.0293125E-4	45.000004	24
TTTAGCG	35	1.21001E-7	45.000004	1
CCGGGTA	40	6.8012014E-9	45.000004	5
GGTACGA	30	2.1629385E-6	45.000004	30
ACACGTG	60	0.0	45.000004	42
TCGCAAG	20	7.0293125E-4	45.000004	1
GCGACGC	20	7.0293125E-4	45.000004	25
TACTTTA	20	7.0293125E-4	45.000004	38
CGTATCT	20	7.0293125E-4	45.000004	42
GATCGGA	20	7.0293125E-4	45.000004	21
TTCACCG	20	7.0293125E-4	45.000004	1
CACGAGA	20	7.0293125E-4	45.000004	24
TCTAGTC	20	7.0293125E-4	45.000004	32
TCTAGCG	35	1.21001E-7	45.000004	1
TTTCAAT	20	7.0293125E-4	45.000004	45
CGTTACG	20	7.0293125E-4	45.000004	1
GTTACGG	80	0.0	45.000004	2
TCGGAAT	20	7.0293125E-4	45.000004	9
GTTCGTG	30	2.1629385E-6	45.000004	12
>>END_MODULE